JP7799325B2 - タンパク質足場 - Google Patents

タンパク質足場

Info

Publication number
JP7799325B2
JP7799325B2 JP2022580351A JP2022580351A JP7799325B2 JP 7799325 B2 JP7799325 B2 JP 7799325B2 JP 2022580351 A JP2022580351 A JP 2022580351A JP 2022580351 A JP2022580351 A JP 2022580351A JP 7799325 B2 JP7799325 B2 JP 7799325B2
Authority
JP
Japan
Prior art keywords
seq
scaffold
loop
randomized
regions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022580351A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023541764A5 (https=
JP2023541764A (ja
Inventor
ロドリー・フィリップ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neko Pharma
Original Assignee
Neko Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neko Pharma filed Critical Neko Pharma
Publication of JP2023541764A publication Critical patent/JP2023541764A/ja
Publication of JP2023541764A5 publication Critical patent/JP2023541764A5/ja
Application granted granted Critical
Publication of JP7799325B2 publication Critical patent/JP7799325B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022580351A 2020-06-25 2021-05-27 タンパク質足場 Active JP7799325B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/911,398 US11566346B2 (en) 2020-06-25 2020-06-25 Protein scaffold
US16/911,398 2020-06-25
PCT/NZ2021/050086 WO2021262012A2 (en) 2020-06-25 2021-05-27 Protein scaffold

Publications (3)

Publication Number Publication Date
JP2023541764A JP2023541764A (ja) 2023-10-04
JP2023541764A5 JP2023541764A5 (https=) 2024-04-04
JP7799325B2 true JP7799325B2 (ja) 2026-01-15

Family

ID=79032483

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022580351A Active JP7799325B2 (ja) 2020-06-25 2021-05-27 タンパク質足場

Country Status (5)

Country Link
US (2) US11566346B2 (https=)
EP (1) EP4172389A4 (https=)
JP (1) JP7799325B2 (https=)
CN (1) CN115997051A (https=)
WO (1) WO2021262012A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024118635A2 (en) * 2022-11-28 2024-06-06 Nectagen, Inc. Thermostable binding scaffolds
WO2025096668A1 (en) * 2023-10-30 2025-05-08 The Board Of Trustees Of The Leland Stanford Junior University System and compositions comprising mhc scaffold and methods of identifying antigens using the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011501951A (ja) 2007-10-31 2011-01-20 メディミューン，エルエルシー タンパク質足場

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19742706B4 (de) 1997-09-26 2013-07-25 Pieris Proteolab Ag Lipocalinmuteine
US8663650B2 (en) 2003-02-21 2014-03-04 Ac Immune Sa Methods and compositions comprising supramolecular constructs
JP6440496B2 (ja) * 2011-09-27 2018-12-19 ヤンセン バイオテツク，インコーポレーテツド 選択的結合表面を有するフィブロネクチンｉｉｉ型反復ベースのタンパク質スカフォールド
ES2984405T3 (es) 2012-09-13 2024-10-29 Bristol Myers Squibb Co Proteínas de dominio de andamio basadas en fibronectina que se unen a la miostatina
US10370776B2 (en) 2013-09-25 2019-08-06 Idea Orchard, Llc Antibody like protein
WO2020089235A1 (en) * 2018-10-29 2020-05-07 ETH Zürich Assay for screening of recombinant cells and microbes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011501951A (ja) 2007-10-31 2011-01-20 メディミューン，エルエルシー タンパク質足場

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Biochemical and Biophysical Research Communications，2011年，Vol.411，p.69-75
PNAS，2009年，Vol.106, No.14，p.5865-5870

Also Published As

Publication number Publication date
US20230250557A1 (en) 2023-08-10
WO2021262012A2 (en) 2021-12-30
EP4172389A4 (en) 2024-10-30
EP4172389A2 (en) 2023-05-03
US20210403900A1 (en) 2021-12-30
CN115997051A (zh) 2023-04-21
WO2021262012A3 (en) 2022-02-03
JP2023541764A (ja) 2023-10-04
US12404606B2 (en) 2025-09-02
US11566346B2 (en) 2023-01-31

Similar Documents

Publication Publication Date Title
JP7221259B2 (ja) 改変した抗体組成物、それを作製および使用する方法
JP7252278B2 (ja) 活性化可能な結合ポリペプチドおよびその同定方法ならびに使用
ES2361409T3 (es) Generación de proteinas de unión sintéticas basadas en proteinas de ubiquitina.
ES2306794T3 (es) Moleculas de autoensamblaje.
US9492572B2 (en) Dimeric binding proteins based on modified ubiquitins
KR102406610B1 (ko) 혈액-뇌 장벽을 통과이동하는 인간화 항체 및 그의 용도
JP2017079797A (ja) 免疫グロブリンドメインの工学的作製
US12404606B2 (en) Protein scaffold
JP2005512962A (ja) 抗ｐｄｇｆ抗体および設計抗体の産生方法
JP2026031936A (ja) 線維芽細胞活性化タンパク質（ｆａｐ）のための特異的結合性分子
WO2022148491A1 (zh) 一种可组装蛋白原件及其用途
CN117580858A (zh) 多价蛋白质及筛选方法
JP2025534992A (ja) 新規なジスルフィド架橋を有する超安定抗体断片
CN115667307A (zh) 合成的单域文库
Smith Advances in Phage Display—A Perspective
Afsharnoori et al. Isolation and characterization of a novel single-chain variable fragment (scFv) against lymphocyte function-associated antigen-1 (LFA-1) using phage display method
JP2015062367A (ja) Ｒｏｂｏ１タンパク質に対するダイアボディ型二重特異性抗体
Lee et al. Construction of an Enhanced Phage Display Library of Artificially Randomized vNARs from Cloudy Catshark (Scyliorhinus Torazame) and Screening against Miamiensis Avidus
Han Exploring Composition of Peptide Loops to Enhance Biophysical Properties of Antibody Fragments for Cancer Therapeutics
CN121969745A (zh) 多肽的聚集体形成抑制方法
Park mRNA Display selection of a novel activated leukocyte cell adhesion molecule (ALCAM) binding protein from a modified combinatorial protein library based on the tenth domain of human fibronection III (10FnIII)
Deen Isolation of Human Endoplasmic Reticulum Antibodies Using Phage Display Technology

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230818

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240325

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240325

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250416

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250616

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250903

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20251009

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20251203

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20251219

R150 Certificate of patent or registration of utility model

Ref document number: 7799325

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150