JP7766262B2 - 分泌されたタンパク質の製造方法 - Google Patents

分泌されたタンパク質の製造方法

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Publication number
JP7766262B2
JP7766262B2 JP2021558356A JP2021558356A JP7766262B2 JP 7766262 B2 JP7766262 B2 JP 7766262B2 JP 2021558356 A JP2021558356 A JP 2021558356A JP 2021558356 A JP2021558356 A JP 2021558356A JP 7766262 B2 JP7766262 B2 JP 7766262B2
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Prior art keywords
protein
outer membrane
cell wall
proteins
cyanobacterium
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JP2021558356A
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English (en)
Japanese (ja)
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JPWO2021100640A5 (https=
JPWO2021100640A1 (https=
Inventor
征司 児島
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Panasonic Intellectual Property Management Co Ltd
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Panasonic Intellectual Property Management Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021558356A 2019-11-21 2020-11-16 分泌されたタンパク質の製造方法 Active JP7766262B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019210114 2019-11-21
JP2019210114 2019-11-21
PCT/JP2020/042549 WO2021100640A1 (ja) 2019-11-21 2020-11-16 改変シアノバクテリア、改変シアノバクテリアの製造方法、及び、タンパク質の製造方法

Publications (3)

Publication Number Publication Date
JPWO2021100640A1 JPWO2021100640A1 (https=) 2021-05-27
JPWO2021100640A5 JPWO2021100640A5 (https=) 2022-07-21
JP7766262B2 true JP7766262B2 (ja) 2025-11-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021558356A Active JP7766262B2 (ja) 2019-11-21 2020-11-16 分泌されたタンパク質の製造方法

Country Status (5)

Country Link
US (1) US20220325312A1 (https=)
EP (1) EP4063483A4 (https=)
JP (1) JP7766262B2 (https=)
CN (1) CN114651060A (https=)
WO (1) WO2021100640A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114901830A (zh) * 2019-12-23 2022-08-12 松下知识产权经营株式会社 植物生长促进剂的制造方法、植物生长促进剂及植物生长促进方法
MX2023010047A (es) * 2021-03-04 2023-09-05 Panasonic Ip Man Co Ltd Cianobacterias modificadas, metodo de produccion de cianobacterias modificadas y metodo de produccion de proteinas.
JPWO2023195367A1 (https=) 2022-04-08 2023-10-12
JPWO2023233996A1 (https=) * 2022-05-31 2023-12-07
JPWO2023248690A1 (https=) * 2022-06-20 2023-12-28
EP4563707A4 (en) 2022-07-26 2025-12-10 Panasonic Ip Man Co Ltd METHOD FOR DETERMINING THE DETACHMENT OF THE OUTER MEMBRANE OF A CYANOBACTERIA, DEVICE FOR DETERMINING THE DETACHMENT OF THE OUTER MEMBRANE OF A CYANOBACTERIA AND PROGRAM
CN121693339A (zh) * 2023-08-23 2026-03-17 松下知识产权经营株式会社 抗氧化剂、活性氧的除去方法和抗氧化剂的制造方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59141383A (ja) 1983-02-03 1984-08-14 Mitsubishi Heavy Ind Ltd 円筒胴体製造法
JP5688665B2 (ja) 2011-10-18 2015-03-25 国立大学法人茨城大学 自己溶菌能を有するシアノバクテリアを用いたバイオ燃料等有用物質の製造方法
AU2015368667A1 (en) * 2014-12-22 2017-07-13 Kao Corporation Modified cyanobacteria
EP4063485A4 (en) * 2019-11-21 2023-07-19 Panasonic Intellectual Property Management Co., Ltd. ELECTRON CARRIER, METHOD OF MAKING AN ELECTRON CARRIER AND METHOD OF ELECTRON TRANSFER
WO2021100642A1 (ja) * 2019-11-21 2021-05-27 パナソニックＩｐマネジメント株式会社 改変シアノバクテリア、改変シアノバクテリアの製造方法、及び、タンパク質の製造方法
CN114901830A (zh) * 2019-12-23 2022-08-12 松下知识产权经营株式会社 植物生长促进剂的制造方法、植物生长促进剂及植物生长促进方法
CN116600636A (zh) * 2020-12-25 2023-08-15 松下知识产权经营株式会社 植物酸性转化酶活化剂的制造方法、植物酸性转化酶活化剂及植物酸性转化酶活化方法
JP2022134906A (ja) * 2021-03-04 2022-09-15 パナソニックＩｐマネジメント株式会社 バイオフィルムの製造方法、バイオフィルム、及び有機物の製造方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
児島征司，葉緑体表層膜で機能する細菌由来の膜安定化機構と物質透過機構の解明と応用，科研費, [オンライン]，2018年04月23日，[検索日2020.12.14], インターネット: <URL: https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18H02117/>
木幡光，Studies on molecular basis of cyanobacterial outer membrane function and its evolutionary relationsh，博士論文, [オンライン]，2018年03月27日，[検索日2020.12.14], インターネット: <URL: http://hdl.handle.net/10097/00122689>

Also Published As

Publication number Publication date
EP4063483A1 (en) 2022-09-28
US20220325312A1 (en) 2022-10-13
EP4063483A4 (en) 2023-12-20
CN114651060A (zh) 2022-06-21
JPWO2021100640A1 (https=) 2021-05-27
WO2021100640A1 (ja) 2021-05-27

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