JP7741351B2 - 遺伝子操作された微生物 - Google Patents

遺伝子操作された微生物

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Publication number
JP7741351B2
JP7741351B2 JP2022533350A JP2022533350A JP7741351B2 JP 7741351 B2 JP7741351 B2 JP 7741351B2 JP 2022533350 A JP2022533350 A JP 2022533350A JP 2022533350 A JP2022533350 A JP 2022533350A JP 7741351 B2 JP7741351 B2 JP 7741351B2
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Japan
Prior art keywords
gene
aspects
cellulose
bacterium
microorganism
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JP2022533350A
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English (en)
Japanese (ja)
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JP2023505193A (ja
JP2023505193A5 (enExample
Inventor
マルホール，ロス
Original Assignee
クロバイオ リミテッド
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Priority claimed from GBGB1917651.0A external-priority patent/GB201917651D0/en
Priority claimed from GBGB2016372.1A external-priority patent/GB202016372D0/en
Application filed by クロバイオ リミテッド filed Critical クロバイオ リミテッド
Publication of JP2023505193A publication Critical patent/JP2023505193A/ja
Publication of JP2023505193A5 publication Critical patent/JP2023505193A5/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022533350A 2019-12-03 2020-12-03 遺伝子操作された微生物 Active JP7741351B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1917651.0A GB201917651D0 (en) 2019-12-03 2019-12-03 Genetically engineered bacteria
GB1917651.0 2019-12-03
GB2016372.1 2020-10-15
GBGB2016372.1A GB202016372D0 (en) 2020-10-15 2020-10-15 Genetically engineered microorganisims
PCT/EP2020/084505 WO2021110856A1 (en) 2019-12-03 2020-12-03 Genetically engineered microorganisms

Publications (3)

Publication Number Publication Date
JP2023505193A JP2023505193A (ja) 2023-02-08
JP2023505193A5 JP2023505193A5 (enExample) 2023-12-11
JP7741351B2 true JP7741351B2 (ja) 2025-09-18

Family

ID=73726822

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022533350A Active JP7741351B2 (ja) 2019-12-03 2020-12-03 遺伝子操作された微生物

Country Status (7)

Country Link
US (1) US20230034438A1 (enExample)
EP (1) EP4069859A1 (enExample)
JP (1) JP7741351B2 (enExample)
CN (1) CN115038792A (enExample)
AU (1) AU2020394834A1 (enExample)
BR (1) BR112022010303A2 (enExample)
WO (1) WO2021110856A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116064353B (zh) * 2022-10-19 2024-08-23 山东大学 一株多效产酶溶杆菌基因工程菌及其构建方法与应用
GB202313342D0 (en) 2023-09-01 2023-10-18 Crobio Ltd Engineered bacteria for improving soil quality
WO2025174810A2 (en) 2024-02-12 2025-08-21 Switch Bioworks, Inc. Density-dependent microbial gene switch

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014104310A1 (ja) 2012-12-28 2014-07-03 国立大学法人京都大学 セルロース製造用発現ベクター、形質転換体およびセルロースの製造方法
WO2019084342A1 (en) 2017-10-25 2019-05-02 Pivot Bio, Inc. GENETIC TARGETS FOR TARGETING NITROGEN FIXATION FOR IMPROVING PLANT CHARACTERISTICS
WO2019084059A2 (en) 2017-10-25 2019-05-02 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR ENHANCING GENETICALLY MODIFIED MICROBES THAT FIX NITROGEN

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0016842D0 (en) * 2000-07-07 2000-08-30 Isis Innovation Bacterial polysaccharide
KR20180060833A (ko) * 2016-11-29 2018-06-07 삼성전자주식회사 셀룰로스 신타아제의 활성을 증가시키는 유전적 변형을 포함하는 재조합 미생물 및 그를 이용한 셀룰로스를 생산하는 방법
CN108060112B (zh) 2017-11-28 2019-09-10 南京工业大学 一株细菌纤维素生产菌株及其构建方法与应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014104310A1 (ja) 2012-12-28 2014-07-03 国立大学法人京都大学 セルロース製造用発現ベクター、形質転換体およびセルロースの製造方法
WO2019084342A1 (en) 2017-10-25 2019-05-02 Pivot Bio, Inc. GENETIC TARGETS FOR TARGETING NITROGEN FIXATION FOR IMPROVING PLANT CHARACTERISTICS
WO2019084059A2 (en) 2017-10-25 2019-05-02 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR ENHANCING GENETICALLY MODIFIED MICROBES THAT FIX NITROGEN

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Andrew J. et al.，Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads，Cellulose - Medical, Pharmaceutical and Electronic Applications，2013年，Chapter: 1，(pp. 1-26)
Gizem BULDUM et al.，Bioprocess and Biosystems Engineering，2018年，Vol. 41，pp. 265-279
Lindsey NIELSEN et al.，Environmental Microbiology，2011年，Vol. 13，pp. 1342-1356

Also Published As

Publication number Publication date
BR112022010303A2 (pt) 2022-08-16
CN115038792A (zh) 2022-09-09
AU2020394834A1 (en) 2022-07-21
WO2021110856A1 (en) 2021-06-10
EP4069859A1 (en) 2022-10-12
JP2023505193A (ja) 2023-02-08
US20230034438A1 (en) 2023-02-02

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