JP7728744B2 - バニリンの高効率生成のための改変宿主細胞 - Google Patents

バニリンの高効率生成のための改変宿主細胞

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Publication number
JP7728744B2
JP7728744B2 JP2022502919A JP2022502919A JP7728744B2 JP 7728744 B2 JP7728744 B2 JP 7728744B2 JP 2022502919 A JP2022502919 A JP 2022502919A JP 2022502919 A JP2022502919 A JP 2022502919A JP 7728744 B2 JP7728744 B2 JP 7728744B2
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vanillin
host cell
certain embodiments
genetically modified
glucovanillin
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JP2022542228A (ja
JP2022542228A5 (cg-RX-API-DMAC7.html
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レェツ，ローレン
ハンセン，チャド
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アミリス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00—Compounds having —CHO groups
    • C07C47/52—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings
    • C07C47/575—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing ether groups, groups, groups, or groups
    • C07C47/58—Vanillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003—Aldehyde dehydrogenase (NAD+) (1.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2022502919A 2019-08-01 2020-07-31 バニリンの高効率生成のための改変宿主細胞 Active JP7728744B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962881874P 2019-08-01 2019-08-01
US62/881,874 2019-08-01
PCT/US2020/044613 WO2021022216A1 (en) 2019-08-01 2020-07-31 Modified host cells for high efficiency production of vanillin

Publications (3)

Publication Number Publication Date
JP2022542228A JP2022542228A (ja) 2022-09-30
JP2022542228A5 JP2022542228A5 (cg-RX-API-DMAC7.html) 2023-08-29
JP7728744B2 true JP7728744B2 (ja) 2025-08-25

Family

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Family Applications (1)

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JP2022502919A Active JP7728744B2 (ja) 2019-08-01 2020-07-31 バニリンの高効率生成のための改変宿主細胞

Country Status (8)

Country Link
US (1) US12480101B2 (cg-RX-API-DMAC7.html)
EP (1) EP4007815A1 (cg-RX-API-DMAC7.html)
JP (1) JP7728744B2 (cg-RX-API-DMAC7.html)
CN (1) CN114174489B (cg-RX-API-DMAC7.html)
BR (1) BR112021025372A2 (cg-RX-API-DMAC7.html)
CA (1) CA3147424A1 (cg-RX-API-DMAC7.html)
MX (1) MX2022001073A (cg-RX-API-DMAC7.html)
WO (1) WO2021022216A1 (cg-RX-API-DMAC7.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230175023A1 (en) * 2021-02-18 2023-06-08 Zymergen Inc. Engineered biosynthetic pathways for production of 3,4-dihydroxybenzoic acid by fermentation
WO2023159017A1 (en) 2022-02-15 2023-08-24 Evolva Sa Process for recovering and purifying vanillin
CN114790472B (zh) * 2022-03-25 2024-04-16 上海龙殷生物科技有限公司 一种果糖糖基化的姜黄素、制备方法及应用
CN117004544B (zh) * 2023-09-14 2023-12-12 北京化工大学 一种以葡萄糖为底物从头合成香兰素的基因工程菌及其应用
KR20250098104A (ko) * 2023-12-21 2025-07-01 대상 주식회사 1,4-부탄디올 생산능이 향상된 변이 미생물 및 이를 이용한 1,4-부탄디올의 생산 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2344665A1 (en) 1998-09-18 2000-03-30 Board Of Trustees Operating Michigan State University Synthesis of vanillin from a carbon source
US20140017748A1 (en) * 2010-02-12 2014-01-16 Gevo, Inc. Modified alcohol dehydrogenases for the production of fuels and chemicals
CN108570464B (zh) * 2011-08-08 2021-12-21 国际香料香精公司 用于香草醛或香草醛β-D-葡萄糖苷的生物合成的组合物和方法
US10000782B2 (en) * 2013-07-16 2018-06-19 International Flavors & Fragrances Inc. Recombinant host cell for the biosynthesis of vanillin or vanillin beta-D-glucoside
BR112016002526B1 (pt) 2013-08-07 2021-11-23 Total Marketing Services Método para produção de um composto heterólogo não catabólico, e, composição de fermentação
US20170172184A1 (en) 2014-02-12 2017-06-22 Evolva Sa Methods of Improving Production of Vanillin
EP2957629A1 (en) * 2014-06-18 2015-12-23 Rhodia Opérations Improved production of vanilloids by fermentation
WO2016044713A1 (en) * 2014-09-18 2016-03-24 Genomatica, Inc. Non-natural microbial organisms with improved energetic efficiency
EP4134442A1 (en) 2015-05-29 2023-02-15 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph
US10808015B2 (en) 2015-06-25 2020-10-20 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Nature communications，2016年，Vol.7, No.1，Articlenumber: 11709

Also Published As

Publication number Publication date
MX2022001073A (es) 2022-02-14
CA3147424A1 (en) 2021-02-04
JP2022542228A (ja) 2022-09-30
CN114174489A (zh) 2022-03-11
US12480101B2 (en) 2025-11-25
WO2021022216A1 (en) 2021-02-04
US20220282226A1 (en) 2022-09-08
BR112021025372A2 (pt) 2022-02-08
CN114174489B (zh) 2024-11-08
EP4007815A1 (en) 2022-06-08

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