CN114174489B - 用于高效生成香兰素的经修饰宿主细胞 - Google Patents

用于高效生成香兰素的经修饰宿主细胞 Download PDF

Info

Publication number
CN114174489B
CN114174489B CN202080055048.6A CN202080055048A CN114174489B CN 114174489 B CN114174489 B CN 114174489B CN 202080055048 A CN202080055048 A CN 202080055048A CN 114174489 B CN114174489 B CN 114174489B
Authority
CN
China
Prior art keywords
vanillin
host cell
genetically modified
glucovanillin
modified host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080055048.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN114174489A (zh
Inventor
L·雷茨
C·汉森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of CN114174489A publication Critical patent/CN114174489A/zh
Application granted granted Critical
Publication of CN114174489B publication Critical patent/CN114174489B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00—Compounds having —CHO groups
    • C07C47/52—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings
    • C07C47/575—Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing ether groups, groups, groups, or groups
    • C07C47/58—Vanillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003—Aldehyde dehydrogenase (NAD+) (1.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CN202080055048.6A 2019-08-01 2020-07-31 用于高效生成香兰素的经修饰宿主细胞 Active CN114174489B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962881874P 2019-08-01 2019-08-01
US62/881874 2019-08-01
PCT/US2020/044613 WO2021022216A1 (en) 2019-08-01 2020-07-31 Modified host cells for high efficiency production of vanillin

Publications (2)

Publication Number Publication Date
CN114174489A CN114174489A (zh) 2022-03-11
CN114174489B true CN114174489B (zh) 2024-11-08

Family

ID=72179198

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080055048.6A Active CN114174489B (zh) 2019-08-01 2020-07-31 用于高效生成香兰素的经修饰宿主细胞

Country Status (8)

Country Link
US (1) US12480101B2 (cg-RX-API-DMAC7.html)
EP (1) EP4007815A1 (cg-RX-API-DMAC7.html)
JP (1) JP7728744B2 (cg-RX-API-DMAC7.html)
CN (1) CN114174489B (cg-RX-API-DMAC7.html)
BR (1) BR112021025372A2 (cg-RX-API-DMAC7.html)
CA (1) CA3147424A1 (cg-RX-API-DMAC7.html)
MX (1) MX2022001073A (cg-RX-API-DMAC7.html)
WO (1) WO2021022216A1 (cg-RX-API-DMAC7.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230175023A1 (en) * 2021-02-18 2023-06-08 Zymergen Inc. Engineered biosynthetic pathways for production of 3,4-dihydroxybenzoic acid by fermentation
WO2023159017A1 (en) 2022-02-15 2023-08-24 Evolva Sa Process for recovering and purifying vanillin
CN114790472B (zh) * 2022-03-25 2024-04-16 上海龙殷生物科技有限公司 一种果糖糖基化的姜黄素、制备方法及应用
CN117004544B (zh) * 2023-09-14 2023-12-12 北京化工大学 一种以葡萄糖为底物从头合成香兰素的基因工程菌及其应用
KR20250098104A (ko) * 2023-12-21 2025-07-01 대상 주식회사 1,4-부탄디올 생산능이 향상된 변이 미생물 및 이를 이용한 1,4-부탄디올의 생산 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2957629A1 (en) * 2014-06-18 2015-12-23 Rhodia Opérations Improved production of vanilloids by fermentation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2344665A1 (en) 1998-09-18 2000-03-30 Board Of Trustees Operating Michigan State University Synthesis of vanillin from a carbon source
US20140017748A1 (en) * 2010-02-12 2014-01-16 Gevo, Inc. Modified alcohol dehydrogenases for the production of fuels and chemicals
CN108570464B (zh) * 2011-08-08 2021-12-21 国际香料香精公司 用于香草醛或香草醛β-D-葡萄糖苷的生物合成的组合物和方法
US10000782B2 (en) * 2013-07-16 2018-06-19 International Flavors & Fragrances Inc. Recombinant host cell for the biosynthesis of vanillin or vanillin beta-D-glucoside
BR112016002526B1 (pt) 2013-08-07 2021-11-23 Total Marketing Services Método para produção de um composto heterólogo não catabólico, e, composição de fermentação
US20170172184A1 (en) 2014-02-12 2017-06-22 Evolva Sa Methods of Improving Production of Vanillin
WO2016044713A1 (en) * 2014-09-18 2016-03-24 Genomatica, Inc. Non-natural microbial organisms with improved energetic efficiency
EP4134442A1 (en) 2015-05-29 2023-02-15 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph
US10808015B2 (en) 2015-06-25 2020-10-20 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2957629A1 (en) * 2014-06-18 2015-12-23 Rhodia Opérations Improved production of vanilloids by fermentation

Also Published As

Publication number Publication date
MX2022001073A (es) 2022-02-14
CA3147424A1 (en) 2021-02-04
JP2022542228A (ja) 2022-09-30
JP7728744B2 (ja) 2025-08-25
CN114174489A (zh) 2022-03-11
US12480101B2 (en) 2025-11-25
WO2021022216A1 (en) 2021-02-04
US20220282226A1 (en) 2022-09-08
BR112021025372A2 (pt) 2022-02-08
EP4007815A1 (en) 2022-06-08

Similar Documents

Publication Publication Date Title
CN114174489B (zh) 用于高效生成香兰素的经修饰宿主细胞
US20250327107A1 (en) Pisum sativum kaurene oxidase for high efficiency production of rebaudiosides
US12460239B2 (en) UDP-glycosyltransferase variants and uses thereof
JP2020526213A (ja) エクトイン産生酵母
US11608488B2 (en) Enhanced metabolite-producing yeast
JP7834088B2 (ja) バニリンの高収率生成のための培養組成物及びその使用方法
US20250257381A1 (en) Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
US20240175066A1 (en) Modified host cells for high efficiency production of vanillin
JP7518838B2 (ja) レバウジオシドの高効率な生成のためのａｂｃトランスポーター
US12516357B1 (en) Modified host cells for high efficiency production of vanillin
US20220049279A1 (en) Malonic semi-aldehyde-producing yeasts
KR20250130385A (ko) 세스퀴테르페노이드를 생산할 수 있는 숙주 세포 및 이의 사용 방법

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant