JP7708408B2 - 二本鎖ｄｎａ合成方法 - Google Patents

二本鎖ｄｎａ合成方法

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Publication number
JP7708408B2
JP7708408B2 JP2018093024A JP2018093024A JP7708408B2 JP 7708408 B2 JP7708408 B2 JP 7708408B2 JP 2018093024 A JP2018093024 A JP 2018093024A JP 2018093024 A JP2018093024 A JP 2018093024A JP 7708408 B2 JP7708408 B2 JP 7708408B2
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JP
Japan
Prior art keywords
double
stranded dna
dna
stranded
seconds
Prior art date
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Application number
JP2018093024A
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English (en)
Japanese (ja)
Other versions
JP2019198236A5 (cg-RX-API-DMAC7.html
JP2019198236A (ja
Inventor
俊介 高橋
謙爾 柘植
昭彦 近藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kobe University NUC
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Kobe University NUC
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Publication date
Application filed by Kobe University NUC filed Critical Kobe University NUC
Priority to JP2018093024A priority Critical patent/JP7708408B2/ja
Priority to US16/185,106 priority patent/US20190345527A1/en
Publication of JP2019198236A publication Critical patent/JP2019198236A/ja
Publication of JP2019198236A5 publication Critical patent/JP2019198236A5/ja
Priority to JP2023029982A priority patent/JP2023060013A/ja
Priority to JP2025114665A priority patent/JP2025129399A/ja
Application granted granted Critical
Publication of JP7708408B2 publication Critical patent/JP7708408B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2018093024A 2018-05-14 2018-05-14 二本鎖ｄｎａ合成方法 Active JP7708408B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2018093024A JP7708408B2 (ja) 2018-05-14 2018-05-14 二本鎖ｄｎａ合成方法
US16/185,106 US20190345527A1 (en) 2018-05-14 2018-11-09 Method for synthesizing double-stranded dna
JP2023029982A JP2023060013A (ja) 2018-05-14 2023-02-28 二本鎖ｄｎａ合成方法
JP2025114665A JP2025129399A (ja) 2018-05-14 2025-07-07 二本鎖ｄｎａ合成方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2018093024A JP7708408B2 (ja) 2018-05-14 2018-05-14 二本鎖ｄｎａ合成方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023029982A Division JP2023060013A (ja) 2018-05-14 2023-02-28 二本鎖ｄｎａ合成方法

Publications (3)

Publication Number Publication Date
JP2019198236A JP2019198236A (ja) 2019-11-21
JP2019198236A5 JP2019198236A5 (cg-RX-API-DMAC7.html) 2021-06-10
JP7708408B2 true JP7708408B2 (ja) 2025-07-15

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018093024A Active JP7708408B2 (ja) 2018-05-14 2018-05-14 二本鎖ｄｎａ合成方法
JP2023029982A Pending JP2023060013A (ja) 2018-05-14 2023-02-28 二本鎖ｄｎａ合成方法
JP2025114665A Pending JP2025129399A (ja) 2018-05-14 2025-07-07 二本鎖ｄｎａ合成方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2023029982A Pending JP2023060013A (ja) 2018-05-14 2023-02-28 二本鎖ｄｎａ合成方法
JP2025114665A Pending JP2025129399A (ja) 2018-05-14 2025-07-07 二本鎖ｄｎａ合成方法

Country Status (2)

Country Link
US (1) US20190345527A1 (cg-RX-API-DMAC7.html)
JP (3) JP7708408B2 (cg-RX-API-DMAC7.html)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116970667A (zh) * 2022-04-24 2023-10-31 深圳华大生命科学研究院 一种基于固相载体的单链dna的合成与扩增方法
WO2023214579A1 (ja) 2022-05-02 2023-11-09 株式会社シンプロジェン ウイルス由来構築物プラスミド
JPWO2023214578A1 (cg-RX-API-DMAC7.html) 2022-05-02 2023-11-09
CN121358846A (zh) 2023-06-09 2026-01-16 新博基股份有限公司 菌株的物质生产

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080182296A1 (en) 2007-01-31 2008-07-31 Chanda Pranab K Pcr-directed gene synthesis from large number of overlapping oligodeoxyribonucleotides
JP2013099332A (ja) 2003-03-12 2013-05-23 Primeradx Inc リアルタイム遺伝子発現プロフィール解析
JP2015536689A (ja) 2012-12-13 2015-12-24 シンセティック ゲノミクス、インク． Ｐｅｇを介した核酸分子のアセンブリ

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8263392B2 (en) * 2006-11-14 2012-09-11 University Of Utah Research Foundation Methods and compositions related to continuous flow thermal gradient PCR
SG175963A1 (en) * 2009-05-11 2011-12-29 Agency Science Tech & Res Gene synthesis method
GB2487632B (en) * 2011-01-14 2014-03-12 Genefirst Ltd Methods,compositions,and kits for determining the presence/absence of a variant nucleic acid sequence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013099332A (ja) 2003-03-12 2013-05-23 Primeradx Inc リアルタイム遺伝子発現プロフィール解析
US20080182296A1 (en) 2007-01-31 2008-07-31 Chanda Pranab K Pcr-directed gene synthesis from large number of overlapping oligodeoxyribonucleotides
JP2015536689A (ja) 2012-12-13 2015-12-24 シンセティック ゲノミクス、インク． Ｐｅｇを介した核酸分子のアセンブリ

Also Published As

Publication number Publication date
JP2025129399A (ja) 2025-09-04
JP2023060013A (ja) 2023-04-27
JP2019198236A (ja) 2019-11-21
US20190345527A1 (en) 2019-11-14

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