JP7696368B2 - タバコ植物及びたばこ製品 - Google Patents

タバコ植物及びたばこ製品 Download PDF

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Publication number
JP7696368B2
JP7696368B2 JP2022568325A JP2022568325A JP7696368B2 JP 7696368 B2 JP7696368 B2 JP 7696368B2 JP 2022568325 A JP2022568325 A JP 2022568325A JP 2022568325 A JP2022568325 A JP 2022568325A JP 7696368 B2 JP7696368 B2 JP 7696368B2
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JP
Japan
Prior art keywords
amino acid
tobacco
acid sequence
nitrate reductase
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022568325A
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English (en)
Japanese (ja)
Other versions
JPWO2022124361A1 (https=
JPWO2022124361A5 (https=
Inventor
庄一 鈴木
龍 岡田
総一郎 野口
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Publication of JPWO2022124361A1 publication Critical patent/JPWO2022124361A1/ja
Publication of JPWO2022124361A5 publication Critical patent/JPWO2022124361A5/ja
Application granted granted Critical
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Classifications

    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01001—Nitrate reductase (NADH) (1.7.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
JP2022568325A 2020-12-09 2021-12-09 タバコ植物及びたばこ製品 Active JP7696368B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020203916 2020-12-09
JP2020203916 2020-12-09
PCT/JP2021/045295 WO2022124361A1 (ja) 2020-12-09 2021-12-09 タバコ植物及びたばこ製品

Publications (3)

Publication Number Publication Date
JPWO2022124361A1 JPWO2022124361A1 (https=) 2022-06-16
JPWO2022124361A5 JPWO2022124361A5 (https=) 2023-08-28
JP7696368B2 true JP7696368B2 (ja) 2025-06-20

Family

ID=81973308

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022568325A Active JP7696368B2 (ja) 2020-12-09 2021-12-09 タバコ植物及びたばこ製品

Country Status (5)

Country Link
US (1) US20230320309A1 (https=)
EP (1) EP4260685A4 (https=)
JP (1) JP7696368B2 (https=)
CN (1) CN116583176B (https=)
WO (1) WO2022124361A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118475234A (zh) 2021-12-27 2024-08-09 日本烟草产业株式会社 烟草植物
WO2026057635A1 (en) * 2024-09-12 2026-03-19 Philip Morris Products S.A. Modulation of nitrate levels in tobacco via mutation of nitrate reductase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015197727A2 (en) 2014-06-25 2015-12-30 Philip Morris Products S.A Modulation of nitrate content in plants
WO2016046288A1 (en) 2014-09-26 2016-03-31 Philip Morris Products S.A Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
WO2020141062A1 (en) 2018-12-30 2020-07-09 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108004242B (zh) * 2017-12-21 2021-07-13 河南农业大学 烟草硝酸还原酶nia1启动子、其表达载体及其应用
CN108004241B (zh) * 2017-12-21 2021-07-09 河南农业大学 烟草硝酸还原酶nia2启动子、其表达载体及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015197727A2 (en) 2014-06-25 2015-12-30 Philip Morris Products S.A Modulation of nitrate content in plants
WO2016046288A1 (en) 2014-09-26 2016-03-31 Philip Morris Products S.A Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
WO2020141062A1 (en) 2018-12-30 2020-07-09 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase

Also Published As

Publication number Publication date
WO2022124361A1 (ja) 2022-06-16
JPWO2022124361A1 (https=) 2022-06-16
EP4260685A1 (en) 2023-10-18
US20230320309A1 (en) 2023-10-12
CN116583176A (zh) 2023-08-11
CN116583176B (zh) 2024-12-31
EP4260685A4 (en) 2024-11-20

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