JP7569372B2 - デュアルインデックス付きメチル－ｓｅｑライブラリーの調製方法 - Google Patents

デュアルインデックス付きメチル－ｓｅｑライブラリーの調製方法 Download PDF

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JP7569372B2
JP7569372B2 JP2022517788A JP2022517788A JP7569372B2 JP 7569372 B2 JP7569372 B2 JP 7569372B2 JP 2022517788 A JP2022517788 A JP 2022517788A JP 2022517788 A JP2022517788 A JP 2022517788A JP 7569372 B2 JP7569372 B2 JP 7569372B2
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dna
adaptor
adapter
ligase
double
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JP2022551401A (ja
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ダス・チャクラバルティー，ウシャティ
ファン，シャオ－ユン
ツェン，ユー
ライ，ケビン
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インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022517788A 2019-09-30 2020-09-29 デュアルインデックス付きメチル－ｓｅｑライブラリーの調製方法 Active JP7569372B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962907778P 2019-09-30 2019-09-30
US62/907,778 2019-09-30
PCT/US2020/053284 WO2021067275A1 (en) 2019-09-30 2020-09-29 Methods of preparing dual indexed methyl-seq libraries

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JP2022551401A JP2022551401A (ja) 2022-12-09
JP2022551401A5 JP2022551401A5 (https=) 2023-04-18
JP7569372B2 true JP7569372B2 (ja) 2024-10-17

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JP2022517788A Active JP7569372B2 (ja) 2019-09-30 2020-09-29 デュアルインデックス付きメチル－ｓｅｑライブラリーの調製方法

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US (1) US20210095351A1 (https=)
EP (1) EP4038200A4 (https=)
JP (1) JP7569372B2 (https=)
CN (1) CN114555831B (https=)
AU (1) AU2020359506A1 (https=)
CA (1) CA3147326A1 (https=)
WO (1) WO2021067275A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4083231A1 (en) 2020-07-30 2022-11-02 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
WO2022255944A2 (en) * 2021-06-02 2022-12-08 Lucence Life Sciences Pte. Ltd. Method for detection and quantification of methylated dna
CN114032288A (zh) * 2021-12-10 2022-02-11 北京吉因加医学检验实验室有限公司 一种试剂盒及其制备测序用靶核苷酸的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140329697A1 (en) 2011-11-15 2014-11-06 Bgi Tech Solutions Co., Ltd. Constructing method of high-throughput sequencing library and use thereof
WO2018140695A1 (en) 2017-01-27 2018-08-02 Integrated Dna Technologies, Inc. Construction of next generation sequencing (ngs) libraries using competitive strand displacement
JP2019504645A (ja) 2016-02-08 2019-02-21 アールジーン・インコーポレイテッドＲｇｅｎｅ， Ｉｎｃ． 複数のリガーゼ組成物、システム、および方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
CN101802223A (zh) * 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11566284B2 (en) * 2016-08-10 2023-01-31 Grail, Llc Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140329697A1 (en) 2011-11-15 2014-11-06 Bgi Tech Solutions Co., Ltd. Constructing method of high-throughput sequencing library and use thereof
JP2019504645A (ja) 2016-02-08 2019-02-21 アールジーン・インコーポレイテッドＲｇｅｎｅ， Ｉｎｃ． 複数のリガーゼ組成物、システム、および方法
WO2018140695A1 (en) 2017-01-27 2018-08-02 Integrated Dna Technologies, Inc. Construction of next generation sequencing (ngs) libraries using competitive strand displacement

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
RAINE, Amanda et al.，Nucleic Acids Research，2017年04月07日，Vol. 45, Issue 6, Article No. e36，DOI: 10.1093/nar/gkw1110
SCHUMACHER, Cassie et al.，Nature Methods，2015年10月29日，Vol. 12，pp. v-vi，DOI: 10.1038/nmeth.f.386
URICH, Mark A. et al.，Nature Protocols，2015年02月18日，Vol. 10，pp. 475-483，DOI: 10.1038/nprot.2014.114.

Also Published As

Publication number Publication date
CN114555831A (zh) 2022-05-27
AU2020359506A1 (en) 2022-03-10
CA3147326A1 (en) 2021-04-08
EP4038200A4 (en) 2023-01-18
CN114555831B (zh) 2025-10-14
JP2022551401A (ja) 2022-12-09
US20210095351A1 (en) 2021-04-01
EP4038200A1 (en) 2022-08-10
WO2021067275A1 (en) 2021-04-08

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