CA3147326A1 - Methods of preparing dual indexed methyl-seq libraries - Google Patents
Methods of preparing dual indexed methyl-seq libraries Download PDFInfo
- Publication number
- CA3147326A1 CA3147326A1 CA3147326A CA3147326A CA3147326A1 CA 3147326 A1 CA3147326 A1 CA 3147326A1 CA 3147326 A CA3147326 A CA 3147326A CA 3147326 A CA3147326 A CA 3147326A CA 3147326 A1 CA3147326 A1 CA 3147326A1
- Authority
- CA
- Canada
- Prior art keywords
- adapter
- dna
- target
- nucleic acid
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962907778P | 2019-09-30 | 2019-09-30 | |
| US62/907,778 | 2019-09-30 | ||
| PCT/US2020/053284 WO2021067275A1 (en) | 2019-09-30 | 2020-09-29 | Methods of preparing dual indexed methyl-seq libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3147326A1 true CA3147326A1 (en) | 2021-04-08 |
Family
ID=75161759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3147326A Pending CA3147326A1 (en) | 2019-09-30 | 2020-09-29 | Methods of preparing dual indexed methyl-seq libraries |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20210095351A1 (https=) |
| EP (1) | EP4038200A4 (https=) |
| JP (1) | JP7569372B2 (https=) |
| CN (1) | CN114555831B (https=) |
| AU (1) | AU2020359506A1 (https=) |
| CA (1) | CA3147326A1 (https=) |
| WO (1) | WO2021067275A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4083231A1 (en) | 2020-07-30 | 2022-11-02 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
| WO2022255944A2 (en) * | 2021-06-02 | 2022-12-08 | Lucence Life Sciences Pte. Ltd. | Method for detection and quantification of methylated dna |
| CN114032288A (zh) * | 2021-12-10 | 2022-02-11 | 北京吉因加医学检验实验室有限公司 | 一种试剂盒及其制备测序用靶核苷酸的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008096146A1 (en) * | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| CN101802223A (zh) * | 2007-08-15 | 2010-08-11 | 香港大学 | 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途 |
| US20100273164A1 (en) * | 2009-03-24 | 2010-10-28 | President And Fellows Of Harvard College | Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation |
| CN103103624B (zh) * | 2011-11-15 | 2014-12-31 | 深圳华大基因科技服务有限公司 | 高通量测序文库的构建方法及其应用 |
| US20150011396A1 (en) * | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| AU2017218431B2 (en) * | 2016-02-08 | 2022-08-04 | RGENE, Inc. | Multiple ligase compositions, systems, and methods |
| US11566284B2 (en) * | 2016-08-10 | 2023-01-31 | Grail, Llc | Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing |
| AU2018212756B2 (en) * | 2017-01-27 | 2021-02-25 | Integrated Dna Technologies, Inc. | Construction of next generation sequencing (NGS) libraries using competitive strand displacement |
-
2020
- 2020-09-29 JP JP2022517788A patent/JP7569372B2/ja active Active
- 2020-09-29 WO PCT/US2020/053284 patent/WO2021067275A1/en not_active Ceased
- 2020-09-29 CA CA3147326A patent/CA3147326A1/en active Pending
- 2020-09-29 EP EP20872889.9A patent/EP4038200A4/en active Pending
- 2020-09-29 CN CN202080067258.7A patent/CN114555831B/zh active Active
- 2020-09-29 AU AU2020359506A patent/AU2020359506A1/en active Pending
- 2020-09-29 US US17/036,986 patent/US20210095351A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN114555831A (zh) | 2022-05-27 |
| AU2020359506A1 (en) | 2022-03-10 |
| JP7569372B2 (ja) | 2024-10-17 |
| EP4038200A4 (en) | 2023-01-18 |
| CN114555831B (zh) | 2025-10-14 |
| JP2022551401A (ja) | 2022-12-09 |
| US20210095351A1 (en) | 2021-04-01 |
| EP4038200A1 (en) | 2022-08-10 |
| WO2021067275A1 (en) | 2021-04-08 |
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|---|---|---|---|
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