CN114555831B - 制备双索引甲基化序列文库的方法 - Google Patents

制备双索引甲基化序列文库的方法

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Publication number
CN114555831B
CN114555831B CN202080067258.7A CN202080067258A CN114555831B CN 114555831 B CN114555831 B CN 114555831B CN 202080067258 A CN202080067258 A CN 202080067258A CN 114555831 B CN114555831 B CN 114555831B
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China
Prior art keywords
dna
nucleic acid
target
target nucleic
sequencing
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CN202080067258.7A
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English (en)
Chinese (zh)
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CN114555831A (zh
Inventor
乌沙提·达斯查卡雷维尔提
黄晓芸
郑钰
凯文·莱
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Integrated DNA Technologies Inc
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Integrated DNA Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080067258.7A 2019-09-30 2020-09-29 制备双索引甲基化序列文库的方法 Active CN114555831B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962907778P 2019-09-30 2019-09-30
US62/907,778 2019-09-30
PCT/US2020/053284 WO2021067275A1 (en) 2019-09-30 2020-09-29 Methods of preparing dual indexed methyl-seq libraries

Publications (2)

Publication Number Publication Date
CN114555831A CN114555831A (zh) 2022-05-27
CN114555831B true CN114555831B (zh) 2025-10-14

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CN202080067258.7A Active CN114555831B (zh) 2019-09-30 2020-09-29 制备双索引甲基化序列文库的方法

Country Status (7)

Country Link
US (1) US20210095351A1 (https=)
EP (1) EP4038200A4 (https=)
JP (1) JP7569372B2 (https=)
CN (1) CN114555831B (https=)
AU (1) AU2020359506A1 (https=)
CA (1) CA3147326A1 (https=)
WO (1) WO2021067275A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4083231A1 (en) 2020-07-30 2022-11-02 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
WO2022255944A2 (en) * 2021-06-02 2022-12-08 Lucence Life Sciences Pte. Ltd. Method for detection and quantification of methylated dna
CN114032288A (zh) * 2021-12-10 2022-02-11 北京吉因加医学检验实验室有限公司 一种试剂盒及其制备测序用靶核苷酸的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
CN101802223A (zh) * 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
US20100273164A1 (en) * 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
CN103103624B (zh) * 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
AU2017218431B2 (en) * 2016-02-08 2022-08-04 RGENE, Inc. Multiple ligase compositions, systems, and methods
US11566284B2 (en) * 2016-08-10 2023-01-31 Grail, Llc Methods of preparing dual-indexed DNA libraries for bisulfite conversion sequencing
AU2018212756B2 (en) * 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing;Mark A Urich等;nature protocols;20150218;第10卷(第3期);摘要，图1 *
Two are better than one: HPoxBS - hairpin oxidative bisulfite sequencing;Pascal Giehr等;Nucleic Acids Research;20180615;第46卷(第15期);摘要、第3页第3-4段、第5页第3段 *

Also Published As

Publication number Publication date
CN114555831A (zh) 2022-05-27
AU2020359506A1 (en) 2022-03-10
JP7569372B2 (ja) 2024-10-17
CA3147326A1 (en) 2021-04-08
EP4038200A4 (en) 2023-01-18
JP2022551401A (ja) 2022-12-09
US20210095351A1 (en) 2021-04-01
EP4038200A1 (en) 2022-08-10
WO2021067275A1 (en) 2021-04-08

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