JP7549759B1 - 蛍光画像取得方法、蛍光画像取得装置、及び蛍光画像取得プログラム - Google Patents

蛍光画像取得方法、蛍光画像取得装置、及び蛍光画像取得プログラム Download PDF

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JP7549759B1
JP7549759B1 JP2024539324A JP2024539324A JP7549759B1 JP 7549759 B1 JP7549759 B1 JP 7549759B1 JP 2024539324 A JP2024539324 A JP 2024539324A JP 2024539324 A JP2024539324 A JP 2024539324A JP 7549759 B1 JP7549759 B1 JP 7549759B1
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fluorescence
image
fluorescent
pixel
images
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JPWO2024176618A5 (https=
JPWO2024176618A1 (https=
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貴文 樋口
賢一郎 池村
航 加茂
なつみ 加藤
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Hamamatsu Photonics KK
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Analysis (AREA)
JP2024539324A 2023-02-24 2023-12-26 蛍光画像取得方法、蛍光画像取得装置、及び蛍光画像取得プログラム Active JP7549759B1 (ja)

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JP2024148540A JP2024163162A (ja) 2023-02-24 2024-08-30 蛍光画像取得方法、蛍光画像取得装置、及び蛍光画像取得プログラム

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JP2023027521 2023-02-24
JP2023027521 2023-02-24
PCT/JP2023/046787 WO2024176618A1 (ja) 2023-02-24 2023-12-26 蛍光画像取得方法、蛍光画像取得装置、及び蛍光画像取得プログラム

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005036143A1 (ja) * 2003-10-10 2005-04-21 Hamamatsu Photonics K.K. 蛍光色素の濃度を定量する方法およびシステム
JP2008541179A (ja) * 2005-05-18 2008-11-20 オリンパス・ソフト・イメージング・ソリューションズ・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング 多色画像、特に顕微鏡多色透過画像におけるスペクトル重畳またはカラー重畳画像寄与の分離
US20210208076A1 (en) * 2020-01-03 2021-07-08 Korea Advanced Institute Of Science And Technology Method and Apparatus for Multiplexed Imaging of Spectrally-Similar Fluorophores
WO2023026742A1 (ja) * 2021-08-25 2023-03-02 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005036143A1 (ja) * 2003-10-10 2005-04-21 Hamamatsu Photonics K.K. 蛍光色素の濃度を定量する方法およびシステム
JP2008541179A (ja) * 2005-05-18 2008-11-20 オリンパス・ソフト・イメージング・ソリューションズ・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング 多色画像、特に顕微鏡多色透過画像におけるスペクトル重畳またはカラー重畳画像寄与の分離
US20210208076A1 (en) * 2020-01-03 2021-07-08 Korea Advanced Institute Of Science And Technology Method and Apparatus for Multiplexed Imaging of Spectrally-Similar Fluorophores
WO2023026742A1 (ja) * 2021-08-25 2023-03-02 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MACRE, Tristan D. et al.，Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning，PLOS ONE，2019年12月02日，1-24
MACRE, TRISTAN D. ET AL.: "Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning", PLOS ONE, JPN7022004308, 2 December 2019 (2019-12-02), pages 1 - 24, ISSN: 0005388600 *

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JPWO2024176618A1 (https=) 2024-08-29
JP2024163162A (ja) 2024-11-21
EP4653849A1 (en) 2025-11-26
CN120731357A (zh) 2025-09-30

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