JP7535376B2 - 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット - Google Patents

核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット Download PDF

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JP7535376B2
JP7535376B2 JP2019540367A JP2019540367A JP7535376B2 JP 7535376 B2 JP7535376 B2 JP 7535376B2 JP 2019540367 A JP2019540367 A JP 2019540367A JP 2019540367 A JP2019540367 A JP 2019540367A JP 7535376 B2 JP7535376 B2 JP 7535376B2
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library
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JP2020508973A (ja
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ボスティック，マグノリア
マン，イシュマインダー
アラン ファーマー，アンドリュー
テイラー，サラ
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タカラ バイオ ユーエスエー， インコーポレイテッド
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

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JP2019540367A 2017-02-16 2018-02-13 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット Active JP7535376B2 (ja)

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JP2022177448A JP2023011863A (ja) 2017-02-16 2022-11-04 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット
JP2024127839A JP2024156876A (ja) 2017-02-16 2024-08-02 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット

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US201762459858P 2017-02-16 2017-02-16
US62/459,858 2017-02-16
PCT/US2018/018057 WO2018152129A1 (en) 2017-02-16 2018-02-13 Methods of preparing nucleic acid libraries and compositions and kits for practicing the same

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JP7535376B2 true JP7535376B2 (ja) 2024-08-16

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JP2022177448A Pending JP2023011863A (ja) 2017-02-16 2022-11-04 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット
JP2024127839A Pending JP2024156876A (ja) 2017-02-16 2024-08-02 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット

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JP2024127839A Pending JP2024156876A (ja) 2017-02-16 2024-08-02 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット

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US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2017214417A1 (en) 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
JP7535376B2 (ja) * 2017-02-16 2024-08-16 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019191459A1 (en) * 2018-03-28 2019-10-03 Berkeley Lights, Inc. Methods for preparation of nucleic acid sequencing libraries
US20210079459A1 (en) * 2018-05-01 2021-03-18 Takara Bio Usa, Inc. Methods of Amplifying Nucleic Acids and Compositions and Kits for Practicing the Same
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3902922A1 (en) * 2018-12-28 2021-11-03 Biobloxx AB Method and kit for preparing complementary dna
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
CN110241460A (zh) * 2019-05-31 2019-09-17 南方医科大学南方医院 一种甄别独立样品自身交叉反应的免疫组库方法
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021188384A1 (en) * 2020-03-14 2021-09-23 Berkeley Lights, Inc. Methods of amplifying paired transcript sequences from single cells
EP4150118A1 (en) * 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
WO2023122309A1 (en) * 2021-12-23 2023-06-29 Takara Bio Usa, Inc. Methods and compositions for producing cell-source identifiable collections of nucleic acids

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JP6338221B2 (ja) 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
EP3760733A1 (en) * 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
AU2015253299B2 (en) * 2014-04-29 2018-06-14 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
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JP7535376B2 (ja) * 2017-02-16 2024-08-16 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット

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CN110199022A (zh) 2019-09-03
EP3583212A1 (en) 2019-12-25
EP3583212A4 (en) 2021-01-13
JP2023011863A (ja) 2023-01-24
JP2024156876A (ja) 2024-11-06
JP2020508973A (ja) 2020-03-26
US12065644B2 (en) 2024-08-20
WO2018152129A1 (en) 2018-08-23
US20200339978A1 (en) 2020-10-29
US20250027075A1 (en) 2025-01-23

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