CN110199022A - 制备核酸文库的方法和用于实施所述方法的组合物和试剂盒 - Google Patents

制备核酸文库的方法和用于实施所述方法的组合物和试剂盒 Download PDF

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Publication number
CN110199022A
CN110199022A CN201880006993.XA CN201880006993A CN110199022A CN 110199022 A CN110199022 A CN 110199022A CN 201880006993 A CN201880006993 A CN 201880006993A CN 110199022 A CN110199022 A CN 110199022A
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China
Prior art keywords
template
nucleic acid
primer
cdna
library
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Pending
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CN201880006993.XA
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English (en)
Chinese (zh)
Inventor
马格诺利娅·博斯蒂克
伊施明德尔·曼恩
安德鲁·艾伦·法默
萨拉·泰勒
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Bao Bioengineering (usa) Co Ltd
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Bao Bioengineering (usa) Co Ltd
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Publication of CN110199022A publication Critical patent/CN110199022A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201880006993.XA 2017-02-16 2018-02-13 制备核酸文库的方法和用于实施所述方法的组合物和试剂盒 Pending CN110199022A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762459858P 2017-02-16 2017-02-16
US62/459,858 2017-02-16
PCT/US2018/018057 WO2018152129A1 (en) 2017-02-16 2018-02-13 Methods of preparing nucleic acid libraries and compositions and kits for practicing the same

Publications (1)

Publication Number Publication Date
CN110199022A true CN110199022A (zh) 2019-09-03

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880006993.XA Pending CN110199022A (zh) 2017-02-16 2018-02-13 制备核酸文库的方法和用于实施所述方法的组合物和试剂盒

Country Status (5)

Country Link
US (2) US12065644B2 (enExample)
EP (1) EP3583212A4 (enExample)
JP (3) JP7535376B2 (enExample)
CN (1) CN110199022A (enExample)
WO (1) WO2018152129A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2017214417A1 (en) 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
JP7535376B2 (ja) * 2017-02-16 2024-08-16 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019191459A1 (en) * 2018-03-28 2019-10-03 Berkeley Lights, Inc. Methods for preparation of nucleic acid sequencing libraries
US20210079459A1 (en) * 2018-05-01 2021-03-18 Takara Bio Usa, Inc. Methods of Amplifying Nucleic Acids and Compositions and Kits for Practicing the Same
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3902922A1 (en) * 2018-12-28 2021-11-03 Biobloxx AB Method and kit for preparing complementary dna
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
CN110241460A (zh) * 2019-05-31 2019-09-17 南方医科大学南方医院 一种甄别独立样品自身交叉反应的免疫组库方法
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021188384A1 (en) * 2020-03-14 2021-09-23 Berkeley Lights, Inc. Methods of amplifying paired transcript sequences from single cells
EP4150118A1 (en) * 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
WO2023122309A1 (en) * 2021-12-23 2023-06-29 Takara Bio Usa, Inc. Methods and compositions for producing cell-source identifiable collections of nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7294712B2 (en) * 1990-06-04 2007-11-13 Aventis Pharma S.A. Nucleotide sequences coding for variable regions of β chains of human T lymphocyte receptors, corresponding peptide segments and the diagnostic and therapeutic uses
JP6338221B2 (ja) 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
EP3760733A1 (en) * 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
AU2015253299B2 (en) * 2014-04-29 2018-06-14 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
NZ730044A (en) 2014-09-15 2024-08-30 Abvitro Llc High-throughput nucleotide library sequencing
PL3289095T3 (pl) * 2015-04-29 2020-01-31 Mirdetect Gmbh Wykrywanie cząsteczek kwasu nukleinowego
EP3350732B1 (en) 2015-09-15 2024-07-24 Takara Bio USA, Inc. Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same
JP7535376B2 (ja) * 2017-02-16 2024-08-16 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸ライブラリーの調製方法ならびにその方法を行うための組成物及びキット

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EVGENY S. EGOROV: "Quantitative Profiling of Immune Repertoires for Minor Lymphocyte Counts Using Unique Molecular Identifiers", 《J IMMUNOL》 *
SIMONE PICELLI: "Full-length RNA-seq from single cells using Smart-seq2", 《NAT PROTOC》 *

Also Published As

Publication number Publication date
EP3583212A1 (en) 2019-12-25
EP3583212A4 (en) 2021-01-13
JP2023011863A (ja) 2023-01-24
JP2024156876A (ja) 2024-11-06
JP2020508973A (ja) 2020-03-26
US12065644B2 (en) 2024-08-20
WO2018152129A1 (en) 2018-08-23
US20200339978A1 (en) 2020-10-29
US20250027075A1 (en) 2025-01-23
JP7535376B2 (ja) 2024-08-16

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