JP7531502B2 - ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 - Google Patents

ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 Download PDF

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JP7531502B2
JP7531502B2 JP2021548194A JP2021548194A JP7531502B2 JP 7531502 B2 JP7531502 B2 JP 7531502B2 JP 2021548194 A JP2021548194 A JP 2021548194A JP 2021548194 A JP2021548194 A JP 2021548194A JP 7531502 B2 JP7531502 B2 JP 7531502B2
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labeled probe
subsets
probe
target
target nucleic
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マ，シャオ－チュン
チャン，ピンチン
ワン，リ－チョン
ルー，ハン
ワン，リ
ヅォン，ヘイリン
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アドヴァンスド セル ダイアグノスティクス，インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021548194A 2019-02-15 2020-02-14 ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 Active JP7531502B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962806574P 2019-02-15 2019-02-15
US62/806,574 2019-02-15
PCT/US2020/018241 WO2020168162A1 (en) 2019-02-15 2020-02-14 Methods for multiplex detection of nucleic acids by in situ hybridization

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JP2022520663A JP2022520663A (ja) 2022-03-31
JP2022520663A5 JP2022520663A5 (cg-RX-API-DMAC7.html) 2023-02-17
JP7531502B2 true JP7531502B2 (ja) 2024-08-09

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US (2) US12571028B2 (cg-RX-API-DMAC7.html)
EP (1) EP3924501A4 (cg-RX-API-DMAC7.html)
JP (1) JP7531502B2 (cg-RX-API-DMAC7.html)
CN (1) CN114555825A (cg-RX-API-DMAC7.html)
SG (1) SG11202108618VA (cg-RX-API-DMAC7.html)
WO (1) WO2020168162A1 (cg-RX-API-DMAC7.html)

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* Cited by examiner, † Cited by third party
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WO2020092796A1 (en) * 2018-11-01 2020-05-07 Advanced Cell Diagnostics, Inc. Methods for digital multiplexing of nucleic acids in situ
US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2022221637A1 (en) * 2021-04-16 2022-10-20 Advanced Cell Diagnostics, Inc. Methods and compositions for reducing autofluorescence
US12626364B2 (en) 2021-04-16 2026-05-12 Advanced Cell Diagnostics, Inc. Materials and methods related to image processing
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

Citations (2)

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WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US20180094305A1 (en) 2016-10-04 2018-04-05 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of rna in cells and tissues

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AU9220498A (en) * 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
EP3042963A1 (en) 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US20090081688A1 (en) 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
DE602007009487D1 (de) 2006-01-27 2010-11-11 Isis Pharmaceutical Inc 6-modifizierte bicyclische nukleinsäureanaloga
ES2562821T3 (es) 2010-10-21 2016-03-08 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
US20160046984A1 (en) 2014-08-15 2016-02-18 Affymetrix, Inc. Robust Detection of Nucleic Acids in Situ
EP3913068B1 (en) 2015-10-12 2023-08-02 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
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US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

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WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US20180094305A1 (en) 2016-10-04 2018-04-05 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of rna in cells and tissues

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Also Published As

Publication number Publication date
EP3924501A4 (en) 2022-11-23
US12571028B2 (en) 2026-03-10
CN114555825A (zh) 2022-05-27
WO2020168162A1 (en) 2020-08-20
US20220154262A1 (en) 2022-05-19
US20260110019A1 (en) 2026-04-23
EP3924501A1 (en) 2021-12-22
SG11202108618VA (en) 2021-09-29
JP2022520663A (ja) 2022-03-31

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