JP7499761B2 - 細孔 - Google Patents

細孔 Download PDF

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Publication number
JP7499761B2
JP7499761B2 JP2021524380A JP2021524380A JP7499761B2 JP 7499761 B2 JP7499761 B2 JP 7499761B2 JP 2021524380 A JP2021524380 A JP 2021524380A JP 2021524380 A JP2021524380 A JP 2021524380A JP 7499761 B2 JP7499761 B2 JP 7499761B2
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JP
Japan
Prior art keywords
protein
nanopore
csgg
csgf
pore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021524380A
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English (en)
Japanese (ja)
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JP2022518095A (ja
JP2022518095A5 (https=
Inventor
ルモー，ハン
デール ヴェレン，サンダー，エグベルト ヴァン
ゲルヴェン，ナニ ヴァン
ジャヤシンヘ，ラクマル，ニシャンタ
ウォレス，エリザベス，ジェーン
シン，パトリック，ラージ
ハンブリー，リチャード，ジョージ
ジョーダン，マイケル，ロバート
キルグール，ジョン，ジョセフ
ヘロン，アンドリュー，ジョン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
Original Assignee
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1818216.2A external-priority patent/GB201818216D0/en
Priority claimed from GBGB1819054.6A external-priority patent/GB201819054D0/en
Application filed by Vlaams Instituut voor Biotechnologie VIB, Vrije Universiteit Brussel VUB filed Critical Vlaams Instituut voor Biotechnologie VIB
Publication of JP2022518095A publication Critical patent/JP2022518095A/ja
Publication of JP2022518095A5 publication Critical patent/JP2022518095A5/ja
Priority to JP2024090579A priority Critical patent/JP2024133465A/ja
Application granted granted Critical
Publication of JP7499761B2 publication Critical patent/JP7499761B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021524380A 2018-11-08 2019-11-07 細孔 Active JP7499761B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024090579A JP2024133465A (ja) 2018-11-08 2024-06-04 細孔

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1818216.2A GB201818216D0 (en) 2018-11-08 2018-11-08 Pore
GB1818216.2 2018-11-08
GBGB1819054.6A GB201819054D0 (en) 2018-11-22 2018-11-22 Pore
GB1819054.6 2018-11-22
PCT/GB2019/053153 WO2020095052A1 (en) 2018-11-08 2019-11-07 Pore

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024090579A Division JP2024133465A (ja) 2018-11-08 2024-06-04 細孔

Publications (3)

Publication Number Publication Date
JP2022518095A JP2022518095A (ja) 2022-03-14
JP2022518095A5 JP2022518095A5 (https=) 2022-10-04
JP7499761B2 true JP7499761B2 (ja) 2024-06-14

Family

ID=68531572

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021524380A Active JP7499761B2 (ja) 2018-11-08 2019-11-07 細孔
JP2024090579A Pending JP2024133465A (ja) 2018-11-08 2024-06-04 細孔

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024090579A Pending JP2024133465A (ja) 2018-11-08 2024-06-04 細孔

Country Status (7)

Country Link
US (1) US20220056517A1 (https=)
EP (1) EP3877547A1 (https=)
JP (2) JP7499761B2 (https=)
CN (1) CN113195736A (https=)
AU (1) AU2019375476B2 (https=)
CA (1) CA3118808A1 (https=)
WO (1) WO2020095052A1 (https=)

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GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106038B (zh) 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔
WO2023060420A1 (zh) * 2021-10-12 2023-04-20 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
GB202211602D0 (en) * 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211607D0 (en) * 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250048551A (ko) * 2022-08-09 2025-04-09 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어
KR20250044691A (ko) * 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
WO2024078621A1 (zh) * 2022-10-14 2024-04-18 北京普译生物科技有限公司 一种pht纳米孔突变体蛋白及其应用
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
CN120303287A (zh) * 2022-12-28 2025-07-11 深圳华大生命科学研究院 孔蛋白单体、孔蛋白及其突变体和其应用
WO2024138425A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 一种新型纳米孔蛋白及其应用
CN120418274A (zh) * 2022-12-28 2025-08-01 深圳华大生命科学研究院 纳米孔蛋白及其应用
CN120282978A (zh) * 2022-12-29 2025-07-08 深圳华大生命科学研究院 纳米孔蛋白及其突变体和应用
CN117417418B (zh) * 2023-01-12 2024-07-19 北京普译生物科技有限公司 一种具有超高热稳定性的纳米孔突变体及其应用
CN115974984A (zh) * 2023-01-17 2023-04-18 南方科技大学 双门孔道蛋白、孔道蛋白突变体、核苷酸序列及其应用
WO2025236238A1 (zh) * 2024-05-16 2025-11-20 北京普译生物科技有限公司 一种纳米孔蛋白单体及其应用
WO2025255780A1 (zh) * 2024-06-13 2025-12-18 深圳华大生命科学研究院 纳米孔蛋白复合物、其构建方法及应用
WO2026055978A1 (zh) * 2024-09-14 2026-03-19 深圳华大生命科学研究院 纳米孔蛋白复合物及其应用

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2014506575A (ja) 2011-02-11 2014-03-17 オックスフォード ナノポール テクノロジーズ リミテッド 変異体細孔
JP2017527284A (ja) 2014-09-01 2017-09-21 ブイアイビー ブイゼットダブリュＶｉｂ Ｖｚｗ 変異体ポア
WO2018146491A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
KR20110125226A (ko) 2009-01-30 2011-11-18 옥스포드 나노포어 테크놀로지즈 리미티드 혼성화 링커
CN102405410B (zh) 2009-04-20 2014-06-25 牛津楠路珀尔科技有限公司 脂质双层传感器阵列
US9127313B2 (en) 2009-12-01 2015-09-08 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
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GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
AU2014270410B2 (en) 2013-05-24 2020-07-16 Illumina Cambridge Limited Pyrophosphorolytic sequencing
EP3137490B1 (en) * 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
WO2016166232A1 (en) * 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
CN117106038B (zh) * 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔

Patent Citations (3)

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JP2014506575A (ja) 2011-02-11 2014-03-17 オックスフォード ナノポール テクノロジーズ リミテッド 変異体細孔
JP2017527284A (ja) 2014-09-01 2017-09-21 ブイアイビー ブイゼットダブリュＶｉｂ Ｖｚｗ 変異体ポア
WO2018146491A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof

Non-Patent Citations (4)

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Angew. Chem. Int. Ed.，2009年，Vol. 49, No. 3，pp. 556-559，DOI: 10.1002/anie.200905483
NATURE COMMUNICATIONS，2018年07月19日，Vol. 9, No. 1，2823, pp. 1-9，DOI: 10.1038/s41467-018-05108-5
PHILOSOPHICAL TRANSACTIONS B，2017年，Vol. 372，20160230, pp. 1-11，http://dx.doi.org/10.1098/rstb.2016.0230
SCIENCE ADVANCES，2015年，Vol. 1, No. 11，E1500905, pp. 1-8，DOI: 10.1126/sciadv.1500905

Also Published As

Publication number Publication date
JP2024133465A (ja) 2024-10-02
AU2019375476A1 (en) 2021-06-03
US20220056517A1 (en) 2022-02-24
AU2019375476B2 (en) 2026-04-16
JP2022518095A (ja) 2022-03-14
CN113195736A (zh) 2021-07-30
EP3877547A1 (en) 2021-09-15
WO2020095052A1 (en) 2020-05-14
CA3118808A1 (en) 2020-05-14
WO2020095052A8 (en) 2021-05-14

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