CN113195736A - 孔 - Google Patents

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Publication number
CN113195736A
CN113195736A CN201980073675.XA CN201980073675A CN113195736A CN 113195736 A CN113195736 A CN 113195736A CN 201980073675 A CN201980073675 A CN 201980073675A CN 113195736 A CN113195736 A CN 113195736A
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CN
China
Prior art keywords
protein
nanopore
csgg
csgf
pore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980073675.XA
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English (en)
Chinese (zh)
Inventor
汉·瑞曼特
S·E·范德韦伦
纳尼·范格文
拉科马·尼尚萨·贾亚辛格
伊丽莎白·杰恩·华莱士
普拉提克·拉吉·辛格
理查德·乔治·汉布利
迈克尔·罗伯特·乔丹
约翰·约瑟夫·基尔戈
A·J·赫伦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Universite Libre de Bruxelles ULB
Oxford Nanopore Technology Co
Original Assignee
Vlaams Instituut voor Biotechnologie VIB
Universite Libre de Bruxelles ULB
Oxford Nanopore Technology Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1818216.2A external-priority patent/GB201818216D0/en
Priority claimed from GBGB1819054.6A external-priority patent/GB201819054D0/en
Application filed by Vlaams Instituut voor Biotechnologie VIB, Universite Libre de Bruxelles ULB, Oxford Nanopore Technology Co filed Critical Vlaams Instituut voor Biotechnologie VIB
Publication of CN113195736A publication Critical patent/CN113195736A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201980073675.XA 2018-11-08 2019-11-07 孔 Pending CN113195736A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1818216.2A GB201818216D0 (en) 2018-11-08 2018-11-08 Pore
GB1818216.2 2018-11-08
GBGB1819054.6A GB201819054D0 (en) 2018-11-22 2018-11-22 Pore
GB1819054.6 2018-11-22
PCT/GB2019/053153 WO2020095052A1 (en) 2018-11-08 2019-11-07 Pore

Publications (1)

Publication Number Publication Date
CN113195736A true CN113195736A (zh) 2021-07-30

Family

ID=68531572

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980073675.XA Pending CN113195736A (zh) 2018-11-08 2019-11-07 孔

Country Status (7)

Country Link
US (1) US20220056517A1 (https=)
EP (1) EP3877547A1 (https=)
JP (2) JP7499761B2 (https=)
CN (1) CN113195736A (https=)
AU (1) AU2019375476B2 (https=)
CA (1) CA3118808A1 (https=)
WO (1) WO2020095052A1 (https=)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023060420A1 (zh) * 2021-10-12 2023-04-20 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
CN117417418A (zh) * 2023-01-12 2024-01-19 北京普译生物科技有限公司 一种具有超高热稳定性的纳米孔突变体及其应用
CN117886907A (zh) * 2022-10-14 2024-04-16 北京普译生物科技有限公司 一种pht纳米孔突变体蛋白及其应用
WO2024138425A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 一种新型纳米孔蛋白及其应用
WO2024138565A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 纳米孔蛋白及其突变体和应用
WO2024138473A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 孔蛋白单体、孔蛋白及其突变体和其应用
WO2024138424A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 纳米孔蛋白及其应用
WO2024152432A1 (zh) * 2023-01-17 2024-07-25 南方科技大学 双门孔道蛋白、孔道蛋白突变体、核苷酸序列及其应用
WO2025236238A1 (zh) * 2024-05-16 2025-11-20 北京普译生物科技有限公司 一种纳米孔蛋白单体及其应用
WO2025255780A1 (zh) * 2024-06-13 2025-12-18 深圳华大生命科学研究院 纳米孔蛋白复合物、其构建方法及应用
WO2026055978A1 (zh) * 2024-09-14 2026-03-19 深圳华大生命科学研究院 纳米孔蛋白复合物及其应用

Families Citing this family (12)

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GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106038B (zh) 2017-06-30 2025-12-09 弗拉芒区生物技术研究所 新颖蛋白孔
GB202211602D0 (en) * 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211607D0 (en) * 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250048551A (ko) * 2022-08-09 2025-04-09 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어
KR20250044691A (ko) * 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores

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JP2014506575A (ja) * 2011-02-11 2014-03-17 オックスフォード ナノポール テクノロジーズ リミテッド 変異体細孔
CN106574300A (zh) * 2014-05-02 2017-04-19 牛津纳米孔技术公司 改善目标多核苷酸相对于跨膜孔移动的方法
CN107735686A (zh) * 2015-04-14 2018-02-23 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
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CN102405410B (zh) 2009-04-20 2014-06-25 牛津楠路珀尔科技有限公司 脂质双层传感器阵列
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KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
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CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
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JP2014506575A (ja) * 2011-02-11 2014-03-17 オックスフォード ナノポール テクノロジーズ リミテッド 変異体細孔
CN106574300A (zh) * 2014-05-02 2017-04-19 牛津纳米孔技术公司 改善目标多核苷酸相对于跨膜孔移动的方法
CN107735686A (zh) * 2015-04-14 2018-02-23 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔

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KHERIM WILLEMS，ET AL: "single molecule nanopore enzymology", 《PHILOSOPHICAL TRANSACTIONS》, vol. 372, no. 1726, pages 3 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023060420A1 (zh) * 2021-10-12 2023-04-20 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
CN117886907A (zh) * 2022-10-14 2024-04-16 北京普译生物科技有限公司 一种pht纳米孔突变体蛋白及其应用
WO2024138425A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 一种新型纳米孔蛋白及其应用
WO2024138473A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 孔蛋白单体、孔蛋白及其突变体和其应用
WO2024138424A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 纳米孔蛋白及其应用
WO2024138565A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 纳米孔蛋白及其突变体和应用
CN117417418A (zh) * 2023-01-12 2024-01-19 北京普译生物科技有限公司 一种具有超高热稳定性的纳米孔突变体及其应用
WO2024152432A1 (zh) * 2023-01-17 2024-07-25 南方科技大学 双门孔道蛋白、孔道蛋白突变体、核苷酸序列及其应用
WO2025236238A1 (zh) * 2024-05-16 2025-11-20 北京普译生物科技有限公司 一种纳米孔蛋白单体及其应用
WO2025255780A1 (zh) * 2024-06-13 2025-12-18 深圳华大生命科学研究院 纳米孔蛋白复合物、其构建方法及应用
WO2026055978A1 (zh) * 2024-09-14 2026-03-19 深圳华大生命科学研究院 纳米孔蛋白复合物及其应用

Also Published As

Publication number Publication date
JP2024133465A (ja) 2024-10-02
AU2019375476A1 (en) 2021-06-03
US20220056517A1 (en) 2022-02-24
AU2019375476B2 (en) 2026-04-16
JP2022518095A (ja) 2022-03-14
JP7499761B2 (ja) 2024-06-14
EP3877547A1 (en) 2021-09-15
WO2020095052A1 (en) 2020-05-14
CA3118808A1 (en) 2020-05-14
WO2020095052A8 (en) 2021-05-14

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Address after: oxford

Applicant after: Oxford nanopore Technology Public Co.,Ltd.

Applicant after: VIB VZW

Applicant after: UNIVERSITE LIBRE DE BRUXELLES

Address before: Oxfordshire

Applicant before: Oxford nanopore technology Co.

Applicant before: VIB VZW

Applicant before: UNIVERSITE LIBRE DE BRUXELLES