JP7369393B2 - 非破壊的な細胞解析方法 - Google Patents
非破壊的な細胞解析方法 Download PDFInfo
- Publication number
- JP7369393B2 JP7369393B2 JP2019024097A JP2019024097A JP7369393B2 JP 7369393 B2 JP7369393 B2 JP 7369393B2 JP 2019024097 A JP2019024097 A JP 2019024097A JP 2019024097 A JP2019024097 A JP 2019024097A JP 7369393 B2 JP7369393 B2 JP 7369393B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- present
- medium
- analysis method
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
1.プラスティックへ接着し培養可能であること
2.細胞表面マーカーの発現について、CD73, CD90, CD105が陽性、CD45, CD34, CD14 or CD11b, CD79a or CD19, HLA-DRが陰性であること
3.骨芽細胞、脂肪細胞、軟骨細胞への分化能を有すること
糖鎖結合性タンパク質を固定化したマイクロアレイを用いて、培地中に細胞から分泌される機能因子からその糖鎖プロファイルを取得し、解析する方法。
前記細胞がヒト細胞である[1]の解析方法。
糖鎖プロファイルを取得する工程がエバネッセント波蛍光励起装置を用いて行われる[1]又は[2]の解析方法。
前記培地が無血清培地である[1]乃至[3]のいずれかの解析方法。
前記機能因子が糖タンパク質である[1]乃至[4]のいずれかの解析方法。
y = (log(x + 1.0) - log(xmin + 1.0)) / (log(xmax + 1.0) - log(xmin + 1.0))
Claims (2)
- 糖鎖結合性タンパク質を固定化したマイクロアレイを用いて、培地中にヒト細胞から分泌される糖タンパク質からその糖鎖プロファイルをエバネッセント波蛍光励起装置を用いて取得し、解析することで90%を超える認識精度で間葉系幹細胞であることを識別する細胞種の判定を行う方法。
- 前記培地が無血清培地である請求項1記載の方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019024097A JP7369393B2 (ja) | 2019-02-14 | 2019-02-14 | 非破壊的な細胞解析方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019024097A JP7369393B2 (ja) | 2019-02-14 | 2019-02-14 | 非破壊的な細胞解析方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2020134179A JP2020134179A (ja) | 2020-08-31 |
JP7369393B2 true JP7369393B2 (ja) | 2023-10-26 |
Family
ID=72278380
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019024097A Active JP7369393B2 (ja) | 2019-02-14 | 2019-02-14 | 非破壊的な細胞解析方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP7369393B2 (ja) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009148236A (ja) | 2007-12-20 | 2009-07-09 | Glycoresearch Kk | 線維芽細胞の細胞識別方法 |
US20120282628A1 (en) | 2011-05-06 | 2012-11-08 | Korea Basic Science Institute | Method for diagnosing cancer using lectin |
JP2017184713A (ja) | 2016-03-31 | 2017-10-12 | 国立研究開発法人産業技術総合研究所 | 細胞分化ポテンシャル判定法 |
-
2019
- 2019-02-14 JP JP2019024097A patent/JP7369393B2/ja active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009148236A (ja) | 2007-12-20 | 2009-07-09 | Glycoresearch Kk | 線維芽細胞の細胞識別方法 |
US20120282628A1 (en) | 2011-05-06 | 2012-11-08 | Korea Basic Science Institute | Method for diagnosing cancer using lectin |
JP2017184713A (ja) | 2016-03-31 | 2017-10-12 | 国立研究開発法人産業技術総合研究所 | 細胞分化ポテンシャル判定法 |
Non-Patent Citations (1)
Title |
---|
SHIMODA, A. et al.,Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells,Biochemical and Biophysical Research Communications,2017年,Vol.491,pp.701-707 |
Also Published As
Publication number | Publication date |
---|---|
JP2020134179A (ja) | 2020-08-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Ashcroft et al. | Determination of the size distribution of blood microparticles directly in plasma using atomic force microscopy and microfluidics | |
US9822399B2 (en) | Method for analyzing biomolecules and biomolecule analyzer | |
CN107615062A (zh) | 具有可调光学性质的水凝胶颗粒以及使用其的方法 | |
Zhang et al. | Morphology-based prediction of cancer cell migration using an artificial neural network and a random decision forest | |
Maley et al. | Simplified digital enzyme-linked immunosorbent assay using tyramide signal amplification and fibrin hydrogels | |
Klangprapan et al. | Sensing the classical swine fever virus with molecularly imprinted polymer on quartz crystal microbalance | |
Maglio et al. | A quartz crystal microbalance immunosensor for stem cell selection and extraction | |
CN102313814A (zh) | 一种基于纳米金增强的多种肺癌标志物的高灵敏检测方法 | |
Bahadır et al. | A comparative study of short chain and long chain mercapto acids used in biosensor fabrication: A VEGF-R1-based immunosensor as a model system | |
CN105861295A (zh) | 一种检测鼠伤寒沙门氏菌的生物传感器及制备、检测方法 | |
Liu et al. | A nanoparticle-based affinity sensor that identifies and selects highly cytokine-secreting cells | |
Tran et al. | Simple Label‐Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus | |
Lee et al. | Automated estimation of cancer cell deformability with machine learning and acoustic trapping | |
JP7369393B2 (ja) | 非破壊的な細胞解析方法 | |
JP2003000223A (ja) | 微生物測定装置とそのために使用される微生物測定用電極チップ、及び微生物測定方法 | |
Strzelczyk et al. | Hydrogel microparticles as sensors for specific adhesion: case studies on antibody detection and soil release polymers | |
Béland et al. | Viability assessment of bacteria using long-range surface plasmon waveguide biosensors | |
Li et al. | Edge-enhanced microwell immunoassay for highly sensitive protein detection | |
Quang Thinh et al. | A label-free electrochemical immunosensor for detection of Newcastle disease virus | |
CN102099494A (zh) | 用于检测和/或定量噬菌体的方法和系统,用于检测所述噬菌体的微电子传感器装置的应用以及用于实现所述方法的微电子传感器装置 | |
Manczak et al. | Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution | |
US20220018832A1 (en) | Materials and Methods for Glycan Profiling | |
CN104237354B (zh) | Ndm-1 dna探针修饰的电极及其制备方法和应用 | |
Zahedipour et al. | Development of flow cytometry-fluorescent in situ hybridization (Flow-FISH) method for detection of PML/RARa Chromosomal Translocation in acute promyelocytic leukemia cell line | |
Ma et al. | Optical imaging of single protein size, charge, mobility, binding and conformational change |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190226 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190226 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190227 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20211111 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20220930 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20221004 |
|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20221101 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20221101 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20221202 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230123 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230118 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230404 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20230602 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230728 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20230905 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20231005 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7369393 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |