JP7291717B2 - 標識せずに白血球の細胞種を決定するためのIn vitro方法 - Google Patents
標識せずに白血球の細胞種を決定するためのIn vitro方法 Download PDFInfo
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Description
第1の領域:Val4=400~650、Val5=200~500、
第2の領域:Val4=600~750、Val5=400~600、
第3の領域:Val4=700~850、Val5=250~400。
Val4=600~900、Val5=300~600。
第1の領域:Val4=400~550、Val5=200~500の領域中の小集団、
第2の領域:Val4=500~700、Val5=200~500の領域中の支配的な主集団、
第3の領域:Val4=700~850、Val5=250~400の領域中の小集団。
第1の領域:Val4=700~850、Val5=300~500の領域中の支配的な主集団、
第2の領域:Val4=550~700、Val5=300~450の領域中の小集団、
第3の領域:Val4=500~700、Val5=450~600の領域中の小集団。
第1の領域:Val4=650~900、Val5=300~500の領域中の支配的な主集団、
第2の領域:Val4=400~600、Val5=300~500の領域中の小集団。
第1の領域:Val4=700~900、Val5=200~400、
第2の領域:Val4=600~750、Val5=400~550、
第3の領域:Val4=500~650、Val5=250~450、
第4の領域:Val4=400~650、Val5=450~700の領域中の小集団。
第1の領域:Val4=235~920、Val5=576~816 (=「骨髄芽球」ゲート(2))、
第2の領域:Val4=235~920、Val5=816~1015 (=「リンパ芽球」ゲート(1))。
第1の領域中の全データ点の>90%、
第2の領域中の全データ点の<10%。
第1の領域中の全データ点の>90%、
第2の領域中の全データ点の<10%。
第1の領域中の全データ点の<90%、
第2の領域中の全データ点の>10%。
第1の領域中の全データ点の<10%、
第2の領域中の全データ点の>90%。
第1の領域中の全データ点の>80%、
第2の領域中の全データ点の<20%。
Claims (17)
- 生物学的サンプルにおいて標識せずに白血球の細胞種を決定するためのin vitro方法であって、顕微鏡観察器具が細胞のイメージングを行い、細胞の物理的パラメータは、自動イメージ分析によって細胞のイメージ表示から確認され、
白血球の細胞種は、物理的パラメータに基づいておよび主成分分析パラメータ(PCAパラメータ)に基づいて決定され、該主成分分析パラメータは、該物理的パラメータのうちの少なくとも一部の一次結合を含み、
該方法は、非染色細胞を使用して実行される、
前記in vitro方法。 - 白血球の決定された細胞種は、以下の白血球の主要な種類、すなわち、単球、好中球、好塩基球、好酸球、およびリンパ球を含む群のうちの1つである、請求項1に記載の方法。
- 決定された細胞種は、骨髄球、後骨髄球、前骨髄球、芽球、巨核球、形質細胞、非定型リンパ球、およびセザリー細胞を含む群からの、白血球の主要な種類ならびに/または白血球の亜型のうちの1つである、請求項1または2に記載の方法。
- サンプル中の多様な白血球のそれぞれの細胞種の決定を使用して、それぞれの細胞集団を特徴づけ、本細胞集団を使用して、サンプルが由来する患者において急性骨髄性白血病(AML)、急性リンパ芽球性白血病(ALL)、慢性骨髄性白血病(CML)、または慢性リンパ球性白血病(CLL)が存在するかどうかを決定する、請求項1~3のいずれか1項に記載の方法。
- 細胞の物理的パラメータは、細胞に覆われた面積、細胞の周囲長、細胞の幅、細胞の高さ、細胞の幅対高さの比、細胞の幾何学的形状の円に対する類似度、細胞の平均半径、細胞の半径の分散、細胞の被覆の度合、細胞の覆われた面積に対応する当量直径、細胞の光学的体積、細胞の最大の光学的高さ、細胞の最小の光学的高さ、細胞の光学的高さの平均
、細胞の光学的高さの分散、細胞の両凹性、細胞の真球度、細胞の質量中心のシフト、細胞のコントラスト、細胞の相違度、細胞の均一性、細胞のエネルギー、細胞のエントロピーを含む群からのパラメータを含む、請求項1~4のいずれか1項に記載の方法。 - 細胞のイメージングは、参照波を対象波上にオーバーレイし、生じるインターフェログラムおよび/またはコンピュータで実行した数学的再構成を記録することを含む、請求項1~5のいずれか1項に記載の方法。
- 顕微鏡観察器具は、デジタルホログラフィー顕微鏡観察(DHM)、干渉位相顕微鏡観察、または定量的位相顕微鏡観察を行うための顕微鏡である、請求項1~6のいずれか1項に記載の方法。
- 白血球は、フローセルにおいて顕微鏡観察器具によってイメージングされる、請求項1~7のいずれか1項に記載の方法。
- フローセルが、長方形または正方形の横断面を有するチャネルを含む、請求項8に記載の方法。
- 白血球は、層状シース流によって集中される、請求項8または9に記載の方法。
- 層状シース流が4つの層状シース流である、請求項10に記載の方法。
- 白血球を顕微鏡観察器具によってイメージングする前に、赤血球を選択的溶解によってサンプルから除去する、請求項1~11のいずれか1項に記載の方法。
- 非染色および非乾燥細胞を使用して実行する、請求項1~12のいずれか1項に記載の方法。
- 以下の工程:
-標識を用いて細胞を表現型決定すること、および/または
-細胞のさらなる割当てのために事前に決定された受容体を発現させること
をさらに含む、請求項1~13のいずれか1項に記載の方法。 - 全血サンプルを使用して行う、ならびに/または、生物学的サンプルは血液細胞、単球、好中球、好塩基球、好酸球、および/もしくはリンパ球を含有する、請求項1~14のいずれか1項に記載の方法。
- 請求項1~15のいずれか1項に記載の方法を実行するように構成されているマイクロチップまたはマイクロコントローラーを含む、細胞分析装置。
- 請求項16に記載の細胞分析装置を含む、生物学的サンプルの細胞の細胞種を標識せずに決定するための顕微鏡観察器具。
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Application Number | Priority Date | Filing Date | Title |
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EP18162033.7A EP3540631A1 (de) | 2018-03-15 | 2018-03-15 | In-vitro-verfahren zum markierungsfreien bestimmen eines zelltyps einer weissen blutzelle |
EP18162033.7 | 2018-03-15 | ||
PCT/EP2019/055965 WO2019175082A1 (de) | 2018-03-15 | 2019-03-11 | In-vitro-verfahren zum markierungsfreien bestimmen eines zelltyps einer weissen blutzelle |
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JP2021518527A JP2021518527A (ja) | 2021-08-02 |
JP7291717B2 true JP7291717B2 (ja) | 2023-06-15 |
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