JP7288457B2 - 正確性が向上したdpo4ポリメラーゼバリアント - Google Patents

正確性が向上したdpo4ポリメラーゼバリアント Download PDF

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JP7288457B2
JP7288457B2 JP2020550060A JP2020550060A JP7288457B2 JP 7288457 B2 JP7288457 B2 JP 7288457B2 JP 2020550060 A JP2020550060 A JP 2020550060A JP 2020550060 A JP2020550060 A JP 2020550060A JP 7288457 B2 JP7288457 B2 JP 7288457B2
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polymerase
polymerases
dna
sequence
amino acid
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JP2021505201A (ja
JP2021505201A5 (enExample
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ココリス マーク
プリンドル マルク
スコット ティエッセン グレゴリー
アイザック レフマン アレクサンダー
グッドマン ドリュー
ジャコブス アアロン
チェイス ジョン
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ストラトス ゲノミクス インコーポレイテッド
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07007DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020550060A 2017-12-11 2018-12-10 正確性が向上したdpo4ポリメラーゼバリアント Active JP7288457B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762597109P 2017-12-11 2017-12-11
US62/597,109 2017-12-11
US201862656696P 2018-04-12 2018-04-12
US62/656,696 2018-04-12
PCT/US2018/064794 WO2019118372A1 (en) 2017-12-11 2018-12-10 Dpo4 polymerase variants with improved accuracy

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JP2021505201A JP2021505201A (ja) 2021-02-18
JP2021505201A5 JP2021505201A5 (enExample) 2022-01-06
JP7288457B2 true JP7288457B2 (ja) 2023-06-07

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US (1) US11530392B2 (enExample)
EP (1) EP3724323A4 (enExample)
JP (1) JP7288457B2 (enExample)
CN (1) CN111655846B (enExample)
AU (1) AU2018383610B2 (enExample)
CA (1) CA3084812A1 (enExample)
WO (1) WO2019118372A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3377648B1 (en) 2015-11-16 2021-01-06 Stratos Genomics, Inc. Dp04 polymerase variants
ES2987024T3 (es) 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy
WO2020236526A1 (en) 2019-05-23 2020-11-26 Stratos Genomics, Inc. Translocation control elements, reporter codes, and further means for translocation control for use in nanopore sequencing
CN114144520B (zh) * 2019-06-26 2024-12-13 豪夫迈·罗氏有限公司 通过芳香族化合物增强核酸聚合
CN120051575A (zh) 2022-10-21 2025-05-27 豪夫迈·罗氏有限公司 对核酸样品中的经修饰的核碱基的检测
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132780A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2016537981A (ja) 2013-11-20 2016-12-08 ノクソン ファーマ エージー 酵素によるl−核酸の合成
CN106318924A (zh) 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
WO2017087281A1 (en) 2015-11-16 2017-05-26 Stratos Genomics, Inc. Dp04 polymerase variants

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US5614617A (en) 1990-07-27 1997-03-25 Isis Pharmaceuticals, Inc. Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
JP3739785B2 (ja) 1991-11-26 2006-01-25 アイシス ファーマシューティカルズ,インコーポレイティド 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形
HU9501963D0 (en) 1993-03-30 1995-08-28 Sterling Winthrop Inc 7-deazapurine modified oligonucleotides
EP0695306A1 (en) 1993-04-19 1996-02-07 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
WO2005045015A2 (en) 2003-11-03 2005-05-19 Medical Research Council Polymerase
WO2005113760A2 (en) 2004-05-20 2005-12-01 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermostable y-family polymerases and chimeras
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
JP5745842B2 (ja) 2007-06-19 2015-07-08 ストラトス ゲノミクス インコーポレイテッド 拡張によるハイスループット核酸配列決定
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US9850471B2 (en) * 2012-05-16 2017-12-26 Noxxon Pharma Ag Enzymatic synthesis of L-nucleic acids
WO2016081871A1 (en) 2014-11-20 2016-05-26 Stratos Genomics, Inc. Nulceoside phosphoroamidate esters and derivatives thereof, use and synthesis thereof
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
ES2987024T3 (es) * 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016537981A (ja) 2013-11-20 2016-12-08 ノクソン ファーマ エージー 酵素によるl−核酸の合成
WO2017087281A1 (en) 2015-11-16 2017-05-26 Stratos Genomics, Inc. Dp04 polymerase variants
CN106318924A (zh) 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶

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US20210163903A1 (en) 2021-06-03
CA3084812A1 (en) 2019-06-20
CN111655846A (zh) 2020-09-11
EP3724323A1 (en) 2020-10-21
JP2021505201A (ja) 2021-02-18
CN111655846B (zh) 2024-05-03
EP3724323A4 (en) 2021-08-18
WO2019118372A1 (en) 2019-06-20
AU2018383610A1 (en) 2020-07-23
US11530392B2 (en) 2022-12-20
AU2018383610B2 (en) 2024-09-12

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