CN111655846B - 具有改进的精确度的dpo4聚合酶变体 - Google Patents

具有改进的精确度的dpo4聚合酶变体 Download PDF

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CN111655846B
CN111655846B CN201880079698.7A CN201880079698A CN111655846B CN 111655846 B CN111655846 B CN 111655846B CN 201880079698 A CN201880079698 A CN 201880079698A CN 111655846 B CN111655846 B CN 111655846B
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CN111655846A (zh
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马克·可可里斯
马克·普林德
格雷戈里·斯科特·蒂森
亚历山大·艾萨克·莱曼
德鲁·古德曼
亚伦·雅各布斯
约翰·查瑟
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201880079698.7A 2017-12-11 2018-12-10 具有改进的精确度的dpo4聚合酶变体 Active CN111655846B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762597109P 2017-12-11 2017-12-11
US62/597,109 2017-12-11
US201862656696P 2018-04-12 2018-04-12
US62/656,696 2018-04-12
PCT/US2018/064794 WO2019118372A1 (en) 2017-12-11 2018-12-10 Dpo4 polymerase variants with improved accuracy

Publications (2)

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CN111655846A CN111655846A (zh) 2020-09-11
CN111655846B true CN111655846B (zh) 2024-05-03

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US (1) US11530392B2 (enExample)
EP (1) EP3724323A4 (enExample)
JP (1) JP7288457B2 (enExample)
CN (1) CN111655846B (enExample)
AU (1) AU2018383610B2 (enExample)
CA (1) CA3084812A1 (enExample)
WO (1) WO2019118372A1 (enExample)

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Publication number Priority date Publication date Assignee Title
EP3377648B1 (en) 2015-11-16 2021-01-06 Stratos Genomics, Inc. Dp04 polymerase variants
ES2987024T3 (es) 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy
WO2020236526A1 (en) 2019-05-23 2020-11-26 Stratos Genomics, Inc. Translocation control elements, reporter codes, and further means for translocation control for use in nanopore sequencing
CN114144520B (zh) * 2019-06-26 2024-12-13 豪夫迈·罗氏有限公司 通过芳香族化合物增强核酸聚合
CN120051575A (zh) 2022-10-21 2025-05-27 豪夫迈·罗氏有限公司 对核酸样品中的经修饰的核碱基的检测
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132780A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads

Citations (4)

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Publication number Priority date Publication date Assignee Title
CN104379748A (zh) * 2012-05-16 2015-02-25 诺松制药股份公司 L-核酸的酶促合成
WO2015074756A1 (en) * 2013-11-20 2015-05-28 Noxxon Pharma Ag Synthesis of l-nucleic acids by means of an enzyme
CN106318924A (zh) * 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
WO2017087281A1 (en) * 2015-11-16 2017-05-26 Stratos Genomics, Inc. Dp04 polymerase variants

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US5614617A (en) 1990-07-27 1997-03-25 Isis Pharmaceuticals, Inc. Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
JP3739785B2 (ja) 1991-11-26 2006-01-25 アイシス ファーマシューティカルズ，インコーポレイティド 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形
HU9501963D0 (en) 1993-03-30 1995-08-28 Sterling Winthrop Inc 7-deazapurine modified oligonucleotides
EP0695306A1 (en) 1993-04-19 1996-02-07 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
WO2005045015A2 (en) 2003-11-03 2005-05-19 Medical Research Council Polymerase
WO2005113760A2 (en) 2004-05-20 2005-12-01 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermostable y-family polymerases and chimeras
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
JP5745842B2 (ja) 2007-06-19 2015-07-08 ストラトス ゲノミクス インコーポレイテッド 拡張によるハイスループット核酸配列決定
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
WO2016081871A1 (en) 2014-11-20 2016-05-26 Stratos Genomics, Inc. Nulceoside phosphoroamidate esters and derivatives thereof, use and synthesis thereof
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
ES2987024T3 (es) * 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104379748A (zh) * 2012-05-16 2015-02-25 诺松制药股份公司 L-核酸的酶促合成
WO2015074756A1 (en) * 2013-11-20 2015-05-28 Noxxon Pharma Ag Synthesis of l-nucleic acids by means of an enzyme
WO2017087281A1 (en) * 2015-11-16 2017-05-26 Stratos Genomics, Inc. Dp04 polymerase variants
CN106318924A (zh) * 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Increased Processivity, Misincorporation, and Nucleotide Incorporation Efficiency in Sulfolobus solfataricus Dpo4 Thumb Domain Mutants;Wang Li等;《Appl Environ Microbiol》;第83卷(第18期);e01013-17 *

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US20210163903A1 (en) 2021-06-03
CA3084812A1 (en) 2019-06-20
CN111655846A (zh) 2020-09-11
JP7288457B2 (ja) 2023-06-07
EP3724323A1 (en) 2020-10-21
JP2021505201A (ja) 2021-02-18
EP3724323A4 (en) 2021-08-18
WO2019118372A1 (en) 2019-06-20
AU2018383610A1 (en) 2020-07-23
US11530392B2 (en) 2022-12-20
AU2018383610B2 (en) 2024-09-12

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