JP7226886B2 - thick tea drink - Google Patents

thick tea drink Download PDF

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JP7226886B2
JP7226886B2 JP2017247738A JP2017247738A JP7226886B2 JP 7226886 B2 JP7226886 B2 JP 7226886B2 JP 2017247738 A JP2017247738 A JP 2017247738A JP 2017247738 A JP2017247738 A JP 2017247738A JP 7226886 B2 JP7226886 B2 JP 7226886B2
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JP2019110839A (en
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由莉佳 矢島
英行 若林
淳平 日置
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Kirin Holdings Co Ltd
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Description

本発明は、濃厚茶飲料に関し、より詳細には、容器詰め濃厚茶飲料に関する。 The present invention relates to concentrated tea beverages, and more particularly to packaged concentrated tea beverages.

近年、消費者の嗜好が多様化し、これに応じた様々な風味や機能性を有する茶飲料が開発されている。濃厚な茶飲料は、茶飲料に含まれる各種成分の濃度が濃く、その香味が強く表れる。例えば、茶飲料に含まれるアミノ酸は飲料に旨味を付与する成分であり、アミノ酸が豊富な濃厚な茶飲料は旨味が強いという特徴を有する。一方で、茶飲料に含まれるポリフェノールは、飲料の苦味や渋味の要因となる成分でもあり、濃厚な茶飲料は苦味や渋みが強くなり、飲用が敬遠される一因ともなりうる。 In recent years, consumer tastes have diversified, and tea beverages having various flavors and functions have been developed accordingly. A rich tea beverage has a high concentration of various components contained in the tea beverage, and its flavor is strongly expressed. For example, amino acids contained in tea beverages are components that impart umami to beverages, and thick tea beverages rich in amino acids are characterized by their strong umami. On the other hand, polyphenols contained in tea beverages are also a factor responsible for the bitterness and astringency of beverages.

これまでにアミノ酸を高濃度で含有する茶飲料の加熱劣化臭を緩和する技術(特許文献1)が知られているが、良好な飲み口を実現した濃厚茶飲料は知られていない。 Until now, a technique for alleviating the heat-deteriorated odor of tea beverages containing amino acids at high concentrations (Patent Document 1) is known, but a thick tea beverage that achieves a good taste is not known.

特開2011-97905号公報JP 2011-97905 A

本発明は、飲み応えがあり、ほどよい苦渋味が付与された濃厚茶飲料を提供することを目的とする。 An object of the present invention is to provide a concentrated tea beverage which is satisfying to drink and imparted with moderate bitterness and astringency.

本発明者らは、濃厚茶飲料において非重合カテキン含量を低減すると飲み応えや苦渋味が不十分になること、非重合カテキン含量が低減された濃厚茶飲料においてカフェイン濃度を所定範囲内に調整することにより、飲み応えがあり、ほどよい苦渋味が付与された濃厚茶飲料を実現できることを見出した。本発明は、これらの知見に基づくものである。 The present inventors have found that reducing the content of non-polymerized catechins in concentrated tea beverages results in insufficient drinking response and bitter and astringent taste, and that the concentration of caffeine in concentrated tea beverages with reduced content of non-polymerized catechins is adjusted within a predetermined range. By doing so, it is possible to realize a thick tea beverage that is satisfying to drink and has a moderate bitterness and astringency. The present invention is based on these findings.

本発明によれば以下の発明が提供される。
[1]下記(a)および(b)を満たす茶飲料:
(a)飲料中の非重合カテキン濃度が、10~80mg/100mLである
(b)飲料中のカフェイン濃度が、40~100mg/100mLである。
[2](c)飲料の粘度が、1.05mPa・s以上である、上記[1]に記載の茶飲料。
[3](d)飲料の可溶性固形分(Brix)が、0.45以上である、上記[1]または[2]に記載の茶飲料。
[4](e)非重合カテキン濃度に対する粘度の比率(以下、「粘度/非重合カテキン濃度比率」ということがある)が、0.013以上である、上記[1]~[3]のいずれかに記載の茶飲料。
[5]緑茶飲料である、上記[1]~[4]のいずれかに記載の茶飲料。
[6]容器詰め飲料である、上記[1]~[5]のいずれかに記載の茶飲料。
According to the present invention, the following inventions are provided.
[1] A tea beverage that satisfies the following (a) and (b):
(a) The concentration of non-polymerized catechin in the beverage is 10-80 mg/100 mL. (b) The concentration of caffeine in the beverage is 40-100 mg/100 mL.
[2] (c) The tea beverage according to [1] above, wherein the beverage has a viscosity of 1.05 mPa·s or more.
[3] (d) The tea beverage according to [1] or [2] above, wherein the soluble solids content (Brix) of the beverage is 0.45 or more.
[4] (e) Any of the above [1] to [3], wherein the ratio of viscosity to non-polymerized catechin concentration (hereinafter sometimes referred to as "viscosity/non-polymerized catechin concentration ratio") is 0.013 or more The tea beverage described in Crab.
[5] The tea beverage according to any one of [1] to [4] above, which is a green tea beverage.
[6] The tea beverage according to any one of [1] to [5] above, which is a packaged beverage.

本発明によれば、飲み応えがあり、ほどよい苦渋味が付与された濃厚茶飲料が提供される。本発明の茶飲料は、濃厚茶飲料でありながら、良好な飲み口を実現するものであり、消費者の多様な嗜好ニーズを満たす点で有利である。 ADVANTAGE OF THE INVENTION According to this invention, the thick tea drink which is satisfying to drink, and was given moderate bitterness and astringency is provided. INDUSTRIAL APPLICABILITY The tea beverage of the present invention is a thick tea beverage, yet has a good taste, and is advantageous in that it satisfies various taste needs of consumers.

発明の具体的説明Specific description of the invention

本発明の茶飲料は、茶飲料の成分が濃い茶飲料、すなわち、濃厚な茶飲料である。本発明において茶飲料の濃厚さの程度は、飲料の粘度および可溶性固形分(Brix)を指標にして決定することができる。 The tea beverage of the present invention is a tea beverage with strong tea beverage ingredients, that is, a concentrated tea beverage. In the present invention, the degree of richness of the tea beverage can be determined using the viscosity and soluble solids content (Brix) of the beverage as indices.

本発明の茶飲料の粘度の下限値(以上または超)は、1.05mPa・sとすることができ、好ましくは1.07mPa・s、より好ましくは1.08mPa・sである。本発明の茶飲料の粘度の上限値(以下または未満)は、特に限定されるものではないが、例えば、1.30mPa・sとすることができ、好ましくは1.25mPa・sである。これらの下限値および上限値はそれぞれ任意に組み合わせることができる。茶飲料の粘度は、市販の落球式マイクロ粘度計を使用して測定することができる。 The lower limit (or more or more) of the viscosity of the tea beverage of the present invention can be 1.05 mPa·s, preferably 1.07 mPa·s, more preferably 1.08 mPa·s. The upper limit value (below or less than) of the viscosity of the tea beverage of the present invention is not particularly limited, but can be, for example, 1.30 mPa·s, preferably 1.25 mPa·s. These lower and upper limits can be combined arbitrarily. The viscosity of tea beverages can be measured using a commercially available falling ball microviscometer.

本発明の茶飲料の可溶性固形分(Brix)の下限値(以上または超)は、0.45とすることができ、好ましくは0.60である。本発明の茶飲料の可溶性固形分の上限値(以下または未満)は、特に限定されるものではないが、例えば、1.8とすることができ、好ましくは1.57である。これらの下限値および上限値はそれぞれ任意に組み合わせることができる。茶飲料の可溶性固形分は、市販の屈折糖度計を使用して測定することができる。 The lower limit (or more or more) of the soluble solids content (Brix) of the tea beverage of the present invention can be 0.45, preferably 0.60. The upper limit of the soluble solids content (below or less than) of the tea beverage of the present invention is not particularly limited, but can be, for example, 1.8, preferably 1.57. These lower and upper limits can be combined arbitrarily. The soluble solids content of tea beverages can be measured using a commercially available refractometer.

本発明の茶飲料は、飲料中の非重合カテキン濃度が所定値であることを特徴とする。飲料中の非重合カテキン濃度の上限値(以下または未満)は、80mg/100mLである。飲料中の非重合カテキン濃度の下限値(以上または超)は、10mg/100mLであり、好ましくは40mg/100mLであり、さらに好ましくは60mg/100mLである。これらの上限値および下限値はそれぞれ任意に組み合わせることができる。茶飲料の非重合カテキン濃度は、カテキン、ガロカテキン、カテキンガレート、ガロカテキンガレート、エピカテキン、エピガロカテキン、エピカテキンガレートおよびエピガロカテキンガレートのそれぞれの濃度の合計量であり、高速液体クロマトグラフィー(HPLC)法により測定することができる。 The tea beverage of the present invention is characterized in that the non-polymerized catechin concentration in the beverage is a predetermined value. The upper limit of non-polymerized catechin concentration in beverages (below or below) is 80 mg/100 mL. The lower limit (above or above) of the non-polymerized catechin concentration in the beverage is 10 mg/100 mL, preferably 40 mg/100 mL, more preferably 60 mg/100 mL. These upper and lower limits can be combined arbitrarily. The concentration of non-polymerized catechins in tea beverages is the sum of the respective concentrations of catechin, gallocatechin, catechin gallate, gallocatechin gallate, epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin gallate, and is determined by high-performance liquid chromatography ( HPLC) method.

本発明の茶飲料は、飲料中のカフェイン濃度が所定値であることを特徴とする。飲料中のカフェイン濃度の上限値(以下または未満)は、100mg/100mLである。飲料中のカフェイン濃度の下限値(以上または超)は、40mg/100mLであり、好ましくは60mg/100mLであり、さらに好ましくは80mg/100mLである。これらの上限値および下限値はそれぞれ任意に組み合わせることができる。茶飲料のカフェイン濃度は、HPLC法により測定することができる。 The tea beverage of the present invention is characterized in that the caffeine concentration in the beverage is a predetermined value. The upper limit for caffeine concentration in beverages (below or below) is 100 mg/100 mL. The lower limit (above or above) of caffeine concentration in the beverage is 40 mg/100 mL, preferably 60 mg/100 mL, more preferably 80 mg/100 mL. These upper and lower limits can be combined arbitrarily. The caffeine concentration of tea beverages can be measured by the HPLC method.

本発明の茶飲料は、非重合カテキン濃度に対する粘度の比率により特定することもできる。非重合カテキン濃度は茶飲料の苦渋味成分の指標であり、粘度は茶飲料の旨み成分の指標でもあるため、この比率は、本発明の茶飲料における旨み成分の比率を示しているといえる。本発明の飲料の粘度/非重合カテキン濃度比率の下限値(以上または超)は、0.013とすることができる。本発明の飲料の粘度/非重合カテキン濃度比率の上限値(以下または未満)は、0.13とすることができる。 The tea beverages of the present invention can also be identified by the ratio of viscosity to non-polymerized catechin concentration. Since the non-polymerized catechin concentration is an index of the bitterness and astringency components of the tea beverage, and the viscosity is also an index of the umami components of the tea beverage, it can be said that this ratio indicates the ratio of the umami components in the tea beverage of the present invention. The lower limit (or more or more) of the viscosity/non-polymerized catechin concentration ratio of the beverage of the present invention can be 0.013. The upper limit (below or less than) of the viscosity/unpolymerized catechin concentration ratio of the beverage of the present invention can be 0.13.

本発明の茶飲料としては、特に限定されないが、本発明に適用可能な茶飲料としては、例えば、不発酵茶(例えば、緑茶、ルイボス茶)飲料、発酵茶(例えば、紅茶)飲料、半発酵茶(例えば、烏龍茶)飲料、後発酵茶(例えば、プーアル茶)飲料および穀物茶(例えば、麦茶)飲料並びにこれらの一部または全部のブレンド茶飲料が挙げられる。 The tea beverage of the present invention is not particularly limited, but examples of tea beverages applicable to the present invention include non-fermented tea (e.g., green tea, rooibos tea) beverages, fermented tea (e.g., black tea) beverages, and semi-fermented tea beverages. Tea (eg, oolong tea) beverages, post-fermented tea (eg, pu-erh tea) beverages and grain tea (eg, barley tea) beverages, and blended tea beverages of some or all of these are included.

本発明の茶飲料は、通常の茶抽出液の調製に用いられている方法を用いて製造される茶抽出液やその濃縮液または希釈液から調製することができる。例えば、茶葉と水(0~100℃)を混合接触させるか、あるいは、茶エキスや茶パウダーなどの茶抽出液の濃縮物や精製物を水(0~100℃)に混合または溶解させることにより、本発明に用いられる茶抽出液を得ることができる。また、上記の茶葉と水を混合接触させて得られる茶抽出液と、上記の茶エキスや茶パウダーとを混合したものを茶抽出液として本発明の茶飲料に用いてもよい。茶葉と水を混合接触させた場合には、遠心分離や濾過などの分離手段を用いて茶葉と茶抽出液を分離することができる。また、茶抽出液の調製に際し茶葉以外の任意の原料を配合してよい。 The tea beverage of the present invention can be prepared from a tea extract produced using a method commonly used for preparing tea extracts, or a concentrate or dilution thereof. For example, by mixing and contacting tea leaves and water (0 to 100 ° C.), or by mixing or dissolving a concentrate or purified product of tea extract such as tea extract or tea powder in water (0 to 100 ° C.) , the tea extract used in the present invention can be obtained. A mixture of the tea extract obtained by mixing and contacting the above tea leaves and water with the above tea extract or tea powder may be used as the tea extract in the tea beverage of the present invention. When the tea leaves and water are brought into contact with each other, separation means such as centrifugation or filtration can be used to separate the tea leaves and the tea extract. Any raw materials other than tea leaves may be blended in preparation of the tea extract.

茶抽出液の調製に用いられる原料は、特に限定されないが、穀物など(麦、米など)やマメ科植物(ルイボスなど)の他、ツバキ科の常緑樹である茶樹カメリア・シネンシス(Camellia sinensis var.)に属する茶葉を用いることができる。本発明の茶飲料の製造に用いられる茶葉は、本発明の効果を奏する限り特に限定されるものではなく、煎茶、玉露、抹茶、釜炒り茶、番茶、ほうじ茶などの緑茶に代表される不発酵茶の茶葉に限らず、烏龍茶のような半発酵茶の茶葉や、紅茶のような発酵茶の茶葉、プーアル茶のような後発酵茶の茶葉なども用いることができる。本発明に用いられる茶葉としては、好ましくは緑茶葉である。本発明においてはまた、複数種類の原料および茶葉が使用されてもよい。なお、本発明において「緑茶」は、緑茶にマツリカ(茉莉花)の花の香りを着香させたジャスミン茶を包含する。 The raw materials used for the preparation of the tea extract are not particularly limited, but include grains (wheat, rice, etc.), leguminous plants (rooibos, etc.), and evergreen tea tree Camellia sinensis var. ) can be used. The tea leaves used in the production of the tea beverage of the present invention are not particularly limited as long as the effects of the present invention are achieved, and are represented by green tea such as sencha, gyokuro, matcha, kamairicha, bancha, and hojicha. Not only tea leaves but also semi-fermented tea leaves such as oolong tea, fermented tea leaves such as black tea, and post-fermented tea leaves such as pu-erh tea can be used. The tea leaves used in the present invention are preferably green tea leaves. Multiple types of ingredients and tea leaves may also be used in the present invention. In the present invention, "green tea" includes jasmine tea, which is green tea flavored with the fragrance of matsurika (matsurika) flowers.

本発明において「茶抽出液」とは、茶葉を抽出処理に付することにより得られる、抽出液を意味する。本発明に用いられる茶抽出液は、茶葉からの抽出液それ自体や、その加工品類(例えば、濃縮液体エキス、粉末エキス)などが挙げられ、従来、茶飲料の製造に用いられている茶抽出原料であれば、特に限定されず、適宜選択することができる。 In the present invention, "tea extract" means an extract obtained by subjecting tea leaves to extraction treatment. The tea extract used in the present invention includes the extract itself from tea leaves, its processed products (e.g., concentrated liquid extract, powder extract), etc. Conventionally, tea extracts used in the production of tea beverages The raw material is not particularly limited and can be selected as appropriate.

茶葉の抽出処理は、特に限定されず、食品加工分野で一般的に用いられている種々の抽出方法を用いることができ、例えば、溶媒抽出、気流抽出、圧搾抽出などが包含され、必要に応じて、沈殿もしくは濾過などの固液分離、濃縮、遠心分離、乾燥(例えば、噴霧乾燥、凍結乾燥)または粉末化などの処理をさらに施してもよい。 The extraction treatment of tea leaves is not particularly limited, and various extraction methods commonly used in the food processing field can be used. Further processing such as solid-liquid separation such as precipitation or filtration, concentration, centrifugation, drying (eg, spray drying, freeze drying) or pulverization may be performed.

ここで、溶媒抽出で用いられる抽出溶媒としては、水(例えば、硬水、軟水、イオン交換水および天然水)が望ましい。抽出溶媒の量は、当業者が適宜選択することができ、特に限定されない。例えば、抽出溶媒が水の場合は、その量は、茶葉の1~100倍量(質量)である。 Here, water (for example, hard water, soft water, ion-exchanged water, and natural water) is desirable as the extraction solvent used in the solvent extraction. The amount of extraction solvent can be appropriately selected by those skilled in the art and is not particularly limited. For example, when the extraction solvent is water, the amount is 1 to 100 times the amount (mass) of tea leaves.

抽出温度および時間は、当業者が適宜選択することができ、特に限定されない。例えば、抽出溶媒が水の場合は、その温度および時間は、10~120℃で1分~12時間である。 The extraction temperature and time can be appropriately selected by those skilled in the art and are not particularly limited. For example, when the extraction solvent is water, the temperature and time are 10 to 120° C. for 1 minute to 12 hours.

抽出処理の一例としては、茶葉を、水中に、0~90℃で、1分~24時間浸漬および攪拌し、その後、茶葉を濾過または遠心分離する方法が挙げられる。ここで、抽出時の温度や時間などの条件は、特に限定されず、茶葉の種類や量によって当業者が任意に選択し、かつ設定することができる。 An example of the extraction treatment includes immersing and stirring tea leaves in water at 0-90° C. for 1 minute to 24 hours, and then filtering or centrifuging the tea leaves. Here, conditions such as temperature and time for extraction are not particularly limited, and can be arbitrarily selected and set by those skilled in the art according to the type and amount of tea leaves.

茶抽出液の調製において、茶エキスや茶パウダーなどの茶抽出液の濃縮物や精製物を用いてもよく、例えば、Value Instant Tea(Kroger社製)やポリフェノン(三井農林社製)、サンフェノン(太陽化学社製)、テアフラン(伊藤園社製)などの市販品を用いることができる。また、これらの茶濃縮物や茶精製物は、そのまままたは水で溶解もしくは希釈したものを単独で使用しても、複数の種類を混合して用いても、茶抽出液と混合して用いてもよい。 In the preparation of the tea extract, a concentrate or purified tea extract such as tea extract or tea powder may be used. Commercially available products such as Taiyo Kagaku Co., Ltd.) and Theafuran (Itoen Co., Ltd.) can be used. In addition, these tea concentrates and purified tea products may be used alone or after being dissolved or diluted with water, may be used as a mixture of a plurality of types, or may be used after being mixed with a tea extract. good too.

本発明の茶飲料では、通常の飲料の製造に用いられている飲料用添加剤、例えば、酸味料、香料、色素、果汁、甘味料(高甘味度甘味料を含む)、食品添加剤(例えば、起泡・泡持ち向上剤、苦味料、保存料、酸化防止剤、増粘安定剤、乳化剤、食物繊維、pH調整剤)などを添加してもよい。 In the tea beverage of the present invention, beverage additives that are commonly used in the production of beverages, such as acidulants, flavors, pigments, fruit juices, sweeteners (including high-intensity sweeteners), food additives (e.g. , foaming/foam retention improver, bittering agent, preservative, antioxidant, thickening stabilizer, emulsifier, dietary fiber, pH adjuster) and the like may be added.

本発明の茶飲料は、容器詰め飲料の形態で提供することができる。容器詰め飲料の容器とは、内容物と外気との接触を断つことができる密閉容器を意味し、例えば、金属缶、樽容器、プラスチック製ボトル(例えば、PETボトル、カップ)、紙容器、瓶、パウチ容器などが挙げられる。 The tea beverage of the present invention can be provided in the form of a packaged beverage. Containers for packaged beverages refer to closed containers that can cut off contact between the contents and the outside air, such as metal cans, barrels, plastic bottles (e.g., PET bottles, cups), paper containers, and bottles. , pouch containers and the like.

本発明の茶飲料の製造は、飲料の粘度、可溶性固形分、非重合カテキン濃度およびカフェイン濃度が所定値となるように調製する以外は、飲料の製造に用いられる通常の方法に従って実施することができる。茶飲料の通常の調製方法は公知であり、例えば、茶抽出液を準備し、調合工程を経て茶飲料を製造することができる。本発明の飲料の製造においては、通常の飲料の処方設計に用いられている飲料用添加剤を添加してもよく、これら添加剤の添加時期は特に制限されない。 The production of the tea beverage of the present invention should be carried out according to a normal method used for producing beverages, except that the viscosity, soluble solids content, non-polymerized catechin concentration, and caffeine concentration of the beverage are adjusted to predetermined values. can be done. A typical method for preparing tea beverages is known, for example, a tea extract can be prepared and a tea beverage can be produced through a blending process. In the production of the beverage of the present invention, beverage additives that are commonly used in formulating and designing beverages may be added, and the timing of addition of these additives is not particularly limited.

ここで、本発明の茶飲料は濃厚な茶飲料であることを特徴とするが、茶抽出液の調製の際に低倍率で抽出することや、茶抽出液の希釈率を緩和することにより、飲料の粘度および飲料の可溶性固形分(Brix)を所定範囲に調整することができる。また、本発明の茶飲料は、濃厚でありながら、非重合カテキン濃度が低減されていることを特徴とするが、茶抽出液をポリビニルポリピロリドン(PVPP)などの非重合カテキンの吸着剤と接触させることにより、茶飲料の非重合カテキン濃度を低減し、所定値とすることができる(例えば、特開平9-220055号公報参照)。さらに、本発明の茶飲料は、カフェイン濃度が所定範囲であることを特徴とするが、茶葉抽出液の希釈、カフェインの除去あるいはカフェインの添加により飲料中のカフェイン濃度を所定値とすることができる。 Here, the tea beverage of the present invention is characterized by being a concentrated tea beverage. The viscosity of the beverage and the soluble solids content (Brix) of the beverage can be adjusted within a predetermined range. In addition, the tea beverage of the present invention is characterized in that the concentration of non-polymerized catechins is reduced while being rich. By increasing the concentration, the concentration of non-polymerized catechin in the tea beverage can be reduced to a predetermined value (see, for example, Japanese Patent Application Laid-Open No. 9-220055). Furthermore, the tea beverage of the present invention is characterized by having a caffeine concentration within a predetermined range. can do.

本発明の茶飲料の製造に当たっては、当業界に公知の製造技術を用いて製造することができ、例えば、「改訂新版ソフトドリンクス」(株式会社光琳)を参考とすることができる。 The tea beverage of the present invention can be produced using production techniques known in the art, for example, "Revised New Edition Soft Drinks" (Kourin Co., Ltd.) can be referred to.

本発明により提供される茶飲料は、抽出工程、調合工程、充填工程および殺菌工程などの工程を経て容器詰め飲料として提供することができる。例えば、調合工程で得られた茶飲料を常法に従って殺菌し、容器に充填することができる。殺菌は容器への充填前であっても充填後であってもよい。 The tea beverage provided by the present invention can be provided as a packaged beverage through processes such as an extraction process, a preparation process, a filling process and a sterilization process. For example, the tea beverage obtained in the preparation process can be sterilized by a conventional method and filled into a container. Sterilization may be before or after filling the container.

本発明の茶飲料の製造において殺菌処理を行う場合、食品分野で一般的に用いられている種々の殺菌方法を用いることができ、典型的には加熱殺菌法を用いることができる。使用できる殺菌方法としては、例えば、レトルト殺菌、UHT殺菌、低温殺菌、HTST殺菌などが挙げられる。これらの加熱殺菌方法は飲料の製造に通常用いられている条件で実施することができる。 When sterilization is performed in the production of the tea beverage of the present invention, various sterilization methods commonly used in the food field can be used, typically heat sterilization can be used. Sterilization methods that can be used include, for example, retort sterilization, UHT sterilization, pasteurization, HTST sterilization, and the like. These heat sterilization methods can be carried out under conditions normally used for the production of beverages.

以下の例に基づき本発明をより具体的に説明するが、本発明はこれらの例に限定されるものではない。 The present invention will be described more specifically based on the following examples, but the present invention is not limited to these examples.

例1:緑茶飲料の調製と官能評価
(1)サンプル飲料の調製
緑茶葉と、緑茶葉の乾燥質量に対して10倍質量の70℃の水を抽出溶媒として用いて6分間抽出した後、茶葉を分離し、抽出液を20℃に冷却した。次いで、抽出液に対して0質量%、1質量%、2質量%または4質量%のポリビニルポリピロリドン(アイエスピージャパン社製)を添加して15分間接触させた後、3000rpm、5分間の遠心分離を行いポリビニルピロリドンや粗大な粉砕茶組織、茶粒子などの固形分を除去した後、濾過により残存した固形物を完全に除き、抽出液A(非重合カテキン濃度:494mg/100mL、カフェイン濃度:169mg/100mL)、抽出液B(非重合カテキン濃度:135mg/100mL、カフェイン濃度:138mg/100mL)、抽出液C(非重合カテキン濃度:35mg/100mL、カフェイン濃度:156mg/100mL)および抽出液D(非重合カテキン濃度:6mg/100mL、カフェイン濃度:146mg/100mL)を得た。次いで、これらの抽出液を任意の割合で希釈および/または混合し、場合によっては非重合カテキン製剤(ポリフェノン70A、三井農林社製)を添加することにより、表3に示す所定のカフェイン濃度および所定のカテキン濃度の緑茶サンプル飲料(サンプル番号1~30)を調製した。
Example 1: Preparation of green tea beverage and sensory evaluation (1) Preparation of sample beverage Green tea leaves and water at 70 ° C. that is 10 times the weight of the dry weight of the green tea leaves are used as an extraction solvent for 6 minutes. was separated and the extract was cooled to 20°C. Next, 0% by mass, 1% by mass, 2% by mass or 4% by mass of polyvinylpolypyrrolidone (manufactured by ISP Japan) is added to the extract and contacted for 15 minutes, followed by centrifugation at 3000 rpm for 5 minutes. After separating and removing solids such as polyvinylpyrrolidone, coarse ground tea tissue, and tea particles, the remaining solids are completely removed by filtration, and extract liquid A (non-polymerized catechin concentration: 494 mg / 100 mL, caffeine concentration : 169 mg / 100 mL), extract B (non-polymerized catechin concentration: 135 mg / 100 mL, caffeine concentration: 138 mg / 100 mL), extract C (non-polymerized catechin concentration: 35 mg / 100 mL, caffeine concentration: 156 mg / 100 mL) and An extract D (non-polymerized catechin concentration: 6 mg/100 mL, caffeine concentration: 146 mg/100 mL) was obtained. Next, these extracts are diluted and / or mixed at an arbitrary ratio, and in some cases, a non-polymerized catechin preparation (Polyphenon 70A, manufactured by Mitsui Norin Co., Ltd.) is added to obtain a predetermined caffeine concentration and Green tea sample beverages (sample numbers 1 to 30) with predetermined catechin concentrations were prepared.

(2)サンプル飲料の分析 (2) Analysis of sample beverages

上記(1)で調製した飲料のカフェイン濃度(mg/100mL)は、HPLC法により測定した。具体的には、試料溶液を表1に示すHPLC分析条件で高速液体クロマトグラフィー(HPLC)に供し、カフェイン濃度を測定した。 The caffeine concentration (mg/100 mL) of the beverage prepared in (1) above was measured by the HPLC method. Specifically, the sample solution was subjected to high performance liquid chromatography (HPLC) under the HPLC analysis conditions shown in Table 1 to measure the caffeine concentration.

Figure 0007226886000001
Figure 0007226886000001

上記(1)で調製した飲料の非重合カテキン濃度(mg/100mL)は、HPLC法により測定した。具体的には、試料溶液を表2に示すHPLC分析条件で高速液体クロマトグラフィー(HPLC)に供し、非重合カテキン濃度を測定した。 The non-polymerized catechin concentration (mg/100 mL) of the beverage prepared in (1) above was measured by the HPLC method. Specifically, the sample solution was subjected to high performance liquid chromatography (HPLC) under the HPLC analysis conditions shown in Table 2 to measure the non-polymerized catechin concentration.

Figure 0007226886000002
Figure 0007226886000002

上記(1)で調製した飲料の可溶性固形分(Brix)は、屈折糖度計(RX-5000α、アタゴ(ATAGO)社製)を用いて測定した。上記(1)で調製した飲料の粘度(mPa・s)は、落球式マイクロ粘度計(アントンパール社製)を用いて測定した。また、上記で得られた粘度の値を用いて、非重合カテキン濃度に対する粘度の比率(粘度(mPa・s)/非重合カテキン濃度(mg/100mL))を算出した。さらに、上記(1)で調製した飲料を5℃で2日間静置保管し、分光光度計(U‐3900H、日立ハイテクサイエンス社製)を用いてOD660の吸光度を測定し、濁度を評価した。 The soluble solid content (Brix) of the beverage prepared in (1) above was measured using a refractometer (RX-5000α, manufactured by ATAGO). The viscosity (mPa·s) of the beverage prepared in (1) above was measured using a falling ball micro viscometer (manufactured by Anton Paar). Using the viscosity values obtained above, the ratio of viscosity to non-polymerized catechin concentration (viscosity (mPa·s)/non-polymerized catechin concentration (mg/100 mL)) was calculated. Furthermore, the beverage prepared in (1) above was stored at 5 ° C. for 2 days, and the absorbance of OD660 was measured using a spectrophotometer (U-3900H, manufactured by Hitachi High-Tech Science) to evaluate the turbidity. .

(3)官能評価
上記(1)で得られた飲料を官能評価に供した。評価項目は、「飲み応え」と「苦渋味」の2項目とした。ここで、「飲み応え」とは濃い茶感を口、喉で感じる食感をいい、「苦渋味」とは舌で感じる収斂する香味をいう。官能評価は訓練されたパネラー4名により以下の手順で実施した。飲み応えについて、サンプル飲料11を基準品として評点を2点と固定して官能評価を行い、香味と評点をパネラー間で共通の認識としたうえで、他のサンプル飲料について官能評価を行った。また、苦渋味について、サンプル飲料11を基準品として評点を1点と固定して官能評価を行い、香味と評点をパネラー間で共通の認識としたうえで、他のサンプル飲料について官能評価を行った。各評価項目のパネラーの評点の平均値を算出した。評価基準は以下に示した通りである。
(3) Sensory Evaluation The beverage obtained in (1) above was subjected to sensory evaluation. There were two evaluation items: "Drinkability" and "Bitter and astringent taste". Here, the term "drinkability" refers to the texture of a strong tea feeling felt in the mouth and throat, and the term "bitter and astringent taste" refers to an astringent flavor felt on the tongue. The sensory evaluation was carried out by four trained panelists according to the following procedure. Regarding the response to drinking, a sensory evaluation was performed with the sample beverage 11 as a reference product and a fixed score of 2 points. After sharing the flavor and score among the panelists, sensory evaluation was performed on the other sample beverages. In addition, regarding the bitterness and astringency, a sensory evaluation was performed with the sample beverage 11 as a reference product and the score was fixed at 1 point. After the flavor and the score were shared among the panelists, sensory evaluation was performed on the other sample beverages. rice field. The average score of the panelists for each evaluation item was calculated. The evaluation criteria are as shown below.

<飲み応え>
5点:基準品に比べとても強く感じられる
4点:基準品に比べ強く感じられる
3点:基準品に比べやや強く感じられる
2点:基準品と同等で、飲み応えをやや感じる
1点:基準品に比べ弱く感じられる
<苦渋味>
4点:基準品に比べとても強く感じられる
3点:基準品に比べ強く感じられる
2点:基準品に比べやや強く感じられちょうどよい
1点:基準品と同等で、苦渋味が弱い
<Drinking response>
5 points: Feels very strong compared to the standard product 4 points: Feels stronger than the standard product 3 points: Feels slightly stronger than the standard product 2 points: Equivalent to the standard product and feels a little more filling 1 point: Standard <Bitterness and astringency>
4 points: Feels very strong compared to the standard product 3 points: Feels strong compared to the standard product 2 points: Feels slightly stronger than the standard product and is just right 1 point: Same as the standard product, weak bitterness and astringency

飲み応えの評価で得られた評点に基づいて、平均値が4.5以上のものを「A」、平均値が3.5以上4.5未満のものを「B」、平均値が2.5以上3.5未満のものを「C」、平均値が1.5以上2.5未満のものを「D」、平均値が1.5未満のものを「E」と評価した。また、苦渋味の評価で得られた評点に基づいて、平均値が3.5以上のものを「++」、平均値が2.5以上3.5未満のものを「+」、平均値が1.5以上2.5未満のものを「◎」、平均値が1.5未満のものを「-」と評価した。 Based on the scores obtained in the evaluation of the response to drinking, "A" indicates an average value of 4.5 or more, "B" indicates an average value of 3.5 or more and less than 4.5, and the average value is 2.5. Those with an average value of 5 or more and less than 3.5 were evaluated as "C", those with an average value of 1.5 or more and less than 2.5 were evaluated as "D", and those with an average value of less than 1.5 were evaluated as "E". In addition, based on the scores obtained in the evaluation of bitterness and astringency, "++" indicates an average value of 3.5 or more, "+" indicates an average value of 2.5 or more and less than 3.5, and "+" indicates an average value of 3.5 or more. Those with an average value of 1.5 or more and less than 2.5 were evaluated as "⊚", and those with an average value of less than 1.5 were evaluated as "-".

(4)結果
上記(1)で得られたサンプル飲料の分析値を表3に示す。

Figure 0007226886000003
(4) Results Table 3 shows the analysis values of the sample beverages obtained in (1) above.
Figure 0007226886000003

官能評価結果を表4および表5に示す。

Figure 0007226886000004
Sensory evaluation results are shown in Tables 4 and 5.
Figure 0007226886000004

Figure 0007226886000005
Figure 0007226886000005

表4および表5の結果から、所定の粘度およびBrixを満たす濃厚な緑茶飲料において、非重合カテキン濃度を所定範囲内に調整し、カフェイン濃度を所定範囲内に調整することで、飲み応えがあり、ほどよい苦渋味が付与された緑茶飲料を実現できることが確認された。また、飲料中の非重合カテキン濃度が、10~80mg/100mLであり、かつ、飲料中のカフェイン濃度が、40~100mg/100mLであるサンプル飲料は、5℃で2日間静置保管後の吸光度(OD660)が0.2以下であり、容器詰め茶飲料として遜色のない濁度であることが確認された。 From the results of Tables 4 and 5, in a thick green tea beverage that satisfies a predetermined viscosity and Brix, by adjusting the non-polymerized catechin concentration within a predetermined range and adjusting the caffeine concentration within a predetermined range, the drinkability is improved. It was confirmed that a green tea beverage imparted with moderate bitterness and astringency can be realized. In addition, a sample beverage having a non-polymerized catechin concentration of 10 to 80 mg / 100 mL and a caffeine concentration of 40 to 100 mg / 100 mL was stored at 5 ° C. for 2 days. It was confirmed that the absorbance (OD660) was 0.2 or less, and the turbidity was comparable to a packaged tea beverage.

Claims (3)

下記(a)、(b)および(d)を満たす容器詰め緑茶飲料:
(a)飲料中の非重合カテキン濃度が、10~80mg/100mLである
(b)飲料中のカフェイン濃度が、40~100mg/100mLであ
(d)飲料の可溶性固形分(Brix)が、0.45以上である。
A packaged green tea beverage that satisfies (a), (b) and (d) below:
(a) the non-polymerized catechin concentration in the beverage is 10-80 mg/100 mL (b) the caffeine concentration in the beverage is 40-100 mg/100 mL
(d) The soluble solids content (Brix) of the beverage is 0.45 or more.
(c)飲料の粘度が、1.05mPa・s以上である、請求項1に記載の緑茶飲料。 (c) The green tea beverage according to claim 1, wherein the beverage has a viscosity of 1.05 mPa·s or more. (e)非重合カテキン濃度に対する粘度の比率(粘度/非重合カテキン濃度比率)が、0.013以上である、請求項1または2に記載の緑茶飲料。 3. The green tea beverage according to claim 1, wherein (e) the ratio of viscosity to non-polymerized catechin concentration (viscosity/non-polymerized catechin concentration ratio) is 0.013 or more.
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