JP7193075B2 - Nukadoko material, its manufacturing method, and nukadoko - Google Patents

Nukadoko material, its manufacturing method, and nukadoko Download PDF

Info

Publication number
JP7193075B2
JP7193075B2 JP2018112473A JP2018112473A JP7193075B2 JP 7193075 B2 JP7193075 B2 JP 7193075B2 JP 2018112473 A JP2018112473 A JP 2018112473A JP 2018112473 A JP2018112473 A JP 2018112473A JP 7193075 B2 JP7193075 B2 JP 7193075B2
Authority
JP
Japan
Prior art keywords
rice bran
producing
lactic acid
bed material
bed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018112473A
Other languages
Japanese (ja)
Other versions
JP2019213487A (en
Inventor
三郎 松井
汐見修一
Original Assignee
株式会社松井三郎環境設計事務所
株式会社Sky・ライフ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社松井三郎環境設計事務所, 株式会社Sky・ライフ filed Critical 株式会社松井三郎環境設計事務所
Priority to JP2018112473A priority Critical patent/JP7193075B2/en
Publication of JP2019213487A publication Critical patent/JP2019213487A/en
Application granted granted Critical
Publication of JP7193075B2 publication Critical patent/JP7193075B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Storage Of Fruits Or Vegetables (AREA)

Description

本発明は、ぬか床材料およびその製造方法並びにぬか床に関し、更に詳細には、米ぬかを主原料とし、漬ける野菜・果物に塩分が無添加であることが実行で来る、2℃~15℃の温度条件下で使用されるぬか床のためのぬか床材料、およびその製造方法に関する。 The present invention relates to a bran bed material, a method for producing the same, and a bran bed. The present invention relates to a bran bed material for bran beds used under temperature conditions, and a method for producing the same.

伝統的な野菜の漬物は、米糠、酒粕、麹等の醗酵物を漬物床として漬込む場合が多い。 Traditional vegetable pickles are often pickled with fermented products such as rice bran, sake lees, and koji as a base for pickles.

これらの漬物床のうち、酒粕、麹は、高価なために利用され難くいので、米糠が最も多く原料として使用されている。しかし、米糠を醗酵させた漬物床である「糠床」は、カビが発生し、水分が浮き易いので毎日掻き混ぜる必要がある。このように従来のぬか床は、手入れが煩瑣であり、それを怠ると腐敗して耐え難い異臭を発生する。従って、近年の様に気密性が高い家屋内に於いてはなかなか利用し難いものであり、限られた野菜(ナス、キュウリ、白菜、大根、その他)しか利用できない。 Of these pickles, sake lees and koji are difficult to use because they are expensive, so rice bran is the most commonly used raw material. However, ``Nukadoko'', which is a pickle bed made by fermenting rice bran, tends to mold and float easily, so it needs to be stirred every day. Thus, conventional rice bran beds are troublesome to maintain, and if left unattended, they will rot and emit an intolerable offensive odor. Therefore, it is difficult to use it in a highly airtight house like in recent years, and only limited vegetables (eggplant, cucumber, Chinese cabbage, radish, etc.) can be used.

近年、米糠や“ふすま”の代わりに、豆腐、豆乳等の製造に際して副次的に大量に生産される“おから”を用いた漬物床が提案されている。また、豆類、穀類又はイモ類の利用残渣に、乳酸菌(ラクトバチルス プランタルムLactobacillus plantarum)を添加し、低温で醗酵させた漬物床が提案されている。しかし、公知の乳酸菌により熟成させる漬物床は、生菌体によるCOやエチレンガスの発生による弊害をもたらすガスが発生するので、市場性が極めて限られている。 In recent years, instead of rice bran and bran, there has been proposed a pickle bed using "okara", which is secondarily produced in large quantities in the production of tofu, soybean milk, and the like. Also, a pickle bed is proposed in which lactic acid bacteria (Lactobacillus plantarum) are added to the residue of beans, grains or potatoes and fermented at a low temperature. However, the marketability of the known lactic acid bacterium maturing pickles bed is extremely limited because harmful bacteria generate CO 2 and ethylene gas due to generation of ethylene gas.

更に、公知の漬物床からは、乳酸菌が生成する乳酸と塩分が加わり、漬物床の原料の有する栄養分、蛋白質の所望の旨みが得られない。 Furthermore, the known pickle bed adds lactic acid and salt content produced by lactic acid bacteria, and the desired taste of nutrients and protein of the raw material of the pickle bed cannot be obtained.

加えて、公知の漬物床は、熱処理していないので一般細菌、特に最悪の場合はクロストリジウム属(Clostridium)、大腸菌(Escherichia Coli, colon bacillus)が発生することがあり、それらのコロニー形成が見られる。 In addition, since the known pickle bed is not heat-treated, general bacteria, especially Clostridium and Escherichia coli (Escherichia coli, colon bacillus) may occur in the worst case, and their colonization is observed. .

以上に鑑みて、特開2003-47398号公報では、従来のものの問題点を解消した漬物床が提案されている。この出願で提案された漬物床の製造法は、純粋培養でなく、“おから”に乳酸菌(ラクトバチルス ファーメンタムLactobacillus fermentum)を天然塩、昆布及び唐辛子と共に混合して培養する混合培養により乳酸醗酵して熟成させ、該熟成混合物を所要温度で所要時間煮沸して滅菌(増殖性を持つあらゆる微生物を完全に殺菌又は除去する状態を実現するための作用・操作)し、該滅菌混合物を自然冷却させ、予め粉体乳酸菌を内蔵する小袋を入れた所要外袋内に、該滅菌混合物を有する該内袋を入れてシールする乳酸菌により熟成させる漬物床の製造法である。この漬物床の製造法によれば、上記した従来の漬物床の製造法の問題点を解消することができる。 In view of the above, Japanese Unexamined Patent Application Publication No. 2003-47398 proposes a pickle bed that solves the problems of the conventional ones. The method for producing pickled vegetables proposed in this application is not a pure culture, but a mixed culture in which lactic acid bacteria (Lactobacillus fermentum) are mixed with natural salt, kelp and chili peppers and cultured. Then, the aged mixture is boiled at the required temperature for the required time to sterilize (action and operation for realizing a state in which all proliferative microorganisms are completely sterilized or removed), and the sterilized mixture is naturally cooled. Then, the inner bag containing the sterilized mixture is placed in a required outer bag containing a small bag containing powdered lactic acid bacteria in advance, and the inner bag is sealed. According to this pickle bed manufacturing method, the above-described problems of the conventional pickle bed manufacturing method can be resolved.

しかしながら、この公報で開示された漬物床の製造法においては、熟成混合物を所要温度(実施例では85℃)で、所要時間煮沸して滅菌しているので、再び乳酸菌を添加する形をとっており、作業に時間が掛かるとともに、乳酸菌再添加という追加のコストが掛かるという問題が生ずる。さらには、従来と同様に、あまり時間を経ずに乳酸菌が過多になるため、早期に酸っぱくなり、漬物床が使い物にならなくなってしまうという問題がある。 However, in the method for producing the pickle bed disclosed in this publication, the aged mixture is sterilized by boiling at the required temperature (85° C. in the example) for the required time, so lactic acid bacteria are added again. As a result, there arises a problem that the work takes time and an additional cost for re-addition of lactic acid bacteria is required. Furthermore, as in the conventional method, the lactic acid bacteria become excessive in a short period of time, so there is a problem that the pickles become sour early and the pickle bed becomes unusable.

特開2003-47398号公報 JP-A-2003-47398

そこで、本発明は、長期間酸っぱくならずに漬物を付けることができ、しかも追加の乳酸菌が必要ない新規ぬか床材料およびその製造方法ならびにぬか床を提供することを目的とする。 SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a novel rice bran bed material, a method for producing the same, and a rice bran bed which can be pickled for a long period of time without the need for additional lactic acid bacteria.

上記課題は、下記(1)~(3)の構成のぬか床材料の製造方法およびぬか床の製造方法により達成される。
(1)
米ぬかを主原料とし、2℃~15℃の温度条件下で使用されるぬか床のためのぬか床材料の製造方法であって、
米ぬかに対して、所定量のラクトバチルス ファーメンタム(Lactobacillus fermentum)である乾燥状態の乳酸菌を添加し、
これを室温下で所定時間発酵させ、
次いで、高温加熱温度68~82℃の温度下で、10~35分加熱処理し、
この加熱処理後、急速冷却し、
米ぬか1グラムについて、
生きている乳酸菌を、0.4×10~1.0×10 2 個、および
活性酵素を
含有し、
酵母および一般細菌を実質的に含有しない
ぬか床材料を製造する
ことを特徴とするぬか床材料の製造方法。
(2)
前記(1)のぬか床材料の製造方法を用いて製造されたたぬか床材料を用いてぬか床を製造するぬか床の製造方法。
(3)
塩分が無添加の、前記(2)のぬか床の製造方法。
The above objects are achieved by a method for producing bran bed material and a method for producing rice bran bed having the following configurations (1) to (3).
(1)
A method for producing a bran bed material for bran beds that uses rice bran as the main raw material and is used under a temperature condition of 2 ° C. to 15 ° C.,
A predetermined amount of dry lactic acid bacteria, which is Lactobacillus fermentum, is added to the rice bran,
This is fermented at room temperature for a predetermined time,
Then, heat treatment at a high temperature of 68 to 82 ° C. for 10 to 35 minutes,
After this heat treatment, it is rapidly cooled,
For 1 gram of rice bran,
containing 0.4 ×10 2 to 1.0× 10 2 live lactic acid bacteria and active enzymes,
A method for producing a rice bran bed material, characterized by producing a rice bran bed material substantially free of yeast and common bacteria.
(2)
A method for producing rice bran bed using the rice bran bed material produced by the method for producing rice bran bed material of ( 1) above.
(3)
The method for producing the rice bran bed according to (2) above, wherein no salt is added.

本発明のぬか床材料においては、その含有する乳酸菌の数が低く制限されており、しかも当該乳酸菌を用いたぬか床は、冷蔵庫等で実現できる低温下使用されることを前提としているので、漬物が酸っぱくならずに長期間(通常の家庭で約2ヶ月間)使用できる。一方、変性による失活が無い酵素が多量に存在しているので、これにより、風味のある漬物が可能である。また、熱処理により、有害な一般細菌(雑菌)を含んでいないので、塩分を一切使用しないでも、一般細菌(雑菌)が繁殖せず安全で風味の良い漬物ができる。なお、塩分を好む場合には、ぬか床に多少の塩を加えたり、漬け込む前に野菜等を塩揉みしてもよい。 In the rice bran bed material of the present invention, the number of lactic acid bacteria contained is limited to a low level, and the rice bran bed using the lactic acid bacteria is premised on being used at a low temperature that can be realized in a refrigerator or the like. can be used for a long time (about 2 months in a normal home) without turning sour. On the other hand, since there is a large amount of enzymes that are not deactivated by denaturation, flavorful pickles are possible. In addition, since it does not contain harmful general bacteria (abnormal bacteria) due to heat treatment, general bacteria (abnormal bacteria) do not propagate and safe and flavorful pickles can be produced without using any salt. If you prefer saltiness, you may add some salt to the nukadoko, or rub the vegetables with salt before pickling.

まず、本発明の実施の形態によるぬか床材料の製造方法について説明する。
このぬか床材料の製造にあたっては、まず、次の配合の原料を準備する。
煎り米ぬか:50~70重量%、ソヤフイット(大豆微粉末):10~30重量%、乳酸菌(乾燥状態):1~4重量%、精製食塩:4~8重量%、その他(イヌリン、トレハロース等)。
First, a method for producing bran bed material according to an embodiment of the present invention will be described.
In the production of this nukadoko material, first, raw materials having the following composition are prepared.
Roasted rice bran: 50-70% by weight, soyahuit (soybean powder): 10-30% by weight, lactic acid bacteria (dry state): 1-4% by weight, refined salt: 4-8% by weight, others (inulin, trehalose, etc.) .

前記乳酸菌としては、従来から醗酵乳或いは漬物等に使用されているラクトバチルス(Lactobacillus)属、ペジオコッカス(Pediococcus)属、ロイコノストック(Leuconostoc)属、ビフィドバクテリウム(Bifidobacterium)属等の微生物を用いることができ、ラクトバチルス属としては、ラクトバチルスカゼイ(Lactobacillus casei)、ラクトバチルス ファーメンタム(Lactobacillus fermentum)、ラクトバチルス ブレビス(Lactobacillus brevis)、ラクトバチルス アラビノサス(Lactobacillus arabinosus)、ラクトバチルス ラ・フランス 等があげられるが、本発明で使用される乳酸菌としては、ヘテロ型乳酸菌であるラクトバチルス ファーメンタムが特に好ましい。 As the lactic acid bacteria, microorganisms such as Lactobacillus genus, Pediococcus genus, Leuconostoc genus, Bifidobacterium genus, which are conventionally used for fermented milk or pickles, etc. Examples of the genus Lactobacillus include Lactobacillus casei, Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus arabinosus, Lactobacillus arabinosus, etc. Lactobacillus fermentum, which is a heterozygous lactic acid bacterium, is particularly preferred as the lactic acid bacterium used in the present invention.

上記のように配合した原料100重量部に対して、水を600~700重量部加え、室温下で約5~10時間程度熟成させる。このとき、乳酸菌の数は2.0×10個~1.2×1010個程度に増殖し、これに伴って、乳酸菌が酵素を産出する。 600 to 700 parts by weight of water is added to 100 parts by weight of the raw material blended as described above, and the mixture is aged at room temperature for about 5 to 10 hours. At this time, the number of lactic acid bacteria grows to about 2.0×10 9 to 1.2×10 10 , and accordingly the lactic acid bacteria produce enzymes.

この後、上記熟成したぬか床材料を下記条件で加熱処理して、減菌する。
温度:68~82℃
時間:10~35分
上記熱処理後、急速冷却することにより、ぬか床材料を完成させる。この時袋に入って製造されていることから、梱包・ラベル貼りして製品となる。
Thereafter, the aged rice bran bed material is heat-treated under the following conditions to sterilize it.
Temperature: 68-82°C
Time: 10 to 35 minutes After the heat treatment, the rice bran bed material is completed by rapid cooling. At this time, since it is manufactured in a bag, it is packaged and labeled to become a product.

以上により、
米ぬか1グラムについて、
生きている乳酸菌を、好ましくは0.2×10~1×10個、特に好ましくは、1×10~1×10個および活性酵素を、4000力価係数~5000力価係数を含有するぬか床材料が製造される。
このぬか床材料は、前記熱処理により、酵母および一般細菌(有害な雑菌)が減菌され、それらを実質的に含有しない。
From the above,
For 1 gram of rice bran,
Live lactic acid bacteria, preferably 0.2×10 2 to 1×10 5 , particularly preferably 1×10 2 to 1×10 3 , and active enzymes with a titer factor of 4000 to 5000. A bran bed material containing is produced.
This bran bed material is sterilized by the heat treatment, and contains substantially no yeast and general bacteria (harmful germs).

まず、米ぬか1kgに乳酸菌(乾燥状態)を6g添加し、これに水691kgを添加して、8時間熟成させ、原料を準備した。このとき、原料1gにつき、乳酸菌が5.8×10個、酵母菌が500個以下、一般細菌が5.4×10個であった。 First, 6 g of lactic acid bacteria (dry state) was added to 1 kg of rice bran, 691 kg of water was added, and the mixture was aged for 8 hours to prepare a raw material. At this time, 5.8×10 6 lactic acid bacteria, 500 or less yeast, and 5.4×10 6 general bacteria were found per 1 g of raw material.

上記原料を、下記のように温度と、処理時間を変えて熱処理をして、実施例と比較例のぬか床材料サンプルを作成した。熱処理後、乳酸菌数、酵母数、一般細菌数を測定した。結果を下記の表に示した。

Figure 0007193075000001
The above raw materials were heat-treated at different temperatures and treatment times as described below to prepare rice bran bed material samples of Examples and Comparative Examples. After heat treatment, the number of lactic acid bacteria, the number of yeasts, and the number of general bacteria were measured. The results are shown in the table below.
Figure 0007193075000001

上記実施例と比較例のぬか床材料へ、下記の種類の添加物を、下記の配合割合で添加してぬか床サンプルを作成した。
米ぬか 1kgに対して
(1)イヌリン(菊芋微粉末) 7%
(2)トレハロース(発酵資材) 3%

To the bran bed materials of the above Examples and Comparative Examples, the following types of additives were added in the following mixing ratios to prepare bran bed samples.
per 1 kg of rice bran
(1) Inulin (Jerusalem artichoke fine powder) 7%
(2) Trehalose (fermented material) 3%

上記ぬか床サンプルとしては、「できたて(1日)」、および「できてから30日」のものに、実際キュウリを塩揉みせずに100本ずつそれぞれ10時間漬け込み、100名の男女にモニターとなって貰い味見テスト(官能テスト)を行った。
その評価結果を前記表に、◎、○、△、×ので示した。「できたて(1日)」のものを評価1に、および「できてから30日」のものを評価2に示した。
◎、○、△、×の意味は、それぞれ「大変に美味しい」、「美味しい」、「変化なし」、「酸ぱい」とした。その表示は、100名のモニターの中で、評価する人が一番多いものをその評価とした。
例えば、◎が40名、○が30名、△が20名、×が10名の場合には、一番人数の多い◎とした。
As the above-mentioned nukadoko samples, 100 cucumbers were soaked for 10 hours each in "freshly made (1 day)" and "30 days after being made" without actually rubbing salt, and 100 men and women were tested. A tasting test (sensory test) was performed by having them serve as a monitor.
The evaluation results are indicated by ⊚, ◯, Δ, and × in the above table. A rating of 1 was given for "freshly made (1 day)" and a rating of 2 was given for "30 days after making".
The meanings of ⊚, ∘, Δ, and × are “very delicious,” “tasty,” “no change,” and “sour,” respectively. As for the display, the one evaluated by the largest number of people among 100 monitors was used as the evaluation.
For example, if ⊙ is 40, O is 30, Δ is 20, and × is 10, then ⊚ is the largest number.

なお、モニターの人数構成は下記の通りとした。
小学生:男女10名ずつ
中学生:男女10名ずつ
高校生:男女10名ずつ
30代:男女10名ずつ
60代::男女10名ずつ
The composition of the number of monitors was as follows.
Elementary school students: 10 men and women Junior high school students: 10 men and women High school students: 10 men and women 30s: 10 men and women 60s: 10 men and women

比較例4のできて直ぐのぬか床サンプルで浅漬けたものは、ぬか臭いというモニターが多数いた。一方、比較例1のできてから30日のものは、酸っぱすぎるというモニターが多数いた。
以上から、本発明の効果が明らかである。
なお、前記熱処理の条件を、70℃で35分とした場合は、実施例3とほぼ同様の結果が得られ、一方、70℃で5分とした場合は、乳酸菌の数が多くなりすぎあまり良い結果が得られなかった。
Many monitors said that the lightly pickled rice bran bed sample of Comparative Example 4 had a rice bran smell. On the other hand, there were many monitors who said that the product of Comparative Example 1 after 30 days was too sour.
From the above, the effect of the present invention is clear.
When the heat treatment was performed at 70°C for 35 minutes, almost the same results as in Example 3 were obtained. I didn't get good results.

Claims (3)

米ぬかを主原料とし、2℃~15℃の温度条件下で使用されるぬか床のためのぬか床材料の製造方法であって、
米ぬかに対して、所定量のラクトバチルス ファーメンタム(Lactobacillus fermentum)である乾燥状態の乳酸菌を添加し、
これを室温下で所定時間発酵させ、
次いで、高温加熱温度68~82℃の温度下で、10~35分加熱処理し、
この加熱処理後、急速冷却し、
米ぬか1グラムについて、
生きている乳酸菌を、0.4×10~1.0×10 2 個、および
活性酵素を
含有し、
酵母および一般細菌を実質的に含有しない
ぬか床材料を製造する
ことを特徴とするぬか床材料の製造方法。
A method for producing a bran bed material for bran beds that uses rice bran as the main raw material and is used under a temperature condition of 2 ° C. to 15 ° C.,
A predetermined amount of dry lactic acid bacteria, which is Lactobacillus fermentum, is added to the rice bran,
This is fermented at room temperature for a predetermined time,
Then, heat treatment at a high temperature of 68 to 82 ° C. for 10 to 35 minutes,
After this heat treatment, it is rapidly cooled,
For 1 gram of rice bran,
containing 0.4 ×10 2 to 1.0× 10 2 live lactic acid bacteria and active enzymes,
A method for producing a rice bran bed material, characterized by producing a rice bran bed material substantially free of yeast and common bacteria.
請求項1のぬか床材料の製造方法を用いて製造されたたぬか床材料を用いてぬか床を製造するぬか床の製造方法。 A method for producing rice bran bed using the rice bran bed material produced by the method for producing rice bran bed material according to claim 1 . 塩分が無添加の、請求項のぬか床の製造方法。 The method for producing bran bed according to claim 2 , wherein no salt is added.
JP2018112473A 2018-06-13 2018-06-13 Nukadoko material, its manufacturing method, and nukadoko Active JP7193075B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2018112473A JP7193075B2 (en) 2018-06-13 2018-06-13 Nukadoko material, its manufacturing method, and nukadoko

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2018112473A JP7193075B2 (en) 2018-06-13 2018-06-13 Nukadoko material, its manufacturing method, and nukadoko

Publications (2)

Publication Number Publication Date
JP2019213487A JP2019213487A (en) 2019-12-19
JP7193075B2 true JP7193075B2 (en) 2022-12-20

Family

ID=68919090

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018112473A Active JP7193075B2 (en) 2018-06-13 2018-06-13 Nukadoko material, its manufacturing method, and nukadoko

Country Status (1)

Country Link
JP (1) JP7193075B2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001095477A (en) 1999-10-04 2001-04-10 Okabeya:Kk Production of pickle bed
JP2005080556A (en) 2003-09-08 2005-03-31 Mc Foodtech Kk Rapidly fermented salted rice-bran paste composition
JP3117167U (en) 2005-09-29 2006-01-05 株式会社丸和製作所 Bran pickle container

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001095477A (en) 1999-10-04 2001-04-10 Okabeya:Kk Production of pickle bed
JP2005080556A (en) 2003-09-08 2005-03-31 Mc Foodtech Kk Rapidly fermented salted rice-bran paste composition
JP3117167U (en) 2005-09-29 2006-01-05 株式会社丸和製作所 Bran pickle container

Also Published As

Publication number Publication date
JP2019213487A (en) 2019-12-19

Similar Documents

Publication Publication Date Title
JP2022027691A (en) cream
JP5044769B2 (en) Lactic acid bacteria Lactobacillus sakei strain, beverage manufacturing method, food manufacturing method, pickled bed manufacturing method, bread making raw material manufacturing method
JP6858406B2 (en) Lactic acid bacteria composition, fermented milk and its production method, and fermented milk production kit
JP2007175024A (en) Method for producing tempeh-like fermented food
CN108118011A (en) A kind of instant pickle preparation method of less salt
JP4004531B1 (en) Manufacturing method of fermented seasoning
CN105580893A (en) Stirring-type highland barley yoghourt and preparation method thereof
JP2005304493A (en) Method for production of raw material for food
JP4673786B2 (en) Method for producing yogurt with fruits and vegetables
JP7193075B2 (en) Nukadoko material, its manufacturing method, and nukadoko
Swinehart et al. Food Safety Implications of Online Recipes for Preparing Soaked Nuts and Nut-Based Dairy Analogs.
JP5616655B2 (en) Grain flour processed product and method for producing grain flour processed product
JP3913200B2 (en) Removal method of unpleasant taste and unpleasant odor of vegetables and / or beans and processed food obtained using the same
KR101022797B1 (en) Manufacturing method of soybean yogurt by using sotbean milk and the mixture of lactic acid bacteria, and soybean yogurt made by the same
JP2018082695A (en) Antibacterial lactic-acid-bacterium strain suppressing bacillus bacterial proliferation, and food production method sing the same
JP6360346B2 (en) Method for producing fermented product using natural fermentation system and method for expanding and using the fermented product and bacterial flora
JPH10313814A (en) Production of miso
JPH1175754A (en) Production of fermented seasoning
KR101334803B1 (en) Preparation method for easy-to-use natural watery kimchi seasoning powder and formulation of the same
JP2018164455A (en) Application product formed by using fermentation product using natural fermentation system
JP4885802B2 (en) Method for producing salt-free or low-salt miso seasoning and its product
JP2008154534A (en) Method for producing lactobacillus fermented rice bran
JP4205006B2 (en) Pickled floor and method of manufacturing pickles using the same
US1583294A (en) Manufacture of butter substitutes
JP6818276B1 (en) Liquid bran floor forming agent

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180613

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210611

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210624

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20220419

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220510

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220707

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221025

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221026

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20221115

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20221130

R150 Certificate of patent or registration of utility model

Ref document number: 7193075

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150