JP2008154534A - Method for producing lactobacillus fermented rice bran - Google Patents
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本発明は、米ぬかを原料とし、乳酸菌により発酵させて乳酸菌発酵米ぬかを得る乳酸菌発酵米ぬかの製造方法に関するものである。 The present invention relates to a method for producing lactic acid bacteria-fermented rice bran, which uses rice bran as a raw material and is fermented with lactic acid bacteria to obtain lactic acid bacteria-fermented rice bran.
米ぬかは、玄米を精白する際に生成する副産物で、ビタミン・ミネラル・食物繊維を多く含み栄養的にも豊富な食品素材であり、更年期障害を抑制する作用のあるγ−オリザノール、抗酸化剤であるフェルラ酸など様々な機能性物質を含んでいる。 Rice bran is a by-product that is produced when white rice is refined, and is a food material that is rich in vitamins, minerals and dietary fiber and is rich in nutrition, and is a γ-oryzanol and antioxidant that has the effect of inhibiting menopause. It contains various functional substances such as certain ferulic acid.
また、この米ぬかにはアラビノキシランという多糖類が多く含まれていることが知られている。アラビノキシランはアラビノースとキシロースを構成糖とする多糖類であり、この多糖類には抗癌作用、免疫賦活作用があると言われている。この多糖類のうち分子量の大きいものに抗癌作用、免疫賦活作用があるという説と、分解され分子量が小さくなったものに抗癌作用、免疫賦活作用があるという説とがあり意見が分かれている。 Moreover, it is known that this rice bran contains a large amount of a polysaccharide called arabinoxylan. Arabinoxylan is a polysaccharide comprising arabinose and xylose as constituent sugars, and it is said that this polysaccharide has an anticancer action and an immunostimulatory action. There is an opinion that there are theories that among these polysaccharides, those with a high molecular weight have anticancer activity and immunostimulatory activity, and those that have been decomposed and reduced in molecular weight have anticancer activity and immunostimulatory activity. Yes.
このように米ぬかは、非常に有用な食品素材であり、その活用が望まれている。 Thus, rice bran is a very useful food material, and its utilization is desired.
しかしながら米ぬかは、(1)製造後の安定性・保存性が悪い、(2)食感や臭いが悪い、(3)消化・吸収が悪い、などの欠点がある。 However, rice bran has drawbacks such as (1) poor stability and storage after production, (2) poor texture and smell, and (3) poor digestion and absorption.
特に「安定性・保存性が悪い」「食感や臭いが悪い」という欠点は、生の米ぬかには微生物が多く含まれていることと米ぬかには脂質が多いため内因性のリパーゼで油脂が加水分解され酸敗し「ぬか臭」が発生することに起因する。 In particular, the disadvantages of "poor stability and storage" and "bad texture and smell" are that raw rice bran contains a lot of microorganisms and rice bran contains a lot of lipids, so fats and oils are endogenous lipase. It is caused by hydrolysis, acidification, and a “branch odor”.
そこで、米ぬかを原料として乳酸菌により発酵させた乳酸菌発酵米ぬかの製造が試みられてきた。 Therefore, production of lactic acid bacteria fermented rice bran fermented with lactic acid bacteria using rice bran as a raw material has been attempted.
特に米ぬかを乳酸菌で発酵させることにより、このアラビノキシランが酸分解したものか、あるいは微弱なヘミセルラーゼ活性により若干分解したものが生じると考えられる。また乳酸発酵により米ぬかからアラビノキシランの溶出が容易になると考えられ、乳酸発酵させることにより未発酵の米ぬかより吸収されやすいアラビノキシランを生成させることが可能となる。 In particular, fermenting rice bran with lactic acid bacteria may result in acid degradation of this arabinoxylan or a slight degradation due to weak hemicellulase activity. In addition, it is considered that arabinoxylan is easily eluted from rice bran by lactic acid fermentation, and arabinoxylan that is more easily absorbed than unfermented rice bran can be produced by lactic acid fermentation.
しかしながら、従来このような効能を目的として乳酸発酵における様々な手法も提案されてきてはいるが、いずれも米ぬかの乳酸発酵が良好に行われなかったり、前記アラビノキシランが十分に生成されなかったりして、前記(1)〜(3)の米ぬかの欠点が十分に解決されず十分な効果が得られなかったり、また製造が厄介となったり、コスト高となるなど夫々様々な問題があった(特開2001−238664公報など)。 However, various methods in lactic acid fermentation have been proposed for the purpose of such effects, but none of them has been successfully performed in lactic fermentation of rice bran or the arabinoxylan is not sufficiently produced. However, the problems of (1) to (3) were not solved sufficiently, so that a sufficient effect was not obtained, the production was troublesome, and the cost was high. No. 2001-238664).
本発明は、米ぬかの健康有効成分を食品として有効に活用でき、且つ乳酸菌による乳酸発酵が良好に行われることで米ぬかの欠点であった、前記(1),(2),(3)などの欠点が解決され、安定性・保存性に秀れ、臭みもなく、消化吸収も良く、しかも製造も簡易に行え、純植物性の乳酸菌発酵米ぬかを容易に実現できる画期的な乳酸菌発酵米ぬかの製造方法を提供することを目的としている。 The present invention is a rice bran deficiency that can effectively use the health ingredients of rice bran as a food and that lactic acid fermentation by lactic acid bacteria is performed well, such as the above (1), (2), (3), etc. Innovative lactic acid bacteria fermented rice bran that solves the shortcomings, is excellent in stability and storage, has no odor, is easy to digest and absorb, can be easily manufactured, and can easily realize pure plant lactic acid bacteria fermented rice bran The object is to provide a manufacturing method.
添付図面を参照して本発明の要旨を説明する。 The gist of the present invention will be described with reference to the accompanying drawings.
米ぬかに、植物質由来の植物性乳酸菌を接種して乳酸発酵させ乳酸菌発酵米ぬかを製造することを特徴とする乳酸菌発酵米ぬかの製造方法に係るものである。 The present invention relates to a method for producing lactic acid bacteria fermented rice bran, which comprises inoculating plant lactic acid bacteria derived from plant matter and fermenting them with lactic acid to produce lactic acid bacteria fermented rice bran.
また、前記植物性乳酸菌として、少なくとも米・麦などの穀物由来の乳酸菌と、りんご・みかんなどの果物由来の乳酸菌とを用いて複合発酵させ、前記穀物由来の乳酸菌と前記果物由来の乳酸菌との量の設定により風味を設定したことを特徴とする請求項1記載の乳酸菌米ぬかの製造方法に係るものである。 Further, as the plant lactic acid bacteria, combined fermentation is performed using at least lactic acid bacteria derived from grains such as rice and wheat and lactic acid bacteria derived from fruits such as apples and tangerines, and the lactic acid bacteria derived from the grains and the lactic acid bacteria derived from the fruits 2. The lactic acid bacteria rice bran manufacturing method according to claim 1, wherein the flavor is set by setting the amount.
本発明は上述のように構成したから、米ぬかの健康有効成分を食品として有効に活用でき、且つ乳酸菌による乳酸発酵が良好に行われることで米ぬかの欠点であった、前記(1),(2),(3)などの欠点が解決され、安定性・保存性に秀れ、臭みもなく、消化吸収も良く、しかも製造も簡易に行え、純植物性の乳酸菌発酵米ぬかを容易に実現できる画期的な乳酸菌発酵米ぬかの製造方法となる。 Since the present invention is configured as described above, the health ingredients of rice bran can be effectively used as food, and the above-mentioned (1), (2) has been a disadvantage of rice bran due to good lactic acid fermentation by lactic acid bacteria. ), (3), etc. are solved, excellent in stability and storage, no odor, good digestion and absorption, easy to manufacture, and easy to produce pure vegetable lactic acid bacteria fermented rice bran It becomes the production method of seasonal lactic acid bacteria fermentation rice bran.
また、請求項2記載の発明においては、例えば穀物由来の乳酸菌で発酵を行うと、濃厚な味わいとなり、また果物由来の乳酸菌で発酵を行うと、エグミが少なく爽やかで酸味が少なくさっぱりとした味となり、野菜由来の乳酸菌ではこの中間の風味となることから、少なくとも穀物由来の乳酸菌と果物由来の乳酸菌とを混ぜ複合発酵させ、この分量を設定することで味の調整を行うことが可能で、他の諸条件や本製品の使用用途や消費者のニーズに応じて味の設定も可能となる一層秀れた乳酸菌発酵米ぬかの製造方法となる。 In the invention of claim 2, for example, when fermented with lactic acid bacteria derived from grains, a rich taste is obtained, and when fermented with lactic acid bacteria derived from fruits, the taste is less refreshing, less sour and less refreshed. It is possible to adjust the taste by mixing and fermenting at least lactic acid bacteria derived from fruits and lactic acid bacteria derived from fruits, and setting this amount. This is a more excellent lactic acid bacteria fermented rice bran manufacturing method that allows the taste to be set according to other conditions, the intended use of the product and the needs of consumers.
好適と考える本発明の実施形態(発明をどのように実施するか)を、図面に基づいて本発明の作用を示して簡単に説明する。 DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention that are considered suitable (how to carry out the invention) will be briefly described with reference to the drawings, illustrating the operation of the present invention.
本発明は、牛乳をチーズとするときに用いる動物性乳酸菌を用いず、植物性乳酸菌を用いて米ぬかを乳酸発酵する。 The present invention does not use animal lactic acid bacteria used when milk is used as cheese, but lactic acid ferments rice bran using plant lactic acid bacteria.
植物性乳酸菌は、植物質由来の乳酸菌であり、例えば穀物や果物や野菜やこれらから得られるものなどの植物質由来の乳酸菌であり、動物性乳酸菌を選定せずこの植物性乳酸菌を選定してこれを米ぬかに接種して発酵させることで、米ぬかの乳酸発酵が極めて良好に行われ、米ぬかの欠点であった前述の(1),(2),(3)の欠点などが解決され、安定性・保存性に秀れ、臭みもなく、消化吸収も良く、しかも製造も簡易に行え、純植物性の乳酸菌発酵米ぬかの製造を容易に実現できることとなる。 Plant lactic acid bacteria are plant-derived lactic acid bacteria, for example, lactic acid bacteria derived from plants such as cereals, fruits and vegetables, and those obtained from these, and this plant lactic acid bacterium is selected without selecting animal lactic acid bacteria. By inoculating this with rice bran and fermenting it, lactic acid fermentation of rice bran was performed very well, and the above-mentioned drawbacks of (1), (2) and (3), which were the disadvantages of rice bran, were solved and stable. It has excellent properties and preservability, has no odor, good digestion and absorption, can be easily manufactured, and can easily produce pure plant lactic acid bacteria fermented rice bran.
即ち、乳酸菌による発酵でありながらも植物質由来の乳酸菌を選定することにより、米ぬかの乳酸菌による乳酸発酵が良好に進み、米ぬかより吸収され易いアラビノキシランが生成あるいはその溶出が容易となり、安定性・保存性に秀れ、臭みがないだけでなく、消化・吸収にも秀れ、米ぬかの健康有効成分をいかしつつ米ぬかの独特な風味もあり、乳酸発酵により美味さも十分に引き出され、健康イメージの強い純植物性の乳酸菌発酵米ぬかを簡易な手法によって大幅なコスト高となることなく実現できる画期的な乳酸菌発酵米ぬかの製造方法となる。 In other words, by selecting plant-derived lactic acid bacteria while being fermented by lactic acid bacteria, lactic acid fermentation by rice bran lactic acid bacteria proceeds well, and arabinoxylan that is easily absorbed by rice bran is easily generated or eluted, which is stable and preserved. Not only does it excel in nature, it does not smell, it also excels in digestion and absorption, has a unique flavor of rice bran while utilizing the active ingredients of rice bran, and has a strong health image that is fully extracted by lactic acid fermentation. This is a revolutionary lactic acid bacteria fermented rice bran production method that can realize pure plant lactic acid bacteria fermented rice bran by a simple method without significant cost increase.
また、例えば穀物由来の乳酸菌で発酵を行うと、乳酸菌発酵米ぬかは濃厚な味わいとなり、また果物由来の乳酸菌で発酵を行うと、エグミが少なく爽やかで酸味が少なくさっぱりとした味となり、野菜由来の乳酸菌ではこの中間の風味となる。 For example, when fermented with lactic acid bacteria derived from cereals, lactic acid bacteria fermented rice bran has a rich taste, and when fermented with lactic acid bacteria derived from fruits, it has a fresh taste with a little freshness and less sourness. Lactic acid bacteria have this intermediate flavor.
このように植物性乳酸菌は、米ぬかの乳酸発酵に秀れると共に、菌種の種類がバラエティーであり、味を様々に変えやすいという特性を持つ。 As described above, the plant lactic acid bacteria are excellent in lactic acid fermentation of rice bran, have a variety of bacterial species, and have a characteristic of easily changing the taste.
また、例えば穀物由来の乳酸菌と果物由来の乳酸菌とを混ぜ複合発酵させれば、味の調整を行うことが可能で、他の諸条件や本製品の使用用途や消費者のニーズに応じてこの分量を調整設定して味の設定も可能となる。 In addition, for example, it is possible to adjust the taste by mixing lactic acid bacteria derived from cereals and lactic acid bacteria derived from fruits and fermenting them together, and this can be adjusted according to other conditions, the intended use of this product, and consumer needs. The taste can be set by adjusting the amount.
本発明の具体的な本実施例について図面に基づいて説明する。 Specific examples of the present invention will be described with reference to the drawings.
本実施例では、米ぬかを殺菌処理し、これに植物性乳酸菌を加えて(接種して)乳酸発酵させて乳酸菌発酵米ぬかを得て、この乳酸菌発酵米ぬかを自動酸化防止のために、アルミパウチ袋に脱酸素剤を入れて充填封入し、冷蔵保存する。 In this embodiment, the rice bran is sterilized, and plant lactic acid bacteria are added (inoculated) to the lactic acid fermentation to obtain lactic acid bacteria fermented rice bran, and this lactic acid bacteria fermented rice bran is used in an aluminum pouch bag for automatic oxidation prevention. Fill with oxygen scavenger, and store in a refrigerator.
以下具体的に説明する。 This will be specifically described below.
1 原料米ぬかの殺菌
原料となる米ぬかは高温短時間殺菌済み米ぬかを使用する。米ぬかに高温短時間殺菌処理を施すことにより、米ぬか中の初発菌数を少なくし、且つ内因性のリパーゼを失活させる。
1 Sterilization of raw rice bran Use raw rice bran that has been sterilized at high temperature and short time. By subjecting rice bran to high-temperature and short-term sterilization, the number of initial bacteria in rice bran is reduced and endogenous lipase is inactivated.
例えば、この高温短時間の殺菌処理条件は、蒸気殺菌で100℃、20分間行う。 For example, the high temperature and short time sterilization conditions are steam sterilization at 100 ° C. for 20 minutes.
以上の前処理を行うことにより安定的に乳酸菌発酵米ぬか(HOT-Lab Bran)の製造が可能となる。 By performing the above pretreatment, it is possible to stably produce lactic acid bacteria fermented rice bran (HOT-Lab Bran).
2 乳酸発酵
米ぬかの乳酸発酵のために純粋培養の乳酸菌を一株或いは数株接種する。乳酸菌は米ぬかの発酵に適した植物性乳酸菌を用い、米ぬか重量に対し、10%程度添加して発酵させる。米ぬかを乳酸発酵させることにより吸収性の良いアラビノキシランの生成が可能となる。
2. Lactic acid fermentation One or several strains of purely cultured lactic acid bacteria are inoculated for lactic acid fermentation of rice bran. Lactic acid bacteria are plant lactic acid bacteria suitable for rice bran fermentation and are fermented by adding about 10% of the rice bran weight. By lactic acid fermentation of rice bran, it is possible to produce arabinoxylan with good absorbability.
例えば、具体的には、30℃で3日から7日間発酵させる。 For example, specifically, it is fermented at 30 ° C. for 3 to 7 days.
また、前述のように殺菌処理した殺菌米ぬかを乳酸発酵させることにより、発酵が正常に進行し、乳酸発酵特有の芳香のある乳酸菌発酵米ぬかが出来る。 Further, by lactic acid fermentation of the sterilized rice bran that has been sterilized as described above, the fermentation proceeds normally, and lactic acid bacteria fermented rice bran having an aroma unique to lactic acid fermentation can be obtained.
尚、本実施例での発酵終了後の乳酸菌数は1.6×108個/g、発酵終了後のpHは、4.65であった。 In this example, the number of lactic acid bacteria after the fermentation was 1.6 × 10 8 cells / g, and the pH after the fermentation was 4.65.
3 充填・冷蔵保存
米ぬかの自動酸化を防止するため、前記発酵後、アルミパウチ袋に脱酸素剤と共に封入し、冷蔵庫にて保存する。
3 Filling and refrigerated storage In order to prevent the automatic oxidation of rice bran, after the fermentation, it is sealed with an oxygen scavenger in an aluminum pouch bag and stored in a refrigerator.
4 製パンに用いた実施例
前記本実施例により製造した乳酸菌発酵米ぬかをパン生地に混合し、乳酸菌発酵米ぬか入りパンを製造した実施例について更に説明する。
4. Example used for bread-making An example in which lactic acid bacteria-fermented rice bran produced according to the present example was mixed with bread dough to produce bread containing lactic acid bacteria-fermented rice bran will be further described.
前記実施例による乳酸菌発酵米ぬかをパン生地に混合し、乳酸菌発酵米ぬか入りパンを製造した実施例において、以下の三種類(三つの試験区)について比較した。 In the examples in which the lactic acid bacteria fermented rice bran according to the above example was mixed with bread dough to produce bread containing lactic acid bacteria fermented rice bran, the following three types (three test sections) were compared.
・未発酵米ぬか20%添加
・乳酸菌発酵米ぬか(HOT-Lab Bran)発酵一週間 10%添加
・乳酸菌発酵米ぬか(HOT-Lab Bran)発酵一週間 20%添加
また、パンの製造・成分は以下の表1に示すとおりである。
・ Add 20% unfermented rice bran ・ Add 10% lactic acid bacteria fermented rice bran (HOT-Lab Bran) for one week ・ Add 20% lactic acid bacteria fermented rice bran (HOT-Lab Bran) for one week As shown in FIG.
その結果、乳酸菌発酵米ぬかを10%添加したものが最もパンの伸びが良く、20%添加のものは伸びが控えめであった。いずれにしても未発酵米ぬかを添加したものに比べて乳酸菌発酵米ぬか添加の方がやや伸びは良かった。香りについては、未発酵米ぬか添加のパンはぬかの臭いが強く乳酸菌発酵米ぬかの方がパンの香りがあって評価が良かった。味についても未発酵米ぬか添加のパンはぬかの味があり、乳酸菌発酵米ぬか添加の方が適度な酸味があって食味評価が良かった。
5 生食実施例
本実施例の乳酸菌発酵米ぬかを加熱することなく生のまま食する手段としてこの発酵させた乳酸菌発酵米ぬかに砂糖を混合した後、丸めて団子状にしたものを試作し試食した。その結果、おからのような食感の新規な食品が出来た。
As a result, the addition of 10% lactic acid bacteria fermented rice bran had the best bread growth, and the addition of 20% had a modest growth. In any case, the addition of lactic acid bacteria fermented rice bran was slightly better than that added with unfermented rice bran. As for the fragrance, bread with unfermented rice bran added had a strong smell of bran, and the lactic acid bacteria fermented rice bran had a better bread scent and was highly evaluated. As for the taste, the bread with unfermented rice bran had a taste of bran, and the addition of lactic acid bacteria fermented rice bran had a more appropriate acidity and the taste evaluation was good.
5 Raw Food Example As a means of eating raw lactic acid bacteria fermented rice bran of this example without heating, sugar was mixed with this fermented lactic acid bacteria fermented rice bran, and then a rounded dumpling was made as a trial and sampled. As a result, a new food with a texture like okara was made.
尚、本発明は、本実施例に限られるものではなく、各構成要件の具体的構成は適宜設計し得るものである。 Note that the present invention is not limited to this embodiment, and the specific configuration of each component can be designed as appropriate.
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JP2016054649A (en) * | 2014-09-05 | 2016-04-21 | 日東富士製粉株式会社 | Bakery food raw material, method for producing bakery food, and bakery food |
KR101837163B1 (en) | 2015-11-23 | 2018-03-13 | 주식회사 지리산쌀 | Sunsik product using fermented rice bran products and manufacturing method thereby |
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JPWO2015056770A1 (en) * | 2013-10-17 | 2017-03-09 | 株式会社ゲノム創薬研究所 | New lactic acid bacteria, innate immunity activator containing new lactic acid bacteria as active ingredients, and food and drink containing new lactic acid bacteria |
JP2016054649A (en) * | 2014-09-05 | 2016-04-21 | 日東富士製粉株式会社 | Bakery food raw material, method for producing bakery food, and bakery food |
KR101837163B1 (en) | 2015-11-23 | 2018-03-13 | 주식회사 지리산쌀 | Sunsik product using fermented rice bran products and manufacturing method thereby |
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