JP7175881B2 - ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ - Google Patents

ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ Download PDF

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JP7175881B2
JP7175881B2 JP2019519212A JP2019519212A JP7175881B2 JP 7175881 B2 JP7175881 B2 JP 7175881B2 JP 2019519212 A JP2019519212 A JP 2019519212A JP 2019519212 A JP2019519212 A JP 2019519212A JP 7175881 B2 JP7175881 B2 JP 7175881B2
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luciferase
days
luciferin
seq
atp
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JP2019537434A5 (enExample
JP2019537434A (ja
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ツェー シー
トーマス カークランド
ポンチョ マイセンハイマー
ランス ピー エンセル
マリー ホール
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2019519212A 2016-10-13 2017-10-13 ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ Active JP7175881B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022162232A JP7615100B2 (ja) 2016-10-13 2022-10-07 ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ

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Application Number Priority Date Filing Date Title
US201662407815P 2016-10-13 2016-10-13
US62/407,815 2016-10-13
PCT/US2017/056571 WO2018071807A2 (en) 2016-10-13 2017-10-13 Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products

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JP2022162232A Division JP7615100B2 (ja) 2016-10-13 2022-10-07 ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ

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JP2019537434A JP2019537434A (ja) 2019-12-26
JP2019537434A5 JP2019537434A5 (enExample) 2020-11-26
JP7175881B2 true JP7175881B2 (ja) 2022-11-21

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JP2019519212A Active JP7175881B2 (ja) 2016-10-13 2017-10-13 ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ
JP2022162232A Active JP7615100B2 (ja) 2016-10-13 2022-10-07 ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ

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US (1) US11261478B2 (enExample)
EP (1) EP3526343B1 (enExample)
JP (2) JP7175881B2 (enExample)
WO (1) WO2018071807A2 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7563668B2 (ja) * 2018-06-05 2024-10-08 プロメガ コーポレイション 熱安定性ルシフェリンと共に生物発光アッセイに使用するためのルシフェラーゼ酵素
US11596895B2 (en) 2020-07-17 2023-03-07 Air Products And Chemicals, Inc. Radial adsorber, adsorption system, and adsorption methods
CN120457210A (zh) 2023-01-27 2025-08-08 龟甲万株式会社 萤光素酶突变体
CN117925547B (zh) * 2023-08-01 2024-10-11 上海碧云天生物技术股份有限公司 经改造的萤火虫萤光素酶、其制备方法及应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509051A (ja) 1999-09-15 2003-03-11 プロメガ コーポレイション フォツリス・ペンシルバニカ及びピロフィルス・プラギオフタラムス由来の熱安定性ルシフェラーゼ及び製造方法
JP2004528024A (ja) 2001-02-16 2004-09-16 プロメガ　コーポレイション 改良されたａｔｐ検出法
JP2010509924A (ja) 2006-11-20 2010-04-02 バイエル・シェーリング・ファルマ・アクチェンゲゼルシャフト 生物発光検出によるピロリン酸塩の検出方法
JP2013532958A (ja) 2010-05-11 2013-08-22 プロメガ コーポレイション 増強された検出特性を有する変異プロテアーゼバイオセンサー
US20160291000A1 (en) 2013-11-08 2016-10-06 Mcmaster University Methods of stabilizing molecules without refrigeration using water soluble polymers and applications thereof in performing chemical reactions

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535744T2 (de) 1994-01-03 2009-07-02 Promega Corp., Madison Mutierte luziferasen
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
JP3749628B2 (ja) * 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme
US7192729B2 (en) * 1999-07-06 2007-03-20 General Atomics Methods for assaying homocysteine
US7183092B2 (en) * 2003-10-03 2007-02-27 Board Of Control Of Michigan Technological University Modified luciferase
JP5188771B2 (ja) * 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
CN101861397B (zh) * 2007-11-20 2014-04-16 龟甲万株式会社 核酸分析用组合物
WO2009140662A1 (en) * 2008-05-15 2009-11-19 Illumina, Inc. Engineered luciferases
WO2012109470A2 (en) * 2011-02-09 2012-08-16 University Of Massachusetts Mutant luciferases

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509051A (ja) 1999-09-15 2003-03-11 プロメガ コーポレイション フォツリス・ペンシルバニカ及びピロフィルス・プラギオフタラムス由来の熱安定性ルシフェラーゼ及び製造方法
JP2004528024A (ja) 2001-02-16 2004-09-16 プロメガ　コーポレイション 改良されたａｔｐ検出法
JP2010509924A (ja) 2006-11-20 2010-04-02 バイエル・シェーリング・ファルマ・アクチェンゲゼルシャフト 生物発光検出によるピロリン酸塩の検出方法
JP2013532958A (ja) 2010-05-11 2013-08-22 プロメガ コーポレイション 増強された検出特性を有する変異プロテアーゼバイオセンサー
US20160291000A1 (en) 2013-11-08 2016-10-06 Mcmaster University Methods of stabilizing molecules without refrigeration using water soluble polymers and applications thereof in performing chemical reactions

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Publication number Publication date
EP3526343A4 (en) 2020-08-05
WO2018071807A2 (en) 2018-04-19
EP3526343B1 (en) 2025-04-16
WO2018071807A3 (en) 2018-05-24
EP3526343A2 (en) 2019-08-21
US11261478B2 (en) 2022-03-01
US20190309342A1 (en) 2019-10-10
JP7615100B2 (ja) 2025-01-16
JP2023011608A (ja) 2023-01-24
JP2019537434A (ja) 2019-12-26

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