JP7127020B2 - ペプチド、タンパク質及び他の高分子の電気的検出方法 - Google Patents
ペプチド、タンパク質及び他の高分子の電気的検出方法 Download PDFInfo
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Description
Claims (14)
- 標本中に存在する少なくともアミノ酸1個が異なる一つ以上のペプチドまたはタンパク質を電気的に検出するためのアエロリジンナノポア又はナノチューブの使用であって、前記ナノポア又はナノチューブを脂質膜に挿入し、少なくとも2Mであり、かつ、6M未満の濃度のアルカリ金属ハロゲン化物電解質溶液を含む反応媒体中で40℃未満の温度にて-160mV超の電位差を前記膜に与え、前記ペプチドまたはタンパク質をその長さとその質量により識別することを目的とする前記使用。
- 前記アルカリ金属ハロゲン化物がアルカリ金属塩化物である請求項1に記載のナノポアの使用。
- 前記アルカリ金属ハロゲン化物がLiCl、KCl又はNaClに代表される請求項1又は2に記載のナノポアの使用。
- 前記電解質溶液の濃度が2M~5Mである請求項1~3のいずれか一項に記載のナノポアの使用。
- 前記反応媒体のLiCl濃度が1M又は4Mである請求項4に記載のナノポアの使用。
- 前記脂質膜に与える電位差が-80~-10mVである請求項1~5のいずれか一項に記載のナノポアの使用。
- 前記脂質膜に与える電位差が-29mVである請求項6に記載のナノポアの使用。
- 前記反応媒体の温度が3~33℃である請求項1~7のいずれか一項に記載のナノポアの使用。
- 電気的な検出が、ペプチド、タンパク質のサンプルの酵素分解の分解物を決定するために実施される、請求項1~8のいずれか一項に記載のナノポアの使用。
- 電気的な検出が、異なる配列のペプチド又はタンパク質を分離するために実施される、請求項1~8のいずれか一項に記載のナノポアの使用。
- 電気的な検出が、タンパク質またはペプチドの天然又は合成化学修飾を同定するために実施される、請求項1~8のいずれか一項に記載のナノポアの使用。
- 電気的な検出が、単一の原核細胞又は真核細胞中における酵素の酵素活性を定量するために実施される、請求項1~8のいずれか一項に記載のナノポアの使用。
- 電気的な検出が、代謝経路又は細胞内シグナル伝達経路からの代謝産物の分子を同定及び定量するために実施される、請求項1~8のいずれか一項に記載のナノポアの使用。
- 単一ペプチドが長さおよび質量により検出され、および識別される、請求項1~8のいずれか一項に記載のナノポアの使用。
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FR1601007 | 2016-06-24 | ||
FR1601007A FR3053119A1 (fr) | 2016-06-24 | 2016-06-24 | Procede de detection electrique de peptides, proteines et autres macromolecules |
PCT/FR2017/000129 WO2017220875A2 (fr) | 2016-06-24 | 2017-06-26 | Procede de detection electrique de peptides, proteines et autres macromolecules |
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US (1) | US11002657B2 (ja) |
EP (1) | EP3270139B9 (ja) |
JP (1) | JP7127020B2 (ja) |
FR (1) | FR3053119A1 (ja) |
WO (1) | WO2017220875A2 (ja) |
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CN109358106B (zh) * | 2018-11-05 | 2021-04-13 | 中国科学院重庆绿色智能技术研究院 | 一种基于固态纳米孔技术的多糖单分子结构解析方法 |
NL2023062B1 (en) * | 2019-05-03 | 2020-11-30 | Univ Delft Tech | Protein current trace signal acquisition using a nanopore |
CN111912892B (zh) * | 2019-05-07 | 2022-03-25 | 南京大学 | 气单胞菌溶素纳米孔通道在生物磷酸化及相关酶分析中的应用 |
CN111323469A (zh) * | 2020-02-14 | 2020-06-23 | 中国科学院重庆绿色智能技术研究院 | 一种基于纳米孔水解反应的免疫球蛋白m检测方法 |
CN112179955B (zh) * | 2020-09-27 | 2024-05-10 | 西北工业大学 | 基于聚精氨酸和聚谷氨酸逐层修饰的纳米孔制备pH响应的纳米流体二极管方法 |
DE102021200425A1 (de) | 2021-01-18 | 2022-07-21 | Albert-Ludwigs-Universität Freiburg, Körperschaft des öffentlichen Rechts | Verfahren und Systeme zur Identifikation einer Sequenz von Monomerbausteinen eines biologischen oder synthetischen Heteropolymers |
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US20150060276A1 (en) | 2012-03-13 | 2015-03-05 | Peking University | Nanopore Control With Pressure and Voltage |
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CN105368938A (zh) | 2015-11-06 | 2016-03-02 | 中国科学院重庆绿色智能技术研究院 | 一种基于电击穿在氮化硅薄膜上精确制备纳米孔的方法 |
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FR3053119A1 (fr) | 2017-12-29 |
EP3270139B9 (fr) | 2022-12-07 |
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EP3270139A3 (fr) | 2018-07-04 |
US20190317006A1 (en) | 2019-10-17 |
WO2017220875A3 (fr) | 2018-03-08 |
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EP3270139B1 (fr) | 2020-08-26 |
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