JP7124082B2 - ウナギのための嗜好性成分をスクリーニングする方法 - Google Patents
ウナギのための嗜好性成分をスクリーニングする方法 Download PDFInfo
- Publication number
- JP7124082B2 JP7124082B2 JP2020531317A JP2020531317A JP7124082B2 JP 7124082 B2 JP7124082 B2 JP 7124082B2 JP 2020531317 A JP2020531317 A JP 2020531317A JP 2020531317 A JP2020531317 A JP 2020531317A JP 7124082 B2 JP7124082 B2 JP 7124082B2
- Authority
- JP
- Japan
- Prior art keywords
- protein
- amino acid
- seq
- eel
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000019629 palatability Nutrition 0.000 title claims description 41
- 238000000034 method Methods 0.000 title claims description 37
- 238000012216 screening Methods 0.000 title claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 103
- 150000001413 amino acids Chemical group 0.000 claims description 88
- 102000004169 proteins and genes Human genes 0.000 claims description 75
- 239000002773 nucleotide Substances 0.000 claims description 54
- 125000003729 nucleotide group Chemical group 0.000 claims description 54
- 101000659774 Homo sapiens Taste receptor type 1 member 3 Proteins 0.000 claims description 33
- 102100035942 Taste receptor type 1 member 3 Human genes 0.000 claims description 33
- FFDGPVCHZBVARC-UHFFFAOYSA-N N,N-dimethylglycine Chemical compound CN(C)CC(O)=O FFDGPVCHZBVARC-UHFFFAOYSA-N 0.000 claims description 32
- 101000659765 Homo sapiens Taste receptor type 1 member 2 Proteins 0.000 claims description 31
- 150000008575 L-amino acids Chemical class 0.000 claims description 31
- 102100035948 Taste receptor type 1 member 2 Human genes 0.000 claims description 31
- 241000251468 Actinopterygii Species 0.000 claims description 28
- 241000252073 Anguilliformes Species 0.000 claims description 26
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 claims description 22
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims description 20
- 108091005708 gustatory receptors Proteins 0.000 claims description 19
- 239000000833 heterodimer Substances 0.000 claims description 17
- 239000000126 substance Substances 0.000 claims description 17
- 108700003601 dimethylglycine Proteins 0.000 claims description 16
- 229940078490 n,n-dimethylglycine Drugs 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 15
- 239000004615 ingredient Substances 0.000 claims description 15
- 241000252087 Anguilla japonica Species 0.000 claims description 11
- 229940109239 creatinine Drugs 0.000 claims description 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 10
- 108010077895 Sarcosine Proteins 0.000 claims description 10
- 229940043230 sarcosine Drugs 0.000 claims description 10
- 108091006099 G alpha subunit Proteins 0.000 claims description 8
- 102000034353 G alpha subunit Human genes 0.000 claims description 8
- 241000252082 Anguilla anguilla Species 0.000 claims description 7
- 210000004899 c-terminal region Anatomy 0.000 claims description 7
- 108010047357 Luminescent Proteins Proteins 0.000 claims description 6
- 102000006830 Luminescent Proteins Human genes 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 108020001507 fusion proteins Proteins 0.000 claims description 5
- 102000037865 fusion proteins Human genes 0.000 claims description 5
- 108010000239 Aequorin Proteins 0.000 claims description 4
- 241000008350 Anguilla interioris Species 0.000 claims description 4
- 230000001418 larval effect Effects 0.000 claims description 4
- 241000862519 Anguilla bicolor bicolor Species 0.000 claims description 3
- 241000862522 Anguilla bicolor pacifica Species 0.000 claims description 3
- 230000000366 juvenile effect Effects 0.000 claims description 3
- 235000018102 proteins Nutrition 0.000 description 53
- 235000001014 amino acid Nutrition 0.000 description 37
- 229940024606 amino acid Drugs 0.000 description 36
- 235000019688 fish Nutrition 0.000 description 22
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 18
- 108091006027 G proteins Proteins 0.000 description 17
- 102000030782 GTP binding Human genes 0.000 description 17
- 108091000058 GTP-Binding Proteins 0.000 description 17
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 16
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 15
- 229960003767 alanine Drugs 0.000 description 15
- 230000004044 response Effects 0.000 description 15
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 12
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 12
- 108020004414 DNA Proteins 0.000 description 11
- 102000005962 receptors Human genes 0.000 description 10
- 108020003175 receptors Proteins 0.000 description 10
- 229960001153 serine Drugs 0.000 description 10
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 9
- 238000007792 addition Methods 0.000 description 9
- 238000012217 deletion Methods 0.000 description 9
- 230000037430 deletion Effects 0.000 description 9
- 239000003446 ligand Substances 0.000 description 9
- 229960002429 proline Drugs 0.000 description 9
- 239000004471 Glycine Substances 0.000 description 8
- 101000659767 Homo sapiens Taste receptor type 1 member 1 Proteins 0.000 description 8
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 8
- 102100035941 Taste receptor type 1 member 1 Human genes 0.000 description 8
- 238000006467 substitution reaction Methods 0.000 description 8
- 229930182821 L-proline Natural products 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 229960002449 glycine Drugs 0.000 description 6
- 235000019640 taste Nutrition 0.000 description 6
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 5
- 241000251730 Chondrichthyes Species 0.000 description 5
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 5
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 5
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 5
- 241000124008 Mammalia Species 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 229960002885 histidine Drugs 0.000 description 5
- 235000019583 umami taste Nutrition 0.000 description 5
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 4
- 229930064664 L-arginine Natural products 0.000 description 4
- 235000014852 L-arginine Nutrition 0.000 description 4
- 125000000539 amino acid group Chemical group 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000252097 Anguilla australis Species 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 241000239366 Euphausiacea Species 0.000 description 3
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 235000004279 alanine Nutrition 0.000 description 3
- 229960001230 asparagine Drugs 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 2
- 241000252084 Anguilla Species 0.000 description 2
- 241000008344 Anguilla megastoma Species 0.000 description 2
- 241000212880 Anguilla reinhardtii Species 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 241000555825 Clupeidae Species 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 241000252099 Conger myriaster Species 0.000 description 2
- 108010062183 G protein alpha 16 Proteins 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 102100039215 Guanine nucleotide-binding protein G(t) subunit alpha-3 Human genes 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 241000252104 Muraenesocidae Species 0.000 description 2
- YHIPILPTUVMWQT-UHFFFAOYSA-N Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 description 2
- 241000276569 Oryzias latipes Species 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000269821 Scombridae Species 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 235000009697 arginine Nutrition 0.000 description 2
- 229960003121 arginine Drugs 0.000 description 2
- 239000012131 assay buffer Substances 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- -1 e.g. Substances 0.000 description 2
- 235000006694 eating habits Nutrition 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 108010005995 gustducin Proteins 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 235000014304 histidine Nutrition 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 235000018977 lysine Nutrition 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000019512 sardine Nutrition 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 229960002898 threonine Drugs 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 241001108251 Acontias breviceps Species 0.000 description 1
- 241000008339 Anguilla australis australis Species 0.000 description 1
- 241000008342 Anguilla australis schmidti Species 0.000 description 1
- 241000189613 Anguilla bengalensis bengalensis Species 0.000 description 1
- 241001477934 Anguilla bengalensis labiata Species 0.000 description 1
- 241000212875 Anguilla celebesensis Species 0.000 description 1
- 241001215078 Anguilla luzonensis Species 0.000 description 1
- 241001478121 Anguilla malgumora Species 0.000 description 1
- 241000252089 Anguilla marmorata Species 0.000 description 1
- 241000212876 Anguilla mossambica Species 0.000 description 1
- 241000008348 Anguilla nebulosa Species 0.000 description 1
- 241000008346 Anguilla obscura Species 0.000 description 1
- 241000252085 Anguilla rostrata Species 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 241000272878 Apodiformes Species 0.000 description 1
- 241000821071 Aquilegia nigricans Species 0.000 description 1
- 241001298657 Ariosoma meeki Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000131500 Chionoecetes opilio Species 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 241000252100 Conger Species 0.000 description 1
- 241000252095 Congridae Species 0.000 description 1
- 241000270722 Crocodylidae Species 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 241000111265 Gnathophis nystromi nystromi Species 0.000 description 1
- 241000994306 Gymnothorax kidako Species 0.000 description 1
- 241000396033 Gymnothorax ocellatus Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000522084 Leptocephalus Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 239000012097 Lipofectamine 2000 Substances 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 241000249085 Muraenesox bagio Species 0.000 description 1
- 241000252113 Muraenesox cinereus Species 0.000 description 1
- 241000204801 Muraenidae Species 0.000 description 1
- 101000904075 Mus musculus Guanine nucleotide-binding protein subunit alpha-15 Proteins 0.000 description 1
- BACYUWVYYTXETD-UHFFFAOYSA-N N-Lauroylsarcosine Chemical compound CCCCCCCCCCCC(=O)N(C)CC(O)=O BACYUWVYYTXETD-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001417523 Plesiopidae Species 0.000 description 1
- 241001274189 Pomatomus saltatrix Species 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000249080 Synaphobranchus kaupii Species 0.000 description 1
- 241001441722 Takifugu rubripes Species 0.000 description 1
- 102000045879 Taste receptor type 2 Human genes 0.000 description 1
- 108091005711 Taste receptors type 2 (bitter) Proteins 0.000 description 1
- 241001441724 Tetraodontidae Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 241001504592 Trachurus trachurus Species 0.000 description 1
- 108010087042 Transducin Proteins 0.000 description 1
- 102000006612 Transducin Human genes 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 108010041089 apoaequorin Proteins 0.000 description 1
- 239000008122 artificial sweetener Substances 0.000 description 1
- 235000021311 artificial sweeteners Nutrition 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 229960002433 cysteine Drugs 0.000 description 1
- LNNWVNGFPYWNQE-GMIGKAJZSA-N desomorphine Chemical compound C1C2=CC=C(O)C3=C2[C@]24CCN(C)[C@H]1[C@@H]2CCC[C@@H]4O3 LNNWVNGFPYWNQE-GMIGKAJZSA-N 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021191 food habits Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004545 gene duplication Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 230000001339 gustatory effect Effects 0.000 description 1
- 239000012676 herbal extract Substances 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 108091006093 heterotrimeric G proteins Proteins 0.000 description 1
- 102000034345 heterotrimeric G proteins Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960003136 leucine Drugs 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 229960003646 lysine Drugs 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229960004452 methionine Drugs 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 210000003370 receptor cell Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 230000030738 sensory perception of sweet taste Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000036327 taste response Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 235000019607 umami taste sensations Nutrition 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 229960004295 valine Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Animal Husbandry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2018134223 | 2018-07-17 | ||
JP2018134223 | 2018-07-17 | ||
PCT/JP2019/027943 WO2020017504A1 (ja) | 2018-07-17 | 2019-07-16 | ウナギのための嗜好性成分をスクリーニングする方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2020017504A1 JPWO2020017504A1 (ja) | 2021-08-19 |
JP7124082B2 true JP7124082B2 (ja) | 2022-08-23 |
Family
ID=69163836
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020531317A Active JP7124082B2 (ja) | 2018-07-17 | 2019-07-16 | ウナギのための嗜好性成分をスクリーニングする方法 |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP7124082B2 (zh) |
TW (1) | TWI735007B (zh) |
WO (1) | WO2020017504A1 (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023154597A1 (en) * | 2022-02-11 | 2023-08-17 | Firmenich Incorporated | Cells expressing umami taste receptors and uses thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001231461A (ja) | 2000-02-28 | 2001-08-28 | San Baiorekkusu:Kk | 魚介類用飼料添加剤およびこれを含む魚介類用飼料 |
JP2005500318A (ja) | 2001-06-26 | 2005-01-06 | セノミックス、インコーポレイテッド | T1rヘテロオリゴマー味覚受容体及び前記受容体を発現する細胞系並びに味覚化合物の特定のためのその使用 |
JP2006087348A (ja) | 2004-09-24 | 2006-04-06 | Nishinippon Environmental Energy Co Inc | 水産養殖用飼料 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09121783A (ja) * | 1995-09-01 | 1997-05-13 | Res Inst For Prod Dev | 魚介類用餌料 |
-
2019
- 2019-07-16 JP JP2020531317A patent/JP7124082B2/ja active Active
- 2019-07-16 WO PCT/JP2019/027943 patent/WO2020017504A1/ja active Application Filing
- 2019-07-17 TW TW108125278A patent/TWI735007B/zh active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001231461A (ja) | 2000-02-28 | 2001-08-28 | San Baiorekkusu:Kk | 魚介類用飼料添加剤およびこれを含む魚介類用飼料 |
JP2005500318A (ja) | 2001-06-26 | 2005-01-06 | セノミックス、インコーポレイテッド | T1rヘテロオリゴマー味覚受容体及び前記受容体を発現する細胞系並びに味覚化合物の特定のためのその使用 |
JP2006087348A (ja) | 2004-09-24 | 2006-04-06 | Nishinippon Environmental Energy Co Inc | 水産養殖用飼料 |
Non-Patent Citations (3)
Title |
---|
Yasuka TODA et al.,Establishment of a new Cell-based assay to measure the activity of sweeteners in fluorescent food ex,journal of agricultural and food chemistry,2011年11月23日,vol.59,No.22,PP.12131-12138 |
山下敦子,X線小角散乱法による味覚受容体リガンド結合ドメインの味物質結合に伴う構造変化の解析,分子研レターズ,vol.75,2017年03月,PP.28-29 |
石丸喜朗,食品成分の受容・伝達と生体応答の分子基盤,三島海雲記念財団年次報告書,2017年11月,PP.18-21 |
Also Published As
Publication number | Publication date |
---|---|
TWI735007B (zh) | 2021-08-01 |
TW202014516A (zh) | 2020-04-16 |
WO2020017504A1 (ja) | 2020-01-23 |
JPWO2020017504A1 (ja) | 2021-08-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Nollet et al. | Handbook of seafood and seafood products analysis | |
US7909001B2 (en) | Methods of raising crustaceans in low salinity water | |
Zhang et al. | AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain | |
JP7124082B2 (ja) | ウナギのための嗜好性成分をスクリーニングする方法 | |
Tang et al. | TANK-binding kinase-1 broadly affects oyster immune response to bacteria and viruses | |
Xu et al. | Identification and expression analysis of a doublesex1 gene in Daphnia pulex during different reproductive stages | |
JP2019074379A (ja) | 生ニンニク様香料素材のスクリーニング方法 | |
US11237155B2 (en) | Screening methods using olfactory receptors and novel compounds identified using the same | |
Wang et al. | A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure | |
US10393740B2 (en) | Methods for identifying modulators of calcium-sensing receptors | |
Murata et al. | Compositions of extractive components in the testes and ovaries of various sea urchins: comparisons among species, sexes, and maturational status | |
Tsutsui et al. | The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus | |
Jang et al. | Dim-light photoreceptor of chub mackerel Scomber japonicus and the photoresponse upon illumination with LEDs of different wavelengths | |
Hayakawa et al. | Precursor structure of egg proteins in the coral Galaxea fascicularis | |
US20220120734A1 (en) | Screening methods using canine t2r receptors and pet food products and compositions identified using the same | |
CN102575279A (zh) | 咸味调节物质的筛选方法 | |
AU2017250779B2 (en) | Methods for identifying modulators of GPR92 | |
JP6319504B2 (ja) | 塩味修飾物質のスクリーニング方法 | |
JP2010516232A (ja) | キメラうま味受容体、それをコードする核酸およびその利用 | |
Kim et al. | The influence of water temperature on the induction of vitellogenin in walleye pollock Gadus chalcogrammus | |
Cui et al. | Expression of gastrin and cholecystokinin B receptor in Lateolabrax maculatus | |
TW554044B (en) | Production of biologically active recombinant insulin-like growth factor II polypeptides | |
Stempel | Comparative Analyses of Murine and Human Formyl Peptide Receptor 3 | |
EP3807638A1 (en) | Screening methods using gprc6a taste receptors and pet food products and compositions prepared using the same | |
Lindemann | Receptor seeks ligand |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20211112 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20220617 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20220720 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20220801 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20220810 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7124082 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |