JP7104710B2 - ＴＣＲαホーミングエンドヌクレアーゼバリアント - Google Patents

ＴＣＲαホーミングエンドヌクレアーゼバリアント Download PDF

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JP7104710B2
JP7104710B2 JP2019540313A JP2019540313A JP7104710B2 JP 7104710 B2 JP7104710 B2 JP 7104710B2 JP 2019540313 A JP2019540313 A JP 2019540313A JP 2019540313 A JP2019540313 A JP 2019540313A JP 7104710 B2 JP7104710 B2 JP 7104710B2
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polypeptide
seq
cells
variant
amino acid
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JP2019532674A5 (enExample
JP2019532674A (ja
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ジョーダン ジャージョル，
マーク ポグソン，
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２セブンティ バイオ インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Television Signal Processing For Recording (AREA)
  • Treating Waste Gases (AREA)
  • Peptides Or Proteins (AREA)
JP2019540313A 2016-10-11 2017-10-11 ＴＣＲαホーミングエンドヌクレアーゼバリアント Active JP7104710B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662406689P 2016-10-11 2016-10-11
US62/406,689 2016-10-11
US201662414266P 2016-10-28 2016-10-28
US62/414,266 2016-10-28
PCT/US2017/056178 WO2018071565A1 (en) 2016-10-11 2017-10-11 TCRa HOMING ENDONUCLEASE VARIANTS

Publications (3)

Publication Number Publication Date
JP2019532674A JP2019532674A (ja) 2019-11-14
JP2019532674A5 JP2019532674A5 (enExample) 2020-11-19
JP7104710B2 true JP7104710B2 (ja) 2022-07-21

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JP2019540313A Active JP7104710B2 (ja) 2016-10-11 2017-10-11 ＴＣＲαホーミングエンドヌクレアーゼバリアント

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Country Link
US (2) US11591582B2 (enExample)
EP (1) EP3534916A4 (enExample)
JP (1) JP7104710B2 (enExample)
KR (1) KR20190065382A (enExample)
CN (1) CN110036107B (enExample)
AU (1) AU2017342273B2 (enExample)
CA (1) CA3039812A1 (enExample)
IL (1) IL265895A (enExample)
MA (1) MA46717A (enExample)
MX (1) MX2019004156A (enExample)
RU (1) RU2019114035A (enExample)
SG (1) SG11201903230QA (enExample)
WO (1) WO2018071565A1 (enExample)

Families Citing this family (10)

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Publication number Priority date Publication date Assignee Title
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CA3035455A1 (en) 2016-09-08 2018-03-15 Bluebird Bio, Inc. Pd-1 homing endonuclease variants, compositions, and methods of use
MX2019004156A (es) 2016-10-11 2019-09-26 Bluebird Bio Inc Variantes de endonucleasa homing de receptor de linfocitos t alfa (tcralpha).
AU2017346683B2 (en) 2016-10-17 2024-04-18 Regeneron Pharmaceuticals, Inc. TGFβR2 endonuclease variants, compositions, and methods of use
US12404500B2 (en) 2018-12-10 2025-09-02 Novo Nordisk A/S Homing endonuclease variants
JP2024503249A (ja) * 2020-12-21 2024-01-25 ２セブンティ バイオ インコーポレイテッド 部位特異的変異導入のための組成物及び方法
WO2022266038A1 (en) 2021-06-14 2022-12-22 2Seventy Bio, Inc. Single stranded rna purification methods
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20130315884A1 (en) 2012-05-25 2013-11-28 Roman Galetto Methods for engineering allogeneic and immunosuppressive resistant t cell for immunotherapy
US20140208457A1 (en) 2011-06-10 2014-07-24 Basf Plant Science Company Gmbh Nuclease Fusion Protein and Uses Thereof
JP2016520320A (ja) 2013-05-31 2016-07-14 セレクティスＣｅｌｌｅｃｔｉｓ Ｔ細胞受容体α遺伝子を開裂するＬＡＧＬＩＤＡＤＧホーミングエンドヌクレアーゼおよびその用途

Family Cites Families (10)

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US20020119158A1 (en) * 1998-12-17 2002-08-29 Corixa Corporation Compositions and methods for the therapy and diagnosis of ovarian cancer
US7329731B2 (en) * 2001-08-31 2008-02-12 Medigene Limited Soluble T cell receptor
KR100415267B1 (ko) * 2002-02-19 2004-01-16 오광수 2단 발효법에 의한 고 순도 멸치액젓 및 그 제조방법
EP1413626A1 (en) * 2002-10-23 2004-04-28 Vicuron Pharmaceuticals, Inc. Genes and proteins for the biosynthesis of the glycopeptide antibiotic A40926
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US8956828B2 (en) 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
US20140148361A1 (en) 2010-06-07 2014-05-29 Barry L. Stoddard Generation and Expression of Engineered I-ONUI Endonuclease and Its Homologues and Uses Thereof
US9034813B2 (en) * 2010-09-17 2015-05-19 Ecolab Usa Inc. High performance low viscoelasticity foaming detergent compositions employing extended chain anionic surfactants
US8450107B1 (en) 2011-11-30 2013-05-28 The Broad Institute Inc. Nucleotide-specific recognition sequences for designer TAL effectors
MX2019004156A (es) 2016-10-11 2019-09-26 Bluebird Bio Inc Variantes de endonucleasa homing de receptor de linfocitos t alfa (tcralpha).

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20140208457A1 (en) 2011-06-10 2014-07-24 Basf Plant Science Company Gmbh Nuclease Fusion Protein and Uses Thereof
US20130315884A1 (en) 2012-05-25 2013-11-28 Roman Galetto Methods for engineering allogeneic and immunosuppressive resistant t cell for immunotherapy
JP2016520320A (ja) 2013-05-31 2016-07-14 セレクティスＣｅｌｌｅｃｔｉｓ Ｔ細胞受容体α遺伝子を開裂するＬＡＧＬＩＤＡＤＧホーミングエンドヌクレアーゼおよびその用途

Non-Patent Citations (1)

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BOISSEL S. et al.，Nucleic Acid Research，2014年，Vol.42 No.4，p.2591-2601

Also Published As

Publication number Publication date
JP2019532674A (ja) 2019-11-14
RU2019114035A (ru) 2020-11-13
KR20190065382A (ko) 2019-06-11
IL265895A (en) 2019-06-30
EP3534916A1 (en) 2019-09-11
US20220186198A1 (en) 2022-06-16
EP3534916A4 (en) 2020-09-30
SG11201903230QA (en) 2019-05-30
MA46717A (fr) 2019-09-11
WO2018071565A1 (en) 2018-04-19
MX2019004156A (es) 2019-09-26
US12264343B2 (en) 2025-04-01
US11591582B2 (en) 2023-02-28
CN110036107B (zh) 2024-12-27
CN110036107A (zh) 2019-07-19
AU2017342273B2 (en) 2023-07-06
US20230357736A1 (en) 2023-11-09
AU2017342273A1 (en) 2019-05-09
CA3039812A1 (en) 2018-04-19

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