JP7084667B2 - 誘電泳動を用いたマイクロ電極バイオセンサ、及びこれを用いた生体物質検出方法 - Google Patents
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Description
を備える
Z=R+jX=R+j(XL-XC)=R-jXC=R-j(1/wC)...(1)
図11に示すように、マイクロパターンの構造によるバイオセンサの性能を確認するために、3つの構造のマイクロパターンをデザインした。
図14は、抗体固定化による表面処理方法の模式図を示し、表面処理後に抗体が固定化されたバイオセンサの表面を示すものである。同図に示すように、各マイクロ電極の間に「-OH」結合基が活性化されたSiO2表面に自己組織化単分子層を形成するために、気相(Vapour Phase)のAPMEMSを用いており、自己組織化単分子層を形成した後、抗体を固定するためのリンカー(Linker)として「EDC/NHs」を使用した。
マイクロパターンを有するバイオセンサの性能を評価するために、表面活性化条件を適用し、誘電泳動力の効果を確認することをまず実行しなければならないので、誘電泳動効果が現れる最適の条件を確立し、これを用いて定量分析する順序で実験を行った。
確立された誘電泳動電圧条件を用いてアミロイドβタンパク質を定量分析し、100fg/mLから100pg/mLに濃度を10倍に増加させたアミロイドβサンプルを、1×PBSに溶解して準備した。
最適化された誘電泳動効果に基づくアミロイドβ定量分析センサを用いて、血漿中のアミロイドβの定量分析を実施し、ここに使用されたバイオセンサのタイプは、Type#3(線形マイクロパターンを用いたセンサ)である。
110 第1のマイクロ電極
120 第2のマイクロ電極
130 マイクロパターン
Claims (11)
- 複数の第1の突出電極が基板上に櫛(Comb)状に配列されている第1のマイクロ電極と;
前記第1のマイクロ電極の各第1の突出電極と交互に配置され、櫛状を有する複数の第2の突出電極が配列されている第2のマイクロ電極と;
前記第1のマイクロ電極と第2のマイクロ電極との間の空間に形成される導電性材質のマイクロパターンと;
前記第1のマイクロ電極と第2のマイクロ電極との間の空間に固定され、標的生体物質に特異的に反応する複数の受容体と;
を備える、誘電泳動を用いたマイクロ電極バイオセンサであって、
前記第1のマイクロ電極及び第2のマイクロ電極には、誘電泳動力を発生させるための交流電圧が印加され、
前記第1のマイクロ電極と第2のマイクロ電極との間で測定されたインピーダンス値に基づいて前記標的生体物質の存在有無および濃度の少なくとも一方を算出する、誘電泳動を用いたマイクロ電極バイオセンサ。。 - 前記マイクロパターンは、正方形、長方形、および線形のいずれかの形状に形成されることを特徴とする、請求項1に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 前記正方形の大きさは、各辺の長さが2.0μm~3.0μmであることを特徴とする、請求項2に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 前記長方形の大きさは、一方の辺の長さが2.0μm~3.0μmであり、他方の辺の長さが7.0μm~8.0μmであることを特徴とする、請求項2に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 前記線形マイクロパターンの幅は、2.0μm~3.0μmであることを特徴とする、請求項2に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 前記誘電泳動力を発生させるための交流電圧の大きさは0.25V~0.35Vであり、周波数は50MHzであることを特徴とする、請求項1に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 前記標的生体物質はアミロイドβタンパク質を含み、前記受容体はアミロイドβ抗体を含むことを特徴とする、請求項1に記載の誘電泳動を用いたマイクロ電極バイオセンサ。
- 請求項1に記載の誘電泳動を用いたマイクロ電極バイオセンサの第1のマイクロ電極及び第2のマイクロ電極に電圧を印加するステップと;
前記第1のマイクロ電極と第2のマイクロ電極との間のインピーダンスを測定するステップと;
前記測定されたインピーダンス値に基づいて標的生体物質の存在有無および濃度の少なくとも一方を算出するステップと;
を含む、バイオセンサを用いた生体物質検出方法であって、
前記第1のマイクロ電極及び第2のマイクロ電極に印加される電圧は、誘電泳動力を発生させるための交流電圧である、バイオセンサを用いた生体物質検出方法。 - 電圧の大きさが0.25V~0.35Vであり、周波数が50MHzであることを特徴とする、請求項8に記載のバイオセンサを用いた生体物質検出方法。
- 前記標的生体物質の重量は、4.0kDa~5.0kDaであることを特徴とする、請求項8に記載のバイオセンサを用いた生体物質検出方法。
- 前記標的生体物質は、アミロイドβタンパク質を含むことを特徴とする、請求項8に記載のバイオセンサを用いた生体物質検出方法。
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KR1020180080348A KR102102534B1 (ko) | 2018-07-11 | 2018-07-11 | 유전 전기 영동을 이용한 마이크로 전극 바이오 센서, 및 이를 이용한 생체물질 검출 방법 |
KR10-2018-0080348 | 2018-07-11 | ||
PCT/KR2019/008461 WO2020013590A1 (ko) | 2018-07-11 | 2019-07-10 | 유전 전기 영동을 이용한 마이크로 전극 바이오 센서, 및 이를 이용한 생체물질 검출 방법 |
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US20030157587A1 (en) | 2000-04-17 | 2003-08-21 | Rafael Gomez | Biosensor and related method |
JP2013257348A (ja) | 2008-04-15 | 2013-12-26 | Panasonic Corp | 微粒子測定装置および微粒子測定方法 |
US20140131204A1 (en) | 2012-11-13 | 2014-05-15 | Academia Sinica | Molecular entrapment and enrichment |
US20160209299A1 (en) | 2013-08-29 | 2016-07-21 | Apocell, Inc. | Method and apparatus for isolation, capture and molecular analysis of target particles |
WO2017142166A1 (ko) | 2016-02-19 | 2017-08-24 | 한국과학기술연구원 | 유전체 전기영동을 이용한 마이크로 전극 바이오 센서 |
JP2018009993A (ja) | 2012-01-27 | 2018-01-18 | ユニバーシティ オブ テネシー リサーチ ファウンデーション | 交流動電によるバイオマーカーの検出のための方法および装置 |
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US7911610B2 (en) * | 2005-07-20 | 2011-03-22 | Shimadzu Corporation | Optical measuring device |
EP1996734B1 (en) * | 2006-03-09 | 2018-08-01 | The Regents of The University of California | Method and apparatus for target detection using electrode-bound viruses |
CA2643354C (en) * | 2006-03-16 | 2016-04-26 | Al-Amin Dhirani | Dielectric sensing method and system |
US8313628B2 (en) * | 2006-07-19 | 2012-11-20 | Shimadzu Corporation | Method and apparatus for evaluating dielectrophoretic intensity of microparticle |
US9465003B2 (en) * | 2010-12-16 | 2016-10-11 | Korea Research Institute Of Bioscience And Biotechnology | Membrane phase electrode using printing and bio-molecule detection using same |
KR101551876B1 (ko) * | 2013-12-31 | 2015-09-09 | 서울대학교산학협력단 | 플로팅 전극을 이용한 탄소나노튜브 기반 바이오센서, 이의 제조방법 및 이를 이용하여 비부착성 세포의 전기생리학적 반응을 측정하는 방법 |
KR101648383B1 (ko) * | 2014-09-19 | 2016-08-24 | 한국과학기술연구원 | 교차전극 바이오센서 |
KR101709762B1 (ko) * | 2015-02-09 | 2017-02-23 | 광운대학교 산학협력단 | 생체분자 농축 기능 일체형 센서 및 그 제조방법 |
KR101912890B1 (ko) * | 2016-10-04 | 2018-10-29 | 한국과학기술연구원 | 표적 생체물질과 수용체의 반응을 개선한 교차 전극 바이오센서 |
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Publication number | Priority date | Publication date | Assignee | Title |
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US20030157587A1 (en) | 2000-04-17 | 2003-08-21 | Rafael Gomez | Biosensor and related method |
JP2013257348A (ja) | 2008-04-15 | 2013-12-26 | Panasonic Corp | 微粒子測定装置および微粒子測定方法 |
JP2018009993A (ja) | 2012-01-27 | 2018-01-18 | ユニバーシティ オブ テネシー リサーチ ファウンデーション | 交流動電によるバイオマーカーの検出のための方法および装置 |
US20140131204A1 (en) | 2012-11-13 | 2014-05-15 | Academia Sinica | Molecular entrapment and enrichment |
US20160209299A1 (en) | 2013-08-29 | 2016-07-21 | Apocell, Inc. | Method and apparatus for isolation, capture and molecular analysis of target particles |
WO2017142166A1 (ko) | 2016-02-19 | 2017-08-24 | 한국과학기술연구원 | 유전체 전기영동을 이용한 마이크로 전극 바이오 센서 |
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KR20200006681A (ko) | 2020-01-21 |
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