JP7046097B2 - 試料核酸にアダプターを付着する方法 - Google Patents

試料核酸にアダプターを付着する方法 Download PDF

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JP7046097B2
JP7046097B2 JP2019555645A JP2019555645A JP7046097B2 JP 7046097 B2 JP7046097 B2 JP 7046097B2 JP 2019555645 A JP2019555645 A JP 2019555645A JP 2019555645 A JP2019555645 A JP 2019555645A JP 7046097 B2 JP7046097 B2 JP 7046097B2
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adapter
sample
stranded
nucleic acid
double
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JP2020516281A5 (https=
JP2020516281A (ja
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アンドリュー ケネディ，
ステファニー アン ウォード モーティマー，
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ガーダント ヘルス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2019555645A 2017-04-14 2018-04-13 試料核酸にアダプターを付着する方法 Active JP7046097B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022016339A JP7514263B2 (ja) 2017-04-14 2022-02-04 試料核酸にアダプターを付着する方法

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762485769P 2017-04-14 2017-04-14
PCT/US2017/027809 WO2017181146A1 (en) 2016-04-14 2017-04-14 Methods for early detection of cancer
USPCT/US2017/027809 2017-04-14
US62/485,769 2017-04-14
US201762486663P 2017-04-18 2017-04-18
US62/486,663 2017-04-18
US201762517145P 2017-06-08 2017-06-08
US62/517,145 2017-06-08
PCT/US2018/027632 WO2018191702A2 (en) 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids

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JP2022016339A Division JP7514263B2 (ja) 2017-04-14 2022-02-04 試料核酸にアダプターを付着する方法

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JP2020516281A JP2020516281A (ja) 2020-06-11
JP2020516281A5 JP2020516281A5 (https=) 2021-05-20
JP7046097B2 true JP7046097B2 (ja) 2022-04-01

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JP2022016339A Active JP7514263B2 (ja) 2017-04-14 2022-02-04 試料核酸にアダプターを付着する方法

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US (2) US20200131567A1 (https=)
EP (2) EP3885445B1 (https=)
JP (2) JP7046097B2 (https=)
CN (1) CN110546272B (https=)
AU (1) AU2018252018A1 (https=)
CA (1) CA3057163A1 (https=)
ES (2) ES2962223T3 (https=)
HU (1) HUE063675T2 (https=)
PL (1) PL3885445T3 (https=)
WO (1) WO2018191702A2 (https=)

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CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
JP6913089B2 (ja) 2015-11-11 2021-08-04 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
ES3002715T3 (en) 2016-04-14 2025-03-07 Guardant Health Inc Methods for early detection of cancer
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
CN116144735A (zh) * 2019-08-12 2023-05-23 深圳市真迈生物科技有限公司 核酸样本处理方法、测序方法和试剂盒
US11891653B2 (en) * 2019-09-30 2024-02-06 Guardant Health, Inc. Compositions and methods for analyzing cell-free DNA in methylation partitioning assays
EP4048812B1 (en) * 2019-10-25 2023-12-06 Guardant Health, Inc. Methods for 3' overhang repair
WO2022055984A1 (en) * 2020-09-08 2022-03-17 Resolution Bioscience, Inc. Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
EP4251760A1 (en) * 2020-11-25 2023-10-04 Alida Biosciences, Inc. Multiplexed profiling of rna and dna modifications
KR20230121076A (ko) * 2020-12-15 2023-08-17 제노다이브 파마 가부시키가이샤 Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법
EP4388091A2 (en) * 2021-08-20 2024-06-26 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
AU2022397404A1 (en) 2021-11-24 2024-06-13 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
AU2024259004A1 (en) * 2023-04-21 2025-12-04 Twist Bioscience Corporation Polymerase variants

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JP2016535979A (ja) 2013-09-25 2016-11-24 サーモ フィッシャー サイエンティフィック バルチックス ユーエービー Ｄｎａ末端修復、アデニル化、リン酸化のための酵素組成物

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US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2585593B1 (en) * 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
US8829172B2 (en) * 2011-03-11 2014-09-09 Academia Sinica Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification
DK3089822T3 (da) * 2013-12-30 2022-05-02 Atreca Inc Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder
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JP2016535979A (ja) 2013-09-25 2016-11-24 サーモ フィッシャー サイエンティフィック バルチックス ユーエービー Ｄｎａ末端修復、アデニル化、リン酸化のための酵素組成物

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
AU2018252018A1 (en) 2019-10-10
EP3885445C0 (en) 2023-08-23
ES2868074T3 (es) 2021-10-21
PL3885445T3 (pl) 2024-01-29
CA3057163A1 (en) 2018-10-18
EP3610032A2 (en) 2020-02-19
JP2022048389A (ja) 2022-03-25
CN110546272A (zh) 2019-12-06
US20200283839A1 (en) 2020-09-10
ES2962223T3 (es) 2024-03-18
WO2018191702A3 (en) 2019-02-21
JP2020516281A (ja) 2020-06-11
CN110546272B (zh) 2024-06-07
EP3610032A4 (en) 2020-05-06
EP3610032B1 (en) 2021-03-10
JP7514263B2 (ja) 2024-07-10
EP3885445A1 (en) 2021-09-29
EP3885445B1 (en) 2023-08-23
US20200131567A1 (en) 2020-04-30
HUE063675T2 (hu) 2024-01-28
WO2018191702A2 (en) 2018-10-18

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