CN110546272B - 将衔接子附接至样品核酸的方法 - Google Patents
将衔接子附接至样品核酸的方法 Download PDFInfo
- Publication number
- CN110546272B CN110546272B CN201880025117.1A CN201880025117A CN110546272B CN 110546272 B CN110546272 B CN 110546272B CN 201880025117 A CN201880025117 A CN 201880025117A CN 110546272 B CN110546272 B CN 110546272B
- Authority
- CN
- China
- Prior art keywords
- sample
- adaptors
- nucleic acid
- stranded
- polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/107—Probe or oligonucleotide ligation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762485769P | 2017-04-14 | 2017-04-14 | |
| USPCT/US2017/027809 | 2017-04-14 | ||
| US62/485,769 | 2017-04-14 | ||
| PCT/US2017/027809 WO2017181146A1 (en) | 2016-04-14 | 2017-04-14 | Methods for early detection of cancer |
| US201762486663P | 2017-04-18 | 2017-04-18 | |
| US62/486,663 | 2017-04-18 | ||
| US201762517145P | 2017-06-08 | 2017-06-08 | |
| US62/517,145 | 2017-06-08 | ||
| PCT/US2018/027632 WO2018191702A2 (en) | 2017-04-14 | 2018-04-13 | Methods of attaching adapters to sample nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110546272A CN110546272A (zh) | 2019-12-06 |
| CN110546272B true CN110546272B (zh) | 2024-06-07 |
Family
ID=63792821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880025117.1A Active CN110546272B (zh) | 2017-04-14 | 2018-04-13 | 将衔接子附接至样品核酸的方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20200131567A1 (https=) |
| EP (2) | EP3610032B1 (https=) |
| JP (2) | JP7046097B2 (https=) |
| CN (1) | CN110546272B (https=) |
| AU (1) | AU2018252018A1 (https=) |
| CA (1) | CA3057163A1 (https=) |
| ES (2) | ES2868074T3 (https=) |
| HU (1) | HUE063675T2 (https=) |
| PL (1) | PL3885445T3 (https=) |
| WO (1) | WO2018191702A2 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4293125A3 (en) | 2012-12-10 | 2024-02-28 | Resolution Bioscience, Inc. | Methods for targeted genomic analysis |
| US20160053301A1 (en) | 2014-08-22 | 2016-02-25 | Clearfork Bioscience, Inc. | Methods for quantitative genetic analysis of cell free dna |
| RU2018121254A (ru) | 2015-11-11 | 2019-12-16 | Резолюшн Байосайенс, Инк. | Высокоэффективное построение библиотек днк |
| EP4488686A3 (en) | 2016-04-14 | 2025-04-30 | Guardant Health, Inc. | Methods for early detection of cancer |
| US11319594B2 (en) | 2016-08-25 | 2022-05-03 | Resolution Bioscience, Inc. | Methods for the detection of genomic copy changes in DNA samples |
| CN116144735A (zh) * | 2019-08-12 | 2023-05-23 | 深圳市真迈生物科技有限公司 | 核酸样本处理方法、测序方法和试剂盒 |
| US11891653B2 (en) * | 2019-09-30 | 2024-02-06 | Guardant Health, Inc. | Compositions and methods for analyzing cell-free DNA in methylation partitioning assays |
| US11447819B2 (en) * | 2019-10-25 | 2022-09-20 | Guardant Health, Inc. | Methods for 3′ overhang repair |
| WO2022055984A1 (en) * | 2020-09-08 | 2022-03-17 | Resolution Bioscience, Inc. | Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis |
| JP2023551072A (ja) | 2020-11-25 | 2023-12-06 | アリダ・バイオサイエンシーズ・インコーポレイテッド | Ｒｎａおよびｄｎａ修飾の多重プロファイリング |
| JPWO2022131285A1 (https=) * | 2020-12-15 | 2022-06-23 | ||
| EP4388091A2 (en) * | 2021-08-20 | 2024-06-26 | Guardant Health, Inc. | Methods for simultaneous molecular and sample barcoding |
| WO2023097295A1 (en) | 2021-11-24 | 2023-06-01 | Alida Biosciences, Inc. | Rna and dna analysis using engineered surfaces |
| KR20250175336A (ko) * | 2023-04-21 | 2025-12-16 | 트위스트 바이오사이언스 코포레이션 | 중합효소 변이체 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105722978A (zh) * | 2013-09-25 | 2016-06-29 | 赛默飞世尔科技波罗的海有限公司 | 用于dna末端修复、腺苷酸化、磷酸化的酶组合物 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US20030017081A1 (en) | 1994-02-10 | 2003-01-23 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| AU2002359522A1 (en) | 2001-11-28 | 2003-06-10 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| WO2011161549A2 (en) * | 2010-06-24 | 2011-12-29 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
| US8829172B2 (en) | 2011-03-11 | 2014-09-09 | Academia Sinica | Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification |
| CA2935122C (en) | 2013-12-30 | 2023-09-19 | Atreca, Inc. | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| CA2956925C (en) * | 2014-08-01 | 2024-02-13 | Dovetail Genomics, Llc | Tagging nucleic acids for sequence assembly |
| WO2016135300A1 (en) * | 2015-02-26 | 2016-09-01 | Qiagen Gmbh | Efficiency improving methods for gene library generation |
-
2018
- 2018-04-13 ES ES18783709T patent/ES2868074T3/es active Active
- 2018-04-13 CA CA3057163A patent/CA3057163A1/en active Pending
- 2018-04-13 HU HUE21157081A patent/HUE063675T2/hu unknown
- 2018-04-13 EP EP18783709.1A patent/EP3610032B1/en active Active
- 2018-04-13 CN CN201880025117.1A patent/CN110546272B/zh active Active
- 2018-04-13 JP JP2019555645A patent/JP7046097B2/ja active Active
- 2018-04-13 PL PL21157081.7T patent/PL3885445T3/pl unknown
- 2018-04-13 AU AU2018252018A patent/AU2018252018A1/en not_active Abandoned
- 2018-04-13 EP EP21157081.7A patent/EP3885445B1/en active Active
- 2018-04-13 ES ES21157081T patent/ES2962223T3/es active Active
- 2018-04-13 WO PCT/US2018/027632 patent/WO2018191702A2/en not_active Ceased
-
2019
- 2019-10-08 US US16/596,180 patent/US20200131567A1/en not_active Abandoned
-
2020
- 2020-05-21 US US16/880,706 patent/US20200283839A1/en not_active Abandoned
-
2022
- 2022-02-04 JP JP2022016339A patent/JP7514263B2/ja active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105722978A (zh) * | 2013-09-25 | 2016-06-29 | 赛默飞世尔科技波罗的海有限公司 | 用于dna末端修复、腺苷酸化、磷酸化的酶组合物 |
Non-Patent Citations (1)
| Title |
|---|
| Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase;Yoshiyuki Ohtsubo等;Scientific Reports;第7卷;摘要和第5页最后一段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3610032B1 (en) | 2021-03-10 |
| CN110546272A (zh) | 2019-12-06 |
| EP3885445A1 (en) | 2021-09-29 |
| EP3610032A4 (en) | 2020-05-06 |
| US20200131567A1 (en) | 2020-04-30 |
| PL3885445T3 (pl) | 2024-01-29 |
| US20200283839A1 (en) | 2020-09-10 |
| JP2020516281A (ja) | 2020-06-11 |
| JP7514263B2 (ja) | 2024-07-10 |
| ES2962223T3 (es) | 2024-03-18 |
| WO2018191702A2 (en) | 2018-10-18 |
| HUE063675T2 (hu) | 2024-01-28 |
| EP3610032A2 (en) | 2020-02-19 |
| JP2022048389A (ja) | 2022-03-25 |
| WO2018191702A3 (en) | 2019-02-21 |
| EP3885445B1 (en) | 2023-08-23 |
| JP7046097B2 (ja) | 2022-04-01 |
| AU2018252018A1 (en) | 2019-10-10 |
| CA3057163A1 (en) | 2018-10-18 |
| ES2868074T3 (es) | 2021-10-21 |
| EP3885445C0 (en) | 2023-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110546272B (zh) | 将衔接子附接至样品核酸的方法 | |
| EP3177740B1 (en) | Digital measurements from targeted sequencing | |
| CN109844137B (zh) | 用于鉴定嵌合产物的条形码化环状文库构建 | |
| CN105408496A (zh) | 检测稀有突变和拷贝数变异的系统和方法 | |
| CN113227393B (zh) | 用于校准表观遗传分区测定的方法、组合物和系统 | |
| US12534757B2 (en) | Methods for 3′ overhang repair | |
| US20240141425A1 (en) | Correcting for deamination-induced sequence errors | |
| JP2026067857A (ja) | ３’オーバーハングを修復する方法 | |
| JP2026510027A (ja) | ハイブリッドｓｓＤＮＡ－およびｄｓＤＮＡ－ＮＧＳライブラリー調製方法 | |
| HK40027193B (zh) | 具有可变长度非随机独特分子标识符的通用短衔接子 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |