JP6957289B2 - Whitening cosmetic composition - Google Patents

Description

The present invention relates to a whitening cosmetic composition.

As a whitening ingredient contained in whitening cosmetics, a stearyldimethylbenzylammonium chloride-hydroquinone molecular complex (“stearylconium chloride (hereinafter, may be referred to as“ SHQ ”)) (Patent Document 1) is known.

On the other hand, a lactic acid-glycolic acid copolymer (hereinafter sometimes referred to as "PLGA") is a substance known to form nanoparticles capable of conjugating a substance inside, and a drug is placed inside the PLGA nanoparticles. A drug delivery system (DDS) that conjugates and delivers to a required place in the body is known (Patent Documents 2 to 4). It is also known that fullerene nanoparticles are used for DDS (Patent Document 5). Further, platinum colloid particles (also referred to as "platinum nanocolloids") are known to have an antioxidant effect, and are also used as additives in cosmetic compositions (Patent Document 6).

Japanese Unexamined Patent Publication No. 2004-99542 Japanese Unexamined Patent Publication No. 2006-28031 Japanese Unexamined Patent Publication No. 2006-131577 Japanese Unexamined Patent Publication No. 2011-529492 Japanese Patent Application Laid-Open No. 2005-281231 Japanese Unexamined Patent Publication No. 2012-031086

An object of the present invention is to provide a novel whitening cosmetic composition having an excellent whitening effect.

As a result of diligent research, the inventors of the present application have found that a cosmetic containing nanoparticles such as PLGA and SHQ exerts a remarkable whitening effect, and completed the present invention.

That is, the present invention is a whitening cosmetic composition containing nanoparticles and a stearyldimethylbenzylammonium chloride-hydroquinone molecular complex , wherein the nanoparticles are lactic acid-glycolic acid copolymer nanoparticles. The composition is provided.

INDUSTRIAL APPLICABILITY According to the present invention, a novel whitening cosmetic composition exhibiting an excellent whitening effect has been provided.

As the nanoparticles contained as essential components in the whitening cosmetic composition of the present invention, PLGA nanoparticles, fullerene nanoparticles and platinum colloidal particles are preferable. Of these, PLGA nanoparticles are particularly preferable from the viewpoint of whitening effect. Since these nanoparticles themselves and their production methods are known and commercially available, commercially available products can be used. PLGA and fullerenes are used in DDS because they can form nanoparticles and conjugate desired substances within them. The particle size of the nanoparticles is not particularly limited, and the average particle size is usually about 10 nm to 1000 nm, particularly about 50 nm to 400 nm, but is not limited thereto. The blending amount of the nanoparticles is not particularly limited, but is usually about 0.001% by mass to 30% by mass, preferably about 0.05% by mass to 5.0% by mass, and more preferably 0.2% by mass to 1.0% by mass with respect to the total amount of the composition. It is about%.

SHQ itself contained as an essential ingredient in the whitening cosmetic composition of the present invention and a method for producing the same are known, and are also known as whitening ingredients contained in cosmetics. Since SHQ is also commercially available, a commercially available product can be used. The blending amount of SHQ is not particularly limited, but is usually about 1 part by mass to 10000 parts by mass, preferably about 10 parts by mass to 1000 parts by mass, and more preferably 100 parts by mass to 400 parts by mass with respect to 100 parts by mass of nanoparticles. It is about a part.

The cosmetic composition of the present invention may contain each of the above components in a solvent, and water is preferable as the solvent. When the solvent is water, it is in the form of a lotion. In the case of lotion, the cosmetic of the present invention can be produced by putting each component in water and homogenizing it. Further, the cosmetic composition of the present invention is not limited to the form of a lotion, and may be in the form of a cream or gel by a well-known method. In the form of cream, as is well known, additives such as glyceryl monostearate, polyethylene glycol monostearate, polysorbate, polyoxyethylene hydrogenated castor oil, and polyoxyethylene lauryl ethers are added, for example, from 0.10% by mass to 20.00% by mass. It can be made into a cream form by adding about%. In the case of gel form, as is well known, carboxyvinyl polymer, (acrylic acid / alkyl acrylate) crosspolymer, polyacrylamide, gelatin, hydroxyethyl cellulose, xanthan gum, lysine dilauroyl glutamate Na, acrylicyrrolid crosspolymer, bentonites, etc. , Hectrites, (Acrylate / steareth itaconate-20) copolymer, hydroxypropyl starch phosphate, Na polyglutamate, polyvinylpyrrolidone, (vinylpyrrolidone / VA) copolymer, stearoxyhydroxypropylmethylcellulose, polyvinyl alcohol, white jellyfish polysaccharide, etc. The gel can be formed by adding, for example, about 0.01% by mass to 10.00% by mass of the additive of.

The cosmetic composition of the present invention may further contain various additives used in cosmetics in addition to the above-mentioned essential ingredients. Examples of such additives include, but are not limited to, antioxidants, antibacterial agents, moisturizers, anti-inflammatory agents, surfactants and the like. Examples of the antioxidant include ascorbic acid and its derivatives, placenta, hydroquinone and its derivatives, tranexamic acid, kojic acid, tocopherol, lemon juice and the like. Examples of the antibacterial agent include parabens, phenoxyethanol, pentylene glycol, octanediol and the like. Examples of the moisturizer include glycerin, butylene glycol (BG), sodium hyaluronate, ceramides, chondroitin sulfate Na, collagen, citrus leaf extract, Jiaogulan leaf extract, and getto leaf extract. Examples of the anti-inflammatory agent include dipotassium glycyrrhizinate, allantoin, ginseng extract, ginseng extract, chamomile extract, licorice flavonoid and the like. Examples of the surfactant include PEG hydrogenated castor oil and the like.

Hereinafter, the present invention will be specifically described based on examples. However, the present invention is not limited to the following examples.

Examples 1 to 3, Comparative Examples 1 and 2
1. 1. Preparation of cosmetic composition A lotion having the composition shown in Table 1 below was prepared. Each component shown in Table 1 was weighed by the amount shown in Table 1, and purified water was added to make the total amount 100 g. At this time, an appropriate amount of purified water was added and then homogenized, and purified water was further added to bring the total amount to 100 g. Purified water was used as a control. Commercially available products were used as each component. The average particle size of PLGA was 88 nm, the average particle size of fullerenes was 300 nm, and the average particle size of platinum colloidal particles was 250 nm.

＊グリコール溶液：濃グリセリン10g + 1,3-BG 10g + PEG-60水添ヒマシ油 2g

* Glycol solution: Concentrated glycerin 10g + 1,3-BG 10g + PEG-60 Hydrogenated castor oil 2g

2. Whitening effect test To evaluate the whitening effect, use a human three-dimensional cultured epidermis model containing melanocytes (trade name: Labsite Melano Model, manufactured by Japan Tissue Engineering), and measure the amount of melanin produced according to the instruction manual. I went by. Specifically, it was carried out as follows.

To the melanocyte-containing human three-dimensional cultured epidermis model, the compositions of each of the above examples were added in the amounts shown in Table 2 and cultured for 14 days. 0.05 mL each of positive and negative controls was added. The cultured three-dimensional cultured epidermis was collected in a microtube, and 150 μL of a buffer solution (1% SDS, 0.05 mmol / L EDTA, 10 mmol / L Tris-HCl) was added and immersed. To this, 3 μL of proteinase K (5 mg / mL) was added and treated at 45 ° C. for 16 hours, and further 3 μL of proteinase K (5 mg / mL) was added and treated at 45 ° C. for 4 hours. After the above treatment, 25 μL of 30% hydrogen peroxide solution containing 500 mmol / L sodium carbonate was added, and the mixture was reacted at 80 ° C. for 30 minutes. The reaction solution was centrifuged (15,000 rpm, 10 minutes), and the supernatant was collected. With respect to the supernatant, the absorbance at a wavelength of 405 nm (Abs. 405 nm) and the absorbance at a wavelength of 570 nm (Abs. 570 nm) were measured, and Abs. From 405 nm to Abs. 570 nm was subtracted. Then, the amount of melanin per well was calculated from the calibration curve prepared separately. In addition, the relative value of the amount of melanin produced was determined with the amount of melanin produced in the negative control as 100%. The same experiment was performed three times, and the average value was taken. The results are shown in Table 2 below.

Amount of melanin (%) = [Bs-Bb / Bn-Bb] x 100
Bs: Absorbance of the evaluation substance (405nm-570nm)
Bn: Absorbance of positive control (vitamin E :) (405nm-570nm)
Bb: Negative subject (blank): Purified water (IPA alone) Absorbance

We also confirmed the survival rate of melanocytes. This was specifically determined using the MTT (3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide) reagent and according to the instructions above. The relative value was calculated with the number of living cells of the negative control as 100%. As a result, it was confirmed that the survival rate was 97% or more in each case and each addition amount, and that almost no cell death occurred.

As shown in Table 2, especially when 0.5 mL of the composition of the present invention was added, the amount of melanin produced was reduced to 41% to 51%, as is best shown in the result of the addition amount of 0.5 mL. On the other hand, in Comparative Example 1 containing only SHQ without nanoparticles and Comparative Example 2 containing only ascorbic acid, which is a known whitening component, the amount of melanin produced was reduced to only 77% or 84%. However, a remarkable whitening effect of the composition of the present invention was confirmed. In addition, the whitening effect was highest when the nanoparticles were PLGA nanoparticles.

Claims (1)

A whitening cosmetic composition comprising nanoparticles and a stearyldimethylbenzylammonium chloride-hydroquinone molecular complex , wherein the nanoparticles are lactic acid-glycolic acid copolymer nanoparticles .