JP6936536B1 - Bactericidal / virus-inactivating composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To exhibit an excellent bactericidal effect and an excellent virus inactivating effect, for example, when a lower alcohol is used at a low concentration or not blended, while sufficiently preventing an influence on the appearance of a specific object to be washed. To provide a bactericidal / virus inactivating composition exhibiting an excellent bactericidal effect and an excellent virus inactivating effect. A bactericidal / virus-inactivating composition comprising an amine compound having an acid dissociation constant (pKa) of 9.0 or more and a cationic surfactant. [Selection diagram] None

Description

The present invention relates to a bactericidal / virus-inactivating composition.

Bacteria such as Staphylococcus aureus and Salmonella are known as causative bacteria that cause food poisoning. In order to prevent food poisoning caused by these bacteria, it has been conventionally practiced to kill these bacteria using a bactericidal composition such as a lower alcohol or a quaternary ammonium compound.

In recent years, infectious gastroenteritis or food poisoning caused by viruses such as norovirus has frequently occurred throughout the year, and the peak of the outbreak is from 11 to March. In particular, norovirus is an RNA virus having no envelope (hereinafter referred to as "norovirus, etc.") classified into Caliciviridae and Norovirus genus, and is alcohol (ethanol, isopropanol, etc.), heat, acid (stomach acid, etc.), Alternatively, it has a strong resistance to drying and the like. The incubation period is thought to be 1 to 2 days, and the main symptoms of nausea, vomiting, and diarrhea appear, but may be accompanied by abdominal pain, headache, fever, chills, muscle pain, sore throat, and malaise.

Furthermore, a new type of coronavirus infection has occurred in 2020. To prevent splash infection, for example, at supermarkets, department stores, restaurants, accommodation facilities, medical facilities, offices, etc., acrylic plates and plastic curtains are installed at checkout counters, reception counters, interview counters, counters, etc. There is an increasing number of people installing acrylic partitions on tables such as counter seats in restaurants and on desks in offices.

As a composition for preventing food poisoning caused by bacteria and viruses, Patent Document 1 describes 40 to 95 wt% lower alcohol, 0.01 to 5 wt% alkaline substance, and 0.01 to 10 wt% cationic surfactant. The composition containing the above is disclosed.

Japanese Unexamined Patent Publication No. 2008-189645

Under the current circumstances as described above, a composition exhibiting an excellent bactericidal effect and virus inactivating effect is required. Here, the cationic surfactant can exert a bactericidal effect and an enveloped virus inactivating effect, but does not have a non-enveloped virus inactivating effect by itself. Sodium hypochlorite, povidone iodine, and some alcohol preparations are the only effective means to inactivate non-enveloped viruses. Increasing the variation of the composition suitably used for such sterilization / virus inactivating applications can broaden the range of choices when the composition is used in various aspects in the application, and is a great technical technique. it makes sense.
In addition, sodium hypochlorite has a strong oxidizing power and may have an unfavorable effect on the object to be used (object to be washed) such as metal and fiber. Povidone iodine can also have an unfavorable effect on metal objects to be washed. Further, the composition described in Cited Document 1 contains a high concentration of lower alcohol as an essential component together with an alkaline substance and a cationic surfactant. When such an alcohol preparation is used for an object to be washed made of plastic, for example, the object to be washed may be damaged and the appearance may be impaired.

The present invention has been made in view of the above-mentioned current situation, and an object of the present invention is to exhibit an excellent bactericidal effect and an excellent virus inactivating effect, for example, when a lower alcohol is set to a low concentration or not blended. Is to provide a bactericidal / virus-inactivating composition that exhibits an excellent bactericidal effect and an excellent virus-inactivating effect while sufficiently preventing the influence on the appearance of a specific object to be washed.

That is, the bactericidal / virus inactivating composition of the present invention is characterized by containing an amine compound having an acid dissociation constant (pKa) of 9.0 or more and a cationic surfactant.

In addition, in this specification, a "bactericidal / virus inactivating composition" means sterilizing the bacterium or inactivating the virus against at least one bacterium and / or at least one virus. It means a composition used for sterilization. For example, when the composition is used for disinfecting acrylic plates or plastic curtains to prevent droplet infection, or toilet bowls, toilet seats, floors, walls, etc., the composition is sterilized and virus-inactivated. It can be said that it is used as a composition.

The bactericidal / virus inactivating composition of the present invention contains an amine compound having an acid dissociation constant (pKa) of 9.0 or more. When such an amine compound is combined with a cationic surfactant, the bactericidal effect and virus inactivating effect of the cationic surfactant can be improved. As a result, the bactericidal / virus inactivating composition of the present invention exhibits an excellent bactericidal effect and an excellent virus inactivating effect. As described above, the cationic surfactant can exert a bactericidal effect and an enveloped virus inactivating effect, but does not have a non-enveloped virus inactivating effect by itself. On the other hand, the bactericidal / virus inactivating composition of the present invention exhibits a high virus inactivating effect on norovirus, which is a non-enveloped virus, due to the synergistic effect of a specific amine compound and a cationic surfactant. be.
Examples of the "amine compound" include primary amines, secondary amines, and tertiary amines. The pKa of the amine compound in the present specification means the pKa of the amino group of the amine compound in water at 25 ° C. The amino group is a monovalent functional group in which hydrogen is removed from ammonia, a primary amine or a secondary amine.
An amine compound having an acid dissociation constant (pKa) of 9.0 or more means that when the amine compound has a plurality of amino groups and there are a plurality of pKas, the highest pKa can be used as a reference, which is the highest. The pKa may be 9.0 or more, but it is preferable that the pKa of all the amino groups in the amine compound is 9.0 or more.

In the bactericidal / virus inactivating composition of the present invention, the above amine compounds are aminoethanol, aminopropanol, aminobutanol, methylethanolamine, ethylethanolamine, butylethanolamine, methylpropanolamine, ethylpropanolamine, dimethylaminoethanol. , Diethylaminoethanol, ethylmethylaminoethanol, dimethylaminopropanol, diethylaminopropanol, aminomethylpropanol, aminoethylpropanol, aminopropylpropanol, aminobutylpropanol, ethylenediamine, diethylenetriamine, triethylenetetramine, piperidine, pyrrolidine, piperazine, benzylamine, amino It is preferably at least one selected from the group consisting of methylbenzylamine, aminoethylbenzylamine, and aminoethoxybenzylamine.
These amine compounds are highly volatile and the components are unlikely to remain after use. As a result, the problem that the amine compound stays on the surface of the object to be washed such as an acrylic plate or a toilet seat for a long time and damages the object to be washed and spoils the appearance of the object to be washed is sufficiently prevented. can. In addition, the amine compound stays on the surface of the object to be washed for a long time, easily touches the human skin, and can sufficiently prevent problems such as roughening of the human skin. Furthermore, these amine compounds can further enhance the bactericidal and virus-inactivating effects of the bactericidal / virus-inactivating composition of the present invention.

In the sterilizing / virus-inactivating composition of the present invention, the amine compound preferably has a vapor pressure of 50 Pa or more.
When the vapor pressure of the amine compound is 50 Pa or more, the volatility is high, so that the amine compound is unlikely to remain on the surface of the object to be washed such as an acrylic plate and a toilet seat after use. Thereby, as described above, the influence on the appearance of the object to be washed can be further prevented. Moreover, even if it adheres to human skin, it is considered that it is unlikely to damage human skin. For example, when the bactericidal / virus inactivating composition of the present invention is used for a toilet bowl or toilet seat, it is possible to prevent cracks in the toilet seat or the like, prevent an influence on the appearance, and make a book on the toilet bowl or toilet seat. Even if the bactericidal / virus inactivating composition of the present invention remains, and then the bactericidal / virus inactivating composition of the present invention adheres to the skin of the person using the toilet bowl, it is difficult to damage the person's skin. ..
The vapor pressure of the amine compound in the present specification means the vapor pressure at 20 ° C.
Further, in the present specification, the “vapor pressure” means a value according to the Antoine equation.

In the bactericidal / virus-inactivating composition of the present invention, the cationic surfactant is preferably a quaternary ammonium compound.
Since the cationic surfactant is a quaternary ammonium compound, the bactericidal effect and the virus inactivating effect of the bactericidal / virus inactivating composition of the present invention can be further enhanced.

In the bactericidal / virus inactivating composition of the present invention, the cationic surfactant is tetraalkylammonium salt, didecylmethylpolyoxyethylammonium salt, alkylbenzyldimethylammonium salt, cetylpyridinium salt, benzethonium salt, alkyldimethylhydroxy. It preferably contains at least one selected from the group consisting of ethylammonium salts and polyhexamethylene biguanidine hydrochloride.
The counter ion of the cationic surfactant is not particularly limited, and is, for example, chloride ion (chloride ion), methyl sulfate ion (methosulfate ion), carbonate ion (propionate ion), adipate ion (adipate ion). And so on.
The alkyl groups in the tetraalkylammonium salt may be the same or different. Examples of the difference include didecyldimethylammonium salt, dioctyldimethylammonium salt, didecyldimethylammonium salt, octyldecyldimethylammonium salt and the like.
These cationic surfactants can exhibit a more excellent bactericidal effect and an excellent virus inactivating effect in the bactericidal / virus inactivating composition of the present invention.

The total concentration of the anionic surfactant, the nonionic surfactant, and the zwitterionic surfactant in the sterilizing / virus inactivating composition of the present invention is preferably 2% by mass or less.
As a result, the bactericidal effect and the virus inactivating effect of the present invention become more remarkable.
The total concentration of the anionic surfactant, the nonionic surfactant, and the amphoteric ion surfactant is 2% by mass or less. It does not contain any of a surfactant and an amphoteric ionic surfactant, and the total concentration of the anionic surfactant, the nonionic surfactant and the amphoteric ionic surfactant may be 0% by mass.

In the bactericidal / virus-inactivating composition of the present invention, the pH is preferably 9.0 to 13.0.
The bactericidal / virus inactivating composition of the present invention can exhibit an excellent bactericidal effect and an excellent virus inactivating effect in a weakly alkaline range.
In the present specification, the pH is a value measured using a pH meter F-53 (manufactured by HORIBA, Ltd.) at 25 ° C. by a method according to JIS Z-8802: 2011 “pH measurement method”.
The pH can be adjusted by controlling the amount of additives such as an acid agent and an alkaline agent.

In the sterilizing / virus-inactivating composition of the present invention, the concentration of the lower alcohol is preferably 20% by mass or less.
When the concentration of lower alcohol is 20% by mass or less, the effect on the resin is particularly small, and the object to be used, such as the object to be washed made of plastic, the toilet bowl, toilet seat, floor, wall, etc. is damaged. Therefore, it is possible to sufficiently prevent the appearance and other characteristics from being impaired.
In the present specification, the lower alcohol refers to an alcohol having 5 or less carbon atoms. The valence of alcohol is not particularly limited.
The concentration of the lower alcohol is the total concentration of the plurality of lower alcohols when a plurality of lower alcohols are used.
The lower alcohol concentration of 20% by mass or less means that the sterilizing / virus-inactivating composition of the present invention does not contain the lower alcohol and the lower alcohol concentration may be 0% by mass. ..

The bactericidal / virus inactivating composition of the present invention is preferably a norovirus inactivating composition.
The bactericidal / virus inactivating composition of the present invention exhibits a high virus inactivating effect on norovirus.
As used herein, the term "norovirus inactivating composition" refers to a virus inactivating composition used for at least one virus selected from the group consisting of feline calicivirus, murine norovirus and human norovirus. means.
The bactericidal / virus inactivating composition of the present invention also exhibits a high virus inactivating effect against influenza virus and coronavirus, which have lower drug resistance than norovirus.

The bactericidal / virus-inactivating composition of the present invention is preferably used on the surface of a hard material.
The hard material is not particularly limited, and examples thereof include resins such as metals, ceramics, and plastics, glass, and surface-treated wood.

The present invention is also a hygienic material comprising the bactericidal / virus-inactivating composition of the present invention.
The hygienic material of the present invention is characterized by containing the bactericidal / virus-inactivating composition of the present invention.
Since the bactericidal / virus inactivating composition of the present invention can sufficiently exert a bactericidal effect and a virus inactivating effect, bacterial infection can be obtained by using a sanitary material containing such a bactericidal / virus inactivating composition. And virus infection can be sufficiently prevented.

The bactericidal / virus-inactivating composition of the present invention exhibits an excellent bactericidal effect and an excellent virus-inactivating effect. It exhibits an excellent bactericidal effect and an excellent virus inactivating effect while sufficiently preventing the influence on the appearance.

Hereinafter, the bactericidal / virus-inactivating composition of the present invention will be described while showing specific embodiments. However, the present invention is not limited to the following embodiments, and can be appropriately modified and applied without changing the gist of the present invention.

The sterilizing / virus inactivating composition of the present invention is characterized by containing an amine compound having an acid dissociation constant (pKa) of 9.0 or more and a cationic surfactant.
Each composition of the bactericidal / virus-inactivating composition of the present invention will be described below.

(Amine compound)
In the bactericidal / virus-inactivating composition of the present invention, the pKa of the amine compound is 9.0 or more. The pKa of the amine compound is preferably 9.15 or more, and more preferably 9.3 or more.
When the pKa of the amine compound is 9.0 or more, the amine compound can improve the bactericidal effect and the virus inactivating effect of the cationic surfactant by being combined with the cationic surfactant.
The upper limit of the pKa of the amine compound is not particularly limited, but is usually 14.0 or less.

In the sterilizing / virus-inactivating composition of the present invention, the amine compound preferably has a vapor pressure of 50 Pa or more, more preferably 60 Pa or more, further preferably 90 Pa or more, and 100 Pa or more. Is particularly preferred.
When the vapor pressure of the amine compound is 50 Pa or more, the volatility is high, so that the components are unlikely to remain after use.
The upper limit of the vapor pressure of the amine compound is not particularly limited, but is usually 50,000 Pa or less.

In the bactericidal / virus inactivating composition of the present invention, the amine compound is aminoethanol, aminopropanol, aminobutanol, methylethanolamine, ethylethanolamine, butylethanolamine, methylpropanolamine, ethylpropanolamine, dimethylaminoethanol, Diethylaminoethanol, ethylmethylaminoethanol, dimethylaminopropanol, diethylaminopropanol, aminomethylpropanol, aminoethylpropanol, aminopropylpropanol, aminobutylpropanol, ethylenediamine, diethylenetriamine, triethylenetetramine, piperidine, pyrrolidine, piperazine, benzylamine, aminomethyl It is preferably at least one selected from the group consisting of benzylamine, aminoethylbenzylamine, and aminoethoxybenzylamine.
The sterilizing / virus-inactivating composition of the present invention may contain only one kind of amine compound, or may contain two or more kinds of amine compounds.

In the bactericidal / virus-inactivating composition of the present invention, the concentration of the amine compound is preferably 0.2% by mass or more, more preferably 0.3% by mass or more, and 0.5% by mass or more. It is more preferably 0.8% by mass or more, and particularly preferably 1% by mass or more.
Further, in the sterilizing / virus-inactivating composition of the present invention, the concentration of the amine compound is preferably 10% by mass or less, and more preferably 5% by mass or less.
When the concentration of the amine compound is 0.2% by mass or more, the bactericidal effect and the virus inactivating effect become more excellent.
When the concentration of the amine compound is 10% by mass or less, the amine compound is less likely to remain on the object to be washed after the use of the sterilizing / virus-inactivating composition of the present invention.

(Cationic surfactant)
The bactericidal / virus inactivating composition of the present invention contains a cationic surfactant.
Sterilization and virus inactivation can be performed by contacting the cationic surfactant with bacteria or with a virus.
In particular, since the cationic surfactant exhibits a bactericidal effect even in a small amount, the bactericidal effect is unlikely to decrease even if the bactericidal / virus inactivating composition of the present invention is diluted with water in the environment.

In the bactericidal / virus inactivating composition of the present invention, the cationic surfactant is not particularly limited, and examples thereof include amine compounds and quaternary ammonium compounds, with quaternary ammonium compounds being preferable.

Further, in the bactericidal / virus inactivating composition of the present invention, the cationic surfactant is didecyldimethylammonium chloride, didecyldimethylammonium metosulfate, didecylmethylpolyoxyethylammonium propionate, dioctyldimethylammonium chloride, alkyl. From benzyldimethylammonium chloride, cetylpyridinium chloride, benzethonium chloride, alkyldimethylhydroxyethylammonium chloride, tetraalkylammonium adibinate, polyhexamethylenebiguanidine hydrochloride, didecyldimethylammonium carbonate, and octyldecyldimethylammonium chloride. It is preferable to include at least one selected from the group.
Among these cationic surfactants, those which are quaternary ammonium compounds are more preferable, and in particular, didecyldimethylammonium metosulfate, didecylmethylpolyoxyethylammonium propionate, alkylbenzyldimethylammonium chloride, and dioctyldimethylammonium chloride. , Alkyldimethylhydroxyethylammonium chloride and octyldecyldimethylammonium chloride are more preferred.
In the bactericidal / virus inactivating composition of the present invention, these cationic surfactants may be contained alone or a plurality of these cationic surfactants may be contained.

In the bactericidal / virus-inactivating composition of the present invention, the concentration of the cationic surfactant is preferably 0.006% by mass or more, more preferably 0.01% by mass or more, and 0.03% by mass. It is more preferably% or more, and particularly preferably 0.05% by mass or more.
When the concentration of the cationic surfactant is as high as this, the virus inactivating effect of the cationic surfactant can be made more excellent.
The concentration of the cationic surfactant is preferably 2% by mass or less, more preferably 1.4% by mass or less, further preferably 1.2% by mass or less, and 1% by mass or less. It is more preferable that there is, and it is particularly preferable that it is 0.9% by mass or less.
The low concentration of the cationic surfactant also makes the virus inactivating effect more excellent.

(Other ingredients)
In addition to the above components, the bactericidal / virus inactivating composition of the present invention contains surfactants, chelating agents, lower alcohols, other solvents, solubilizers, thickeners, enzymes, fragrances, pigment dyes and the like. It may be included.

The surfactant is not particularly limited as long as it is a surfactant other than the cationic surfactant, and an anionic surfactant, a nonionic surfactant, an amphoteric surfactant and the like can be used.
Examples of the nonionic surfactant include polyoxyalkylene alkyl ether, pluronic block polymer, reverse pluronic block polymer, tetronic block polymer, reverse tetronic block polymer, polyoxyalkylene alkyl phenyl ether, and fatty acid alkanol. Amid, polyoxyalkylene fatty acid alkanolamide, polyoxyalkylene fatty acid ester, polyoxyalkylene sorbitan fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, alkyl polyglycoside, polyoxyethylene methyl ether fatty acid ester, polyoxy Examples thereof include alkylene alkylamines.
Anionic surfactants include fatty acid salts, alkyl ether carboxylates, alkane sulfonates, α-olefin sulfonates, α-sulfomethyl ester salts, alkylbenzene sulfonates, alkyl succinates, alkyl sulfates, Examples thereof include alkyl ether sulfate ester salts.
Examples of the amphoteric surfactant include alkyl betaine, alkyl amino betaine, alkyl amide propyl betaine, alkyl hydroxy sulfo betaine, and alkyl amine oxide.

As described above, the total concentration of the anionic surfactant, the nonionic surfactant, and the zwitterionic surfactant in the sterilizing / virus inactivating composition of the present invention is preferably 2% by mass or less.
The total concentration of the anionic surfactant, the nonionic surfactant, and the zwitterionic surfactant is more preferably 1.2% by mass or less, and further preferably 1% by mass or less.

The chelating agent is not particularly limited, but is limited to trisodium nitrilotriacetic acid, tetrasodium ethylenediamine tetraacetate, disodium ethylenediamine tetraacetate, sodium methylglycine diacetate, sodium ethylenediaminetetramethylenephosphonate, sodium phosphonobutanetricarboxylic acid, and citric acid. Examples include sodium and the like.
The bactericidal / virus-inactivating composition of the present invention may contain only one type of these chelating agents, or may contain a plurality of types.
When the bactericidal / virus inactivating composition of the present invention contains a chelating agent, the mass concentration of the chelating agent in the bactericidal / virus inactivating composition of the present invention is preferably 0.01 to 5% by mass. , 0.05 to 2% by mass, more preferably.

The sterilizing / virus-inactivating composition of the present invention may contain a lower alcohol, but from the viewpoint of further reducing the influence on the object to be washed made of plastic, as described above, the sterilizing / virus-inactivating composition of the present invention. The concentration of the lower alcohol in the virus-inactivating composition is preferably 20% by mass or less.
The concentration of the lower alcohol is more preferably 10% by mass or less, further preferably 5% by mass or less, particularly preferably 1% by mass or less, and most preferably 0% by mass.
The bactericidal / virus inactivating composition of the present invention can exhibit an excellent bactericidal effect and an excellent virus inactivating effect even when the lower alcohol is low in concentration or not blended. be.

Further, in the sterilizing / virus inactivating composition of the present invention, the concentration of alcohol containing lower alcohol and higher alcohol is preferably 20% by mass or less.
The concentration of the alcohol is more preferably 10% by mass or less, further preferably 5% by mass or less, particularly preferably 1% by mass or less, and most preferably 0% by mass.
With such a low concentration of alcohol, it is possible to more sufficiently prevent the object to be washed made of plastic from being damaged and impairing its appearance and other characteristics.
In the present specification, alcohol refers to alcohol having an unlimited number of carbon atoms. The valence of alcohol is also not particularly limited.
The alcohol concentration is the total concentration of the plurality of alcohols when a plurality of alcohols are used.
The alcohol concentration of 20% by mass or less means that the sterilizing / virus-inactivating composition of the present invention does not contain alcohol and the alcohol concentration may be 0% by mass.
It does not contain col and may have an alcohol concentration of 0% by mass.

Further, the concentration of the solvent (organic solvent) in the sterilizing / virus-inactivating composition of the present invention is preferably less than 20% by mass.
The concentration of the solvent is more preferably 10% by mass or less, further preferably 5% by mass or less, particularly preferably 1% by mass or less, and most preferably 0% by mass.
With such a low concentration of the solvent, it is possible to more sufficiently prevent the object to be washed made of plastic from being damaged and impairing its appearance and other characteristics.
In the present specification, the solvent refers to an organic solvent.
The solvent concentration is the total concentration of the plurality of types of solvents when a plurality of types of solvents are used.
The solvent concentration of less than 20% by mass means that the sterilizing / virus-inactivating composition of the present invention does not contain the solvent and the solvent concentration may be 0% by mass.

The bactericidal / virus-inactivating composition of the present invention preferably further contains water.
Water is blended as a residue other than other components, and its content is not particularly limited. The water is not particularly limited, and examples thereof include tap water, distilled water, purified water, pure water, ion-exchanged water, and the like, and one or more of these can be used.
In the sterilizing / virus-inactivating composition of the present invention, the mass concentration of water is preferably 10.00 to 99.90% by mass, more preferably 40.00 to 99.80% by mass. It is more preferably 70.00 to 99.60% by mass, and particularly preferably 90.00 to 99.50% by mass.

The sterilizing / virus-inactivating composition of the present invention preferably has a pH of 9.0 to 13.0.
The pH is more preferably 10.0 or higher.
At such a pH, the virus inactivating effect is improved.
When the pH of the sterilizing / virus-inactivating composition is 13.0 or less, it is possible to further prevent rough hands and deterioration of the object to be washed.
As described above, the pH is adjusted by controlling the amount of an acid agent such as sulfuric acid, hydrochloric acid, or sulfamic acid, or an alkaline agent such as sodium hydroxide, potassium hydroxide, sodium silicate, or potassium silicate. can do.

Next, the use of the bactericidal / virus-inactivating composition of the present invention will be described.
As described above, the bactericidal / virus inactivating composition of the present invention is preferably a norovirus inactivating composition.
Above all, the bactericidal / virus inactivating composition of the present invention is preferably a human norovirus inactivating composition. The bactericidal / virus inactivating composition of the present invention can exert an inactivating effect on both feline calicivirus and murine norovirus, as shown in Examples described later, and inactivates human norovirus. It is applicable to.
Thus, in order to claim the effect on human norovirus, it is desirable that the inactivating effect on both feline calicivirus and murine norovirus has been demonstrated.
The bactericidal / virus inactivating composition of the present invention also exhibits a high virus inactivating effect against influenza virus and coronavirus, which have lower drug resistance than norovirus.

The bactericidal / virus-inactivating composition of the present invention can be used on the surface of a hard material or a soft material, but as described above, it is preferably used on the surface of a hard material. As shown in Examples described later, the bactericidal / virus inactivating composition of the present invention can exert a bactericidal effect and a virus inactivating effect with a contact time (action time) of only 30 seconds, and has a hard surface. It is applicable to cleaning and disinfection of viruses.
As described above, when cleaning and disinfecting a hard surface, the contact time needs to be 30 seconds. As described above, examples of the hard material include resins such as metals, ceramics and plastics, glass, and surface-treated wood. For example, a plastic plate such as an acrylic plate, a resin toilet bowl, a toilet seat, or the like can be mentioned as particularly suitable ones.
For example, the bactericidal / virus-inactivating composition of the present invention is preferably used for toilets.
In particular, toilet seats and the like are prone to cracking due to detergents, and a composition having little effect on the resin, such as the sterilizing / virus-inactivating composition of the present invention, is suitable.
In addition, in this specification, "used for a toilet" means to be used for the toilet bowl, the toilet seat, the floor, the wall, and the like existing in the toilet.

Further, the bactericidal / virus-inactivating composition of the present invention may be used as a sanitary material.
Since the bactericidal / virus inactivating composition of the present invention exhibits an excellent bactericidal effect and an excellent virus inactivating effect, by using a sanitary material containing such a bactericidal / virus inactivating composition, It can sufficiently prevent bacterial and viral infections.

The sanitary material is not particularly limited, and examples thereof include masks, disposable gloves, disposable cloths, tissue paper, and wet tissues.

The sterilizing / virus-inactivating composition of the present invention may be added to a hand-washing liquid, a neutral detergent, a deodorant, or the like.
Even if the bactericidal / virus inactivating composition of the present invention, the hand washing liquid containing the bactericidal / virus inactivating composition of the present invention, a neutral detergent, a deodorant, etc. are packed in a pump bottle or a spray bottle. good.

The bactericidal / virus inactivating composition of the present invention exhibits an excellent bactericidal effect and an excellent virus inactivating effect.
Further, in the sterilizing / virus-inactivating composition of the present invention, when the lower alcohol concentration is low (for example, 20% by mass or less), the object to be washed is made of a resin such as plastic or is coated with a resin. If it is a thing, it is hard to damage the object to be washed. As a matter of course, when the object to be washed is metal, ceramic, glass, surface-treated wood or the like, the object to be washed is not easily damaged regardless of the lower alcohol concentration or the like. Moreover, even if it adheres to human skin, it does not easily damage human skin.
Further, in the sterilizing / virus-inactivating composition of the present invention, when the vapor pressure of the amine compound is 50 Pa or more, the volatility is high, so that the components are unlikely to remain after use. Therefore, when the sterilizing / virus inactivating composition of the present invention is used, a part of the sterilizing / virus inactivating composition of the present invention remains in the object to be washed, and then the object to be washed is touched. Even if a part of the bactericidal / virus-inactivating composition of the present invention adheres to the skin of a person, the skin of the person is not easily damaged.

Examples of the present invention will be described in more detail below, but the present invention is not limited to these examples. In the examples, "%" means "mass%" unless otherwise specified.

( Reference Example 1, Examples 2 to 7, Reference Example 2, Examples 9 and 10, Reference Examples 3 and 4, Examples 13 to 17, Reference Example 5 and Examples 19 to 25) and (Comparative Examples 1 to 14) )
Reference Example 1, Examples 2 to 7, Reference Example 2, Examples 9 and 10, Reference Examples 3 and 4, Examples 13 to 17, Reference Example 5 and Example 19 to the formulations shown in Tables 1 to 3. The bactericidal / virus-inactivating composition according to 25 and Comparative Examples 1 to 14 was prepared.
In Tables 1 to 3, the manufacturers of the compounds and the like are as follows.
(Cationic surfactant)
Benzyldodecyldimethylammonium chloride: manufactured by Tokyo Kasei Kogyo Co., Ltd. benzyldimethylalkylammonium chloride didecyldimethylammonium metosulfate: manufactured by Lion Specialty Chemicals Co., Ltd. Lipocard 210-80MSPG Didecyldimethylammonium metosulfate dioctyldimethylammonium chloride: Ronza Japan Co., Ltd. Verdac LF-80 Dioctyldimethylammonium Chloride Alkyldimethylhydroxyethylammonium Chloride: Clariant Japan Co., Ltd. PRAEPAGEN HY Alkyl (C12-14) Didecylethylammonium Chloride Didecylmethylpolyoxyethylammonium Propionate : Ronza Japan Co., Ltd. Bardap26 Didecylmethylpoly (1-2) Oxyethylammonium Propionate

(Other surfactants)
Alkylamine Oxide: Made by Lion Specialty Chemicals Co., Ltd. Cadenax DM12D-W Lauryldimethylamine Oxide Polyoxyethylene Alkyl Ether: Made by Aoki Oil & Fat Industry Co., Ltd. Finesurf TD-90 Polyoxyethylene (9) Tridecyl ether Polyoxy Alkylene alkyl ether: Neugen LP-100 polyoxyalkylene lauryl ether alkyl glycoside manufactured by Daiichi Kogyo Seiyaku Co., Ltd. Betain: Clariant Japan Co., Ltd. Genagen CAB 818J Palm oil fatty acid amide propyl betaine alkyl alkanolamide: Kao Co., Ltd. Aminone C11-S Palm oil fatty acid N-methylethanolamide

(Chelating agent)
Trisodium methylglycine diacetate: Trilon M manufactured by BASF Limited
Ethylenediaminetetraacetic acid tetrasodium: Trilon BX Powder manufactured by BASF Limited
Sodium citrate: Trisodium citrate dihydrate manufactured by Hayashi Junyaku Kogyo Co., Ltd.

(Other ingredients)
72% dilute sulfuric acid: Toagosei Co., Ltd. Ethyl alcohol: Fujifilm Wako Pure Chemical Industries, Ltd. Ethanol (99.5)
Isopropyl alcohol: manufactured by Fujifilm Wako Pure Chemical Industries, Ltd. 2-propanol n-propyl alcohol: manufactured by Fujifilm Wako Pure Chemical Industries, Ltd. 1-propanol potassium hydroxide: manufactured by Fujifilm Wako Pure Chemical Industries, Ltd. Tripotassium phosphate: Wako Pure Chemical Industries, Ltd. Sodium hydrogen carbonate: Wako Pure Chemical Industries, Ltd. The compounds listed as amine compounds in Tables 1 to 3 are manufactured by Tokyo Kasei Kogyo Co., Ltd. The numerical values of the compositions in Tables 1 to 3 are the ratio (mass%) of the pure content of each component in the sterilizing / virus-inactivating composition.

<Volatility test>
Four sheets of Pro Wipe Soft Micro Wiper (manufactured by Daio Paper Corporation) are stacked and folded on 16 pages of cloth, and each Example (including each reference example; the same applies hereinafter) and each Comparative Example are sterilized and virus-free. 2 mL of the activating composition was added dropwise. Using this, the glass of 50 × 20 mm was wiped back and forth twice, and the time until it was dried was measured.
The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
◯: Dry within 30 seconds Δ: Dry in 30 to 45 seconds ×: Dry in 45 seconds or more If the evaluation is Δ or more, the volatility is good.

<Effect test on resin>
It was evaluated by the 1/4 ellipsoid method (described in Molding Processing Vol. 26 (2014) No. 10, p. 482, published by the Japan Plastic Molding Processing Society). A test piece (127 mm × 10 mm × thickness 1.6 mm (manufactured by Nippon Test Panel Co., Ltd.)) made of ABS resin or acrylic resin is placed along the circumference of an elliptical jig (major axis 127 mm, minor axis 38.1 mm). Two sheets of Pro-Wipe Soft Micro Wiper (manufactured by Daio Paper Co., Ltd.) folded in four were placed on top of the fixed product, and 5 mL of the sterilizing / virus-inactivating composition of each Example and Comparative Example was added dropwise to room temperature 20. The test piece was allowed to stand for 84 hours in an environment of ° C. and humidity of 50%. The presence or absence of cracks in the test piece was visually observed, and the distance from the end of the test piece on the short axis side of the 1/4 elliptical jig to the crack generation position was measured. The critical strain was calculated.
◯: No cracks occurred Δ: Cracks occurred and the critical strain was 0.6% or more ×: Cracks occurred and the critical strain was less than 0.6% If the evaluation was Δ or more, the effect on the resin was sufficient. Small and good. Further, even if the evaluation is x, if the results of the sterilization test and the virus inactivation test described later are good, the sterilization / virus inactivation composition is good.

<Sterilization test>
(1) Escherichia coli NBRC3972, Staphylococcus aureus NBRC12732, and Pseudomonas aeruginosa NBRC13275 were inoculated into a normal bouillon medium for 24 hours and 35 ° C.

(2) The bactericidal / virus-inactivating composition of each Example and each Comparative Example and the bacterial solution were mixed at a ratio (volume) of 99: 1, and after 30 seconds at room temperature, one loop loop was transplanted to SCDLP medium. Then, after culturing at 35 ° C. for 48 hours, the survival of the bacterium was determined.

(3) When turbidity was observed in the SCDLP medium, it was judged that the bacteria did not die, and when no turbidity was observed, it was judged that the bacteria had died.

Further, using the sterilizing / virus-inactivating compositions of each Example and each Comparative Example diluted 10-fold, the same operations as in (1) to (3) above were repeated, and the survival or death of the bacteria was determined.

The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
[About E. coli]
◯: Escherichia coli has died.
X: Escherichia coli was not killed.
[About Staphylococcus aureus]
◯: Staphylococcus aureus has died.
X: Staphylococcus aureus was not killed.
[About Pseudomonas aeruginosa]
◯: Pseudomonas aeruginosa has died.
X: Pseudomonas aeruginosa did not die.

<Virus inactivation test>
(Feline calicivirus inactivating effect)
(1) Feline calicivirus was infected with CRFK cells (ATCC CCL-94), which are cells derived from feline kidney, and the cells were cultured.
(2) Next, it was confirmed by the cytopathic effect (CPE) whether or not the feline calicivirus was infected.
After confirming the cytopathic effect, the cultured cells were disrupted by repeating freezing and thawing of the cultured cells.
(3) The cultured cell crushed solution was centrifuged, and the supernatant was collected to prepare a virus solution.
(4) The sterilizing / virus inactivating composition according to each Example and each Comparative Example and a virus solution are mixed at a ratio (volume) of 9: 1, and after 30 seconds at room temperature, in OPTI-MEM medium. By diluting 100 times, the action of each bactericidal / virus inactivating composition on the virus was stopped.
The solution obtained by this step is used as a sterilizing / virus-inactivating composition 30-second action virus solution.
(5) Immediately after mixing the OPTI-MEM medium and the virus solution at a ratio (volume) of 9: 1, the obtained solution was diluted 100-fold with the OPTI-MEM medium to sterilize and inactivate the virus. The action virus solution was used for 0 seconds.
(6) The sterilizing / virus inactivating composition 0-second action virus solution and the sterilizing / virus-inactivating composition 30-second action virus solution were each diluted 10-fold with OPTI-MEM medium. The medium of the 96-well microplate in which the CRFK cells were cultured was discarded, and 100 μL of the serial diluent was added.
(7) Sterilization / virus inactivating composition 0 second action Virus solution and sterilization / virus inactivation composition 30 seconds action CRFK cells to which a serially diluted solution of the virus solution was added were subjected to the conditions _{of 37 ° C. and 5% CO 2.} Then, it was cultured for 4 days.
(8) Using the CPE of cultured CRFK cells as an index, the virus infectious titer (log) of each virus solution was quantified by TCID50 (Tissue Culture Infectious Dose 50%).
(9) The above steps (1) to (8) are performed three times independently, and the average value of the virus infectious titer calculated using the sterilizing / virus inactivating composition 0-second action virus solution is used as the action time. The virus infectivity titer was taken at 0 seconds, and the average value of the virus infectivity titer calculated using the bactericidal / virus inactivating composition 30-second action virus solution was taken as the value of the virus infectivity titer at the action time of 30 seconds. ..
The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
◯: Decrease in infectivity titer of 3.0 or more Δ: Decrease in infectivity titer of 2.0 or more and less than 3.0 ×: Decrease in infectivity titer of less than 2.0 Note that the decrease in infectivity titer is 2 When it is 0.0 or more (evaluation is Δ or more), the feline calicivirus inactivating effect is good.

(Murine norovirus inactivating effect)
(1) Mouse norovirus was infected with RAW 264.7 cells (ATCC TIB-71), which is a mouse macrophage-derived cell line, and the cells were cultured.
(2) Next, it was confirmed by the cytopathic effect (CPE) whether or not the mouse norovirus was infected.
After confirming the cytopathic effect, the cultured cells were disrupted by repeating freezing and thawing of the cultured cells.
(3) The cultured cell crushed solution was centrifuged, and the supernatant was collected to prepare a virus solution.
(4) The sterilizing / virus inactivating composition according to each Example and each Comparative Example and a virus solution are mixed at a ratio (volume) of 9: 1, and after 30 seconds have passed at room temperature, 10% bovine fetal serum. By diluting 100-fold with DMEM medium containing the medium, the action of each bactericidal / virus-inactivating composition on the virus was stopped.
The solution obtained by this step was used as a bactericidal / virus-inactivating composition 30-second action virus solution.
(5) Immediately after mixing 10% fetal bovine serum-containing DMEM medium and virus solution at a ratio (volume) of 9: 1, the solution obtained by diluting 100-fold with 10% fetal bovine serum-containing DMEM medium. Was prepared as a 0-second action virus solution of the sterilizing / virus inactivating composition.
(6) Sterilizing / virus inactivating composition 0-second action virus solution and sterilizing / virus-inactivating composition 30-second action virus solution were diluted 10-fold with DMEM medium containing 10% fetal bovine serum, respectively. 50 μL of each serial diluent was added to a 96-well microplate in which 50 μL of RAW 264.7 cells were dispensed into 1 well.
(7) Sterilizing / virus inactivating composition 0 second action Virus solution and sterilizing / virus inactivating composition 30 seconds action RAW 264.7 cells to which a serially diluted solution of the virus solution was added were added at 37 ° C. and 5% CO. _It was cultured under the condition of 2 for 4 days.
(8) The virus infectious titer (countermeasure) of each virus solution was quantified by _{TCID 50} (Tissue Culture Infectious Dose 50%) using the CPE of cultured RAW 264.7 cells as an index.
(9) The above steps (1) to (8) are performed three times independently, and the average value of the virus infectious titer calculated using the sterilizing / virus inactivating composition 0-second action virus solution is used as the action time. The virus infectivity titer was taken at 0 seconds, and the average value of the virus infectivity titer calculated using the bactericidal / virus inactivating composition 30-second action virus solution was taken as the value of the virus infectivity titer at the action time of 30 seconds. ..
The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
◯: Decrease in infectivity titer of 3.0 or more Δ: Decrease in infectivity titer of 2.0 or more and less than 3.0 ×: Decrease in infectivity titer of less than 2.0 Note that the decrease in infectivity titer is 2 When it is 0.0 or more (evaluation is Δ or more), the mouse norovirus inactivating effect is good.

(Influenza virus inactivating effect)
(1) Influenza virus was infected with MDCK cells, which are cell lines derived from canine renal tubular epithelial cells, and the cells were cultured.
(2) Next, it was confirmed by the cytopathic effect (CPE) whether or not the influenza virus was infected.
After confirming the cytopathic effect, the cultured cells were disrupted by repeating freezing and thawing of the cultured cells.
(3) The crushed cell culture solution was centrifuged, and the supernatant was collected to prepare a virus solution.
(4) The sterilizing / virus inactivating composition according to each Example and each Comparative Example and a virus solution were mixed at a ratio (volume) of 9: 1, and after 30 seconds at room temperature, 2 μg / mL trypsin (2 μg / mL trypsin). By diluting 100-fold with EMEM medium containing (crystals derived from bovine spleen) (hereinafter, EMEM medium containing trypsin), the action of each bactericidal / virus inactivating composition on the virus was stopped.
The solution obtained by this step was used as a bactericidal / virus-inactivating composition 30-second action virus solution.
(5) Immediately after mixing the trypsin-containing EMEM medium and the virus solution at a ratio (volume) of 9: 1, the obtained solution was diluted 100-fold with the trypsin-containing EMEM medium to sterilize and inactivate the virus. The action virus solution was used for 0 seconds.
(6) The sterilizing / virus inactivating composition 0-second action virus solution and the sterilizing / virus-inactivating composition 30-second action virus solution were each diluted 10-fold with a trypsin-containing EMEM medium. The medium of the 96-well microplate in which the MDCK cells were cultured was discarded, and 100 μL of the serial diluent was added.
(7) Sterilizing / virus inactivating composition 0 second action Virus solution and sterilizing / virus inactivating composition 30 seconds action MDCK cells to which a serially diluted solution of the virus solution was added were subjected to the conditions _{of 37 ° C. and 5% CO 2.} Then, it was cultured for 4 days.
(8) Using the CPE of cultured MDCK cells as an index, the virus infectious titer (logarithmic value) of each virus solution was quantified by _{TCID 50 (Tissue Culture Infectious Dose 50%).}
(9) The above steps (1) to (8) are performed three times independently, and the average value of the virus infectious titer calculated using the sterilizing / virus inactivating composition 0-second action virus solution is used as the action time. The virus infectivity titer was taken at 0 seconds, and the average value of the virus infectivity titer calculated using the bactericidal / virus inactivating composition 30-second action virus solution was taken as the value of the virus infectivity titer at the action time of 30 seconds. ..
The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
◯: Decrease in infectivity titer of 3.0 or more Δ: Decrease in infectivity titer of 2.0 or more and less than 3.0 ×: Decrease in infectivity titer of less than 2.0 Note that the decrease in infectivity titer is 2 If it is 0.0 or more (evaluation is Δ or more), the influenza virus inactivating effect is good.

(Human coronavirus inactivating effect)
(1) Human coronavirus was infected with MRC-5 cells, which are normal human diploid fibroblasts, and the cells were cultured.
(2) Next, it was confirmed by the cytopathic effect (CPE) whether or not the human coronavirus was infected.
After confirming the cytopathic effect, the cultured cells were disrupted by repeating freezing and thawing of the cultured cells.
(3) The crushed cell culture solution was centrifuged, and the supernatant was collected to prepare a virus solution.
(4) The sterilizing / virus inactivating composition according to each Example and each Comparative Example and a virus solution were mixed at a ratio (volume) of 9: 1, and after 30 seconds at room temperature, 5% FBS (bovine) was used. The action of each bactericidal / virus-inactivating composition on the virus was stopped by diluting 100-fold with a MEM medium containing (fetal bovine serum) (hereinafter, MEM medium containing FBS).
The solution obtained by this step was used as a bactericidal / virus-inactivating composition 30-second action virus solution.
(5) Immediately after mixing the FBS-containing MEM medium and the virus solution at a ratio (volume) of 9: 1, the obtained solution was diluted 100-fold with the FBS-containing MEM medium to sterilize and inactivate the virus. The action virus solution was used for 0 seconds.
(6) The sterilizing / virus inactivating composition 0-second action virus solution and the sterilizing / virus-inactivating composition 30-second action virus solution were each diluted 10-fold with FBS-containing MEM medium. The medium of the 96-well microplate in which MRC-5 cells were cultured was discarded, and 100 μL of the serial diluent was added.
(7) Sterilizing / virus inactivating composition 0 second action Virus solution and sterilizing / virus inactivating composition 30 seconds action MRC-5 cells to which a serially diluted solution of the virus solution was added were added at 35 ° C. and 5% CO ₂ Was cultured for 5 days under the conditions of.
(8) The virus infectious titer (countermeasure) of each virus solution was quantified by _{TCID 50} (Tissue Culture Infectious Dose 50%) using the CPE of cultured MRC-5 cells as an index.
(9) The above steps (1) to (8) are performed three times independently, and the average value of the virus infectious titer calculated using the sterilizing / virus inactivating composition 0-second action virus solution is used as the action time. The virus infectivity titer was taken at 0 seconds, and the average value of the virus infectivity titer calculated using the bactericidal / virus inactivating composition 30-second action virus solution was taken as the value of the virus infectivity titer at the action time of 30 seconds. ..
The evaluation criteria are as follows. The results are shown in Tables 1 to 3.
◯: Decrease in infectivity titer of 3.0 or more Δ: Decrease in infectivity titer of 2.0 or more and less than 3.0 ×: Decrease in infectivity titer of less than 2.0 Note that the decrease in infectivity titer is 2 When it is 0.0 or more (evaluation is Δ or more), the human coronavirus inactivating effect is good.

From Tables 1 to 3, it was found that the bactericidal / virus inactivating composition according to the examples exhibited an excellent bactericidal effect and an excellent virus inactivating effect.
It was found that even if the bactericidal / virus-inactivating composition was diluted with water, it exerted a sufficient bactericidal effect.
Further, the bactericidal / virus inactivating composition according to the examples exhibits the above-mentioned excellent bactericidal effect and excellent virus inactivating effect even without blending a lower alcohol (alcohol). Sterilization / virus inactivity according to Reference Example 1, Examples 2 to 7, Reference Example 2, Examples 9, 10, Reference Examples 3, 4, Examples 13 to 17, Reference Example 5, Examples 19, 24, 25. Since the chemical composition does not contain a lower alcohol (alcohol) or has a very low concentration of the lower alcohol, it can be used for an object to be washed made of a material such as ABS resin or acrylic resin. The effect of preventing the appearance of the object to be washed from being spoiled without damaging the object to be washed is remarkable. Further, since the sterilizing / virus-inactivating compositions according to Examples 20 and 21 contain a lower alcohol but have a low concentration, even if they are used for an object to be washed made of a material such as ABS resin or acrylic resin, they may be used. It is possible to sufficiently prevent the appearance from being spoiled. The sterilizing / virus-inactivating composition according to Examples 22 and 23 contains a certain amount of lower alcohol, and when used in an object to be washed made of a material such as ABS resin or acrylic resin, the object to be washed is damaged. However, as with the bactericidal / virus inactivating composition according to the other examples, it is possible to exert an excellent bactericidal effect and an excellent virus inactivating effect, and to prevent infections caused by bacteria and viruses. It can be sufficiently prevented.
The sanitary material containing the bactericidal / virus inactivating composition according to such an example can exhibit an excellent bactericidal effect and an excellent virus inactivating effect, and can sufficiently prevent infectious diseases caused by bacteria and viruses. It can be prevented.

Claims (7)

Amine compounds with an acid dissociation constant (pKa) of 9.0 or more and a vapor pressure of 90 Pa or more.
Contains cationic surfactants
The cationic surfactant is a quaternary ammonium compound and is
The concentration of the amine compound is 0.3% by mass or more,
A bactericidal / virus-inactivating composition characterized by having a pH of 9.0 to 13.0. The quaternary ammonium compound is selected from the group consisting of tetraalkylammonium salt, didecylmethylpolyoxyethylammonium salt, alkylbenzyldimethylammonium salt, cetylpyridinium salt, benzethonium salt, and alkyldimethylhydroxyethylammonium salt. The bactericidal / virus-inactivating composition according to claim 1, which comprises at least one type. The sterilization / virus inactivity according to claim 1 or 2, wherein the total concentration of the anionic surfactant, the nonionic surfactant, and the amphoteric ion surfactant in the sterilization / virus inactivation composition is 2% by mass or less. Chemical composition. The sterilizing / virus inactivating composition according to any one of claims 1 to 3, wherein the concentration of the lower alcohol in the sterilizing / virus inactivating composition is 20% by mass or less. The bactericidal / virus-inactivating composition according to any one of claims 1 to 4, which is a norovirus inactivating composition. The bactericidal / virus-inactivating composition according to any one of claims 1 to 5, which is used on the surface of a hard material. A hygienic material comprising the bactericidal / virus-inactivating composition according to any one of claims 1 to 6.