JP6856495B2 - Power supply device for electrophoresis device, electrophoresis device, and power supply control method for electrophoresis device - Google Patents

Description

The present disclosure relates to a power supply device for an electrophoresis device, an electrophoresis device, and a power supply control method for the electrophoresis device.

Conventionally, a measuring device has been known in which a voltage supplied from a power supply device is applied to a measuring tool to allow a current of a predetermined magnitude to flow through the measuring tool to perform measurement. As a power source used for such a measuring device, for example, Patent Document 1 describes a power source device for an electrophoresis device.

Japanese Unexamined Patent Publication No. 2002-174623

In the above measuring device, a current of a predetermined magnitude is passed through the measuring tool to perform measurement, but the accuracy of the voltage applied from the power supply device to the measuring tool is low, and a current of a predetermined magnitude flows through the measuring tool. Sometimes it wasn't. For example, in the power supply device described in Patent Document 1, a current having a magnitude of a current value may not flow through the measuring tool due to fluctuations in the measuring tool or the like.

On the other hand, when trying to improve the accuracy of the voltage applied from the power supply device, the required current tends to be large, and the power supply device itself tends to be large.

The present disclosure provides a power supply device for an electrophoresis device, a power supply control method for an electrophoresis device, and a power supply control method for an electrophoresis device, which can make the magnitude of applied voltage highly accurate with a small current. With the goal.

The power supply device for the electrophoresis device according to the first aspect of the present disclosure is a power supply device for an electrophoresis device that uses a measuring tool having a capillary, and generates a voltage generating unit and a voltage generated by the generating unit. A Cockcroft Walton circuit that amplifies and outputs, an electrode pair consisting of first and second terminals separated by a predetermined interval for applying a voltage output from the Cockcroft Walton circuit to the capillary, and the electrode. A signal corresponding to the magnitude of the actual applied voltage applied to the measuring instrument and a signal corresponding to the magnitude of the assumed voltage applied to the measuring instrument are input by the pair, and the actual applied voltage is Based on the comparison result of the comparison circuit that compares the magnitude with the magnitude of the assumed voltage and the comparison result of the comparison circuit, the generator is generated so that the magnitude of the actual applied voltage and the magnitude of the assumed voltage match. It is provided with a correction unit for correcting the magnitude of the voltage to be applied.

The power supply device for the electrophoresis device of the second aspect of the present disclosure is a derivation unit for deriving the magnitude of the actual applied voltage applied to the measuring tool in the power supply device for the electrophoresis device of the first aspect. , Are further prepared.

The power supply device for the electrophoresis device of the third aspect of the present disclosure is the power supply device for the electrophoresis device of the second aspect, and the lead-out unit outputs the measuring tool to the measuring tool from the Cockcroft-Walton circuit. By applying the applied voltage, the magnitude of the actual applied voltage is derived based on the magnitude of the current flowing through the measuring instrument.

The power supply device for the electrophoresis device according to the fourth aspect of the present disclosure is the power supply device for the electrophoresis device according to any one of the first to third aspects, wherein the generation unit boosts the input voltage. This is an inverter transformer circuit that outputs the voltage, and the correction unit corrects the input voltage input to the generation unit.

The power supply device for the electrophoresis device according to the fifth aspect of the present disclosure is the power supply device for the electrophoresis device according to any one of the first to fourth aspects, in which the output of the Cockcroft-Walton circuit and the output of the Cockcroft Walton circuit are used. Further, a first resistance element provided between the first terminal and a second resistance element provided between the second terminal and the ground and having a resistance value lower than that of the first resistance element. The input of the comparison circuit is connected to the node between the second terminal and the second resistance element.

The power supply device for the electrophoresis device of the sixth aspect of the present disclosure is the power supply device for the electrophoresis device of the fifth aspect, in which the Cockcroft-Walton circuit and the first terminal are located between the first terminal. A low-pass filter having a resistance element was further provided.

The power supply device for the electrophoresis device according to the seventh aspect of the present disclosure is the power supply device for the electrophoresis device according to any one of the first to sixth aspects, and the measuring tool is used to run a sample. It is a disposable type chip equipped with the capillary.

The power supply device for the electrophoresis device according to the eighth aspect of the present disclosure is the power supply device for the electrophoresis device according to any one of the first to seventh aspects, and the mark applied to the measuring tool. A measuring unit for measuring the magnitude of the applied voltage was further provided.

The electrophoresis apparatus according to the ninth aspect of the present disclosure includes a power supply device for the electrophoresis apparatus according to any one of the first to eighth aspects, a storage unit for storing the magnitude of the assumed voltage, and a storage unit for storing the magnitude of the assumed voltage. The storage unit includes a control unit that controls the comparison circuit to input a signal corresponding to the magnitude of the assumed voltage stored in the storage unit.

The electrophoresis apparatus according to the tenth aspect of the present disclosure is applied to the measuring instrument based on the measurement results of the power supply device for the electrophoresis apparatus according to the eighth aspect and the measurement unit of the power supply device for the electrophoresis apparatus. A detection unit for detecting an abnormality in the actual applied voltage is provided.

The power supply control method for an electrophoresis apparatus according to the eleventh aspect of the present disclosure is a power supply control method for an electrophoresis apparatus using a measuring instrument having a capillary, in which a generator causes a current of a predetermined magnitude to flow through the measuring instrument. The generated voltage is amplified by the Cockcroft Walton circuit and output, and the actual applied voltage output from the Cockcroft Walton circuit is applied to the capillary at predetermined intervals. A signal corresponding to the magnitude of the actual applied voltage applied to the measuring tool by the electrode pair consisting of the first terminal and the second terminal, and a signal corresponding to the magnitude of the assumed voltage applied to the measuring tool. A comparison circuit to which a signal is input compares the magnitude of the actual applied voltage with the magnitude of the assumed voltage, and based on the comparison result, the magnitude of the actual applied voltage matches the magnitude of the assumed voltage. As described above, the process of correcting the magnitude of the voltage generated by the generation unit is included.

According to the present disclosure, there is an effect that the accuracy of the magnitude of the applied voltage can be made high with a small current.

It is a block diagram which shows the structure of an example of the electrophoresis apparatus of embodiment. It is a block diagram which shows the outline of an example of the structure of the power supply device of an embodiment. It is a circuit diagram which shows an example of the structure of the power supply device of an embodiment. It is a flowchart which shows an example of the operation flow of the power-source device of embodiment.

Hereinafter, examples of embodiments for carrying out the technique of the present disclosure will be described in detail with reference to the drawings.

FIG. 1 shows a configuration diagram showing an outline of an example of the electrophoresis apparatus 1 of the present embodiment. As shown in FIG. 1, the electrophoresis apparatus 1 of the present embodiment includes a control unit 10, a power supply device 20, an introduction side electrode 22, an discharge side electrode 24, and an analysis unit 26. The electrophoresis apparatus 1 of the present embodiment is an apparatus that performs measurement (analysis) using a capillary electrophoresis method on a sample 40 that migrates a capillary 4 of a microchip 2. The microchip 2 of the present embodiment is an example of the measuring tool of the present disclosure, and more specifically, the sample 40, the running solution 44, or the diluent (including the running solution 44) filled in the capillary 4 of the microchip 2 is included. The microchip 2 containing the solution of the sample 42 or the like diluted by), that is, the microchip 2 in which a sufficient current flows is an example of the measuring tool of the present disclosure. However, the technique of the present disclosure may be carried out with the microchip 2 in a state where a solution is not contained as long as a sufficient current flows.

The microchip 2 is a disposable type chip intended to be discarded after one or a specific number of analyzes, and is formed of, for example, silica or the like. As shown in FIG. 1, the microchip 2 of the present embodiment has a capillary 4, an introduction tank 6, and a discharge tank 8 which are separation flow paths. The introduction tank 6 is a tank into which the running solution 44, which functions as a so-called buffer in capillary electrophoresis, and the sample 40 to be analyzed are introduced. Examples of the running solution 44 include 100 mM malic acid-arginine buffer (pH 5.0) + 1.5% sodium chondroitin sulfate. Sample 40 is, for example, blood or the like.

Capillary 4 is a place where analysis using capillary electrophoresis is performed. To give an example of the dimensions of the capillary 4, the shape of the cross section is preferably a circle having a diameter of 25 μm to 100 μm, or a rectangle having a side length of 25 μm to 100 μm, and the length is preferably about 30 mm. It is not particularly limited.

The discharge tank 8 is located on the downstream side of the flow in the capillary electrophoresis method with respect to the capillary 4. For example, a discharge nozzle (not shown) is attached to the discharge tank 8. This discharge nozzle is for discharging the sample 40 and the running solution 44 whose analysis has been completed by a suction pump (not shown).

The power supply device 20, which will be described in detail later, is for generating a voltage required for capillary electrophoresis, and for example, generates a voltage of about 1.5 kV. As shown in FIG. 1, the power supply device 20 of the present embodiment includes a first output terminal 70 and a first input terminal 80. The first output terminal 70 of the present embodiment is an example of the first terminal of the present disclosure, and the first input terminal 80 is an example of the second terminal of the present disclosure. Further, the terminal pair of the first output terminal 70 and the first input terminal 80 is an example of the electrode pair of the present disclosure.

The introduction side electrode 22 is connected to the first output terminal 70, and the discharge side electrode 24 is connected to the first input terminal 80. That is, the introduction side electrode 22 of the present embodiment is also an example of the first terminal of the present disclosure together with the first output terminal 70, and the discharge side electrode 24 of the present embodiment is also the second terminal of the present disclosure together with the first input terminal 80. As an example, the electrode pair of the introduction side electrode 22 and the discharge side electrode 24 is an example of the electrode pair of the present disclosure. The introduction side electrode 22 and the discharge side electrode 24 are rod-shaped electrodes made of Cu having a cross-sectional diameter of 0.8 mm to 1.0 mm, for example. The introduction side electrode 22 is immersed in the introduction tank 6, the discharge side electrode 24 is immersed in the discharge tank 8, and a voltage is applied to the capillary 4. If a voltage is applied to the capillary 4 and the sample 40 filled in the capillary 4 of the microchip 2 can be electrophoresed on the introduction side electrode 22 and the discharge side electrode 24, any part of the microchip 2 can be run. May be connected with. A preferable connection position is a position sandwiching the capillary 2 like the introduction tank 6 and the discharge tank 8.

The analysis unit 26 of the present embodiment executes, for example, measurement of absorbance, and includes a light emitting unit 28, a light source unit 30, a light receiving unit 32, and a detection unit 34, as shown in FIG. The light source unit 30 is for generating light used for absorbance measurement, and includes, for example, a laser element (not shown). For example, when analyzing the concentration of hemoglobin species such as hemoglobin A1c in blood, the light source unit 30 generates light having a wavelength of 415 nm, but the light source unit 30 is not particularly limited and emits light having a wavelength corresponding to the analysis target. Anything that occurs will do. The light emitting unit 28 is connected to the light source unit 30 via, for example, an optical fiber, and irradiates the light from the light source unit 30 toward a part of the capillary 4 as irradiation light. The light receiving unit 32 is a portion that receives light from the capillary 4, and is connected to the detection unit 34 via, for example, an optical fiber. The detection unit 34 detects the light received by the light receiving unit 32.

The control unit 10 is for controlling the operation of each part of the electrophoresis apparatus 1, and performs a series of controls for realizing the analysis by the electrophoresis apparatus 1. The control unit 10 includes a CPU (Central Processing Unit), a storage unit 11 (see FIG. 2) such as a ROM (Read Only Memory) and a RAM (Random Access Memory), an input / output interface, and the like. Examples of such a control unit 10 include a so-called microprocessor and the like.

When the measurement (analysis) is performed by the electrophoresis apparatus 1, the electrophoresis liquid 44 is first introduced from the introduction nozzle 46 into the introduction tank 6. The running solution 44 fills the introduction tank 6, the capillary 4, and the discharge tank 8. Then, a predetermined amount of the sample 40 such as blood stored in the sample container 42 is introduced into the introduction nozzle 46. When it is necessary to dilute the sample 40 for analysis, the sample 40 diluted by the dilution means (not shown) is used. When a voltage is applied from the power supply device 20 to the introduction side electrode 22 and the discharge side electrode 24, the separation of the sample 40 starts. As the voltage applied to the sample 40 elapses, a specific component such as hemoglobin A1c is separated from the other components. This separated specific component moves the capillary 4 toward the discharge tank 8. When the specific component passes through the portion of the capillary 4 sandwiched between the light emitting unit 28 and the light receiving unit 32 during movement, a part of the irradiation light is absorbed by the specific component. Then, the unabsorbed light is measured as transmitted light by the detection unit 34, and the concentration (transmitted amount) is further detected by the principle of absorbance measurement based on the irradiation light and the transmitted light.

Next, the details of the power supply device 20 of this embodiment will be described. FIG. 2 shows a block diagram showing an outline of an example of the configuration of the power supply device 20 of the present embodiment. Further, FIG. 3 shows a circuit diagram showing an example of the configuration of the power supply device 20 of the present embodiment.

As shown in FIGS. 2 and 3, the power supply device 20 of the present embodiment includes an input control circuit 50, an enable circuit 52, an inverter transformer circuit 54, a CCW (Cockcroft-Walton circuit) circuit 56, an output short circuit protection circuit 58, and an overvoltage. It includes a protection circuit 60, a current detection circuit 62, and a voltage detection circuit 64. Further, the power supply device 20 of the present embodiment includes a first output terminal 70 and a first input terminal 80, and as described above, the microchip 2 is provided via the first output terminal 70 and the first input terminal 80. Is connected with. Further, the power supply device 20 of the present embodiment includes a second output terminal 72, a third output terminal 74, a second input terminal 82, a third input terminal 84, and a fourth input terminal 86, and these terminals are provided. It is connected to the control unit 10 via the control unit 10.

The power supply device 20 of the present embodiment has a voltage for passing a current of a predetermined magnitude required for measurement to the microchip 2 in response to an enable signal input from the control unit 10 via the second input terminal 82. It has a function of generating (hereinafter, referred to as "applied voltage"). Further, the power supply device 20 of the present embodiment corresponds to the magnitude of the assumed voltage (details will be described later) assumed to be applied to the microchip 2 in the initial process of applying the voltage for electrophoresis of the sample 40 in the capillary 4. It has a function of correcting the input voltage of the inverter transformer circuit 54 based on the magnitude of the current control signal.

The enable circuit 52 controls the input state (on / off) of the voltage to the primary side of the inverter transformer circuit 54 according to the enable signal input from the control unit 10 via the second input terminal 82. As shown in FIG. 3, the enable circuit 52 of the present embodiment includes resistance elements R1, to R3, a transistor Tr1 which is an npn type bipolar transistor, and a P channel type MOSFET (metal-oxide-semiconductor field-effect transistor). ), The transistor Tr2 is provided.

As shown in FIG. 3, the base of the grounded-emitter transistor Tr1 and the second input terminal 82 are connected via the resistance element R1, and the node between the resistance element R1 and the base of the transistor Tr1 is a transistor. It is connected to the emitter of Tr1. Further, the collector of the transistor Tr1 and the gate of the transistor Tr2 are connected, and the resistance element R3 is connected between the source of the transistor Tr2 and the gate.

The input control circuit 50 compares the magnitude of the current monitor signal (detailed later) input via the third input terminal 84 with the current control signal (detailed later) input via the fourth input terminal 86. , The magnitude of the input voltage input to the inverter transformer circuit 54 is controlled so that the magnitude of the current monitor signal and the magnitude of the current control signal match. The input control circuit 50 of the present embodiment is an example of the comparison circuit and the correction unit of the present disclosure.

As shown in FIG. 3, the input control circuit 50 is operational amplifiers Ap1, Ap2, resistance elements R4 to R13, capacitive elements C1, C2, npn-type bipolar transistors Tr3, Tr5, and N-channel MOSFETs. It has a transistor Tr4. The non-inverting input terminal of the operational amplifier Ap1 is connected to the fourth input terminal 86, and a current control signal is input via the fourth input terminal 86. The inverting input terminal of the operational amplifier Ap1 and the output are connected, and the output of the operational amplifier Ap1 is connected to the non-inverting input terminal of the operational amplifier Ap2 via the resistance element R9.

The operational amplifier Ap2 is an inverting amplifier circuit, and the inverting input terminal and the output of the operational amplifier Ap2 are connected via a resistance element R10 and a capacitance element C1 connected in parallel. Further, the inverting input terminal of the operational amplifier Ap2 is connected to the third input terminal 84 via the resistance element R8, and the current monitor signal is input via the third input terminal 84. Further, the output of the operational amplifier Ap2 and the base of the transistor Tr5 are connected via the resistance element R11. The emitter of the transistor Tr5 is grounded via a capacitor C2 and a resistance element R13 connected in series. Further, the base and the emitter of the transistor Tr5 are connected via the resistance element R12. Further, the collector of the transistor Tr5 and the drain of the transistor Tr2 of the enable circuit 52 are connected.

The base of the grounded-emitter transistor Tr3 and the second input terminal 82 are connected via a resistance element R4, and the node between the resistance element R4 and the base of the transistor Tr3 is connected to the emitter of the transistor Tr3. ing. Further, the collector of the transistor Tr3 is connected to the supply source of the drive voltage Vcc via the resistance element R6, and is connected to the gate of the transistor Tr4. A resistance element R3 is connected between the source of the transistor Tr2 and the gate. The drain of the transistor Tr4 is connected to the node between the resistance element R9 and the non-inverting input terminal of the operational amplifier Ap2, and the source is connected between the resistance element R7 and the ground. The resistance element R7 is provided between the non-inverting input terminal of the operational amplifier Ap2 and the ground.

An input control circuit 50 is connected to the input of the voltage on the primary side of the inverter transformer circuit 54, and the direct current (DC) input voltage input from the input control circuit 50 is boosted to boost the boosted AC (AC). Output voltage. The inverter transformer circuit 54 of the present embodiment is an example of the generation unit of the present disclosure.

The CCW circuit 56 amplifies the voltage output from the inverter transformer circuit 54, rectifies it to a DC voltage, and outputs the voltage. As shown in FIG. 3, the CCW circuit 56 of the present embodiment is a three-stage CCW circuit having capacitive elements C3 to C8 and diodes D1 to D6, and is six times the peak input voltage of the input voltage. A large voltage is output. This voltage becomes the actual applied voltage described later.

As shown in FIG. 3, the capacitance elements C3, C5, and C7 are connected in series, and the capacitance elements C4, C6, and C8 are also connected in series. The anode of the diode D1 is connected to the node between the capacitive element C4 and the inverter transformer circuit 54, and the cathode is connected to the node between the capacitive element C3 and the capacitive element C5. The anode of the diode D2 is connected to the node between the capacitive element C4 and the capacitive element C6, and the cathode is connected to the node between the capacitive element C3 and the capacitive element C5. The anode of the diode D3 is connected to the node between the capacitive element C4 and the capacitive element C6, and the cathode is connected to the node between the capacitive element C5 and the capacitive element C7. The anode of the diode D4 is connected to the node between the capacitive element C5 and the capacitive element C7, and the cathode is connected to the node between the capacitive element C6 and the capacitive element C8. The anode of the diode D5 is connected to the node between the capacitive element C6 and the capacitive element C8, and the cathode is connected to the capacitive element C7. The anode of the diode D6 is connected to the capacitive element C7, and the cathode is connected to the capacitive element C8.

The output short-circuit protection circuit 58 suppresses the application of a high voltage to the operational amplifier Ap4 of the current detection circuit 62 when the first output terminal 70 and the first input terminal 80 are short-circuited (short-circuited). Therefore, as shown in FIG. 3, the output short-circuit protection circuit 58 of the present embodiment includes resistance elements R20 to R23 connected in series. The resistance elements R20 to R23 of the present embodiment are examples of the first resistance element of the present disclosure. The output short-circuit protection circuit 58 of the present embodiment also functions as a low-pass filter for reducing noise that cannot be completely removed by the CCW circuit 56. Therefore, as shown in FIG. 3, the output short-circuit protection circuit 58 of the present embodiment is configured as an RC low-pass filter and includes capacitive elements C10 to C12.

The voltage output from the output short-circuit protection circuit 58 is applied to the microchip 2 via the first output terminal 70. This voltage is an example of "the actual applied voltage applied to the measuring tool by the electrode pair".

The overvoltage protection circuit 60 monitors the output voltage of the CCW circuit 56, and when the magnitude of the output voltage exceeds a predetermined magnitude (details will be described later), the current input to the primary side of the inverter transformer circuit 54 is set. By shutting off, it is possible to prevent the output voltage from becoming overvoltage.

As shown in FIG. 3, the overvoltage protection circuit 60 of the present embodiment includes operational amplifiers Ap3, resistance elements R14 to R17, capacitive elements C9, transistor Tr6 which is an npn type bipolar transistor, and transistor Tr7 which is an N-channel MOSFET. have. The output voltage of the CCW circuit 56 divided by the resistance element R18 and the resistance element R19 connected in series is input to the inverting input terminal of the operational amplifier Ap3. In the present embodiment, the resistance value of the resistance element R18 is high and the resistance value of the resistance element R19 is low (at least lower than the resistance element R18), so that the voltage of the voltage input to the non-inverting input terminal of the operational amplifier Ap3 is applied. The value is lower. As a specific example, 100 MΩ can be adopted as the resistance value of the resistance element R18, and 100 kΩ can be adopted as the resistance value of the resistance element R19.

A voltage obtained by dividing the drive voltage Vcc by the resistance elements R14 and R15 and the capacitance element C9 connected in series is input to the non-inverting input terminal of the operational amplifier Ap3. The magnitude of the voltage obtained by dividing the drive voltage Vcc is the same as the above-mentioned predetermined magnitude. The output of the operational amplifier Ap3 is connected to the collector of the grounded-emitter transistor Tr6 and the gate of the transistor Tr7 via the resistance element R16. The base of the transistor Tr6 is grounded via the resistance element R17, and is also connected to the source of the transistor Tr7. The transistor Tr6 and the resistance element R17 function as a current control circuit that controls the current input to the primary side of the inverter transformer circuit 54. Further, the drain of the transistor Tr7 is connected to the ground on the primary side of the inverter transformer circuit 54.

The current detection circuit 62 measures the current flowing through the microchip 2 by applying the applied voltage via the first output terminal 70, and obtains a current monitor signal derived based on the magnitude of the measured current. 2 Outputs via the output terminal 72. The current monitor signal output from the second output terminal 72 is input to the input control circuit 50 via the control unit 10 and the third input terminal 84. The current detection circuit 62 of the present embodiment is an example of the derivation unit of the present disclosure, and the current monitor signal is an example of a signal corresponding to the magnitude of the actual applied voltage.

As shown in FIG. 3, the current detection circuit 62 of the present embodiment includes an operational amplifier Ap4, resistance elements R24 to R26, and a capacitance element C13. The first input terminal 80 and the resistance element R24 are connected to the non-inverting input terminal of the operational amplifier Ap4. Therefore, the magnitude of the voltage input to the non-inverting input terminal of the operational amplifier Ap4 is determined by the magnitude of the current flowing through the microchip 2 and the resistance value of the resistance element R24. The resistance value of the resistance element R24 is determined, for example, according to the magnitude of the load assumed on the microchip 2.

The magnitude of the voltage input to the non-inverting input terminal of the operational amplifier Ap4 is the resistance elements R20 to R23 and the resistance when the first output terminal 70 and the second output terminal 72 are short-circuited (short-circuited) as described above. The voltage is divided by the element R24. Specifically, the magnitude _{V CCW} of the voltage output from the CCW circuit 56, a resistive element R20~R23 each resistance value _R 20 _{to R 23,} and the resistance value of the resistance element R24 and _{R 24,} the operational amplifier Ap4 size V _in the voltage input to the non-inverting input terminal of is expressed by the following equation (1).

V _in = (V _CCW / (R ₂₀ + R ₂₁ + R ₂₂ + R ₂₃ + R ₂₄ )) × R ₂₄ ... (1)

Therefore, since the magnitude of the voltage input to the non-inverting input terminal of the operational amplifier Ap4 is considerably smaller than the applied voltage, it is possible to suppress the application of a high voltage to the non-inverting input terminal of the operational amplifier Ap4. .. The resistance element R24 of the present embodiment is an example of the second resistance element of the present disclosure.

Further, the operational amplifier Ap4 is an inverting amplifier circuit, and the inverting input terminal and the output of the operational amplifier Ap4 are connected via a resistance element R26 and a capacitance element C13 connected in parallel. Further, the inverting input terminal of the operational amplifier Ap4 is grounded via the resistance element 25. Further, the output of the operational amplifier Ap4 is connected to the second output terminal 72.

The voltage detection circuit 64 measures the applied voltage output from the output short-circuit protection circuit 58, and outputs a voltage monitor signal derived based on the magnitude of the measured voltage via the third output terminal 74. The voltage monitor signal output from the third output terminal 74 is input to the control unit 10. The voltage detection circuit 64 of the present embodiment is an example of the measurement unit of the present disclosure, and the voltage monitor signal is an example of a signal corresponding to the magnitude of the actual applied voltage.

As shown in FIG. 3, the voltage detection circuit 64 of the present embodiment includes an operational amplifier Ap5, resistance elements R27 to R30, and a capacitance element C14. The non-inverting input terminal of the operational amplifier Ap5 is connected to the output short-circuit protection circuit 58 (first output terminal 70) via the resistance element R28, and is grounded via the resistance element R27. Therefore, a voltage obtained by dividing the voltage output from the CCW circuit 56 by the resistance elements R20 to R28 is input to the non-inverting input terminal of the operational amplifier Ap5. The operational amplifier Ap5 is an inverting amplifier circuit, and the inverting input terminal and the output of the operational amplifier Ap5 are connected via a resistance element R30 and a capacitance element C14 connected in parallel. Further, the inverting input terminal of the operational amplifier Ap5 is grounded via the resistance element 29. Further, the output of the operational amplifier Ap5 is connected to the third output terminal 74.

The overvoltage protection circuit 60 and the voltage detection circuit 64 both have a function of monitoring (measuring) the voltage output from the CCW circuit 56, but the voltage detection circuit 64 is routed through the output short-circuit protection circuit 58. Therefore, since the magnitude of the voltage applied to each of the overvoltage protection circuit 60 and the voltage detection circuit 64 is different, the configurations of the overvoltage protection circuit 60 and the voltage detection circuit 64 are different.

Next, the operation of this embodiment will be described.
FIG. 4 shows a flowchart showing an example of the operation flow of the power supply device 20 of the present embodiment. In the electrophoresis apparatus 1 of the present embodiment, the sample 40 is electrophoresed in the capillary 4 in the initial process of voltage application. In the electrophoresis device 1, when measuring the microchip 2 (sample 40), the control unit 10 outputs an enable signal for driving the power supply device 20. Further, the storage unit 11 of the control unit 10 stores a voltage (hereinafter, referred to as “assumed voltage”) generated when a current of a predetermined magnitude is assumed to have flowed through the microchip 2 in advance, and is stored in the assumed voltage. Outputs the corresponding current control signal.

When the enable signal is input from the control unit 10, the power supply device 20 of the present embodiment operates to generate the applied voltage shown in FIG.

First, in step S100, in the enable circuit 52, the transistor Tr1 is turned on according to the input enable signal, so that the transistor Tr2 is turned on and the drive voltage Vdd is supplied.

In the next step S102, the enable circuit 52 outputs an input voltage to the inverter transformer circuit 54. Specifically, the transistor Tr3 is turned on and the transistor Tr6 is turned off according to the enable signal. A current control signal is input to the operational amplifier Ap1 via the fourth input terminal 86. The current control signal output from the operational amplifier Ap1 and the current monitor signal are input to the operational amplifier Ap2 via the third input terminal 84, and the voltage corresponding to the magnitude of the current control signal and the magnitude of the current monitor signal is a transistor. It is output to the base of Tr5. As a result, the transistor Tr5 is turned on, and the input voltage is output to the inverter transformer circuit 54.

For convenience of explanation, the operation of step S102 is performed after step S100, but the operation of the enable circuit 52 in step S100 and the operation of the input control circuit 50 in step S102 are performed in parallel. is there. In each of the following steps as well, the operations performed in parallel with the operation of the circuit are described in an order for convenience of explanation.

In the next step S104, the inverter transformer circuit 54 outputs an AC voltage whose input voltage is boosted.

In the next step S106, the CCW circuit 56 amplifies the boosted voltage output from the inverter transformer circuit 54 (6 times in the example shown in FIG. 3) and outputs the boosted voltage. As described above, when the magnitude of the output voltage output from the CCW circuit 56 becomes equal to or larger than a predetermined magnitude according to the magnitude of the applied voltage, the output voltage is input to the primary side of the inverter transformer circuit 54. Cut off the current.

In the next step S108, the output voltage output from the CCW circuit 56 is output as the applied voltage from the first output terminal 70 via the output short-circuit protection circuit 58.

In the next step S110, the voltage detection circuit 64 generates a voltage monitor signal corresponding to the voltage output from the output short-circuit protection circuit 58 and outputs the voltage monitor signal to the control unit 10 via the third output terminal 74. The control unit 10 may detect an abnormality in the applied voltage by comparing the magnitude of the voltage monitor signal input via the third output terminal 74 with the predetermined magnitude. For example, the control unit 10 causes some abnormality when, for example, the difference in magnitude becomes equal to or greater than a predetermined threshold value according to the comparison result of comparing the magnitude of the voltage monitor signal with the predetermined magnitude. It may be considered that the voltage is high, and processing such as notifying the outside may be performed. The control unit 10 in this case is an example of the detection unit of the present disclosure.

On the other hand, an applied voltage is applied to the microchip 2 by the first output terminal 70 and the introduction side electrode 22, and the second output terminal 72 and the discharge side electrode 24, and the sample 40 is measured. At this time, a current corresponding to the actual applied voltage flows through the microchip 2 (sample 40).

Therefore, in the next step S112, the current detection circuit 62 generates a current monitor signal according to the magnitude of the voltage flowing through the microchip 2 and outputs the current monitor signal to the control unit 10 via the second output terminal 72. This current monitor signal is input to the input control circuit 50 via the control unit 10 and the first input terminal 80.

Therefore, in the next step S114, the operational amplifier Ap2 of the input control circuit 50 compares the magnitude of the input current monitor signal with the magnitude of the current control signal, and corrects the input voltage of the inverter transformer circuit 54 based on the comparison result. To do. Specifically, the transistor Tr5 of the input control circuit 50 outputs a signal having a magnitude corresponding to the difference between the magnitude of the current monitor signal and the magnitude of the current control signal to the base of the transistor Tr5. Thus, the base of the transistor Tr5 - emitter voltage V _BE of the size, the magnitude of the current monitor signal changes according to the difference between the magnitude of the current control signal in response to the change is output from the input control circuit 50 , The magnitude of the input voltage input to the inverter transformer circuit 54 changes.

After that, each operation of steps S104 to S114 is repeated again until the enable signal input from the control unit 10 instructs the operation to be stopped. When these processes are repeated, the assumed voltage stored in the storage unit 11 is updated at each step, and the current control signal corresponding to the updated assumed voltage is output in the next step. By repeating each operation of steps S104 to S114 in this way, the step is completed when a current of a predetermined magnitude corresponding to the assumed voltage flows through the microchip 2. After that, a voltage corresponding to the current of a predetermined magnitude is continuously applied to the microchip 2, and the sample 40 is electrophoresed on the capillary 4, for example, to analyze a specific component such as hemoglobin A1c in blood. Can be done. In addition, each operation of steps S104 to S114 may be repeated even during this electrophoresis.

As described above, the power supply device 20 of the above embodiment is a power supply device 10 for an electrophoresis device using a microchip 2 having a capillary 4, and is generated by an inverter transformer circuit 54 that generates a voltage and an inverter transformer circuit 54. The CCW circuit 56 that amplifies and outputs the voltage, and the terminal pair of the first output terminal 70 and the first input terminal 80 that are separated by a predetermined interval for applying the voltage output from the CCW circuit 56 to the capillary 4. An electrode pair consisting of an electrode pair, a current monitor signal corresponding to the magnitude of the actual applied voltage applied to the microchip 2 by the first output terminal 70 and the first input terminal 80, and an assumption that the voltage is applied to the microchip 2. A current control signal corresponding to the magnitude of the voltage is input, the magnitude of the actual applied voltage is compared with the magnitude of the assumed voltage, and based on the comparison result, the magnitude of the actual applied voltage matches the magnitude of the assumed voltage. An input control circuit 50 that corrects the magnitude of the voltage generated by the inverter transformer circuit 54 is provided.

That is, in the power supply device 20 of the present embodiment, when the input control circuit 50 has a magnitude of the current monitor signal based on the current actually flowing through the microchip 2 and a current of a predetermined magnitude flows through the microchip 2. , The input voltage of the inverter transformer circuit 54 is corrected based on the magnitude of the current control signal according to the magnitude of the assumed voltage applied to the microchip 2. Therefore, according to the power supply device 20 of the present embodiment, the accuracy of the magnitude of the applied voltage applied to pass a current of a predetermined magnitude to the microchip 2 can be made highly accurate.

Since the measuring tool is a disposable type microchip 2 and the load is not constant, when a conventional power supply device is used, the magnitude of the assumed voltage and the actual applied voltage based on the current actually flowing through the microchip 2 are used. The size may not match. That is, when the measuring tool is a disposable type microchip 2, a current of a predetermined magnitude may not flow through the microchip 2. Even in such a case, in the power supply device 20 of the present embodiment, as described above, a current of a predetermined magnitude can be passed regardless of the microchip 2. In other words, according to the power supply device 20 of the present embodiment, it may be possible to match the magnitude of the assumed voltage with the magnitude of the actual applied voltage based on the current actually flowing through the microchip 2.

Further, in the power supply device 20 of the present embodiment, since the boosted voltage output from the inverter transformer circuit 54 is amplified by the CCW circuit 56, a large current is not required and a high voltage applied voltage can be obtained with a relatively small current. Can be done.

Therefore, according to the power supply device 20 of the present embodiment, the accuracy of the magnitude of the applied voltage can be made high with a small current. Further, according to the power supply device 20 of the present embodiment, since the inverter transformer circuit 54 and the CCW circuit 56 are used as described above, it is possible to suppress the increase in size of the power supply device 20 and also suppress the cost thereof. Can be done.

Needless to say, the present invention is not limited to the above embodiment and can be changed in various ways. For example, the power supply device 20 of the present embodiment corrects the input voltage of the inverter transformer circuit 54 as described above in the initial process of applying the voltage for electrophoresis of the sample 40 in the capillary 4, but corrects the input voltage. The timing is not limited to this form. The timing for correcting the input voltage may be different from the timing for performing electrophoresis and may be before the timing for performing electrophoresis, but electrophoresis may occur before the correction of the input voltage is completed. Therefore, it is preferable to set the timing of the initial process as in the above embodiment.

Further, for example, the power supply device 20 of the above embodiment may be applied to a measuring device or an analyzer other than the electrophoresis device 1. As described above, the power supply device 20 is preferably applied when the load of the measuring tool varies.

Further, the current detection circuit 62 can be adjusted based on the magnitude of the assumed voltage and the magnitude of the current according to the assumed voltage, so that the magnitude of the current can be adjusted with higher accuracy.

All documents, patent applications and technical standards described herein are to the same extent as if the individual documents, patent applications and technical standards were specifically and individually stated to be incorporated by reference. Incorporated by reference in the book.

1 Electrophoresis device 10 Control unit 11 Storage unit 20 Power supply device 50 Input control circuit 54 Inverter transformer circuit 56 CCW circuit 58 Output short circuit protection circuit 62 Current detection circuit 64 Voltage detection circuit R1 to R30 Resistance element

Claims (11)

In a power supply device for an electrophoresis device using a measuring tool having a capillary,
A generator that generates voltage and
A Cockcroft-Walton circuit that amplifies and outputs the voltage generated by the generator,
An electrode pair consisting of a first terminal and a second terminal separated by a predetermined interval for applying a voltage output from the Cockcroft-Walton circuit to the capillary,
A signal corresponding to the magnitude of the actual applied voltage applied to the measuring tool by the electrode pair and a signal corresponding to the magnitude of the assumed voltage applied to the measuring tool are input, and the actual application is performed. A comparison circuit that compares the magnitude of the voltage with the magnitude of the assumed voltage,
Based on the comparison result of the comparison circuit, a correction unit that corrects the magnitude of the voltage generated by the generation unit so that the magnitude of the actual applied voltage and the magnitude of the assumed voltage match.
A power supply for an electrophoresis device equipped with. A derivation unit for deriving the magnitude of the actual applied voltage applied to the measuring tool is further provided.
The power supply device for electrophoresis according to claim 1. The lead-out unit has a magnitude of the actual applied voltage based on the magnitude of the current flowing through the measuring instrument due to the voltage output from the Cockcroft-Walton circuit being applied to the measuring instrument. To derive,
The power supply device for the electrophoresis device according to claim 2. The generator is an inverter transformer circuit that boosts the input voltage and outputs it.
The correction unit corrects the input voltage input to the generation unit.
The power supply device for an electrophoresis device according to any one of claims 1 to 3. A first resistance element provided between the output of the Cockcroft-Walton circuit and the first terminal,
A second resistance element provided between the second terminal and the ground and having a resistance value lower than that of the first resistance element is further provided.
The input of the comparison circuit is connected to the node between the second terminal and the second resistance element.
The power supply device for an electrophoresis device according to any one of claims 1 to 4. A low-pass filter having the first resistance element is further provided between the Cockcroft-Walton circuit and the first terminal.
The power supply device for the electrophoresis device according to claim 5. The measuring tool is a disposable type chip provided with the capillary on which the sample is electrophoresed.
The power supply device for an electrophoresis device according to any one of claims 1 to 6. A measuring unit for measuring the magnitude of the actual applied voltage applied to the measuring tool is further provided.
The power supply device for an electrophoresis device according to any one of claims 1 to 7. The power supply device for the electrophoresis device according to any one of claims 1 to 8.
A storage unit that stores the magnitude of the assumed voltage and
A control unit that controls the comparison circuit to input a signal corresponding to the magnitude of the assumed voltage stored in the storage unit.
An electrophoresis device equipped with. The power supply device for the electrophoresis device according to claim 8,
A detection unit that detects an abnormality in the actual applied voltage applied to the measuring tool based on the measurement results of the measurement unit of the power supply device for the electrophoresis device.
An electrophoresis device equipped with. In a power control method for an electrophoresis device using a measuring tool having a capillary,
The generator generates a voltage for passing a current of a predetermined magnitude through the measuring tool.
The generated voltage is amplified by the Cockcroft-Walton circuit and output.
Of the actual applied voltage applied to the measuring instrument by an electrode pair consisting of a first terminal and a second terminal separated by a predetermined interval for applying the actual applied voltage output from the Cockcroft-Walton circuit to the capillary. The magnitude of the actual applied voltage and the magnitude of the assumed voltage are determined by a comparison circuit in which a signal corresponding to the magnitude and a signal corresponding to the magnitude of the assumed voltage applied to the measuring instrument are input. Compare and
Based on the comparison result, the magnitude of the voltage generated by the generation unit is corrected so that the magnitude of the actual applied voltage and the magnitude of the assumed voltage match.
A power control method for an electrophoresis device that includes processing.

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