JP6840770B2 - 成長因子レベルを調節可能な活性化血小板組成物 - Google Patents
成長因子レベルを調節可能な活性化血小板組成物 Download PDFInfo
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Description
12 パルス生成回路
14 電極
16 電極
18 キュベット
20 接点
22 血小板懸濁液試料
24 試料ホルダ
26 プロセッサ
28 メモリ
30 ディスプレイ
32 ユーザ入力デバイス
34 電流検知回路
40 プロセスフロー
42 血液試料
46 PRP試料
50 活性化血小板組成物、血小板活性化組成物
54 パルスパラメータ
Claims (12)
- 成長因子を放出させるための方法であって、
パルス生成装置の電極(14、16)に対して試料(22)を位置決めするステップと、
電気パルスパラメータ(54)のセットを指定するステップであって、異なるパラメータ値が、活性化生成組成物(50)中の異なるレベルの1つまたは複数の成長因子を生じる、指定するステップと、
前記パラメータ値に従って生成される1つまたは複数の電気パルスに前記試料を曝露するステップであって、前記試料(22)は、前記1つまたは複数の電気パルスに曝露されると、前記電気パルスパラメータのセットによって少なくとも部分的に決定されるレベルの前記1つまたは複数の成長因子を有する前記活性化生成組成物(50)をもたらす、曝露するステップと
を含み、
前記試料(22)が、多血小板血漿試料、血小板懸濁液、または全血試料の1つを含み、
前記活性化生成組成物(50)中の1つまたは複数の成長因子のレベルがトロンビンを用いて活性化された組成物における成長因子のレベルと同等かそれ以上であり、
前記成長因子が血小板由来成長因子(PDGF)、上皮成長因子(EGF)、血管内皮細胞成長因子(VEGF)または形質転換成長因子ベータ1(TGFb1)のいずれかである、
方法。 - 複数の容器サイズから容器または導管サイズを選択するステップと、
前記試料(22)を、事前に選択されている前記サイズの容器内に配置するステップとを含み、前記試料を位置決めするステップは、前記試料を保持する前記容器を前記電極(14、16)間の試料ホルダ(24)内に配置するステップを含み、前記活性化生成組成物(50)は、前記容器サイズによって少なくとも部分的に決定されるレベルの前記1つまたは複数の成長因子を有する、請求項1記載の方法。 - 前記電極(14、16)の間に前記試料(22)を位置決めするステップが、前記電極(14、16)の間の導管を通して前記試料を流すステップを含み、前記活性化生成組成物(50)が、前記導管の直径または前記導管を通る前記試料(22)の流速の一方または両方によって少なくとも部分的に決定されるレベルの前記1つまたは複数の成長因子を有する、請求項1に記載の方法。
- 前記電気パルスパラメータ(54)のセットは、電圧、電界、電流、パルス幅、エネルギー密度、血小板当たりのエネルギー、またはパルス数のうちの1つまたは複数を含む、請求項1に記載の方法。
- 前記1つまたは複数の成長因子は、血小板由来成長因子、上皮成長因子、血管内皮細胞成長因子、または形質転換成長因子ベータ1のうちの1つまたは複数を含む、血小板、赤血球または白血球のうちの1つまたは複数によって放出される成長因子を含む、請求項1に記載の方法。
- 成長因子を放出させるための方法であって、
キュベットサイズを選択するステップであって、異なるキュベットサイズが、活性化生成組成物(50)中の異なるレベルの1つまたは複数の成長因子を生じる、選択するステップと、
前記選択されたサイズのキュベット(18)内に試料(22)を配置するステップと、
前記キュベット(18)をパルス生成装置の試料ホルダ(24)内に配置するステップと、
1つまたは複数の電気パルスに前記試料(22)を曝露するステップであって、前記試料(22)は、前記1つまたは複数の電気パルスに曝露されると、前記キュベットサイズによって少なくとも部分的に決定されるレベルの前記1つまたは複数の成長因子を有する前記活性化生成組成物(50)をもたらす、曝露するステップと
を含み、
前記試料(22)が、多血小板血漿試料、血小板懸濁液、または全血試料の1つを含み、
前記活性化生成組成物(50)中の1つまたは複数の成長因子のレベルがトロンビンを用いて活性化された組成物における成長因子のレベルと同等かそれ以上であり、
前記成長因子が血小板由来成長因子(PDGF)、上皮成長因子(EGF)、血管内皮細胞成長因子(VEGF)または形質転換成長因子ベータ1(TGFb1)のいずれかである、
方法。 - 電気パルスパラメータ(54)のセットを指定するステップをさらに含み、異なるパラメータ値が、前記活性化生成組成物(50)中の異なるレベルの1つまたは複数の成長因子をもたらし、前記活性化生成組成物(50)が、前記電気パルスパラメータ(54)のセットによって少なくとも部分的に決定されるレベルの前記1つまたは複数の成長因子を有する、請求項6に記載の方法。
- 前記電気パルスパラメータ(54)のセットは、電圧、電界、電流、パルス幅、エネルギー密度、またはパルス数のうちの1つまたは複数を含む、請求項7に記載の方法。
- 前記1つまたは複数の成長因子は、血小板由来成長因子、上皮成長因子、血管内皮細胞成長因子、または形質転換成長因子ベータ1のうちの1つまたは複数を含む、請求項6に記載の方法。
- 電気パルス生成システム(10)であって、
少なくとも2つの異なるサイズのキュベット(18)を受け入れるように構成された試料ホルダ(24)と、
前記試料ホルダ(24)内に存在するときに、キュベット(18)内に0.1kV/cm〜4.80kV/cmの電界強度を有する1つまたは複数の電気パルスを生成するように構成されたパルス生成回路(12)と、
ユーザ入力を受信するように構成された1つまたは複数のユーザ入力デバイス(32)と、
1つまたは複数のプロセッサ実行可能ルーチンを格納する非一時的コンピュータ可読メモリ(28)と、
前記コンピュータ可読メモリ(28)に格納された前記1つまたは複数のプロセッサ実行可能ルーチンにアクセスして実行するように構成されたプロセッサ(26)とを備え、前記プロセッサ実行可能ルーチンは、実行されると、処方される成長因子プロファイルに対応する1つまたは複数の電気パルスパラメータ(54)を指定する入力を受信するステップであって、異なる電気パルスパラメータ値が、前記成長因子プロファイルにおける異なるレベルの1つまたは複数の成長因子をもたらし、ここで前記成長因子が血小板由来成長因子(PDGF)、上皮成長因子(EGF)、血管内皮細胞成長因子(VEGF)または形質転換成長因子ベータ1(TGFb1)のいずれかである、受信するステップと、前記処方される成長因子プロファイルを有する活性化生成組成物(50)を生成するために、前記パルス生成回路(12)を使用して前記入力に基づいて1つまたは複数の電気パルスを生成するステップとを含む動作が実施されるようにする、電気パルス生成システム(10)。 - 前記入力を受信するステップは、複数の成長因子プロファイルからの前記成長因子プロファイルのユーザ選択を受信するステップを含む、請求項10に記載のシステム(10)。
- 前記1つまたは複数の電気パルスパラメータ(54)は、電圧、電界、電流、パルス幅、エネルギー密度、またはパルス数を含む、請求項10に記載のシステム(10)。
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