JP6834449B2 - 磁性粒子の分散方法及び分散装置 - Google Patents
磁性粒子の分散方法及び分散装置 Download PDFInfo
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Description
磁性粒子と溶液が入った容器の外部から磁界を作用させて、磁性粒子を溶液相と気相の界面に沿った位置に集磁し、容器を水平方向に振動させる、磁性粒子の分散方法である。磁性粒子は、前記界面の面積の90%以上となるように広がっていることが好ましい。
また、前記容器を水平方向に振動させると同時に又は振動させる前に、前記磁界を解除又は減衰させることが好ましい。
磁性粒子と溶液が入っている容器の外部から磁界を作用させて前記磁性粒子を溶液相と気相の界面に沿った位置に集磁する集磁手段と、
前記容器を水平方向に振動させる攪拌手段と、
を備えた分散装置である。
また、前記容器は一次元方向又は二次元方向に複数個配列されていても良好な分散が可能である。
図1は本発明の略円筒形容器に適用した分散装置100の正面図である。図2は本発明の略円筒形容器に適用した分散装置100の平面図である。表面磁束密度が2,243ガウス(G)のネオジム磁石10(角型:200mm×15mm×5mm、5mm方向に着磁)1個をステンレス製の固定部材11に固定し、容器ブロック52(16個の容器が搭載可能)に搭載した容器の上方で磁石を昇降可能にする昇降機13aに固定した。昇降機13aはプレート12に固定し、プレート12とともに搬送機13bに沿って攪拌ポジションまで移動可能に設けた。容器は開口径6.2mm、高さ20mmの略円筒形容器51を用いた。容器には磁性粒子70(粒径2.5μm、高分子ポリマーに磁性体を含むタイプ)を、5%牛血清アルブミンを含む希釈液にて0.08%に調製した懸濁液として50μL分注した。
図4は本発明の96穴マイクロタイタープレートに適用した分散装置200の正面図である。図5は本発明の96穴マイクロタイタープレートに適用した分散装置200の平面図である。表面磁束密度が1,840ガウス(G)のネオジム磁石20(角型:150mm×20mm×5mm、5mm方向に着磁)5個を各磁石の第2面が相互に接するように結合して(150mm×100mm×5mm)ステンレス製の固定部材21に固定し、平底96穴マイクロタイタープレート61の上方で磁石を昇降可能にする昇降機23aに固定した。昇降機23aはプレート22に固定し、プレート22とともに搬送機23bに沿って攪拌ポジションまで移動可能に設けた。各ウエルには磁性粒子80(粒径2.5μm、高分子ポリマーに磁性体を含むタイプ)を、5%牛血清アルブミンを含む希釈液にて0.08%に調製した懸濁液として100μL分注した。
実施例1と同様のネオジム磁石、容器ブロック、略円筒形容器、磁性粒子、希釈液を使用した。
実施例1と同様のネオジム磁石、容器ブロック、略円筒形容器、磁性粒子、希釈液を使用した。
11、21: 固定部材
12、22: プレート
13a、23a: 昇降機
13b、23b: 搬送機
14a、14b: 容器ブロック移送手段
15、25: シリンダ
16、26: 撹拌機
24a、24b: マイクロタイタープレート移送手段
30、40: 凍結機
51: 略円筒形容器
52: 容器ブロック
61: 平底96穴マイクロタイタープレート
70、80: 磁性粒子
100、200: 分散装置
Claims (5)
- 磁性粒子と溶液が入った容器の上方から磁界を作用させて、
前記磁性粒子を溶液相と気相の界面に沿った位置に集磁し、
前記容器を水平方向に振動させる、
磁性粒子の分散方法。 - 前記磁性粒子が前記界面の面積の90%以上となるように広がった状態で集磁することを特徴とする請求項1に記載の分散方法。
- 前記容器を水平方向に振動させると同時に又は振動させる前に、前記磁界を解除又は減衰させる、請求項1又は2に記載の磁性粒子の分散方法。
- 磁性粒子と溶液が入った容器の上方から磁界を作用させて前記磁性粒子を溶液相と気相の界面に沿った位置に集磁する集磁手段と、
前記容器を水平方向に振動させる攪拌手段と、
を備えた分散装置。 - 前記容器が一次元方向又は二次元方向に複数個配列されている、請求項4に記載の分散装置。
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Family Cites Families (6)
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JPS55147143A (en) * | 1979-03-29 | 1980-11-15 | Toyo Eng Corp | Agitation method |
JPH05288529A (ja) * | 1992-04-10 | 1993-11-02 | Nippon Telegr & Teleph Corp <Ntt> | レーザ干渉磁気測定方法とその測定溶液調整法及びその測定溶液 |
JP4964161B2 (ja) * | 2008-02-07 | 2012-06-27 | 株式会社日立ハイテクノロジーズ | 磁性粒子を用いた分析方法 |
JP5678248B2 (ja) * | 2010-12-21 | 2015-02-25 | メディカテック株式会社 | 試験管撹拌装置 |
JP6325833B2 (ja) * | 2014-02-06 | 2018-05-16 | 富士フイルム和光純薬株式会社 | 磁性粒子の撹拌方法 |
JP6350654B2 (ja) * | 2014-05-23 | 2018-07-04 | 株式会社島津製作所 | 磁性体粒子の操作方法 |
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