JP6832930B2 - Ｐｄ−ｌ１陽性腫瘍組織における免疫細胞を同定する方法 - Google Patents

Ｐｄ−ｌ１陽性腫瘍組織における免疫細胞を同定する方法 Download PDF

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JP6832930B2
JP6832930B2 JP2018526566A JP2018526566A JP6832930B2 JP 6832930 B2 JP6832930 B2 JP 6832930B2 JP 2018526566 A JP2018526566 A JP 2018526566A JP 2018526566 A JP2018526566 A JP 2018526566A JP 6832930 B2 JP6832930 B2 JP 6832930B2
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enzyme
detection
tumor
primary antibody
tissue sample
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JP2018536163A (ja
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チャンドラー モーガン バーチ，
チャンドラー モーガン バーチ，
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ヴェンタナ メディカル システムズ， インク．
ヴェンタナ メディカル システムズ， インク．
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    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2812—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD4
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    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
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    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
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    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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JP2018526566A 2015-11-22 2016-11-21 Ｐｄ−ｌ１陽性腫瘍組織における免疫細胞を同定する方法 Active JP6832930B2 (ja)

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US201562258493P 2015-11-22 2015-11-22
US62/258,493 2015-11-22
PCT/EP2016/078237 WO2017085307A1 (en) 2015-11-22 2016-11-21 Methods of identifying immune cells in pd-l1 positive tumor tissue

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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102360693B1 (ko) * 2014-07-11 2022-02-08 벤타나 메디컬 시스템즈, 인코포레이티드 항-pd-l1 항체 및 이의 진단 용도
IL319966A (en) 2017-03-16 2025-05-01 Alpine Immune Sciences Inc CD80 Variant Immune Modulator Proteins and Uses Thereof
WO2019241758A1 (en) 2018-06-15 2019-12-19 Alpine Immune Sciences, Inc. Pd-1 variant immunomodulatory proteins and uses thereof
US20220177587A1 (en) 2018-09-19 2022-06-09 Alpine Immune Sciences, Inc. Methods and uses of variant cd80 fusion proteins and related constructs
CN112740042B (zh) 2018-09-20 2025-05-23 文塔纳医疗系统公司 基于大小门控以分析流式细胞术数据
CN112789684B (zh) * 2018-10-01 2025-05-09 文塔纳医疗系统公司 用于预测对pd-1轴导向疗法的应答的方法和系统
EP3887547A1 (en) 2018-11-29 2021-10-06 Ventana Medical Systems, Inc. Personalized ctdna disease monitoring via representative dna sequencing
CN115552248A (zh) * 2020-05-07 2022-12-30 文塔纳医疗系统公司 用于评价肿瘤样品中egfr和egfr配体表达的组织化学系统和方法
CN111830256A (zh) * 2020-07-01 2020-10-27 北京基谱生物科技有限公司 一种石蜡切片免疫荧光多重染色试剂盒及使用方法
WO2022059601A1 (ja) * 2020-09-16 2022-03-24 コニカミノルタ株式会社 可視化方法および情報取得方法
CN118786346A (zh) * 2022-03-07 2024-10-15 阿斯利康(瑞典)有限公司 用于预测患者对免疫疗法的应答的方法
WO2023172883A1 (en) 2022-03-07 2023-09-14 Alpine Immune Sciences, Inc. Immunomodulatory proteins of variant cd80 polypeptides, cell therapies thereof and related methods and uses
JP2025526652A (ja) * 2022-08-08 2025-08-15 ヴェンタナ メディカル システムズ， インク． デジタル化された病理スライドの定量化のためのシステムおよび方法
WO2024215528A1 (en) 2023-04-13 2024-10-17 Ventana Medical Systems, Inc. Proliferation assay for fixed solid tumors
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000024760A1 (en) * 1998-10-26 2000-05-04 Ventana Medical Systems, Inc. DETECTION OF HUMAN PAPILLOMA VIRUS IN PAPANICOLAOU (Pap) SMEARS
US6544798B1 (en) * 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6195451B1 (en) * 1999-05-13 2001-02-27 Advanced Pathology Ststems, Inc. Transformation of digital images
US8071322B2 (en) * 2002-11-12 2011-12-06 Epitomics, Inc. Method for identifying differentially expressed proteins
WO2012003476A2 (en) * 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Hapten conjugates for target detection
AU2013240090B2 (en) * 2012-03-27 2017-01-05 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
CA2873402C (en) * 2012-05-15 2023-10-24 Bristol-Myers Squibb Company Cancer immunotherapy by disrupting pd-1/pd-l1 signaling
WO2014194293A1 (en) * 2013-05-30 2014-12-04 Amplimmune, Inc. Improved methods for the selection of patients for pd-1 or b7-h4 targeted therapies, and combination therapies thereof
WO2015013389A2 (en) * 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Anti-galectin-1 (gal1) monoclonal antibodies and fragments thereof for neutralizing gal1
WO2015088930A1 (en) * 2013-12-10 2015-06-18 Merck Sharp & Dohme Corp. Immunohistochemical proximity assay for pd-1 positive cells and pd-ligand positive cells in tumor tissue
ES2713438T3 (es) * 2014-02-24 2019-05-21 Ventana Med Syst Inc Amplificación de señal de análogos de quinona metidas

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JP2018536163A (ja) 2018-12-06
WO2017085307A1 (en) 2017-05-26
AU2016357478B2 (en) 2023-07-06
EP3377530B1 (en) 2021-06-30
ES2886600T3 (es) 2021-12-20
CA3005804C (en) 2022-03-29
EP3377530A1 (en) 2018-09-26
US20180372747A1 (en) 2018-12-27
US11899016B2 (en) 2024-02-13
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