JP6774186B2 - ゲル状局所止血剤 - Google Patents
ゲル状局所止血剤 Download PDFInfo
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- JP6774186B2 JP6774186B2 JP2016012343A JP2016012343A JP6774186B2 JP 6774186 B2 JP6774186 B2 JP 6774186B2 JP 2016012343 A JP2016012343 A JP 2016012343A JP 2016012343 A JP2016012343 A JP 2016012343A JP 6774186 B2 JP6774186 B2 JP 6774186B2
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- collagen
- polypeptide
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Description
−(Pro−X−Gly)n− (1)
(式中、XはPro又はHypを表し、nは2〜20000の整数を表す)
で表されるペプチドフラグメントを有するコラーゲン様ポリペプチドは、撹拌羽根や高速カッター羽根等の媒体を用いて機械的にゲル化基材中に分散させても、その構造や作用がほとんど損なわれないことを見出した。
H−(Pro−Y−Gly)k−OH ・・・(2)
H−(Y−Gly−Pro)k−OH ・・・(3)
H−(Gly−Pro−Y)k−OH ・・・(4)
(式中、Yはヒドロキシプロリン又はプロリン、kは1〜10又は1〜5の整数)
(1)コラーゲン様ポリペプチドの凍結乾燥品の調製
コラーゲン様ポリペプチドの0.5%水溶液(JNC株式会社製、ピュアコラ0.5%水溶液)をステンレス製各型バットに流し込み、そのバットを凍結乾燥機にセットし、−30℃で、6時間凍結乾燥後、更に30℃で、24時間二次乾燥し、コラーゲン様ポリペプチドの凍結乾燥品を得た。
ワセリン(ワセリン、日興製薬株式会社製、日本薬局方規格適合品)10kgとコラーゲン様ポリペプチドの凍結乾燥塊50gを高速カッター機(株式会社クイジナート サンエイ、SBC-1000J)に充填し、回転数13,000〜17,000rpmで5分間攪拌し、分散した液をステンレス容器に移し温度70℃下で更にパドルミキサーで15分間攪拌しゲルを得た。得られたゲルを顕微鏡で観察することにより、コラーゲン様ポリペプチドの分散性を確認した。
顕微鏡観察した結果の写真を図1に示す。顕微鏡観察の結果、ゲル中にコラーゲン様ポリペプチドが分散していること、及び、コラーゲン様ポリペプチドが分解されていないことが確認された。
実施例1と同様に、ワセリンと0.5%合成コラーゲン水溶液の量を変えて、様々な合成コラーゲン濃度(0.056〜60wt%)のコラーゲン様ポリペプチド含有ゲルを調製した。得られたコラーゲン様ポリペプチド含有ゲルについて、止血機能、分散性能、及び粘度を測定し、止血剤としての性能を評価した。
Claims (1)
- 下記一般式(1) :
−(Pro−X−Gly)n−(1)
(式中、XはPro又はHypを表し、nは5〜9000の整数を表す)
で表されるペプチドフラグメントを有するポリペプチド及びワセリンからなり、
前記ポリペプチドの含有量が0.214%〜9.091%(wt%)であることを特徴とするゲル状局所用止血剤。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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JP2015013091 | 2015-01-27 | ||
JP2015013091 | 2015-01-27 |
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JP2016137246A JP2016137246A (ja) | 2016-08-04 |
JP6774186B2 true JP6774186B2 (ja) | 2020-10-21 |
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JP2016012343A Active JP6774186B2 (ja) | 2015-01-27 | 2016-01-26 | ゲル状局所止血剤 |
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Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005058499A (ja) * | 2003-08-13 | 2005-03-10 | Masao Tanihara | 生体材料 |
ATE530576T1 (de) * | 2007-08-01 | 2011-11-15 | Ethicon Inc | Collagen-assoziierte peptide und ihre verwendung |
US8658193B2 (en) * | 2012-06-08 | 2014-02-25 | Robert J. Greenwald | Styptic storage and delivery |
JP5915427B2 (ja) * | 2012-07-11 | 2016-05-11 | Jnc株式会社 | 止血材 |
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