JP6742729B2 - 宿主細胞および使用方法 - Google Patents

宿主細胞および使用方法 Download PDF

Info

Publication number
JP6742729B2
JP6742729B2 JP2015561647A JP2015561647A JP6742729B2 JP 6742729 B2 JP6742729 B2 JP 6742729B2 JP 2015561647 A JP2015561647 A JP 2015561647A JP 2015561647 A JP2015561647 A JP 2015561647A JP 6742729 B2 JP6742729 B2 JP 6742729B2
Authority
JP
Japan
Prior art keywords
host cell
polypeptide
acid sequence
pdi1
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2015561647A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016514957A5 (https=
JP2016514957A (ja
Inventor
ヨンファン、ジン
ユアン、チュー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline LLC
Original Assignee
GlaxoSmithKline LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GlaxoSmithKline LLC filed Critical GlaxoSmithKline LLC
Publication of JP2016514957A publication Critical patent/JP2016514957A/ja
Publication of JP2016514957A5 publication Critical patent/JP2016514957A5/ja
Application granted granted Critical
Publication of JP6742729B2 publication Critical patent/JP6742729B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/04—Oxidoreductases acting on sulfur groups as donors (1.8) with a disulfide as acceptor (1.8.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21061—Kexin (3.4.21.61), i.e. proprotein convertase subtilisin/kexin type 9
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Cardiology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Hospice & Palliative Care (AREA)
  • Botany (AREA)
JP2015561647A 2013-03-06 2014-03-06 宿主細胞および使用方法 Expired - Fee Related JP6742729B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361773329P 2013-03-06 2013-03-06
US61/773,329 2013-03-06
PCT/US2014/021137 WO2014138371A1 (en) 2013-03-06 2014-03-06 Host cells and methods of use

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019044077A Division JP2019122395A (ja) 2013-03-06 2019-03-11 宿主細胞および使用方法

Publications (3)

Publication Number Publication Date
JP2016514957A JP2016514957A (ja) 2016-05-26
JP2016514957A5 JP2016514957A5 (https=) 2017-04-13
JP6742729B2 true JP6742729B2 (ja) 2020-08-19

Family

ID=51491943

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015561647A Expired - Fee Related JP6742729B2 (ja) 2013-03-06 2014-03-06 宿主細胞および使用方法
JP2019044077A Pending JP2019122395A (ja) 2013-03-06 2019-03-11 宿主細胞および使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019044077A Pending JP2019122395A (ja) 2013-03-06 2019-03-11 宿主細胞および使用方法

Country Status (11)

Country Link
US (1) US9926570B2 (https=)
EP (1) EP2964666B1 (https=)
JP (2) JP6742729B2 (https=)
KR (1) KR20150126839A (https=)
AU (1) AU2014225678B2 (https=)
BR (1) BR112015021960A2 (https=)
CA (1) CA2903739A1 (https=)
ES (1) ES2843679T3 (https=)
RU (1) RU2663587C2 (https=)
SG (1) SG11201504771RA (https=)
WO (1) WO2014138371A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015001049A1 (en) 2013-07-04 2015-01-08 Novartis Ag O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
EP3394243A1 (en) 2015-12-22 2018-10-31 Albumedix Ltd. Improved protein expression strains
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
CN105950491B (zh) * 2016-05-23 2019-08-06 江南大学 一种高效表达碱性果胶酶的菌株及其构建与应用
US11130979B2 (en) 2017-06-20 2021-09-28 Albumedix Ltd Protein expression strains
KR101984957B1 (ko) 2017-08-09 2019-06-04 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 효모의 단백질 접힘 기작(protein-folding machinery) 개량을 통한 진세노사이드 생산 증대
US11135276B2 (en) 2019-05-17 2021-10-05 Imam Abdulrahman Bin Faisal University Method of making PDIA2 and compositions containing PDIA2
EP3992221A4 (en) 2019-06-28 2023-06-21 Mitsubishi Gas Chemical Company, Inc. RESIN COMPOSITION, RESIN FILM, LAYER PRODUCT, SEMICONDUCTOR WAFER WITH RESIN COMPOSITION LAYER, SUBSTRATE WITH RESIN COMPOSITION LAYER FOR SEMICONDUCTOR MOUNTING, AND SEMICONDUCTOR DEVICE
EP4402257A4 (en) * 2021-09-17 2025-08-27 Univ North Carolina Chapel Hill MODIFIED PROTEIN DISULFIDE ISOMERASE AND ITS USES
GB202301988D0 (en) * 2023-02-13 2023-03-29 Phenotypeca Ltd Breeding process

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077204A (en) 1984-06-21 1991-12-31 Chiron Corporation Yeast endopeptidase for basic amino-acid site cleavage, preparation and use
JP2643968B2 (ja) 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
FR2645174B1 (fr) 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5545618A (en) 1990-01-24 1996-08-13 Buckley; Douglas I. GLP-1 analogs useful for diabetes treatment
GB9015825D0 (en) 1990-07-18 1990-09-05 Ciba Geigy Ag In vitro processing of fusion proteins
ATE157703T1 (de) 1991-12-16 1997-09-15 Ciba Geigy Ag Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen
US6291205B1 (en) 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
GB9411356D0 (en) 1994-06-07 1994-07-27 Delta Biotechnology Ltd Yeast strains
CA2198968C (en) 1996-03-04 2010-02-09 Toyofumi Masuda Process for production of secretory kex2 derivatives
CA2258307C (en) * 1996-07-05 2008-02-12 Novo Nordisk A/S Method of producing heterologous short chain polypeptides by yeast with impaired yap3 protease
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
GB9713412D0 (en) 1997-06-26 1997-08-27 Delta Biotechnology Ltd Improved protein expression strains
US6361964B1 (en) * 1997-08-12 2002-03-26 Massachusetts Institute Of Technology Eukaryotic disulfide bond-forming proteins and related molecules and methods
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
GB9902000D0 (en) * 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
AU2002364586A1 (en) * 2001-12-21 2003-07-30 Delta Biotechnology Limited Albumin fusion proteins
HUE027902T2 (en) 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
US20060046253A1 (en) * 2004-09-02 2006-03-02 Suntory Limited Method for analyzing genes of industrial yeasts
DE502005005689D1 (de) 2005-05-04 2008-11-27 Signalomics Gmbh Kompetitives n-Hybrid System
US8202837B2 (en) * 2005-11-04 2012-06-19 Glaxosmithkline Llc Methods for administering hypoglycemic agents
JP5354559B2 (ja) 2005-11-24 2013-11-27 独立行政法人産業技術総合研究所 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
SG175602A1 (en) 2006-07-05 2011-11-28 Catalyst Biosciences Inc Protease screening methods and proteases identified thereby
US8034607B2 (en) 2007-09-12 2011-10-11 Wisconsin Alumni Research Foundation Methods of enhanced heterologous protein secretion
ES2537806T5 (es) * 2007-10-31 2018-10-09 Daiichi Sankyo Company, Limited Método para la secreción y producción de alto nivel de proteína
WO2009082209A1 (en) 2007-12-21 2009-07-02 Fujifilm Manufacturing Europe B.V. Improvement of the secretion yield of a protein of interest by in vivo proteolytic processing of a multimeric precursor
US20110129872A1 (en) * 2009-12-01 2011-06-02 Mogam Biotechnology Research Institute Method for a production of a recombinant protein using yeast co-expression system
RU2427645C1 (ru) * 2010-06-10 2011-08-27 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") СПОСОБ МИКРОБИОЛОГИЧЕСКОГО СИНТЕЗА ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА, ШТАММ Saccharomyces cerevisiae - ПРОДУЦЕНТ ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА (ВАРИАНТЫ)
MX2015005363A (es) * 2012-11-08 2015-11-06 Novozymes Biopharma Dk As Variantes de albumina.

Also Published As

Publication number Publication date
RU2663587C2 (ru) 2018-08-07
JP2016514957A (ja) 2016-05-26
AU2014225678B2 (en) 2016-11-17
US20160017343A1 (en) 2016-01-21
BR112015021960A2 (pt) 2017-08-29
JP2019122395A (ja) 2019-07-25
ES2843679T3 (es) 2021-07-20
EP2964666A1 (en) 2016-01-13
AU2014225678A1 (en) 2015-09-17
WO2014138371A1 (en) 2014-09-12
CA2903739A1 (en) 2014-09-12
EP2964666B1 (en) 2020-11-18
SG11201504771RA (en) 2015-09-29
US9926570B2 (en) 2018-03-27
KR20150126839A (ko) 2015-11-13
RU2015135980A (ru) 2017-04-10
EP2964666A4 (en) 2016-08-10

Similar Documents

Publication Publication Date Title
JP6742729B2 (ja) 宿主細胞および使用方法
EP2401357B1 (en) Host cells and methods of use
KR101262682B1 (ko) 유전자 발현 기술
KR101298157B1 (ko) 2-미크론 패밀리 플라스미드 및 이의 용도
JP5781986B2 (ja) 遺伝子発現技術
KR102495283B1 (ko) 재조합 숙주 세포에서 탄소 공급원 조절된 단백질 생산
KR20140015137A (ko) 분비 효율이 개선된 재조합 단백질의 생산 방법
KR20200019731A (ko) 개선된 단백질 발현 스트레인
CN105377882B (zh) 宿主细胞和使用方法
Liu et al. Targeted introduction of a diphtheria toxin resistant mutation into the chromosomal EF-2 locus of Pichia pastoris and expression of immunotoxin in the EF-2 mutants
EP3947695B1 (en) Mut- methylotrophic yeast
CA3135444C (en) Mut- methylotrophic yeast
Ramagoma Development of Yarrowia lipolytica for enhanced production of heterologous proteins
KR20140148027A (ko) 세포벽 결함 효모 변이주를 이용한 재조합 단백질 분비능이 향상된 효모 균주의 스크리닝 방법
HK40054115A (en) Carbon-source regulated protein production in a recombinant host cell
de Zulueta Pascual et al. Retion of human apolipoprotein A-II by a mutant of Saccharomyces cerevisiæ

Legal Events

Date Code Title Description
RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20161114

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170306

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170306

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180223

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180723

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20181109

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190311

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20190424

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20190607

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200525

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200729

R150 Certificate of patent or registration of utility model

Ref document number: 6742729

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees