JP6737710B2 - ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ - Google Patents

ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ Download PDF

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JP6737710B2
JP6737710B2 JP2016571250A JP2016571250A JP6737710B2 JP 6737710 B2 JP6737710 B2 JP 6737710B2 JP 2016571250 A JP2016571250 A JP 2016571250A JP 2016571250 A JP2016571250 A JP 2016571250A JP 6737710 B2 JP6737710 B2 JP 6737710B2
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polymerase
amino acid
acid sequence
modified
dna polymerase
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JP2017518750A5 (OSRAM
JP2017518750A (ja
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チョン−ヤオ・チェン
エリン・ボマティ
モリー・ホァ
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イルミナ インコーポレイテッド
イルミナ インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016571250A 2014-06-27 2015-06-25 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ Active JP6737710B2 (ja)

Applications Claiming Priority (3)

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US201462018470P 2014-06-27 2014-06-27
US62/018,470 2014-06-27
PCT/US2015/037785 WO2015200693A1 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

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JP2020079698A Division JP2020127415A (ja) 2014-06-27 2020-04-28 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ

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JP2017518750A JP2017518750A (ja) 2017-07-13
JP2017518750A5 JP2017518750A5 (OSRAM) 2018-08-02
JP6737710B2 true JP6737710B2 (ja) 2020-08-12

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JP2016571250A Active JP6737710B2 (ja) 2014-06-27 2015-06-25 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ
JP2020079698A Pending JP2020127415A (ja) 2014-06-27 2020-04-28 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ
JP2021196287A Pending JP2022033882A (ja) 2014-06-27 2021-12-02 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ

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JP2021196287A Pending JP2022033882A (ja) 2014-06-27 2021-12-02 ヌクレオチドアナログの取り込みを改善するための修飾ポリメラーゼ

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US (3) US9765309B2 (OSRAM)
EP (2) EP3161154B1 (OSRAM)
JP (3) JP6737710B2 (OSRAM)
CN (1) CN106536728A (OSRAM)
AU (2) AU2015279794B2 (OSRAM)
DK (1) DK3161154T3 (OSRAM)
ES (1) ES2788949T3 (OSRAM)
PL (1) PL3161154T3 (OSRAM)
PT (1) PT3161154T (OSRAM)
WO (1) WO2015200693A1 (OSRAM)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3161154B1 (en) * 2014-06-27 2020-04-15 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
DK3201355T3 (en) 2014-09-30 2019-10-14 Illumina Inc Modified polymerases for improved incorporation of nucleotide analogues
US10662413B2 (en) * 2016-11-01 2020-05-26 Personal Genomics, Inc. Recombinant DNA polymerase for improved incorporation of nucleotide analogues
EP3613851A4 (en) * 2017-01-09 2020-07-01 MGI Tech Co., Ltd. RECOMBINANT DNA POLYMERASE
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
US20220127587A1 (en) 2017-02-13 2022-04-28 IsoPlexis Corporation Polymerase enzyme from pyrococcus furiosus
WO2018148724A1 (en) * 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018197950A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
ES2882608T3 (es) 2017-04-23 2021-12-02 Illumina Inc Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN108795900B (zh) * 2017-04-27 2021-02-02 深圳华大智造科技股份有限公司 Dna聚合酶及其制备方法
EP3862427A4 (en) * 2018-09-03 2022-04-27 BGI Shenzhen RECOMBINANT KOD POLYMERASE
US20230011240A1 (en) 2018-09-11 2023-01-12 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
KR20210084349A (ko) * 2018-10-31 2021-07-07 일루미나, 인코포레이티드 중합효소, 조성물, 및 사용 방법
SG11202012559SA (en) * 2018-12-05 2021-01-28 Illumina Inc Polymerases, compositions, and methods of use
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
AU2020352503A1 (en) 2019-09-23 2022-05-19 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US20240392263A1 (en) * 2020-10-21 2024-11-28 Bgi Shenzhen Recombinant kod polymerase
AU2021453871A1 (en) 2021-06-29 2024-01-25 Agilent Technologies, Inc. Polymerase mutants and use with 3'-oh unblocked reversible terminators
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
TWI869710B (zh) * 2021-09-29 2025-01-11 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024155578A1 (en) * 2023-01-16 2024-07-25 The Regents Of The University Of California Engineered polymerase for threose nucleic acid synthesis
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
JP4990886B2 (ja) * 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
US20070022459A1 (en) 2005-07-20 2007-01-25 Gaebel Thomas M Jr Method and apparatus for boundary-based network operation
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
US7781200B2 (en) 2006-07-14 2010-08-24 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
WO2009131919A2 (en) * 2008-04-22 2009-10-29 New England Biolabs, Inc. Polymerases for incorporating modified nucleotides
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010062779A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
CN102257136A (zh) * 2008-11-03 2011-11-23 卡帕生物系统 嵌合dna聚合酶
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
EP2707481B1 (en) * 2011-05-11 2016-07-13 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) * 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
US8819970B2 (en) 2011-08-04 2014-09-02 Diana Foster Multi-layered ornamental clothing
EP2971070B2 (en) 2013-03-14 2021-03-03 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
EP3161154B1 (en) * 2014-06-27 2020-04-15 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
DK3201355T3 (en) 2014-09-30 2019-10-14 Illumina Inc Modified polymerases for improved incorporation of nucleotide analogues

Also Published As

Publication number Publication date
PL3161154T3 (pl) 2020-10-19
EP3702471A1 (en) 2020-09-02
EP3161154A1 (en) 2017-05-03
JP2020127415A (ja) 2020-08-27
US20150376582A1 (en) 2015-12-31
EP3702471B1 (en) 2025-12-24
AU2021290289B2 (en) 2024-02-15
JP2022033882A (ja) 2022-03-02
HK1231517A1 (en) 2017-12-22
CA2951416A1 (en) 2015-12-30
US20210246435A1 (en) 2021-08-12
WO2015200693A1 (en) 2015-12-30
AU2015279794A1 (en) 2016-12-22
US12110514B2 (en) 2024-10-08
US20170355970A1 (en) 2017-12-14
PT3161154T (pt) 2020-06-08
EP3161154B1 (en) 2020-04-15
US10870836B2 (en) 2020-12-22
ES2788949T3 (es) 2020-10-23
JP2017518750A (ja) 2017-07-13
DK3161154T3 (da) 2020-06-15
CN106536728A (zh) 2017-03-22
AU2021290289A1 (en) 2022-01-27
US9765309B2 (en) 2017-09-19
AU2015279794B2 (en) 2021-09-30

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