JP6683736B2 - Packaged beverages - Google Patents

Description

  The present invention relates to a packaged beverage.

  A "dipeptide" in which two amino acids are linked has been attracting attention as a functional substance. Dipeptides can add physical properties and new functions not present in single amino acids, and are expected to have a range of applications beyond amino acids. Of these, diketopiperazine, which is a cyclic dipeptide, is known to have various physiological activities, and it is expected that demand will be expanded in the medical and pharmacological fields. Further, cyclic dipeptides have been attracting attention as a material to be incorporated into foods and drinks in the expectation of their physiologically active action, and in recent years, various foods and drinks containing cyclic dipeptides have been proposed (Patent Document 1, 2).

Japanese Patent No. 5222406 Japanese Patent No. 55766653

  However, it has been found that when a cyclic dipeptide is added to a packaged beverage, the flavor of the packaged beverage may be impaired, such as the aftertaste becomes poor or the flavor may be retained.

  An object of the present invention is to provide a packaged beverage having a good aftertaste and a good flavor while containing a cyclic dipeptide.

  As a result of intensive studies conducted by the present inventors, it was found that the flavor can be improved by making the packaged beverage have a specific pH.

  The present invention relates to a packaged beverage having a cyclic dipeptide content of 10 to 3300 ppm and a pH of 3.0 to 5.0.

  According to the present invention, it is possible to provide a packaged beverage that contains a cyclic dipeptide and has a good aftertaste and a good flavor.

  The packaged beverage according to the present invention (hereinafter also simply referred to as "beverage") contains a cyclic dipeptide. As the cyclic dipeptide, known ones can be used. Among them, from the viewpoint of imparting a uric acid level-reducing effect, cyclotryptophanyl tyrosine [Cyclo (Trp-Tyr)], cycloseryl tyrosine [Cyclo (Ser- Tyr)], cycloprolyl tyrosine [Cyclo (Pro-Tyr)], cycloglycyl tyrosine [Cyclo (Gly-Tyr)], cyclotyrosyl tyrosine [Cyclo (Tyr-Tyr)], cyclophenylalanyl tyrosine [Cyclo (Phe-Tyr)], cycloleucyl tyrosine [Cyclo (Leu-Tyr)], cyclolysyl tyrosine [Cyclo (Lys-Tyr)], cyclohistidyl tyrosine [Cyclo (His-Tyr)], cycloalanyl tyrosine [Cyclo (Ala-Tyr)], cycloglutamyl tyrosine [Cyclo (Glu-Tyr)], cyclotyrosylvaline [Cyclo (Tyr-Val)], cycloisoleucyl tyrosine [Cyclo (Ile-Tyr)], cyclothreo Nyltyrosine [Cyclo (Thr-Tyr)], Cycloaspartyl Tyrosi (Cyclo (Asp-Tyr)], cycloasparaginyl tyrosine (Cyclo (Asn-Tyr)), cycloglutaminyl tyrosine (Cyclo (Gln-Tyr)), cycloarginyl tyrosine (Cyclo (Arg-Tyr)), And an embodiment containing at least one tyrosine-containing cyclic dipeptide selected from the group consisting of cyclomethionyl tyrosine [Cyclo (Met-Tyr)]. In addition, there are various cyclic dipeptides other than tyrosine-containing cyclic dipeptides, for example, Cyclo (Ala-Ala), Cyclo (Pro-Pro), Cyclo (Gly-Pro), Cyclo (Gly-Phe), Cyclo (Gly -Leu), Cyclo (Gly-His), Cyclo (Ala-Glu), Cyclo (Arg-Val), etc.

  The cyclic dipeptide used in the present invention can be prepared according to a method known in the art. For example, it may be produced by a chemical synthesis method, an enzymatic method, a microbial fermentation method, or may be synthesized by dehydrating and cyclizing a linear peptide, and is disclosed in JP-A-2003-252896 and Journal of Peptide Science, It can also be prepared according to the method described in 10, 737-737, 2004. For example, a plant-derived peptide obtained by subjecting a raw material containing a plant-derived protein to an enzyme treatment or a heat treatment as it is, or preferably subjected to a further high-temperature heat treatment after being subjected to a purification treatment, is derived from a plant rich in a cyclic dipeptide. A heat-treated peptide product can be obtained. The means for purifying the plant-derived peptide is not particularly limited, but examples thereof include filtration, centrifugation, concentration, ultrafiltration, lyophilization, and powderization. That is, the cyclic dipeptide used in the present invention may be chemically or biologically synthesized, or may be obtained from a heat-treated product of a plant-derived peptide or a purified product thereof.

  In the present specification, the “animal and plant derived peptide” is not particularly limited, but for example, one derived from one or more raw materials selected from the group consisting of soybean, tea leaf, malt, and coffee beans can be used. That is, soybean peptide, tea peptide, malt peptide, coffee bean peptide and the like can be used. Of these, malt peptides and soybean peptides are preferred in the present invention. As the plant-derived peptide, one prepared from a plant-derived protein or a raw material containing a protein may be used, or a commercially available product may be used.

  As used herein, the term "soybean peptide" refers to a low molecular weight peptide obtained by subjecting soybean protein to enzyme treatment or heat treatment to reduce the protein into a low molecular weight protein. The soybean (scientific name: Glycine max) used as a raw material can be used without limitation on the variety and the production area, and a processed product such as a crushed product can also be used. Examples of commercially available soybean peptides include high-new AM, high-new DC, high-new HK (above, manufactured by Fuji Oil Co., Ltd.) and the like.

  The term "tea peptide" as used herein refers to a tea-derived low-molecular peptide obtained by subjecting a tea (including tea leaves and tea leaves) extract to an enzyme treatment or a heat treatment to lower the protein molecular weight. As the tea leaf used as an extraction raw material, a leaf, stem or the like of a tea leaf produced by using a tea tree (scientific name: Camellia sinensis) can be used as an extractable and drinkable portion. Also, the form is not limited to large leaves or powder. The harvest time of tea leaves can also be appropriately selected according to the desired flavor. In addition, plant extract (tea extract) containing diketopiperazine at high concentration in the above studies suppresses the production of by-products by producing without a fermentation process, and a flavorful product can be obtained. Has been revealed. Therefore, tea leaves are steamed non-fermented tea (green tea) such as sencha, bancha, hojicha, gyokuro, kabusecha, and tencha, and unfermented tea such as kettled green tea such as Ureshino tea, Aoyagi tea, and various Chinese teas. It is preferable to use.

  The term "malt peptide" as used herein refers to a malt-derived low-molecular peptide obtained by subjecting an extract obtained from malt or a crushed product thereof to an enzyme treatment or a heat treatment to reduce the protein molecular weight. The malt used as a raw material can be used without any limitation on the variety and the place of origin, but barley malt obtained by germination of barley seeds is particularly preferably used. In the present specification, barley malt may be simply referred to as malt.

  The "coffee bean peptide" referred to in the present specification is a coffee bean-derived low-molecular peptide obtained by subjecting an extract obtained from coffee beans or a ground product thereof to an enzyme treatment or a heat treatment to lower the protein molecular weight. Say. The coffee beans that are the raw material can be used without restrictions on the variety or the production area.

  As described above, by heat-treating a plant-derived peptide at a high temperature, a heat-treated plant-derived peptide rich in cyclic dipeptide can be obtained. In the present specification, the "high temperature heat treatment" is preferably a treatment at a temperature of 100 ° C. or higher and a pressure exceeding atmospheric pressure for a certain period of time. As the high-temperature and high-pressure treatment device, a pressure-resistant extraction device, a pressure cooker, an autoclave, etc. can be used according to the conditions.

  The temperature in the high temperature heat treatment is desirably 100 ° C. or higher, preferably 100 ° C. to 170 ° C., more preferably 110 ° C. to 150 ° C., and even more preferably 120 ° C. to 140 ° C. In addition, this temperature shows a value obtained by measuring the outlet temperature of the extraction column when the pressure-resistant extraction device is used as the heating device, and when the autoclave is used as the heating device, the central temperature in the pressure vessel is measured. Indicates the value.

  The pressure in the high temperature heat treatment is preferably a pressure higher than atmospheric pressure, preferably 0.101 MPa to 0.79 MPa, more preferably 0.101 MPa to 0.60 MPa, even more preferably 0.101 MPa to 0.48 MPa. Is.

  The high temperature heat treatment time is preferably 15 minutes to 600 minutes, more preferably 30 minutes to 500 minutes, still more preferably 60 minutes to 300 minutes, from the viewpoint of obtaining a treated product containing a cyclic dipeptide.

  Further, the high temperature heat treatment condition of the plant-derived peptide is not particularly limited as long as a treated product containing a cyclic dipeptide is obtained, but [temperature: pressure: time] is preferably [100 ° C to 170 ° C: 0.101 MPa to 0. 79 MPa: 15 minutes to 600 minutes], more preferably [110 ° C. to 150 ° C .: 0.101 MPa to 0.60 MPa: 30 minutes to 500 minutes], even more preferably [120 ° C. to 140 ° C .: 0.101 MPa to 0]. .48 MPa: 60 minutes to 300 minutes].

  If desired, the heat-treated plant-derived peptide product may be subjected to purification treatments such as filtration, centrifugation, concentration, ultrafiltration, freeze-drying, and pulverization. Further, if the specific cyclic dipeptide in the heat-treated plant-derived peptide is less than the desired content, other animal- or plant-derived peptides or commercially available products for the lacking specific cyclic dipeptide may be appropriately added. it can.

  The content (total content) of the cyclic dipeptide in the packaged beverage according to the present invention is 10 ppm or more, preferably 50 ppm or more, more preferably 100 ppm or more, and 500 ppm or more from the viewpoint of the physiologically active function required for the cyclic dipeptide. More preferably, from the viewpoint of flavor derived from cyclic dipeptide, it is 3300 ppm or less, preferably 1600 ppm or less, more preferably 650 ppm or less. That is, the content of the cyclic dipeptide in the packaged beverage according to the present invention is 10 to 3300 ppm, and as a suitable range, 10 to 1600 ppm, 10 to 650 ppm, 10 to 500 ppm, 10 to 100 ppm, 10 to 50 ppm, 50 to 50 ppm. 3300 ppm, 50-1600 ppm, 50-650 ppm, 50-500 ppm, 50-100 ppm, 100-3300 ppm, 100-1600 ppm, 100-650 ppm, 100-500 ppm, 500-3300 ppm, 500-1600 ppm, 500-650 ppm, 650-3300 ppm, Examples include 650 to 1600 ppm and 1600 to 3300 ppm. Of these, the content of the tyrosine-containing cyclic dipeptide (total content) is preferably 1 to 400 ppm, from the viewpoint of imparting a uric acid value reducing effect, more preferably 5 to 400 ppm, further preferably 10 to 400 ppm, 50 to 400 ppm is more preferable.

  The content of cyclic dipeptide can be measured according to a known method. For example, it can be measured using LC-MS / MS or a saccharimeter. In the present specification, the content of cyclic dipeptide refers to the value measured by LC-MS / MS. The detailed analysis conditions are as follows.

(LC-MS / MS analysis conditions)
LC equipment SHIMADZU UFLC XR
Column Agilent technologies Zorbax SB-AQ 1.8 μm 2.1 x 150 mm
Column temperature 40 ℃
Mobile phase A: 0.1% Formic acid, B: Gradient analysis flow rate of methanol Total volume 0.2 mL / min
Injection volume 2 μL
Detector AB Sciex 4000 Q TRAP ®-Turbo Spray (ESI) -Scheduled MRM (multiple reaction monitoring)
4mm above nozzle position, 7mm across
MRM detection windou 40 sec, Target Scan Time 0.5 sec
[Positive mode] Scheduled MRM analysis Ion source condition CUR20.0, CAD6, IS 5500, TEM 700, GS1 70, GS2 70

  The packaged beverage according to the present invention is not particularly limited as long as it is a packaged beverage and may be sealed or unsealed. Examples of containers for filling beverages include bottles, cans, barrels, PET bottles, paper packs, pouches, and the like. Examples of the type of beverage to be filled include beer-taste beverages, alcoholic beverages such as chu-hi, non-alcoholic beer-taste beverages, carbonated beverages, fruit juice beverages, soft drinks and nutritional drinks.

  One aspect of the packaged beverage according to the present invention is a beer-taste beverage containing alcohol. The beverage of the present aspect can be produced in the same manner as a general beer-taste beverage except that a cyclic dipeptide is used. The alcohol at this time refers to ethanol, and the content of ethanol is preferably 1% to 10% by volume, but is not particularly limited. Further, the origin of the alcohol content contained in the beer-taste beverage is not limited to fermentation and non-fermentation. Below, the manufacturing process of a general beer taste drink is shown. Common beer-taste beverages include those that use malt as a raw material and those that do not, and can be produced as follows.

  Alcohol-containing beer-taste beverages produced using malt as a raw material, first, other wheat such as malt, if necessary, other grains, starch, sugars, bitters, or raw materials such as colorants and the like. If necessary, an enzyme such as amylase is added to a mixture containing water to cause gelatinization and saccharification, and the mixture is filtered to obtain a saccharified solution. If necessary, hops and bittering agents are added to the saccharified solution and boiled, and solid components such as coagulated proteins are removed in a clarification tank. As an alternative to this saccharified solution, hops may be added to a mixture of malt extract and warm water and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. Known conditions may be used for the conditions such as the saccharification process, the boiling process, and the solid content removal process. Known conditions may be used for the conditions such as fermentation and alcohol storage. In addition, when the utilization of the cyclic dipeptide is caused by yeast, the addition amount can be adjusted as necessary. The fermented liquor obtained is filtered, and carbon dioxide is added to the obtained filtrate. Then, it is filled in a container and subjected to a sterilization process to obtain a target beer-taste beverage. At this time, in order to obtain a more preferable flavor as a beer-taste beverage in each of the above-mentioned steps, addition of aroma components such as fatty acid ester, ethyl acetate and isoamyl acetate may be performed at any step up to filling. In each of the above steps, the cyclic dipeptide may be added in any step up to filling. The cyclic dipeptide can be added preferably by using the heat-treated product of the above-mentioned raw material or a purified product thereof as the cyclic dipeptide-containing component.

  Beer-taste beverages containing alcohol produced without using malt as a raw material, liquid sugar containing a carbon source, nitrogen sources as amino acid-containing materials other than malt or malt, hops, pigments, etc. are mixed with warm water. To make a liquid sugar solution. The liquid sugar solution is boiled. When hops are used as a raw material, the hops may be mixed with the liquid sugar solution during boiling, not before the start of boiling. As an alternative to this saccharified liquid, hops may be added to an extract obtained by adding warm water to an extract using a raw material other than malt and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. Known conditions may be used for the conditions such as fermentation and alcohol storage. When the content of the cyclic dipeptide is changed by yeast to utilize the cyclic dipeptide, the addition amount can be adjusted as necessary. The fermented liquor obtained is filtered, and carbon dioxide is added to the obtained filtrate. Then, it is filled in a container and subjected to a sterilization process to obtain a target beer-taste beverage. At this time, in order to obtain a more preferable flavor as a beer-taste beverage in each of the above-mentioned steps, addition of aroma components such as fatty acid ester, ethyl acetate and isoamyl acetate may be performed at any step up to filling. In each of the above steps, the cyclic dipeptide may be added in any step up to filling. The cyclic dipeptide can be added preferably by using the heat-treated product of the above-mentioned raw material or a purified product thereof as the cyclic dipeptide-containing component.

  The beer-taste beverage that is non-fermented and contains alcohol is not limited to malt, and may be the one in which the alcohol content of the final product is adjusted by adding alcohol for the raw material. The raw material alcohol may be added at any step from the saccharification step to the filling step. In each of the above steps, in order to obtain a more preferable flavor as a beer-taste beverage, addition of aroma components such as fatty acid ester, ethyl acetate, and isoamyl acetate may be performed at any step up to filling. In addition, in each of the above steps, the cyclic dipeptide may be added in any step up to filling. The cyclic dipeptide can be added preferably by using the heat-treated product of the above-mentioned raw material or a purified product thereof as the cyclic dipeptide-containing component.

  An example of the packaged beverage according to the present invention is a non-alcoholic beer-taste beverage. The beverage of the present aspect can be produced in the same manner as a general non-alcoholic beer-taste beverage except that a cyclic dipeptide is used. Below, the manufacturing process of a general non-fermenting non-alcoholic beer taste drink is shown below. By not having a fermentation process using yeast, non-alcoholic beer-taste beverages such as non-alcoholic beer can be easily produced. Common non-fermenting non-alcoholic beer-taste beverages may or may not use malt as a raw material, and can be produced as follows.

  Non-alcoholic beer-taste beverages produced by using malt as a raw material, in addition to wheat such as malt, if necessary, other grains, starch, sugars, bittering agents, or raw materials such as coloring agents and water. If necessary, an enzyme such as amylase is added to the mixture containing the mixture to cause gelatinization and saccharification, and the mixture is filtered to obtain a saccharified solution. If necessary, hops and bittering agents are added to the saccharified solution and boiled, and solid components such as coagulated proteins are removed in a clarification tank. As an alternative to this saccharified solution, hops may be added to a mixture of malt extract and warm water and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. Known conditions may be used for the conditions such as the saccharification process, the boiling process, and the solid content removal process. After boiling, the obtained wort is filtered, and carbon dioxide is added to the obtained filtrate. Then, it is filled in a container and subjected to a sterilization process to obtain a desired non-alcoholic beer-taste beverage. In each of the above steps, in order to obtain a more preferable flavor as a beer-taste beverage, addition of aroma components such as fatty acid ester, ethyl acetate, and isoamyl acetate may be performed at any step up to filling. In each of the above steps, the cyclic dipeptide may be added in any step up to filling. The cyclic dipeptide can be added preferably by using the heat-treated product of the above-mentioned raw material or a purified product thereof as the cyclic dipeptide-containing component.

  When producing a non-alcoholic beer-taste beverage that does not use malt as a raw material, first, liquid sugar containing a carbon source, nitrogen sources as amino acid-containing materials other than malt or malt, hops, pigments, etc. are mixed with warm water. To make a liquid sugar solution. The liquid sugar solution is boiled. When hops are used as a raw material, the hops may be mixed with the liquid sugar solution during boiling, not before the start of boiling. Carbon dioxide is added to the liquid sugar solution after boiling. Then, it is filled in a container and subjected to a sterilization process to obtain a desired non-alcoholic beer-taste beverage. In each of the above steps, in order to obtain a more preferable flavor as a beer-taste beverage, addition of aroma components such as fatty acid ester, ethyl acetate, and isoamyl acetate may be performed at any step up to filling. In each of the above steps, the cyclic dipeptide may be added in any step up to filling. The cyclic dipeptide can be added preferably by using the heat-treated product of the above-mentioned raw material or a purified product thereof as the cyclic dipeptide-containing component.

  The "beer taste drink" in this specification means a carbonated drink having a beer-like flavor. That is, the beer-taste beverages of the present specification include all beer-flavored carbonated beverages unless otherwise specified. Among these, the "non-alcoholic beer-taste beverage" is a beer-taste beverage having an alcohol content of less than 1%, and preferably contains substantially no alcohol. Here, the drink of a mode that does not substantially contain alcohol does not exclude a drink containing an extremely small amount of alcohol that cannot be detected. Beverages having an alcohol content of 0.0% by rounding, particularly, beverages having an alcohol content of 0.00% by rounding are included in non-alcoholic beer-taste beverages. Examples of the beer-taste beverage according to the present embodiment include non-alcoholic beer-taste beverages, beer-taste soft drinks, and the like. The "alcohol content (alcohol content)" here means the content of ethanol and does not include aliphatic alcohol.

  The alcohol content of the beer-taste beverage according to this embodiment means the content (v / v%) of the alcohol content in the beverage, and can be measured by any known method, for example, a vibration type densitometer. Can be measured by Specifically, a sample is prepared by removing carbon dioxide gas from a beverage by filtration or ultrasonic waves, and the sample is subjected to direct flame distillation, and the density of the obtained distillate at 15 ° C. is measured. (Table 19 Alcohol and Density (15 ° C) and Specific Gravity (15/15 ° C) Conversion Table), which is the appendix of (Taira 19 National Tax Agency Instruction No. 6, revised on June 22, 2007) Can be asked. When the alcohol content is a low concentration of less than 1.0%, a commercially available alcohol measuring device or gas chromatography may be used.

  An aliphatic alcohol may be added to the beer-taste beverage according to the present aspect from the viewpoint of imparting a feeling of alcohol. The aliphatic alcohol is not particularly limited as long as it is a known one, but an aliphatic alcohol having 4 to 5 carbon atoms is preferable. In the present embodiment, preferable aliphatic alcohols are those having 4 carbon atoms such as 2-methyl-1-propanol and 1-butanol, and those having 5 carbon atoms are 3-methyl-1-butanol and 1-pen. Examples thereof include tanol and 2-pentanol. These can be used alone or in combination of two or more. The content of the aliphatic alcohol having 4 to 5 carbon atoms is preferably 0.0002 to 0.0007 mass%, more preferably 0.0003 to 0.0006 mass%. In the present specification, the content of the aliphatic alcohol can be measured using a headspace gas chromatographic method.

(calorie)
Among the beer-taste beverages according to this aspect, the non-alcoholic beer-taste beverages preferably have low calories in accordance with recent low-calorie tastes. Therefore, the number of calories in the beer-taste beverage according to this embodiment is preferably less than 5 kcal / 100 mL, more preferably less than 4 kcal / 100 mL, and further preferably less than 3 kcal / 100 mL.

  The number of calories contained in the beer-taste beverage according to the present aspect is basically calculated according to "Analysis method of nutritional components and the like in nutrition labeling standard" published in connection with the Health Promotion Method. That is, in principle, the energy conversion coefficient of each component (protein: 4 kcal / g, lipid: 9 kcal / g, sugar: 4 kcal / g, dietary fiber: 2 kcal / g, alcohol: 7 kcal / g, organic acid: 3 kcal / g). For more details, please refer to “Analysis methods of nutritional components, etc. in nutrition labeling standards”.

  A specific method for measuring the amount of each nutritional component contained in the beer-taste beverage according to the present aspect may be according to various analysis methods described in the health promotion method “about analysis method of nutritional component etc. in nutrition labeling standard”. Alternatively, the amount of heat and / or the amount of each nutritional component can be known by requesting the Japan Food Analysis Center.

(Sugar)
The sugar contained in the beer-taste beverage according to this embodiment means a sugar based on the nutrition labeling standard of food (Ministry of Health, Labor and Welfare Notification No. 176 of 2003). Specifically, sugar refers to foods from which proteins, lipids, dietary fiber, ash, alcohol and water have been removed. Further, the amount of sugar in the food is calculated by subtracting the amounts of protein, lipid, dietary fiber, ash and water from the weight of the food. In this case, the amounts of protein, lipid, dietary fiber, ash and water content are measured by the method listed in the nutrition labeling standard. Specifically, the amount of protein was measured by the nitrogen quantitative conversion method, and the amount of lipid was measured by the ether extraction method, chloroform / methanol mixed liquid extraction method, Gerber method, acid decomposition method or Reese-Gottlieb method, and the amount of dietary fiber. Is measured by high performance liquid chromatography or Prosky method, ash content is measured by magnesium acetate addition ashing method, direct ashing method or sulfuric acid addition ashing method, water content is Karl Fischer method, drying aid Method, reduced pressure superheat drying method, atmospheric pressure heat drying method or plastic film method.

  It is desirable that the beer-taste beverage according to this aspect has a low sugar content in accordance with the recent low sugar taste. Therefore, the content of sugar in the beer-taste beverage according to this embodiment is preferably less than 0.5 g / 100 mL, more preferably 0.4 g / 100 mL or less, and further preferably 0.3 g / 100 mL or less. The lower limit is not particularly set, but is usually about 0.1 g / 100 mL, and may be, for example, 0.15 g / 100 mL or more, or 0.2 g / 100 mL or more.

(Acidulant)
As the acidulant used in the beer-taste beverage according to the present aspect, it is preferable to use one or more acids selected from the group consisting of citric acid, lactic acid, phosphoric acid, and malic acid. In addition, in this embodiment, succinic acid, tartaric acid, fumaric acid, glacial acetic acid, and the like can be used as an acid other than the above acids. These can be used without limitation as long as they are approved to be added to foods. In the present embodiment, it is preferable to use a combination of lactic acid from the viewpoint of appropriately imparting a mellow sourness and phosphoric acid from the viewpoint of appropriately imparting a slightly pungent sourness.

  The content of the acidulant is, in the beer-taste beverage according to the present embodiment, in terms of citric acid, from the viewpoint of imparting a beer-taste feeling, 200 ppm or more is preferable, 550 ppm or more is more preferable, 700 ppm or more is further preferable, and sourness From the viewpoint of, 15000 ppm or less is preferable, 5500 ppm or less is more preferable, and 2000 ppm or less is further preferable. Therefore, in the present aspect, the content of the sour agent is 200 ppm to 15000 ppm, preferably 550 ppm to 5500 ppm, and more preferably 700 ppm to 1500 ppm in a suitable range in terms of citric acid. In the present specification, the amount converted to citric acid is an amount converted from the acidity of each acidulant based on the acidity of citric acid. For example, the amount converted to citric acid corresponding to 100 ppm of lactic acid is 120 ppm, the converted amount of citric acid corresponding to 100 ppm of phosphoric acid is 200 ppm, and the converted amount of citric acid corresponding to 100 ppm of malic acid is 125 ppm.

  The content of the acidulant in the beer-taste beverage refers to that calculated by analysis by high performance liquid chromatography (HPLC) or the like.

(hop)
In the beer-taste beverage according to this aspect, hops can be used as a part of the raw material. Since the flavor tends to be similar to beer, it is desirable to use hops as part of the raw material. When using hops, usual pellet hops, powdered hops, and hop extracts used in the production of beer and the like can be appropriately selected and used according to the desired flavor. In addition, processed hop products such as isolated hops and reduced hops may be used. The hops used in the beer-taste beverage according to this embodiment include these. The amount of hop added is not particularly limited, but is typically about 0.0001 to 1% by weight based on the total amount of the beverage.

(Other raw materials)
Other ingredients may be used for the beer-taste beverage according to this embodiment, if necessary, within a range that does not impair the effects of the present invention. For example, sweeteners (including high-intensity sweeteners), bitters, flavors, yeast extracts, colorants such as caramel pigments, plant-extracted saponin substances such as soybean saponins and quillaja saponins, plant proteins such as corn and soybeans, and Peptide-containing substances, proteinaceous substances such as bovine serum albumin, seasonings such as dietary fiber and amino acids, and antioxidants such as ascorbic acid can be used as necessary as long as the effects of the present invention are not impaired.

  Thus, the beer-taste beverage according to this embodiment is obtained. The pH of the beer-taste beverage according to this aspect is 3.0 to 5.0, preferably 3.5 to 4.5, and more preferably 3.5 to 4. from the viewpoint of improving the flavor of the beverage. It is 0.

  Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not limited to the following examples.

Preparation of Heat-treated Material Various raw materials were heat-treated according to the following heat treatment method. Table 1 shows the results of analysis of the cyclic dipeptide contained in the heat-treated product by the LC-MS / MS method.
For each raw material shown in Table 1, 3 ml of the raw material was added with 15 ml of distilled water, placed in an autoclave (manufactured by Tommy Seiko Co., Ltd.), and subjected to high temperature and high pressure treatment at 135 ° C. for 0.31 MPa for 3 hours. 10 ml of the treated liquid was diluted 50-fold with ultrapure water and subjected to membrane treatment, and then subjected to LC-MS / MS to determine various cyclic dipeptide concentrations. Details of LC-MS / MS processing conditions are as described in the above-mentioned analysis conditions.

Details of various raw materials shown in Table 1 are as follows.
Soybean peptide: High New AM (Fuji Oil Co., Ltd.)
Malt peptide: Enzyme-treated product of crushed European malt Tea peptide: Enzyme-treated product of Ichiban tea leaf (cultivar: Yabukita) from Kagoshima prefecture

<Manufacture of beer-taste beverages>
The beer-taste beverages shown in Table 2 were prepared as follows.

Examples 1 to 4, 8 to 10, Comparative Example 1
Caramel pigment, hops, iso-α-acid, and a heat-treated soybean peptide were added to the wort that had been saccharified and filtered under known general conditions, and the mixture was boiled and solid components such as coagulated proteins were removed in a clarification tank. Acidulants (lactic acid, phosphoric acid) and flavors (fatty acid ester, ethyl acetate) were added and filtered, carbon dioxide was added to the obtained filtrate, and the bottles were filled to obtain a non-alcoholic beer taste beverage using malt. The pH was as shown in Table 2.

Examples 5-7, 11-14
A non-alcoholic beer-taste beverage containing no malt was prepared in the same manner as in Example 1 except that dietary fiber was used instead of wort. The pH was as shown in Table 2.

<Evaluation of flavor>
The beer-taste beverages of Examples and Comparative Examples were evaluated for the sourness, the aftertaste, the effect of improving the flavor, and the overall evaluation by a sensory test using a rating method by 6 expert panelists as follows, The average score was calculated. Here, the flavor improvement effect refers to the degree of flavor improvement based on the evaluation of Comparative Example 1 as 1 point. The average score of the comprehensive evaluation is preferably 2.0 or more, more preferably 3.0 or more. The results are shown in Tables 2-4.
(Strength of sourness)
“Strong” = 5 points, “Slightly strong” = 4 points, “Good” = 3 points, “Slightly weak” = 2 points, “Weak” = 1 point (aftertaste tightness)
"Very" = 5 points, "Yes" = 4 points, "Slightly" = 3 points, "Slightly" = 2 points, "None" = 1 point (flavor improving effect)
"Very" = 5 points, "Yes" = 4 points, "Slightly" = 3 points, "Slightly" = 2 points, "None" = 1 point (Comprehensive evaluation)
“Very good” = 5 points, “Good” = 4 points, “Slightly good” = 3 points, “Normal” = 2 points, “Inferior” = 1 point

  From Examples 1 to 14 and Comparative Example 1, it was found that when the heat-treated soybean peptide was used, the aftertaste was tight and the flavor was good when the pH of the beverage was in the range of 3.0 to 5.0. This effect was the same in the beer-taste beverage containing alcohol by adding neutral spirits to each example.

  INDUSTRIAL APPLICABILITY The packaged beverage of the present invention contains a cyclic dipeptide and has a good flavor, and can provide a new taste as a favorite product.

Claims (5)

A packaged beverage (excluding beer-taste alcoholic beverage) having a cyclic dipeptide content of 500 to 3300 ppm and a pH of 3.0 to 5.0.   The packaged beverage according to claim 1, wherein the cyclic dipeptide is derived from one or more raw materials selected from the group consisting of soybean, tea leaves, malt, and coffee beans.   The packaged beverage according to claim 2, comprising a heat-treated product of a peptide derived from the raw material or a purified product thereof as the cyclic dipeptide-containing component.   The cyclic dipeptide is cyclotryptophanyl tyrosine [Cyclo (Trp-Tyr)], cycloseryl tyrosine [Cyclo (Ser-Tyr)], cycloprolyl tyrosine [Cyclo (Pro-Tyr)], cycloglycyl tyrosine [Cyclo. (Gly-Tyr)], cyclotyrosyl tyrosine [Cyclo (Tyr-Tyr)], cyclophenylalanyl tyrosine [Cyclo (Phe-Tyr)], cycloleucyl tyrosine [Cyclo (Leu-Tyr)], cyclolysyl tyrosine [Cyclo (Lys-Tyr)], cyclohistidyl tyrosine [Cyclo (His-Tyr)], cycloalanyl tyrosine [Cyclo (Ala-Tyr)], cycloglutamyl tyrosine [Cyclo (Glu-Tyr)], cyclotyro Sylvaline [Cyclo (Tyr-Val)], cycloisoleucyl tyrosine [Cyclo (Ile-Tyr)], cyclothreonyl tyrosine [Cyclo (Thr-Tyr)], cycloaspartyl tyrosine [Cyclo (Asp-Tyr)] , Cycloasparaginyl tyrosine [Cyclo (Asn-Tyr)], cycloglutamin One or more tyrosine-containing ring selected from the group consisting of tyrosine [Cyclo (Gln-Tyr)], cycloarginyl tyrosine [Cyclo (Arg-Tyr)], and cyclomethionyl tyrosine [Cyclo (Met-Tyr)] The packaged beverage according to any one of claims 1 to 3, comprising a dipeptide.   The packaged beverage according to claim 4, wherein the content of the tyrosine-containing cyclic dipeptide is 1 to 400 ppm.