JP6616822B2 - バクテリオファージ・ラムダ・インテグラーゼの変異体 - Google Patents

バクテリオファージ・ラムダ・インテグラーゼの変異体 Download PDF

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JP6616822B2
JP6616822B2 JP2017506963A JP2017506963A JP6616822B2 JP 6616822 B2 JP6616822 B2 JP 6616822B2 JP 2017506963 A JP2017506963 A JP 2017506963A JP 2017506963 A JP2017506963 A JP 2017506963A JP 6616822 B2 JP6616822 B2 JP 6616822B2
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nucleic acid
sequence
seq
lambda integrase
amino acid
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JP2017525355A (ja
JP2017525355A5 (cg-RX-API-DMAC7.html
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ガデッシー、ファリッド
シャウ、ジャ・ウェイ
ドローゲ、ピーター
マキジャ、ハルシャ
ビジャヤ・チャンドラ、シュリー・ハルシャ
ピーター、サブリナ
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エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2017506963A 2014-08-08 2015-08-11 バクテリオファージ・ラムダ・インテグラーゼの変異体 Active JP6616822B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1414130.3 2014-08-08
GBGB1414130.3A GB201414130D0 (en) 2014-08-08 2014-08-08 Mutants of the bacteriophage lambda integrase
PCT/SG2015/050255 WO2016022075A1 (en) 2014-08-08 2015-08-11 Mutants of the bacteriophage lambda integrase

Publications (3)

Publication Number Publication Date
JP2017525355A JP2017525355A (ja) 2017-09-07
JP2017525355A5 JP2017525355A5 (cg-RX-API-DMAC7.html) 2018-09-13
JP6616822B2 true JP6616822B2 (ja) 2019-12-04

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JP2017506963A Active JP6616822B2 (ja) 2014-08-08 2015-08-11 バクテリオファージ・ラムダ・インテグラーゼの変異体

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Country Link
US (2) US10344301B2 (cg-RX-API-DMAC7.html)
EP (1) EP3177717A4 (cg-RX-API-DMAC7.html)
JP (1) JP6616822B2 (cg-RX-API-DMAC7.html)
CN (1) CN108064279B (cg-RX-API-DMAC7.html)
GB (1) GB201414130D0 (cg-RX-API-DMAC7.html)
SG (2) SG10202005089XA (cg-RX-API-DMAC7.html)
WO (1) WO2016022075A1 (cg-RX-API-DMAC7.html)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
BR112022001657A2 (pt) 2019-07-29 2022-03-22 Univ Indiana Trustees Polipeptídeo de fusão, sequência de ácidos nucleicos, cassete de expressão, vetor de expressão, célula hospedeira, composição farmacêutica, e, métodos para tratar a ataxia de friedreich e para tratar uma doença associada à ataxia de friedreich
CA3162628A1 (en) 2019-11-25 2021-06-03 Larimar Therapeutics, Inc. Methods for quantifying frataxin activity
CN115484973A (zh) 2020-04-30 2022-12-16 拉利玛生物医药公司 治疗髓磷脂相关疾病和线粒体相关疾病的方法
US12390509B2 (en) 2020-12-12 2025-08-19 Larimar Therapeutics, Inc. Pharmaceutical composition comprising frataxin fusion protein and methods of use thereof
WO2022191779A1 (en) * 2021-03-12 2022-09-15 Nanyang Technological University Method for the production of seamless dna vectors
US20250320526A1 (en) * 2022-05-31 2025-10-16 Nanyang Technological University Method for production of a eukaryotic host cell or cell line for lambda-integrase-mediated recombination
CN118546903A (zh) * 2023-02-24 2024-08-27 尧唐(上海)生物科技有限公司 能介导大片段dna整合到哺乳动物基因组的酶及其应用
WO2024181932A1 (en) * 2023-03-02 2024-09-06 Nanyang Technological University Lambda integrase mutants and methods of their use
WO2025006468A2 (en) 2023-06-26 2025-01-02 University Of Hawaii Evolved integrases and methods of using the same for genome editing

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2522166C (en) 2001-05-30 2011-05-03 Chromos Molecular Systems, Inc. Lambda integrase mutein for use in recombination
US20030119104A1 (en) * 2001-05-30 2003-06-26 Edward Perkins Chromosome-based platforms
EP2362897A2 (en) * 2008-11-11 2011-09-07 Danisco US Inc. Compositions and methods comprising serine protease variants
SG170652A1 (en) * 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
GB201414130D0 (en) * 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase

Also Published As

Publication number Publication date
US11414681B2 (en) 2022-08-16
WO2016022075A1 (en) 2016-02-11
JP2017525355A (ja) 2017-09-07
EP3177717A4 (en) 2017-12-27
CN108064279B (zh) 2022-03-11
US20200063165A1 (en) 2020-02-27
CN108064279A (zh) 2018-05-22
SG11201700955SA (en) 2017-03-30
GB201414130D0 (en) 2014-09-24
SG10202005089XA (en) 2020-07-29
US10344301B2 (en) 2019-07-09
EP3177717A1 (en) 2017-06-14
US20170327847A1 (en) 2017-11-16

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