JP6593876B2 - 尿路上皮癌の検出方法 - Google Patents
尿路上皮癌の検出方法 Download PDFInfo
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- JP6593876B2 JP6593876B2 JP2015518261A JP2015518261A JP6593876B2 JP 6593876 B2 JP6593876 B2 JP 6593876B2 JP 2015518261 A JP2015518261 A JP 2015518261A JP 2015518261 A JP2015518261 A JP 2015518261A JP 6593876 B2 JP6593876 B2 JP 6593876B2
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- JP
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- Prior art keywords
- mir
- urothelial cancer
- cancer
- patients
- expression level
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Description
本発明の一形態によれば、尿検体中のmiR−519aの発現量の変動を測定する工程を含む、尿路上皮癌の検出方法が提供される。
(a)被検物質の存在下に細胞を培養する工程、
(b)前記被検細胞におけるmiR−519aの発現量を測定する工程、および
(c)発現量の亢進を抑制するかまたは発現量を減少させる物質を選択する工程、
を含む方法でスクリーニングすることができる。
外来受診時に肉眼的血尿を呈した患者89名のそれぞれから、尿検体を約10mLずつ採取した。次いで、採取された尿検体を3000rpm×15分間(4℃)遠心分離し、沈渣を約1mL残して上清をデカンテーションにより除去した。そして、後述するRNA抽出処理まで−80℃にて保存した。
Claims (5)
- 肉眼的血尿検体中のmiR−519aの発現量が正常組織由来の細胞の発現量に比べて10倍以上亢進していることを尿路上皮癌の判定基準とする、尿路上皮癌の検出方法。
- miR−519aの発現量の測定を、miR−519aに特異的にハイブリダイズするプライマーを用いて行う、請求項1に記載の尿路上皮癌の検出方法。
- miR−519aの発現量の測定を、リアルタイムPCR法により行う、請求項2に記載の尿路上皮癌の検出方法。
- 尿細胞検査を行う工程をさらに含む、請求項1〜3のいずれか1項に記載の尿路上皮癌の検出方法。
- 請求項1〜4のいずれか1項に記載の方法に用いられるキットであって、miR−519aに特異的にハイブリダイズするプライマーを含有するキット。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2013107956 | 2013-05-22 | ||
JP2013107956 | 2013-05-22 | ||
PCT/JP2014/063368 WO2014189052A1 (ja) | 2013-05-22 | 2014-05-20 | 尿路上皮癌の検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2014189052A1 JPWO2014189052A1 (ja) | 2017-02-23 |
JP6593876B2 true JP6593876B2 (ja) | 2019-10-23 |
Family
ID=51933605
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2015518261A Expired - Fee Related JP6593876B2 (ja) | 2013-05-22 | 2014-05-20 | 尿路上皮癌の検出方法 |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP6593876B2 (ja) |
TW (1) | TW201502277A (ja) |
WO (1) | WO2014189052A1 (ja) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5884219B2 (ja) * | 2009-07-16 | 2016-03-15 | 国立大学法人 千葉大学 | miRNA発現プロファイリングに基づく尿路上皮癌の検出方法 |
-
2014
- 2014-05-20 WO PCT/JP2014/063368 patent/WO2014189052A1/ja active Application Filing
- 2014-05-20 JP JP2015518261A patent/JP6593876B2/ja not_active Expired - Fee Related
- 2014-05-22 TW TW103117876A patent/TW201502277A/zh unknown
Also Published As
Publication number | Publication date |
---|---|
WO2014189052A1 (ja) | 2014-11-27 |
TW201502277A (zh) | 2015-01-16 |
JPWO2014189052A1 (ja) | 2017-02-23 |
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