JP6591898B2 - Ｆａｄ２−１ａ／１ｂ遺伝子の標的化ノックアウトを介した、大豆油組成物の改変 - Google Patents

Ｆａｄ２−１ａ／１ｂ遺伝子の標的化ノックアウトを介した、大豆油組成物の改変 Download PDF

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JP6591898B2
JP6591898B2 JP2015562512A JP2015562512A JP6591898B2 JP 6591898 B2 JP6591898 B2 JP 6591898B2 JP 2015562512 A JP2015562512 A JP 2015562512A JP 2015562512 A JP2015562512 A JP 2015562512A JP 6591898 B2 JP6591898 B2 JP 6591898B2
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fad2
soybean plant
plant
soybean
seq
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JP2016509865A5 (enExample
JP2016509865A (ja
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ルク マチス
ルク マチス
ダニエル エフ． ボイタス
ダニエル エフ． ボイタス
フェン チャン
フェン チャン
ウィリアム ハーン
ウィリアム ハーン
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Cellectis SA
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015562512A 2013-03-15 2014-03-13 Ｆａｄ２−１ａ／１ｂ遺伝子の標的化ノックアウトを介した、大豆油組成物の改変 Active JP6591898B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790655P 2013-03-15 2013-03-15
US61/790,655 2013-03-15
US201361885213P 2013-10-01 2013-10-01
US61/885,213 2013-10-01
PCT/IB2014/059752 WO2014141147A1 (en) 2013-03-15 2014-03-13 Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes

Publications (3)

Publication Number Publication Date
JP2016509865A JP2016509865A (ja) 2016-04-04
JP2016509865A5 JP2016509865A5 (enExample) 2017-04-13
JP6591898B2 true JP6591898B2 (ja) 2019-10-16

Family

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JP2015562512A Active JP6591898B2 (ja) 2013-03-15 2014-03-13 Ｆａｄ２−１ａ／１ｂ遺伝子の標的化ノックアウトを介した、大豆油組成物の改変

Country Status (6)

Country Link
EP (1) EP2966983B1 (enExample)
JP (1) JP6591898B2 (enExample)
CN (1) CN105246324B (enExample)
BR (1) BR112015022778B1 (enExample)
CA (1) CA2906406C (enExample)
WO (1) WO2014141147A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
ES3039237T3 (en) 2016-02-02 2025-10-20 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
EA202091316A1 (ru) 2016-12-22 2020-08-18 Тулджин Инкорпорейтид Обогащенный олеиновой кислотой растительный организм, имеющий генетически модифицированный fad2, и способ его получения
CN108374007B (zh) * 2018-02-03 2021-09-21 吉林省农业科学院 高油酸转基因大豆事件eb8072外源插入片段侧翼序列及其应用
AU2020350760B2 (en) * 2019-09-20 2024-10-31 Cibus Europe B.V. Low phytoestrogen soybean based on gene editing
WO2021108248A1 (en) * 2019-11-27 2021-06-03 Calyxt, Inc. Tal-effector nucleases for gene editing
CN112680465A (zh) * 2020-12-28 2021-04-20 浙江农林大学 用于改造大豆脂肪酸组分的基因突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6414223B1 (en) * 1998-08-03 2002-07-02 Cargill, Incorporated Plants, seeds and oils having an elevated total monounsaturated fatty acid content
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US20060206949A1 (en) * 2003-01-28 2006-09-14 Sylvain Arnould Custom-made meganuclease and use thereof
CN100339481C (zh) * 2005-03-17 2007-09-26 东北师范大学 应用基因沉默技术提高大豆和花生的种子油酸含量的方法
CA2765560C (en) * 2009-06-22 2017-10-31 Saga University Mutation that increases the oleic acid content in soybean oil and responsible gene thereof
CN105557503A (zh) * 2009-07-08 2016-05-11 密苏里大学管委会 利用传统大豆育种技术培育高油酸大豆的方法
CN102782140B (zh) * 2009-10-22 2016-04-13 陶氏农业科学有限公司 靶向参与脂肪酸生物合成的植物基因的工程改造锌指蛋白
PT2816112T (pt) * 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
PY1202285A (es) * 2011-01-14 2015-11-02 Univ Missouri Método para desarrollar granos de soja con alto contenido de ácido oleico usando técnicas convencionales de reproducción de la soja
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив

Also Published As

Publication number Publication date
CA2906406C (en) 2023-03-14
BR112015022778B1 (pt) 2023-04-11
WO2014141147A1 (en) 2014-09-18
EP2966983A1 (en) 2016-01-20
CN105246324A (zh) 2016-01-13
EP2966983B1 (en) 2021-07-28
JP2016509865A (ja) 2016-04-04
CA2906406A1 (en) 2014-09-18
CN105246324B (zh) 2018-08-28
BR112015022778A2 (pt) 2017-11-07

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