CN105246324B - 通过靶向敲除fad2-1a/1b基因来改良大豆油成分 - Google Patents

通过靶向敲除fad2-1a/1b基因来改良大豆油成分 Download PDF

Info

Publication number
CN105246324B
CN105246324B CN201480028282.4A CN201480028282A CN105246324B CN 105246324 B CN105246324 B CN 105246324B CN 201480028282 A CN201480028282 A CN 201480028282A CN 105246324 B CN105246324 B CN 105246324B
Authority
CN
China
Prior art keywords
fad2
plant
soybean
plants
allele
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480028282.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN105246324A (zh
Inventor
L·马西斯
D·F·沃塔斯
张峰
W·豪恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectis SA
Original Assignee
Cellectis SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellectis SA filed Critical Cellectis SA
Publication of CN105246324A publication Critical patent/CN105246324A/zh
Application granted granted Critical
Publication of CN105246324B publication Critical patent/CN105246324B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480028282.4A 2013-03-15 2014-03-13 通过靶向敲除fad2-1a/1b基因来改良大豆油成分 Active CN105246324B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790655P 2013-03-15 2013-03-15
US61/790,655 2013-03-15
US201361885213P 2013-10-01 2013-10-01
US61/885,213 2013-10-01
PCT/IB2014/059752 WO2014141147A1 (en) 2013-03-15 2014-03-13 Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes

Publications (2)

Publication Number Publication Date
CN105246324A CN105246324A (zh) 2016-01-13
CN105246324B true CN105246324B (zh) 2018-08-28

Family

ID=50543262

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480028282.4A Active CN105246324B (zh) 2013-03-15 2014-03-13 通过靶向敲除fad2-1a/1b基因来改良大豆油成分

Country Status (6)

Country Link
EP (1) EP2966983B1 (enExample)
JP (1) JP6591898B2 (enExample)
CN (1) CN105246324B (enExample)
BR (1) BR112015022778B1 (enExample)
CA (1) CA2906406C (enExample)
WO (1) WO2014141147A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
EP4646936A3 (en) 2016-02-02 2025-11-19 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
AU2017379402B2 (en) * 2016-12-22 2022-03-10 Toolgen Incorporated Oleic acid-enriched plant body having genetically modified FAD2 and production method therefor
CN108374007B (zh) * 2018-02-03 2021-09-21 吉林省农业科学院 高油酸转基因大豆事件eb8072外源插入片段侧翼序列及其应用
AU2020350760B2 (en) * 2019-09-20 2024-10-31 Cibus Europe B.V. Low phytoestrogen soybean based on gene editing
AU2020391435A1 (en) * 2019-11-27 2022-06-23 Cibus Europe B.V. Tal-effector nucleases for gene editing
CN112680465A (zh) * 2020-12-28 2021-04-20 浙江农林大学 用于改造大豆脂肪酸组分的基因突变体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724669A (zh) * 2005-03-17 2006-01-25 东北师范大学 应用基因沉默技术提高大豆和花生种子的油酸含量的方法
WO2012106105A1 (en) * 2011-01-14 2012-08-09 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques
CN102782140A (zh) * 2009-10-22 2012-11-14 陶氏农业科学有限公司 靶向参与脂肪酸生物合成的植物基因的工程改造锌指蛋白

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6414223B1 (en) * 1998-08-03 2002-07-02 Cargill, Incorporated Plants, seeds and oils having an elevated total monounsaturated fatty acid content
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
WO2004067753A2 (en) * 2003-01-28 2004-08-12 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof.
BRPI1015426A2 (pt) * 2009-06-22 2016-06-28 Univ Saga planta de soja não-modificada geneticamente, composição farmacêutica, produto alimentício, ou ração, óleo de soja, combustível, métodos para fabricar e para selecionar uma planta de soja não-modificada geneticamente, e para produzir óleo de soja, oligonucleotídeo, microconjunto, e, kit.
US9035129B2 (en) * 2009-07-08 2015-05-19 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques
NO2510096T3 (enExample) 2009-12-10 2015-03-21
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724669A (zh) * 2005-03-17 2006-01-25 东北师范大学 应用基因沉默技术提高大豆和花生种子的油酸含量的方法
CN102782140A (zh) * 2009-10-22 2012-11-14 陶氏农业科学有限公司 靶向参与脂肪酸生物合成的植物基因的工程改造锌指蛋白
WO2012106105A1 (en) * 2011-01-14 2012-08-09 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting;Tomas Cermak等;《Nucleic Acids Research》;20110414;e82:1-11 *
Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait;Anh-Tung Pham等;《BMC Plant Biology》;20100909;195:1-13 *

Also Published As

Publication number Publication date
JP6591898B2 (ja) 2019-10-16
EP2966983B1 (en) 2021-07-28
CA2906406A1 (en) 2014-09-18
WO2014141147A1 (en) 2014-09-18
JP2016509865A (ja) 2016-04-04
EP2966983A1 (en) 2016-01-20
CA2906406C (en) 2023-03-14
BR112015022778B1 (pt) 2023-04-11
CN105246324A (zh) 2016-01-13
BR112015022778A2 (pt) 2017-11-07

Similar Documents

Publication Publication Date Title
Wen et al. TALEN-mediated targeted mutagenesis of fatty acid desaturase 2 (FAD2) in peanut (Arachis hypogaea L.) promotes the accumulation of oleic acid
US10113162B2 (en) Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
CN105246324B (zh) 通过靶向敲除fad2-1a/1b基因来改良大豆油成分
AU2014378946B2 (en) Modified plants
US10487336B2 (en) Methods for selecting plants after genome editing
US20170107527A1 (en) Methods and compositions for use of directed recombination in plant breeding
US20150368659A1 (en) Potatoes with reduced granule-bound starch synthase
US20200149056A1 (en) Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
CN116782762B (zh) 植物单倍体诱导
US20230062896A1 (en) Increase of saturated fat in soybean
AU2019247403B2 (en) FAD2 genes and mutations
WO2025052377A1 (en) Cowpea plants with improved traits and methods for generating the same
WO2023199304A1 (en) Controlling juvenile to reproductive phase transition in tree crops
JP2023526035A (ja) 標的突然変異生成によって変異体植物を得るための方法
CN115667529A (zh) 黄瓜植物习性

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant