BR112015022778B1 - Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja - Google Patents

Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja Download PDF

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Publication number
BR112015022778B1
BR112015022778B1 BR112015022778-3A BR112015022778A BR112015022778B1 BR 112015022778 B1 BR112015022778 B1 BR 112015022778B1 BR 112015022778 A BR112015022778 A BR 112015022778A BR 112015022778 B1 BR112015022778 B1 BR 112015022778B1
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BR
Brazil
Prior art keywords
fad2
soybean
plant
deletion
alleles
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BR112015022778-3A
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English (en)
Portuguese (pt)
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BR112015022778A2 (pt
Inventor
Luc Mathis
Daniel F. Voytas
Feng Zhang
William Haun
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Cellectis
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Publication of BR112015022778A2 publication Critical patent/BR112015022778A2/pt
Publication of BR112015022778B1 publication Critical patent/BR112015022778B1/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112015022778-3A 2013-03-15 2014-03-13 Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja BR112015022778B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790655P 2013-03-15 2013-03-15
US61/790,655 2013-03-15
US201361885213P 2013-10-01 2013-10-01
US61/885,213 2013-10-01
PCT/IB2014/059752 WO2014141147A1 (en) 2013-03-15 2014-03-13 Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes

Publications (2)

Publication Number Publication Date
BR112015022778A2 BR112015022778A2 (pt) 2017-11-07
BR112015022778B1 true BR112015022778B1 (pt) 2023-04-11

Family

ID=50543262

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022778-3A BR112015022778B1 (pt) 2013-03-15 2014-03-13 Método para a produção de uma planta de soja, método para a obtenção de óleo de soja apresentando teor de ácido oleico aumentado e teor de ácido linoleico reduzido e método para gerar uma planta de soja

Country Status (6)

Country Link
EP (1) EP2966983B1 (enExample)
JP (1) JP6591898B2 (enExample)
CN (1) CN105246324B (enExample)
BR (1) BR112015022778B1 (enExample)
CA (1) CA2906406C (enExample)
WO (1) WO2014141147A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
EP4646936A3 (en) * 2016-02-02 2025-11-19 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
US20200208166A1 (en) * 2016-12-22 2020-07-02 Toolgen Incorporated Oleic Acid-Enriched Plant Body Having Genetically Modified FAD2 And Production Method Thereof
CN108374007B (zh) * 2018-02-03 2021-09-21 吉林省农业科学院 高油酸转基因大豆事件eb8072外源插入片段侧翼序列及其应用
EP4030895A1 (en) * 2019-09-20 2022-07-27 Calyxt, Inc. Low phytoestrogen soybean based on gene editing
CA3159737A1 (en) * 2019-11-27 2021-06-03 Zachary DEMOREST Tal-effector nucleases for gene editing
CN112680465A (zh) * 2020-12-28 2021-04-20 浙江农林大学 用于改造大豆脂肪酸组分的基因突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6414223B1 (en) * 1998-08-03 2002-07-02 Cargill, Incorporated Plants, seeds and oils having an elevated total monounsaturated fatty acid content
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
EP1590468A2 (en) 2003-01-28 2005-11-02 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof
CN100339481C (zh) * 2005-03-17 2007-09-26 东北师范大学 应用基因沉默技术提高大豆和花生的种子油酸含量的方法
US9474299B2 (en) * 2009-06-22 2016-10-25 Saga University Mutation that increases the oleic acid content in soybean oil and responsible gene thereof
US9035129B2 (en) * 2009-07-08 2015-05-19 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques
RU2712833C2 (ru) * 2009-10-22 2020-01-31 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот
CA2783351C (en) * 2009-12-10 2021-09-07 Regents Of The University Of Minnesota Tal effector-mediated dna modification
AR085892A1 (es) * 2011-01-14 2013-11-06 Univ Missouri Metodo para desarrollar soja con un contenido elevado de acido oleico usando tecnicas de desarrollo convencionales
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив

Also Published As

Publication number Publication date
BR112015022778A2 (pt) 2017-11-07
JP6591898B2 (ja) 2019-10-16
EP2966983A1 (en) 2016-01-20
CN105246324B (zh) 2018-08-28
CA2906406A1 (en) 2014-09-18
CN105246324A (zh) 2016-01-13
JP2016509865A (ja) 2016-04-04
CA2906406C (en) 2023-03-14
WO2014141147A1 (en) 2014-09-18
EP2966983B1 (en) 2021-07-28

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