JP6564781B2 - 液体サンプル中で細菌を検出し、その濃度を決定するための方法および容器 - Google Patents

液体サンプル中で細菌を検出し、その濃度を決定するための方法および容器 Download PDF

Info

Publication number
JP6564781B2
JP6564781B2 JP2016552205A JP2016552205A JP6564781B2 JP 6564781 B2 JP6564781 B2 JP 6564781B2 JP 2016552205 A JP2016552205 A JP 2016552205A JP 2016552205 A JP2016552205 A JP 2016552205A JP 6564781 B2 JP6564781 B2 JP 6564781B2
Authority
JP
Japan
Prior art keywords
bacteria
liquid sample
volume
container
determining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016552205A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017500884A5 (enExample
JP2017500884A (ja
Inventor
ハモンド，ジェレミー
ラッセル，ジェームス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Idexx Laboratories Inc
Original Assignee
Idexx Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idexx Laboratories Inc filed Critical Idexx Laboratories Inc
Publication of JP2017500884A publication Critical patent/JP2017500884A/ja
Publication of JP2017500884A5 publication Critical patent/JP2017500884A5/ja
Application granted granted Critical
Publication of JP6564781B2 publication Critical patent/JP6564781B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1447—Spatial selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1452—Adjustment of focus; Alignment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2016552205A 2013-10-29 2014-10-28 液体サンプル中で細菌を検出し、その濃度を決定するための方法および容器 Active JP6564781B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361896877P 2013-10-29 2013-10-29
US61/896,877 2013-10-29
PCT/US2014/062624 WO2015066006A2 (en) 2013-10-29 2014-10-28 Method and device for detecting bacteria and determining the concentration thereof in a liquid sample

Publications (3)

Publication Number Publication Date
JP2017500884A JP2017500884A (ja) 2017-01-12
JP2017500884A5 JP2017500884A5 (enExample) 2017-12-07
JP6564781B2 true JP6564781B2 (ja) 2019-08-21

Family

ID=52995866

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016552205A Active JP6564781B2 (ja) 2013-10-29 2014-10-28 液体サンプル中で細菌を検出し、その濃度を決定するための方法および容器

Country Status (7)

Country Link
US (3) US9644230B2 (enExample)
EP (1) EP3063527B1 (enExample)
JP (1) JP6564781B2 (enExample)
CN (1) CN105899933B (enExample)
AU (1) AU2014342581B2 (enExample)
CA (1) CA2928829C (enExample)
WO (1) WO2015066006A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3199937A1 (en) * 2016-01-28 2017-08-02 Minitüb GmbH Counting compartment and method for sample analysis
JP6228282B1 (ja) * 2016-09-27 2017-11-08 株式会社協和医療器 菌培養検査装置及び菌培養検査方法
US11804033B1 (en) * 2017-10-16 2023-10-31 Joseph Bango Microbiological b-captcha to improve artificial intelligence pathogen identification
EP3502661B1 (en) * 2017-12-22 2021-01-20 Minitüb GmbH Method and devices for analyzing sperm samples
EP3791182B1 (en) * 2018-05-09 2024-04-10 F. Hoffmann-La Roche AG Devices and methods for determining particle concentration in a sample
CN110426335B (zh) * 2019-09-05 2022-03-22 苏州大学 一种基于原子力显微镜的纳米颗粒浓度测量方法

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5139031A (en) * 1989-09-18 1992-08-18 La Mina Ltd. Method and device for cytology and microbiological testing
US5329461A (en) 1992-07-23 1994-07-12 Acrogen, Inc. Digital analyte detection system
US5649032A (en) 1994-11-14 1997-07-15 David Sarnoff Research Center, Inc. System for automatically aligning images to form a mosaic image
JPH10161034A (ja) 1996-12-02 1998-06-19 Nikon Corp コンフォーカル顕微鏡及びコンフォーカル顕微鏡を用いた３次元画像の作成方法
EP1935983B1 (en) 1997-05-05 2011-06-22 ChemoMetec A/S Method for determination of biological particles in blood
US6929953B1 (en) 1998-03-07 2005-08-16 Robert A. Levine Apparatus for analyzing biologic fluids
US6313452B1 (en) 1998-06-10 2001-11-06 Sarnoff Corporation Microscopy system utilizing a plurality of images for enhanced image processing capabilities
JP2001095559A (ja) * 1999-09-30 2001-04-10 Japan Bcg Seizo Kk 遠心集菌助剤及び菌回収方法並びに抗酸菌回収方法
TW500920B (en) 2000-03-24 2002-09-01 Olympus Optical Co Defect detecting apparatus
US6711283B1 (en) 2000-05-03 2004-03-23 Aperio Technologies, Inc. Fully automatic rapid microscope slide scanner
US7058233B2 (en) 2001-05-30 2006-06-06 Mitutoyo Corporation Systems and methods for constructing an image having an extended depth of field
US20030103277A1 (en) 2001-12-05 2003-06-05 Mohwinkel Clifford A. Imaging device with angle-compensated focus
JP4896373B2 (ja) 2001-12-28 2012-03-14 ルドルフテクノロジーズ インコーポレイテッド 立体３次元計測システムおよび方法
US7634129B2 (en) 2001-12-28 2009-12-15 Rudolph Technologies, Inc. Dual-axis scanning system and method
US7764821B2 (en) 2002-02-14 2010-07-27 Veridex, Llc Methods and algorithms for cell enumeration in a low-cost cytometer
US6873725B2 (en) 2002-09-09 2005-03-29 Coulter International Corp. Simultaneous measurement and display of 3-D size distributions of particulate materials in suspensions
US7345814B2 (en) 2003-09-29 2008-03-18 Olympus Corporation Microscope system and microscope focus maintaining device for the same
CA2586893A1 (en) * 2004-11-09 2006-05-18 Monogen, Inc. Vial assembly, sampling apparatus and method for processing liquid-based specimens
US7551279B2 (en) * 2005-09-19 2009-06-23 Jmar Technologies, Inc. Systems and methods for detecting normal levels of bacteria in water using a multiple angle light scattering (MALS) instrument
WO2007095090A2 (en) 2006-02-10 2007-08-23 Monogen, Inc. Method and apparatus and computer program product for collecting digital image data from microscope media-based specimens
JP4878913B2 (ja) 2006-05-24 2012-02-15 オリンパス株式会社 顕微鏡システム、顕微鏡画像の合成方法、及びプログラム
US7865007B2 (en) 2006-10-30 2011-01-04 Olympus Corporation Microscope system, observation method and observation program
US7576307B2 (en) 2007-04-30 2009-08-18 General Electric Company Microscope with dual image sensors for rapid autofocusing
WO2008137746A1 (en) 2007-05-04 2008-11-13 Aperio Technologies, Inc. Rapid microscope scanner for volume image acquisition
US7630628B2 (en) 2007-07-27 2009-12-08 Nikon Corporation Microscope system and microscope observation method
JP5068121B2 (ja) 2007-08-27 2012-11-07 株式会社ミツトヨ 顕微鏡および三次元情報取得方法
JP5100360B2 (ja) 2007-12-21 2012-12-19 株式会社トプコン 画像処理装置
EP2225598A1 (en) 2007-12-21 2010-09-08 Koninklijke Philips Electronics N.V. Scanning microscope and method of imaging a sample.
US20090185734A1 (en) * 2008-01-18 2009-07-23 Hemocue Ab Apparatus and method for analysis of particles in a liquid sample
WO2009100197A2 (en) * 2008-02-05 2009-08-13 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
WO2009124179A1 (en) 2008-04-02 2009-10-08 Abbott Point Of Care, Inc. Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood
DK200801722A (en) 2008-12-05 2010-06-06 Unisensor As Optical sectioning of a sample and detection of particles in a sample
US8309897B2 (en) * 2009-02-06 2012-11-13 Pocared Diagnostics Ltd. Optical measurement arrangement
US8149401B2 (en) 2009-02-13 2012-04-03 Velcon Filters, Llc System and method for distinguishing particles in a transient fluid
KR20120089769A (ko) 2009-12-04 2012-08-13 유니센서 에이/에스 생물학적 유기체의 시간-관련 현미경 검사용 시스템 및 방법
WO2011072698A1 (en) * 2009-12-18 2011-06-23 Unisensor A/S Stroboscopic counting of particles
EP2612901A4 (en) 2010-08-30 2017-05-17 Panasonic Healthcare Holdings Co., Ltd. Microbial counting cell, microbe counting device using same, and microbe counting method using same

Also Published As

Publication number Publication date
EP3063527A2 (en) 2016-09-07
CA2928829A1 (en) 2015-05-07
CN105899933B (zh) 2020-03-03
EP3063527A4 (en) 2017-10-18
EP3063527B1 (en) 2020-12-02
US10359352B2 (en) 2019-07-23
US20190285535A1 (en) 2019-09-19
WO2015066006A3 (en) 2015-06-25
AU2014342581A1 (en) 2016-06-16
US10914670B2 (en) 2021-02-09
CA2928829C (en) 2021-10-26
AU2014342581B2 (en) 2020-01-16
CN105899933A (zh) 2016-08-24
US9644230B2 (en) 2017-05-09
WO2015066006A2 (en) 2015-05-07
US20150118708A1 (en) 2015-04-30
US20170212032A1 (en) 2017-07-27
JP2017500884A (ja) 2017-01-12

Similar Documents

Publication Publication Date Title
JP6564781B2 (ja) 液体サンプル中で細菌を検出し、その濃度を決定するための方法および容器
JP7361149B2 (ja) デジタルホログラフィ顕微鏡検査および無傷の（ｕｎｔｏｕｃｈｅｄ）末梢血白血球を用いる高精度の５部鑑別（５－ｐａｒｔ Ｄｉｆｆｅｒｅｎｔｉａｌ）
US9904842B2 (en) System and method for classification of particles in a fluid sample
Nawaz et al. Sub-micrometer-precision, three-dimensional (3D) hydrodynamic focusing via “microfluidic drifting”
CN113215221A (zh) 用于使用多分散小滴执行数字检定的方法和设备
CN104390895B (zh) 基于显微成像的图像灰度测量颗粒粒径的方法
US20190234853A1 (en) Holographic characterization using hu moments
CN102753954A (zh) 利用光学力将胶粒分成多个通道：棱镜光学分离
DE112013001375T5 (de) Durchflusszelle, Analysevorrichtung und Analyseverfahren unter Verwendung derselben
Hajjoul et al. Lab-on-Chip for fast 3D particle tracking in living cells
Frisk et al. A silicon-glass microwell platform for high-resolution imaging and high-content screening with single cell resolution
JP2017026527A (ja) 凝集沈殿制御装置、凝集沈殿制御方法、コンピュータプログラム及び凝集沈殿システム
US12233418B2 (en) Cell capture in microfluidic devices
CN114870913A (zh) 一种集成弹性-惯性聚焦和虚拟流道的微流控器件及系统
US20220299420A1 (en) Systems and methods for image cytometry
US12290814B2 (en) Bubble discharging method, particle trapping apparatus, and particle analyzing apparatus
KR102072445B1 (ko) 마이크로웰 어레이의 이미지 분석을 위한 분석 알고리즘 형성방법
US11525770B2 (en) Process and system for sizing two-dimensional nanostructures
JP7622231B2 (ja) 画像平面分析を伴う血液分析器および関連する方法
US20250182274A1 (en) Quantifying constituents in a sample chamber using images of depth regions
Yaghoobi et al. Progressive Sperm Separation Using Parallelized, High-Throughput, Microchamber-based Microfluidics
WO2025101870A1 (en) Systems and methods for biological optical imaging with artificial particles as references
Kongsaktrakul Using obstacle trenches for enhancing microplastic trapping in microwells
CA3252876A1 (en) METHOD FOR DETERMINING BACTERIA IN SAMPLES
JP2025502722A (ja) 光設定に基づく細胞分類による生体液分析器

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171026

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20171026

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20180823

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20181002

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181219

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190215

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190723

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190729

R150 Certificate of patent or registration of utility model

Ref document number: 6564781

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250