CA2928829C - Method and device for detecting bacteria and determining the concentration thereof in a liquid sample - Google Patents
Method and device for detecting bacteria and determining the concentration thereof in a liquid sample Download PDFInfo
- Publication number
- CA2928829C CA2928829C CA2928829A CA2928829A CA2928829C CA 2928829 C CA2928829 C CA 2928829C CA 2928829 A CA2928829 A CA 2928829A CA 2928829 A CA2928829 A CA 2928829A CA 2928829 C CA2928829 C CA 2928829C
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- CA
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- Prior art keywords
- bacteria
- liquid sample
- volume
- container
- determining
- Prior art date
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/493—Physical analysis of biological material of liquid biological material urine
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/1447—Spatial selection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/1452—Adjustment of focus; Alignment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Clinical Laboratory Science (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361896877P | 2013-10-29 | 2013-10-29 | |
| US61/896,877 | 2013-10-29 | ||
| PCT/US2014/062624 WO2015066006A2 (en) | 2013-10-29 | 2014-10-28 | Method and device for detecting bacteria and determining the concentration thereof in a liquid sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2928829A1 CA2928829A1 (en) | 2015-05-07 |
| CA2928829C true CA2928829C (en) | 2021-10-26 |
Family
ID=52995866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2928829A Active CA2928829C (en) | 2013-10-29 | 2014-10-28 | Method and device for detecting bacteria and determining the concentration thereof in a liquid sample |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US9644230B2 (enExample) |
| EP (1) | EP3063527B1 (enExample) |
| JP (1) | JP6564781B2 (enExample) |
| CN (1) | CN105899933B (enExample) |
| AU (1) | AU2014342581B2 (enExample) |
| CA (1) | CA2928829C (enExample) |
| WO (1) | WO2015066006A2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3199937A1 (en) * | 2016-01-28 | 2017-08-02 | Minitüb GmbH | Counting compartment and method for sample analysis |
| JP6228282B1 (ja) * | 2016-09-27 | 2017-11-08 | 株式会社協和医療器 | 菌培養検査装置及び菌培養検査方法 |
| US11804033B1 (en) * | 2017-10-16 | 2023-10-31 | Joseph Bango | Microbiological b-captcha to improve artificial intelligence pathogen identification |
| EP3502661B1 (en) * | 2017-12-22 | 2021-01-20 | Minitüb GmbH | Method and devices for analyzing sperm samples |
| EP3791182B1 (en) * | 2018-05-09 | 2024-04-10 | F. Hoffmann-La Roche AG | Devices and methods for determining particle concentration in a sample |
| CN110426335B (zh) * | 2019-09-05 | 2022-03-22 | 苏州大学 | 一种基于原子力显微镜的纳米颗粒浓度测量方法 |
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| US5329461A (en) | 1992-07-23 | 1994-07-12 | Acrogen, Inc. | Digital analyte detection system |
| US5649032A (en) | 1994-11-14 | 1997-07-15 | David Sarnoff Research Center, Inc. | System for automatically aligning images to form a mosaic image |
| JPH10161034A (ja) | 1996-12-02 | 1998-06-19 | Nikon Corp | コンフォーカル顕微鏡及びコンフォーカル顕微鏡を用いた３次元画像の作成方法 |
| EP1935983B1 (en) | 1997-05-05 | 2011-06-22 | ChemoMetec A/S | Method for determination of biological particles in blood |
| US6929953B1 (en) | 1998-03-07 | 2005-08-16 | Robert A. Levine | Apparatus for analyzing biologic fluids |
| US6313452B1 (en) | 1998-06-10 | 2001-11-06 | Sarnoff Corporation | Microscopy system utilizing a plurality of images for enhanced image processing capabilities |
| JP2001095559A (ja) * | 1999-09-30 | 2001-04-10 | Japan Bcg Seizo Kk | 遠心集菌助剤及び菌回収方法並びに抗酸菌回収方法 |
| TW500920B (en) | 2000-03-24 | 2002-09-01 | Olympus Optical Co | Defect detecting apparatus |
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| JP4896373B2 (ja) | 2001-12-28 | 2012-03-14 | ルドルフテクノロジーズ インコーポレイテッド | 立体３次元計測システムおよび方法 |
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| WO2009100197A2 (en) * | 2008-02-05 | 2009-08-13 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| WO2009124179A1 (en) | 2008-04-02 | 2009-10-08 | Abbott Point Of Care, Inc. | Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood |
| DK200801722A (en) | 2008-12-05 | 2010-06-06 | Unisensor As | Optical sectioning of a sample and detection of particles in a sample |
| US8309897B2 (en) * | 2009-02-06 | 2012-11-13 | Pocared Diagnostics Ltd. | Optical measurement arrangement |
| US8149401B2 (en) | 2009-02-13 | 2012-04-03 | Velcon Filters, Llc | System and method for distinguishing particles in a transient fluid |
| KR20120089769A (ko) | 2009-12-04 | 2012-08-13 | 유니센서 에이/에스 | 생물학적 유기체의 시간-관련 현미경 검사용 시스템 및 방법 |
| WO2011072698A1 (en) * | 2009-12-18 | 2011-06-23 | Unisensor A/S | Stroboscopic counting of particles |
| EP2612901A4 (en) | 2010-08-30 | 2017-05-17 | Panasonic Healthcare Holdings Co., Ltd. | Microbial counting cell, microbe counting device using same, and microbe counting method using same |
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2014
- 2014-10-28 AU AU2014342581A patent/AU2014342581B2/en active Active
- 2014-10-28 EP EP14858727.2A patent/EP3063527B1/en active Active
- 2014-10-28 CA CA2928829A patent/CA2928829C/en active Active
- 2014-10-28 WO PCT/US2014/062624 patent/WO2015066006A2/en not_active Ceased
- 2014-10-28 JP JP2016552205A patent/JP6564781B2/ja active Active
- 2014-10-28 US US14/525,628 patent/US9644230B2/en active Active
- 2014-10-28 CN CN201480071692.7A patent/CN105899933B/zh active Active
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2017
- 2017-03-16 US US15/461,087 patent/US10359352B2/en active Active
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2019
- 2019-05-23 US US16/421,054 patent/US10914670B2/en active Active
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| Publication number | Publication date |
|---|---|
| JP6564781B2 (ja) | 2019-08-21 |
| EP3063527A2 (en) | 2016-09-07 |
| CA2928829A1 (en) | 2015-05-07 |
| CN105899933B (zh) | 2020-03-03 |
| EP3063527A4 (en) | 2017-10-18 |
| EP3063527B1 (en) | 2020-12-02 |
| US10359352B2 (en) | 2019-07-23 |
| US20190285535A1 (en) | 2019-09-19 |
| WO2015066006A3 (en) | 2015-06-25 |
| AU2014342581A1 (en) | 2016-06-16 |
| US10914670B2 (en) | 2021-02-09 |
| AU2014342581B2 (en) | 2020-01-16 |
| CN105899933A (zh) | 2016-08-24 |
| US9644230B2 (en) | 2017-05-09 |
| WO2015066006A2 (en) | 2015-05-07 |
| US20150118708A1 (en) | 2015-04-30 |
| US20170212032A1 (en) | 2017-07-27 |
| JP2017500884A (ja) | 2017-01-12 |
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