JP6538304B2 - Water purification method in closed water system using microorganisms - Google Patents

Description

  The present invention relates to a water purification method using microorganisms in a closed water system such as a golf course pond or pool.

  In closed water systems such as golf course ponds and garden ponds, algae and the like grow especially in summer, the water turns green, the water quality deteriorates, and a bow hula etc. is generated. It is easy to be in a state of absence. In addition, in the reservoir, algae may grow to the surface of the water, and it may not be possible to use the water at an emergency.

Also, in the pool, even if summer is over and the season is off, it is usual that water is stored without draining for disaster prevention. Before the next season's pool opens, flush the stored water and clean the inside of the pool, and then put in new water and use the pool.
However, in the water of the pool off the season, many algae are generated and propagated, clinging to the wall of the pool and damaging the wall. Therefore, it is not only unfavorable in terms of hygiene, disaster prevention, environment, scenery, etc., but it is slippery and dangerous when it is cleaned, and there is a problem that time, labor, and cost required for cleaning become large.

In order to solve such a problem, Patent Document 1 discloses a method for preventing algae or the like generated in a pool by a liquid complex enzyme.
However, this method has a problem that it requires a large amount of labor and cost because "the predetermined amount has to be input once a week".

In Patent Document 2, organisms in water such as algae in water are killed by a biological control agent in water such as copper sulfate and benzalkonium chloride, and the organisms suspended in water suspended are precipitated and aggregated using a sedimentation agent and a flocculant. Discloses a method of purifying water by decomposing it by microorganisms. This method is intended to remove floating aquatic organisms killed by the aquatic bioremoving agent, which conventionally took time to remove (paragraphs (0010) and (0011) of Patent Document 2). etc).
However, this method usually needs to generate a stream of water in the water to accelerate the action (claim 3, paragraph (0038), etc. of Patent Document 2), and in addition to microorganisms, aquatic biocontrol agents and flocculants There is a problem that it takes time and cost to use.

In Patent Document 3, a liquid containing algae is brought into contact with a water-containing gel carrier in which an algal microorganism of Brevibacillus type is entrapped and fixed with a polymer material, and then microcystin released by the decomposition of the algae is obtained. A process for treating algae and microcystins is described, which is contacted with a carrier carrying Sphingomonas bacteria. The method described in this document is a treatment method in which the entrapped and immobilized carrier is added to the closed water system to kill planktonic blue-green algae and to harm the harmful components generated by the alga.
However, this method can not cope with green algae and the like, and there is a problem that the preparation of the carrier and the subsequent processing such as removal are laborious.

Japanese Patent Application Laid-Open No. 10-263567 Japanese Patent Application Laid-Open No. 11-156383 JP, 2004-81926, A

  The present invention has been made in view of the above-described conventional technology, and economically and safely and easily suppresses the reproduction of algae such as green algae, cyanobacteria, diatoms and the like in a closed water system to purify the water quality. The task is to provide a way to

The present inventors diligently studied to solve the above problems. As a result, it is a microbial preparation mainly composed of dried powder grains in which natural plants are fermented with Bacillus subtilis in a closed water system such as a golf course pond, and the number of cells is 1 × 10 ⁹ cells / g or more, and average grain size what is diameter 0.8mm or less, when introduced at a rate of 100~10,000g per water 300 meters ^3, in the closed water system, green algae, cyanobacteria, algae growth of diatoms like, economical, safe and It has been found that it is possible to easily suppress and purify the water quality, and the present invention has been completed.

Thus, according to the present invention, there is provided a method of water purification in a closed water system of the following (1) to (7).
(1) A microorganism preparation comprising mainly dry powder obtained by fermenting natural plants with Bacillus subtilis in a closed water system, wherein the number of bacteria is 1 × 10 ⁹ cells / g or more, and the average particle diameter is 0. what is 8mm or less, characterized in that it is turned at a rate of 100~10,000g per water 300 meters ^3, water purification method in a closed water system.
(2) The method for water purification in a closed water system according to (1), characterized in that the microbial preparation is introduced at least once between March and June or September and December.
(3) The method for water purification in a closed water system according to (1) or (2), characterized in that the microbial preparation is enclosed in a water-soluble film bag and introduced.
(4) The method for water purification in a closed water system according to any one of (1) to (3), wherein the maximum water depth of the closed water system is 2 m or less.

(5) In the closed water system according to any one of (1) to (4), the closed water system is a golf course pond, a garden pond, a dam, a reservoir, a reservoir, a pool, or a swamp. Water purification method.
(6) Any of the above (1) to (5), characterized in that the above-mentioned closed water system is a golf course pond, and the above-mentioned microorganism preparation is injected at least once between September and November or March and May. A method for water purification in a closed water system as described in Crab.
(7) The closed water system is an off-season outdoor pool, and the microbial preparation is injected at least once between September and November, and is further injected every one to two months until next season's pool cleaning. The water purification method in the closed water system according to any one of (1) to (5), characterized in that

According to the present invention, the reproduction of algae such as green algae, cyanobacteria, diatoms and the like in a closed water system can be economically and safely suppressed simply and the water quality can be purified. Therefore, the closed water system can be maintained in a favorable state in terms of sanitation, disaster prevention, environment, and scenery, in which clean water without any occurrence of a bow hula or the like is retained.
The method of the present invention only needs to feed the microorganism preparation into the closed water system, and the operation is simple and economical. When the maximum water depth of the closed water system is 2 m or less, it is not necessary to install an aeration device, a stirring device or the like to aeration or agitation.
The microorganism preparation used in the present invention does not need to be supported by cement or the like, and can be placed in a bag of a water-soluble film so that the whole bag can be charged.
The microorganism preparation used in the present invention is different from conventional water purification chemicals including sala powder, organic sala powder, etc., and herbicides, etc., and the effect does not weaken even if it rains. It is economical because there is no need to use it.

In addition, if the method of the present invention is applied to an outdoor pool that has been flooded for disaster prevention after the pool season is over, the pool water can be kept clean during the off-season. And, in the pool cleaning to be performed before the next season, since algae does not stick to the wall of the pool and it is hard to come off, it is not necessary to strongly rub the pool wall, cleaning the wall is easy without cleaning the wall. , Cleaning costs are reduced.
In addition, since the microorganism preparation used is harmless to humans and animals, it is not necessary to use a filter or the like when removing water, and it is environmentally friendly, economical, safe and simple.

It is a schematic diagram explaining that Bacillus subtilis adheres around algae and forms a floc, and suppresses the reproduction of algae. FIG. 2 is a photomicrograph of water of a golf course pond A of Example 1; FIG. 2 is a photomicrograph of water of a golf course pond A of Example 1; FIG. 16 is a micrograph of water of a golf course pond C of Example 2. FIG. 16 is a micrograph of water of a golf course pond C of Example 2. FIG. 16 is a micrograph of water of a golf course pond C of Example 2. FIG. 16 is a micrograph of water of a golf course pond C of Example 2. FIG. 16 is an overview photograph of a golf course pond D of Example 3. FIG. 16 is an overview photograph of a golf course pond D of Example 3. FIG. 16 is an overview photograph of a golf course pond D of Example 3. FIG. 16 is a photomicrograph of the water of the pool A of school A in Example 4; FIG. 16 is a photomicrograph of the water of the pool A of school A in Example 4; FIG. 16 is a photomicrograph of the water of the pool A of school A in Example 4; FIG. 16 is a photomicrograph of the water of the pool A of school A in Example 4;

The method for purifying water in the closed water system of the present invention is a microbial preparation mainly comprising dried powder particles obtained by fermenting a natural plant with Bacillus subtilis in the closed water system, wherein the number of bacterial cells is 1 × 10 ⁹ cells / g or more. the average particle diameter is 0.8mm or less, characterized in that it is turned at a rate of 100~10,000g per water 300 meters ^3.
That is, the water purification method of the present invention is capable of effectively suppressing the reproduction of algae such as green algae, diatoms and blue algae in a closed water system by administering a predetermined amount of a specific microorganism preparation to the closed water system. , It is a method to purify the water of closed water system efficiently.
Hereinafter, the present invention will be described in detail.

(1) Closed Water System As a closed water system targeted by the water purification method of the present invention, there is no particular limitation as long as it is a closed water system. For example, golf course ponds, garden ponds, dams, reservoirs, reservoirs, pools, marshes, reservoirs, lakes and the like can be mentioned. Among these, when it is a golf course pond and outdoor pool which require cleaning especially, the outstanding effect of the present invention can be acquired.
The accumulated water may be fresh water or seawater.

The water depth of the closed water system is not particularly limited, but the maximum water depth is preferably 2 m or less, more preferably 0.2 to 1.5 m, because the excellent effect of the present invention is easily obtained. preferable. When the water depth is such, even if the closed water system is not aerated, stirred or the like by an aeration device, a stirring device or the like, reproduction of algae can be effectively suppressed and the water quality can be purified.
If the water depth of the closed water system is too small, the wind may wind up the bottom of the sludge and the visibility may deteriorate. On the other hand, when the water depth is large, the microorganism preparation to be used contains aerobic microorganisms (B. subtilis), so that the microorganism preparation is activated using an aeration device or a stirring device to spread over the entire range in the closed water system. Is preferred.

  The pH of the water of the closed water system is preferably in the neutral range (5.8 to 8.6), and is preferably 6.0 to 8.0, because the superior effect of the present invention can be obtained. More preferable.

  Examples of algae that propagate in the closed water system include green algae such as Chlamydomonas, icadamo, closteria, Kunshomo, Donariella, etc .; And the like.

These algae breed by photosynthesis in a closed water area using a slight soil as a nutrient source. Water turns green, transparency decreases and water quality deteriorates.
If it is a pool or a fire extinguishing tank, algae adheres to the wall surface, and when there is no space to adhere, it will form a large aggregate alone or float and float on the water surface, worsening the landscape and reducing the function as water for disaster prevention Let

(2) Microbial preparation Microbial preparation used in the present invention is a seed propagation agent for activated sludge mainly composed of dried powder grains obtained by fermenting natural plants with Bacillus subtilis.
Examples of natural plants include grains obtained from grains or grains such as corn flour, wheat flour, bran, soybean meal, rice bran and the like. In the case of fermentation, phosphates such as ammonium phosphate and inorganic salts such as calcium carbonate can be added.

The number of cells contained in the microbial preparation is preferably as large as possible in order to obtain the excellent effect of the present invention, and specifically, 1 × 10 ⁹ cells / g or more, preferably 1 × 10 ¹⁰ cells / g or more Preferably, it is 1.5 × 10 ¹⁰ pieces / g or more.
The average particle size of the microbial preparation is usually 0.8 mm or less, preferably 0.1 to 0.5 mm, from the viewpoint that the effects of the present invention are easily exhibited.

  As a seed planting agent of the activated sludge which mainly made the dry powder which fermented such a natural plant with Bacillus subtilis, a brand name "Pound salon" (made by Towa Enzyme Co., Ltd.) etc. are mentioned, for example.

  Bacillus subtilis in the microorganism preparation used in the present invention is the same as Bacillus natto and the like, is safe, and does not adversely affect the ecosystem. In addition, Bacillus subtilis in the microorganism preparation used in the present invention ingests and grows more nutrients earlier than algae. Therefore, as shown in FIG. 1 (a), when the microorganism preparation is administered to the place where algae 1 is propagating, Bacillus subtilis contained in the microorganism preparation adheres to the periphery of the algae as shown in FIG. 1 (b) By forming floc 2, nutrients necessary for algae to grow can be covered with algae, and as a result, algae growth can be suppressed and the water quality can be purified.

  In addition, in the closed water system, when the propagation of Bacillus subtilis in the microbial preparation is predominantly maintained with respect to the algae, the algae is mixed in the microbial community (a biofilm or floc formed by Bacillus subtilis) to form a colloid. It will only exist. Therefore, for example, when the closed water system is a pond on a golf course or the like, the cleaning operation is facilitated, and the transparency (transparency) of water is also increased. In addition, when the closed water system is a pool etc., it becomes very difficult to peel off when algae reproduces and adheres to the wall surface, but if it is a colloidal biofilm etc, it will easily peel off even if it adheres to the wall surface Yes, it will be very easy to clean.

(3) Feeding method There is no particular limitation on the method for feeding the above-mentioned microorganism preparation, but in the present invention, it is preferable to enclose the microorganism preparation in a water-soluble film bag and to be fed because it is simple.

The water-soluble film bag is not particularly limited as long as it can be rapidly dissolved in water and the contents can be released into the water without adversely affecting the environment.
Examples of the material of the water-soluble film include polyvinyl alcohol, polyoxypolyalkylene glycol, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, sodium carboxymethyl cellulose, sodium polyacrylate, alginic acid, gelatin, pullulan, solubilized starch, gasoline, Examples include water-soluble paper and water-disintegrable paper. Among these, from the viewpoint of workability, thermoplastic resins such as polyvinyl alcohol are preferable.
Moreover, the thickness of the film is not particularly limited, but generally 20 μm to 100 μm.
In addition, you may make multiple packaging using the water soluble film of the same or different composition as needed.
After the microbial preparation used in the present invention is filled in a water-soluble film bag, the inlet portion may be sealed by pasting or heat sealing.
The enclosed amount of one bag is not particularly limited, but is 100 to 1000 g, preferably 200 to 600 g, from the viewpoint of easy handling, working efficiency, and the like.

Input of the closed water system of the microorganism formulation, cell number per unit weight, of water in the state of closing an aqueous environment, time, purpose, although depending on the closing number of times, usually 100 per water 300 meters ³ is 10,000 g, preferably a 500~2000g per water 300 meters ^3, more preferably 600~900g per water 300 meters ^3.

  The introduction timing of the microbial preparation may be appropriately determined depending on the type of the closed water system to be introduced. Generally, it is preferable to inject at least once between March and June or September and December, more preferably at least once between March and May or September and November, and more preferably, between March and May. And it is more preferable to inject at least once during September and November.

When the microbial preparation is administered a plurality of times, the input interval is usually every two weeks to eight months, preferably every one to six months.
The timing of additional input of the microbial preparation can be determined appropriately according to the weather conditions such as temperature, rainfall, wind, sunshine time; time; surrounding environment; and type, shape, depth of water, maintenance status of closed water system, etc. Just do it.
In addition, unlike a normal drug, the microbial preparation is not diluted by rain (the concentration of the drug is reduced) and the effect is not weakened.

Generally, the temperature rises from June to September when the temperature is high, so a large amount of algae is generated, and it is actively active to increase power. Therefore, a higher water purification effect can be obtained by injecting at least once before the summer when the algae power is still weak or after the summer when the algae power weakens.
In addition, when the microbial preparation is introduced in June-September, in order to exert the control effect, it is preferable to introduce a larger amount than usual.

  September-October is the time when there is a lot of rain and the amount of dissolved oxygen in the water increases, and the inflow of BOD source by rain water makes Bacillus subtilis active. Therefore, at such time, the input amount of the microbial preparation may be smaller.

  In November and February, the water temperature decreases and the algae reproduction decreases, so the water quality is improved and the need for additional input of the microbial preparation is reduced. However, Bacillus subtilis in the microbial preparation has no nutrient source such as algae, and the number of bacteria decreases due to autolysis without the influx of organic substances due to rain etc., which may require additional feeding. is there.

  From the end of March, when the temperature of the algae increases and the inflow of rainwater increases the amount of algae reproduction and the water quality tends to deteriorate, it is preferable to introduce the microbial preparation at this time.

  From the above viewpoints, the water surface of the closed water system is visually observed, and the addition time, input interval, and input amount of the microbial preparation may be determined empirically based on the decrease in transparency and the like.

For example, when the closed water system is a golf course pond, it is preferable to introduce at least once in September to November or March to May, and then to introduce 2 to 4 times a year according to the weather. .
According to such an input method, the landscape of the golf course pond can be maintained and a favorable environment can be maintained at all times.

In addition, when the closed water system is a pool off season, the above-mentioned microorganism preparation is kept in a pool for disaster prevention during the period from September to November when the pool entered a low season. Preferably at least once, and then every 1 to 3 months until next season's pool cleaning.
According to such a feeding method, it is possible to make the next season's pool cleaning very easy because algae does not grow and stick to the pool wall surface.

  According to the water purification method of the present invention, the reproduction of algae is suppressed, the pH is well maintained in the neutral region, the transparency is increased, and the water quality (COD value, BOD value) is well maintained.

Hereinafter, the present invention will be described in more detail by way of examples. However, the present invention is not limited to these examples.
In the examples, the transparency, the COD value, and the BOD value are as follows.
[COD value]
The COD value (chemical oxygen demand) is determined by oxidizing the amount of oxidizable substance in the sample water with an oxidizing agent (potassium permanganate at 100 ° C.) under certain conditions, from the amount of oxidizing agent used. The amount of oxygen required for the Usually, the higher the content of organic matter, the higher the water quality, the higher the value (in the following table, described as "COD _Mn ").

[BOD value]
The BOD value (biochemical oxygen demand) is the amount of oxygen consumed by the growth of aerobic microorganisms in water, respiration, etc. The sample is diluted with dilution water and dissolved when cultured at 20 ° C for 5 days It is determined by measuring the amount of reduction of oxygen. Usually, the higher the quality of water, the higher the quality of water, the higher the organic substance (in the table below, it is described as "BOD ₅ ").

[Perspective degree]
For the transparency, a sample is placed in a transparent cylindrical tube, and a handle is attached to a white marking plate with a cross (double cross) with a thickness of 0.5 mm and a double line with a spacing of 1 mm. Is placed on the bottom of the cylindrical tube, and the double cross described on the white marking substrate of the fluoroscope when observed visually from the top while gradually moving the fluorometer upward. The height (cm) from the bottom of the water layer was measured when it was clearly discernible for the first time, and this value was taken as transparency (cm).

Example 1
In the middle of May, the water of pond A (300 m ² , depth of about 50 cm) and pond B (2000 m ² , depth of about 100 cm) of golf course A was observed.
The surface of the pond A had a light green tea color, and the transparency of the collected water was 34 cm. The water surface of the pond B had a pale bluish green color, and the transparency of the collected water was 36 cm.
Furthermore, the pH value, COD value, and BOD value of the collected water were measured for both ponds. The measurement results are shown in Table 1 below.

Next, add a 200 g water-soluble film (made of polyvinyl alcohol) bag of “pond salon” (made by Towa Enzyme Co., Ltd.), which is a microbial preparation mainly composed of dried powder grains fermented with Bacillus subtilis to a pond A One bag and 12 bags (total 2400 g) were loaded into the pond B, respectively.
Thereafter, observation was performed at the timing shown in Table 1 below, and the fluoroscopy degree, the pH value of the collected water, the COD value, and the BOD value were measured. The measurement results are shown in Table 1.
In addition, follow-up observation records are shown below.

・ July of July: The water of pond A was collected and microscopically observed (400 times, the same in the following). Flocc (Bacterium bacillus) in which Bacillus subtilis in the pond salon contained Chlamydomonas (green algae) etc. Since superior bacteria were observed, it was found that Bacillus subtilis was a dominant bacterium. In the pond A, the coloration was brown, with the floc sinking to the bottom in a sludge form and algae reduced. The microscope picture of water collected from two places of pond A is shown in Drawing 2 (a) and (b).
The pond B was in a state in which astronella (diatom) slightly floated. Although the pH value increased as algae grew, it showed a good pH value.

-Mid-May of the following year: Since algae start to breed seasonally, after observing the collected water, 200 g of 200 g bags of pond salon were added to pond A and 2 bags to pond B respectively.

Late August: Both the ponds A and B maintain the transparency of 60 cm or more, the color of the water surface is transparent light brown, and algae are hardly observed. In general, summer is a season when algae strengthens its power, and in the case of a pond where nothing is made, its transparency is about 15 cm.

-Mid October: The pH of both ponds was good. In particular, the water of the pond A was slightly light brown in color, but the transparency was very good at 100 cm or more. When water was collected from two places of pond A and microscopically observed, flocs were observed and algae were hardly observed. The micrographs are shown in FIGS. 3 (a) and 3 (b).

(Example 2)
In mid-September, the pond C of the golf course B (1800 to 2000 m ² , water depth about 25 cm) was observed.
Pond C had a pale green color due to algae, and the transparency was 13 cm.
When water was collected from two places of the pond C and microscopically observed, Chlamydomonas, Anchovy (green algae), Microcystis (cyanobacteria), Actinostorum, Synedola (diatoms) etc. were observed. The micrographs are shown in FIGS. 4 (a) and 4 (b).
The pH value, COD value and BOD value of the collected water were measured. The measurement results are shown in Table 2 below.
Next, in the pond C, 10 bags (total 2000 g) of the "pound salon" containing 200 g were loaded.
Thereafter, observation was continued at the timing shown in Table 2 below, and the fluoroscopy degree, the pH value of the collected water, the COD value, and the BOD value were measured. The measurement results are shown in Table 2.
Also, the follow-up records are shown below.

-Mid-October: Swimming of salmon was confirmed, and sludge was observed at the bottom.
As a result of collecting water from two places of the pond C and observing it with a microscope, it turned out that algae, such as Microcystis, are reducing. The micrographs are shown in FIGS. 5 (a) and 5 (b).

-Mid-November: Swimming of salmon was confirmed, and sludge was observed at the bottom. The COD value and BOD value of the water of the pond C were both very good with groundwater level. However, since the water depth of the pond C is shallow, there is a possibility that the sludge is rolled up by the wind and the visibility is deteriorated. As a result of collecting water from two places of the pond C and observing it under a microscope, algae such as microkistis decreased, and flocc of Bacillus subtilis incorporating algae was observed. The micrographs are shown in FIGS. 6 (a) and 6 (b).

Mid-December: Bottom sludge was clearly observed. As a result of collecting water of the pond C and observing it with a microscope, as in the previous time, algae such as Microkistis were few, and many flocs were observed.

・ The following year, mid-January: The bottom of the sludge can be observed more clearly than the previous time, and it has been found that the transparency is rising. As a result of collecting water from two places of the pond C and observing it with a microscope, algae such as microcystis were few and good floc was observed. The micrographs are shown in FIGS. 7 (a) and 7 (b).

Mid-February: Due to the influence of rain for 2 to 3 days ago, the fluoroscopy degree decreased but the water quality was good. Moreover, as a result of collecting the water of pond C and observing under a microscope, it was confirmed that there is almost no growth of algae. Soon, the water temperature will rise and it will be the season when algae reproduction becomes active. As a precaution, two bags (total 400 g) of 200 g bags containing "Pound Salon" were additionally introduced.

-Mid-March: The inflow of rainwater slightly increased the BOD value, and because the wind was strong, the sludge of the shallow pond rolled up and the fluoroscopy degree was slightly deteriorated, but the pH value was good. As a result of collecting water of the pond C and observing it under a microscope, almost no algae was observed.

-Mid April: The wind in the shallow pond is strong and windy, and the visibility is slightly deteriorated, but the pH value is good. As a result of collecting water of the pond C and observing it under a microscope, almost no algae was observed.
The measurement results of the transparency, the pH value of the collected water, the COD value, and the BOD value are shown in Table 2 below.

(Example 3)
In pond D (500 m ² , water depth 100 cm) of golf course C, two bags (total 800 g) containing 400 g of “Pound Salon” were placed in March. After that, in May and July, the same amount of "pound salon" was introduced.

An overview photograph of pond D taken in July and August is shown in FIGS. Also, an overview photograph of the pond D taken in July of the previous year is shown in FIG.
As can be seen from the photograph of FIG. 10, pond D frequently suffers from a large number of amidoro and the like in July each year. On the other hand, as can be seen from the photographs in Figs. 8 and 9, when "Pound Salon" was added, algae generation was also observed in July and August, which is the time when conventional algae are abundantly generated. In addition, the landscape was good and there was no need to clean it (in FIG. 8, it is water plants to be seen in front).

(Example 4)
In the middle of October, when the pool season is over, we observed the outdoor pool A (350 m ³ , water depth 1 to 1.4 m) of the school A which was kept covered with water.
The pool water had a pale green color due to algae, and the perspective was 24 cm.
Water was collected from two places of the pool A and observed with a microscope. Chlamydomonas, Antrodia camphorata (green algae), blue flour oyster, Microcystis (cyanobacteria), Actinostorum, sinedola (diatoma) etc. were observed. The microscope observation photograph is shown to FIG. 11 (a), (b).
Moreover, pH value, COD value, and BOD value of the collected water were measured. The measurement results are shown in Table 3 below.

Next, four bags (total 800 g) were loaded into the pool A, each containing 200 g of the “pound salon”.
Thereafter, the pool was observed every one to two months, and measurement of the fluoroscopy degree, measurement of pH value of collected water, COD value, BOD value, and microscopic observation were performed. The measurement results of the transparency, pH value, COD value and BOD value are shown in Table 3 below.
In addition, follow-up observation records are shown below.

Mid-November: Water collected from pool A was observed microscopically, and Chlamydomonas (green algae) and Microcystis (blue algae) were observed. However, it was found that Bacillus subtilis is becoming a dominant bacterium because flocks formed by bringing Bacillus subtilis in the pond salon with algae were also observed. As a precaution, two bags (400 g) of "pound salon" containing 200 g bags were additionally introduced.

• Mid-December: The pool level was high and full, as it rained a lot. As a result of microscopic observation of the collected water, flocs containing algae such as Chlamydomonas, Kunshomo and Microkistis were observed. Water was collected from two places in pool A and observed with a microscope. The micrographs are shown in FIGS. 12 (a) and (b).

-Late January: The sludge deposited at the bottom was about 1/5 of the conventional sludge. The wall surface was slightly attached to the wall surface of the algae, with only slight feel, and was in a state of being easily peeled off by light rubbing. The sludge component was a mixture of Bacillus subtilis, soil carried by wind, algae, and the like.

・ Early March: The heavy snow of the other day and the heavy rain of the day brought rainwater into the pool and the pool was full, which affected the COD values etc. The algae is thinly attached to the wall, but the algae can be easily peeled off. Two bags (total 400 g) of 200 g bags containing "Pound Salon" were additionally introduced.

-Mid April: There were many rainy days, so Pool A was full. Sludge water was collected from two places at the bottom of the pool A and observed with a microscope. As a result, it was found that little sludge is contained in the bottom sludge, and Bacillus subtilis is dominant. The micrographs are shown in FIGS. 13 (a) and (b). In FIG. 13 (b), colpidium is a kind of protozoa.
Two bags (total 400 g) of 200 g bags containing "Pound Salon" were additionally introduced.

-Mid-May: Although adhesion of algae is observed on the wall surface, it can be easily peeled off with a brush. Sludge water was collected from two places at the bottom of the pool A and observed with a microscope. As a result, it was found that little sludge was contained in the bottom sludge. The micrographs are shown in FIGS. 14 (a) and (b). In FIG. 14 (a), Bortisella is a kind of ciliates.
Two bags (total 400 g) of 200 g bags containing "Pound Salon" were additionally introduced.

-June: The water in the pool was drained, and the wall surface of the pool was observed.
No algae adhesions or stains were observed on any wall or bottom of the pool.
The next day, the students cleaned the pool before it opened. There was a report that cleaning was done simply by lightly rubbing with a polyvinyl alcohol deck brush and letting the water flow, and it was very easy to do compared to the previous year.

(Example 5)
In the same manner as in Example 4, in mid-October, the outdoor pool B (350 m ³ , water depth 1 to 1.4 m) of the school B, which was kept covered with water, was observed.
The water in pool B had a pale green color due to algae, and the perspective was 32 cm.
As a result of microscopic observation of water collected from the pool B, Chlamydomonas (green algae), Microcystis (blue algae), Synedola (diatoms) and the like were observed.
The pH value, COD value and BOD value of the collected water were measured. The measurement results are shown in Table 3 below.

  Next, 2 bags (total 400 g) containing 200 g bags of “Pound Salon” (made by Towa Enzyme Co., Ltd.) (400 g in total) were put into the pool B in the same manner as in Example 4. , The measurement of the pH of the collected water, the COD value, the BOD value, and the microscopic observation. The measurement results of the transparency, pH value, COD value and BOD value are shown in Table 3 below.

The appearance of the water put in the pool B, as a result of microscopic observation, the condition of the pool cleaning before opening the pool was almost the same as the pool A of Example 4.
In addition, about Drawing 1-Drawing 14, a color drawing is submitted by a separate article submission form.

1 · · · algae 2 · · · flock formed by Bacillus subtilis

Claims (7)

It is a microbial preparation mainly composed of dried powder obtained by fermenting natural plants with Bacillus subtilis in a closed water system, the number of cells is 1 × 10 ⁹ cells / g or more, and the average particle size is 0.8 mm or less how certain ones at a rate of 100~10,000g per water 300 meters ^3, characterized in that it is turned on, to suppress the propagation of algae in a closed water system, to purify water. The method for suppressing algae reproduction in a closed water system and purifying water quality according to claim 1, wherein the microbial preparation is injected at least once between March and June or September and December. The method for suppressing the growth of algae in the closed water system according to claim 1 or 2, and purifying the water quality, characterized in that the microbial preparation is enclosed in a bag of a water-soluble film and introduced. The maximum water depth of the said closed water system is 2 m or less, The reproduction of the algae in the closed water system in any one of the Claims 1-3 in any one of the Claims 1-3, The method of purifying water quality . The said closed water system is a golf course pond, a garden pond, a dam, a reservoir, a reservoir, a pool, or a swamp, The reproduction of the algae in the closed water system according to any one of claims 1 to 4 is suppressed And how to purify the water quality . The closure according to any one of claims 1 to 5, characterized in that said closed water system is a golf course pond and said microbial preparation is introduced at least once between September and November or March and May. A method to control the reproduction of algae in the water system and purify the water quality . The closed water system is an off-season outdoor pool, and the microbial preparation is introduced at least once between September and November, and is further injected every one to two months until next season's pool cleaning. The method of suppressing the reproduction of the algae in the closed water system according to any one of claims 1 to 5 and purifying the water quality .

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