JP6456530B1 - Blood coagulation time measurement cartridge and blood coagulation time measurement device - Google Patents

Abstract

A cartridge for measuring blood coagulation time, which has a relatively simple structure and can be reduced in cost, and a blood coagulation time measuring apparatus using the same.
A blood coagulation time measurement cartridge according to the present invention is provided on one end side of a measurement channel 3 and is provided with an inlet 4 through which blood can be introduced, and on the other end side of the measurement channel 3. A communication port 7 capable of sucking or pressurizing blood introduced into the measurement channel 3 from the air 3 or the inlet 4, a moving body 16 arranged in the measurement channel 3 and movable in the measurement channel 3, The coagulation promoter applied to at least one of the flow channel wall surface and the moving body 16 that defines the measurement flow channel 3 and a predetermined portion of the measurement flow channel 3 can transmit light, and air can be passed through the communication port 7. Alternatively, it includes a moving body 16 that reciprocates in the measurement flow path 3 as blood is aspirated or pressurized, or detection areas 21 and 22 that can detect whether or not blood is present in a predetermined portion by light.
[Selection] Figure 3

Description

  The present invention relates to a blood clotting time measuring cartridge used for measuring the time until blood clots, and a blood clotting time measuring apparatus using the blood clotting time measuring cartridge.

  When extracorporeal circulation such as cardiopulmonary bypass is performed by dialysis or circulatory system surgery, or when performing cardiac catheter treatment, an anticoagulant such as heparin is used to prevent blood coagulation. Yes. These anticoagulants may block the extracorporeal circuit if the dosage is insufficient, and it is difficult to stop hemostasis if the dosage is excessive, so it is important to use an appropriate amount. is there. Conventionally, in such a case, blood coagulation is promoted by, for example, mixing a blood coagulation promoter with blood, and the time (blood coagulation time) at which it is determined that the blood has coagulated at a predetermined ratio is measured. Has adopted a technique for determining the dose and timing of administration of a coagulation promoter, and various techniques have been proposed for that purpose.

  For example, Patent Document 1 includes a measurement cartridge including a bifurcated capillary tube including a constricted portion and a measurement device including an air pump. By setting the cartridge in the measurement device, the air pump is connected to each capillary tube, A technique has been shown in which blood injected into a capillary tube is reciprocated with an air pump to promote coagulation in a constricted portion, and blood coagulation time is measured from a change in time required for this reciprocation.

  Patent Document 2 includes a measurement cartridge in which a sphere is arranged in a measurement channel and a measurement device capable of swinging the set measurement cartridge, and the sphere is measured together with blood as the cartridge swings. The blood coagulation time is determined from the change in the time required for the reciprocating movement of the blood measured at that time by dissolving the anticoagulant previously applied to the measurement channel and thereby promoting the coagulation. Techniques for measuring are shown.

US Pat. No. 5,372,946 JP2015-200612A

  By the way, the flow path through which blood moves in such a measurement cartridge is required to have high dimensional accuracy because variations in shape and size affect the coagulation time, and this leads to an increase in cost. It is. Here, the measuring cartridge of Patent Document 1 has a long length because the capillaries are divided into two branches as described above, so that the cost increases accordingly. In addition, when connecting an air pump to a capillary tube, the pressure of the air pump must be transmitted to the capillary tube without leakage for performance, and for that purpose, the air pump and the capillary tube must be connected in an airtight state. Both the apparatus and the measurement cartridge require high dimensional accuracy at the connecting portion. This has spurred an increase in cost. In addition, since the air pump must employ a micropump that can accurately control a small amount of air, the cost is further increased.

  On the other hand, the measurement cartridge of Patent Document 2 can be formed relatively easily because only one measurement channel is provided in a straight line. However, since it is necessary to provide a swinging mechanism in the measuring apparatus, there is still room for improvement in terms of cost reduction as a whole.

  An object of the present invention is to solve such problems, and uses a blood coagulation time measuring cartridge that has a relatively simple structure and can reduce costs, and uses the blood coagulation time measuring cartridge. An object of the present invention is to provide a blood coagulation time measurement device.

  The present invention provides a measurement channel extending long with respect to the cross-sectional area, an inlet provided on one end side of the measurement channel, and capable of introducing blood, and provided on the other end side of the measurement channel. A communication port capable of aspirating or pressurizing blood introduced into the measurement channel from the air or the inlet of the measurement channel, and a movable body arranged in the measurement channel and movable in the measurement channel , A coagulation promoter applied to at least one of the channel wall surface defining the measurement channel and the movable body, and light can be transmitted to a predetermined portion of the measurement channel, from the communication port A blood coagulation time measurement comprising: a detection area capable of detecting by light whether or not the moving body or blood that reciprocates in the measurement flow path as air or blood is sucked or pressurized is present in the predetermined portion. An air chamber communicating with the communication port; and A diaphragm that closes the chamber and pressurizes or depressurizes the air chamber to reciprocate the moving body and blood, the diaphragm having a cylindrical shape and having an inner space as the air chamber; The cartridge for blood coagulation time measurement is formed between an annular ring holder and a holder inserted through the cylindrical wall on the outer side in the radial direction of the cylindrical wall.

  In such a blood coagulation time measurement cartridge, it is preferable that the measurement flow path has a narrowed portion in the vicinity of the detection area.

  Moreover, it is preferable to provide the shielding part which prevents that a blood flows toward the said detection area in the outer side of the said inlet.

  The present invention also provides a measurement channel extending long with respect to the cross-sectional area, an inlet through which one end of the measurement channel is introduced, blood can be introduced, and another end of the measurement channel. A communication port that can suck or pressurize blood introduced into the measurement channel from the measurement channel air or the injection port, and a reservoir that is connected to the communication port and stores blood flowing out from the communication port At least one of a chamber, a stirrer arranged in the storage chamber and stirring the blood in the storage chamber, a channel wall surface defining the measurement channel, a storage chamber wall surface defining the storage chamber, and the agitator Light can be transmitted to a predetermined portion of the measurement flow channel and the coagulation accelerator applied to the measurement channel, and reciprocate in the measurement flow channel as air or blood is sucked or pressurized from the communication port. Detection capable of detecting by light whether blood is present in the predetermined part The rear is also a blood coagulation time measurement cartridge comprising a.

  Here, it is preferable that the measurement flow path includes a narrowed portion in the vicinity of the detection area.

  Moreover, it is preferable to provide the shielding part which prevents that a blood flows toward the said detection area in the outer side of the said inlet.

  Furthermore, it is preferable to include an air chamber that communicates with the storage chamber, and a diaphragm that closes the air chamber and pressurizes or depressurizes the air chamber to reciprocate blood in the measurement channel.

  In the blood coagulation time measuring device in which the above-described blood coagulation time measuring cartridge is set, a detection means provided at a position corresponding to the detection area and capable of detecting the moving body or blood by light, and the diaphragm It is preferable to include pressing means that can be pushed in or pulled up by a predetermined amount of movement.

  In the blood coagulation time measurement cartridge of the present invention, the measurement flow path through which the blood moves is a single flow path that extends long with respect to the cross-sectional area, and therefore can be formed relatively easily. Moreover, since the blood coagulation time is measured by sucking or pressurizing air or blood in the measurement flow path without using the swing mechanism as in Patent Document 2, the cost can be reduced.

  In the case where the moving body is arranged in the measurement channel, the blood can be stirred by the reciprocating motion of the moving body in the measurement channel, so that at least one of the channel wall surface defining the measurement channel and the moving body. The coagulation promoter applied to one can be dissolved efficiently and stably in blood. That is, since the variation when the coagulation accelerator dissolves in the blood is suppressed, the error factor of the coagulation time can be removed and the coagulation time can be stably measured. In addition, since a minute gap through which blood can pass is formed between the outer peripheral surface of the moving body and the inner peripheral surface of the measurement flow path, the movement of the moving body slows down when the blood begins to coagulate in this gap. Thus, the cycle of the reciprocating motion of the moving body becomes longer with respect to the state where the blood is not coagulated. For this reason, the blood coagulation time can be derived from the difference between the cycles. When a moving body is provided, it is possible to detect whether or not a moving body that reciprocates exists in the predetermined portion by providing a detection area capable of transmitting light to a predetermined portion of the measurement channel. Is possible. In the detection area, blood reciprocating with the moving body may be detected.

  In addition, in the case where the stirrer is arranged in the storage chamber, the coagulation accelerator applied to at least one of the flow channel wall surface defining the measurement flow channel, the storage chamber wall surface defining the storage chamber, and the stirrer is efficiently used. Can be dissolved in blood. That is, also in this case, since the variation when the coagulation accelerator is dissolved in the blood is suppressed, the error factor of the coagulation time can be removed and the coagulation time can be stably measured.

  If the stenosis part is provided in the vicinity of the detection area in the measurement channel, the clotted blood or the mobile body to which the coagulated blood adheres is easily caught by the stenosis part. Can be detected.

  In the case where the moving body is arranged, the air chamber is connected to the communication port, and the diaphragm is configured to close the air chamber and pressurize or depressurize the air chamber to reciprocate the moving body. The one provided with the stirrer is configured to include an air chamber that communicates with the storage chamber, and a diaphragm that closes the air chamber and pressurizes or depressurizes the air chamber to reciprocate the blood in the measurement channel. This eliminates the need for a connection portion that must connect the air pump and the capillary tube in a hermetic state as in Patent Document 1, thereby reducing costs. Further, in the blood coagulation time measurement apparatus equipped with the blood coagulation time measurement cartridge according to the present invention, the pressure of the air chamber can be adjusted by changing the amount of pushing into the diaphragm. There is no need to use an expensive micropump, and cost can be reduced in this respect as well.

  In addition, in the case where a shielding part that prevents blood from flowing toward the detection area is provided outside the injection port, even if blood is spilled when blood is injected into the injection port, the shielding part causes the blood to enter the detection area. Therefore, there is no problem that the measurement of the coagulation time is hindered or the cartridge cannot be used due to spilled blood.

1 is a perspective view showing a first embodiment of a blood clotting time measuring cartridge according to the present invention. FIG. (A) is a plan view, (b) is a front view, and (c) is a right side view of the cartridge for blood coagulation time measurement of the first embodiment. (A) is sectional drawing which follows AA shown to Fig.2 (a) about the cartridge for blood coagulation time measurement of 1st Embodiment, (b) is a bottom view. It is the figure which showed the modification of 1st Embodiment. FIG. 2A is a cross-sectional view corresponding to AA shown in FIG. 2A, and FIG. 2B is a bottom view showing a second embodiment of a blood clotting time measuring cartridge according to the present invention. It is the figure which showed the modification of 2nd Embodiment.

  A first embodiment of a blood coagulation time measuring cartridge (hereinafter referred to as “cartridge”) according to the present invention will be described below with reference to FIGS.

  The cartridge according to the present embodiment includes a base 1 that is flat as a whole and a thin plate-like closing plate 2 that is fixed to the base 1 on the bottom surface side of the base 1.

  The base 1 is made of a colorless and transparent synthetic resin. In addition, it is not restricted to a synthetic resin, You may form with glass etc. Moreover, it is not limited to colorless and transparent, and may be colored and transparent that allows light to pass through at least a detection area described later. Further, the area other than the detection area may not be transparent. The blocking plate 2 is formed of a colorless and transparent synthetic resin (thin sheet). However, as with the base 1, it can be formed of various materials and colors as long as the function of the present invention is satisfied. The base 1 and the closing plate 2 may be bonded with an adhesive, or may be welded using, for example, ultrasonic waves.

  As shown in FIG. 3, a groove extending long with respect to the cross-sectional area is provided on the bottom surface of the base 1, and a measurement channel 3 is formed between the base plate 1 and the closing plate 2. One measurement flow path 3 of the present embodiment is provided at the center in the width direction of the base 1.

  An inlet 4 through which blood can be introduced is provided at one end side of the measurement channel 3. The inlet 4 is connected to an opening 5 having an outer peripheral surface having a cylindrical shape and an inner peripheral surface having a conical shape. Further, an excess blood receiving portion 6 having an annular shape is provided on the outer side in the radial direction of the opening 5.

  A communication port 7 is provided on the other end side of the measurement channel 3. The communication port 7 can suck or pressurize the air in the measurement channel 3 or the blood introduced into the measurement channel 3 from the injection port 4 by changing the amount of pushing into the diaphragm described later.

  The central portion of the measurement channel 3 is provided with one end-side convex portion 8 and the other end-side convex portion 9 that are provided so as to protrude toward the bottom surface side. As shown in the figure, a narrowed portion (one narrowed portion 10 and one narrowed portion 11 on the other end side) is formed by narrowing the measurement channel 3.

  The base 1 includes a cylindrical wall 12 that is entirely cylindrical on the opposite side of the opening 5 and the excess blood receiving portion 6 described above. The inner peripheral surface of the cylindrical wall 12 has a shape in which the bottom surface side has a small diameter and the top surface side has a large diameter across a diameter-enlarging portion. The inner space of the cylindrical wall 12 is connected to the communication port 7 on the bottom surface side. In the present embodiment, the entire inner space of the cylindrical wall 12 is referred to as an air chamber 13.

  A diaphragm 14 for closing the air chamber 13 is provided on the top surface side of the cylindrical wall 12. The diaphragm 14 is made of, for example, rubber having a small thickness and elasticity. In addition, an annular holder 15 that is inserted and held in the cylindrical wall 12 through the cylindrical wall 12 and that sandwiches the diaphragm 14 between the cylindrical wall 12 is provided outside the cylindrical wall 12 in the radial direction. Is provided. Here, when the diaphragm 14 is pushed toward the bottom surface side, the volume of the air chamber 13 is reduced, so that the air chamber 13 can be pressurized. Further, if the diaphragm 14 is pushed toward the bottom surface in the initial state, the volume of the air chamber 13 is increased by loosening the push, so that the air chamber 13 can be decompressed. The air chamber 13 may be depressurized by pulling up the diaphragm 14 toward the top surface.

  In the measurement channel 3, a moving body 16 is disposed between the one end side narrowed portion 10 and the other end side narrowed portion 11. The moving body 16 has an outer diameter that is slightly smaller than the inner diameter of the measurement channel 3 and can move within the measurement channel 3. A minute gap through which blood can pass is formed between the outer peripheral surface of the moving body 16 and the inner peripheral surface of the measurement flow path 3. The outer diameter of the moving body 16 is formed so as to be larger than the gap between the one end side narrowed portion 10 and the other end side narrowed portion 11. Here, the moving body 16 of the present embodiment has a cylindrical shape and is made of aluminum. In addition, the shape of the moving body 16 may be a prism, or may be an ellipse whose end portion has a smaller thickness than the central portion in the longitudinal direction. It may be spherical. Various materials such as other metals and synthetic resins can be applied to the moving body 16.

  By the way, a flow channel wall surface defining the measurement flow channel 3 (in this embodiment, an inner surface of a groove provided at the center in the width direction of the base 1) is coated with a coagulation promoter that promotes blood coagulation. The coagulation accelerator may be applied to the entire top surface of the blocking plate 2 facing the measurement channel 3 and applied to the entire measurement channel 3 or applied to the moving body 16. Also good.

  The top surface of the base 1 is provided with an outer edge wall 17 which is located at the outer edge of the base 1 and surrounds the holder 15 and has a U-shape in plan view. As shown in FIG. 1, a plate-like portion (shielding portion) 19 that connects the left and right outer edge walls 17 is provided between the excess blood receiving portion 6 and the holder 15. Further, the vicinity of the excess blood receiving portion 6 on the longitudinal side surface of the base 1 has an uneven shape, and a cartridge is set or removed from a blood coagulation time measuring device (hereinafter referred to as “measuring device”). A finger hooking portion 20 is provided as a part where a finger is hooked when performing the above operation.

  Further, the base 1 and the blocking plate 2 are provided with a detection area capable of transmitting light to a predetermined portion of the measurement channel 3. In the present embodiment, as shown in FIG. 3A, the one end side detection area 21 located near the one end side constriction portion 10 and on the other end side constriction portion 11 side, and the other end side constriction portion 11 The other end side detection area 22 located in the vicinity and the one end side constriction part 10 side is provided. The one end side detection area 21 is located closer to the other end side detection area 22 with respect to the shielding part 19.

  Such a cartridge according to the first embodiment can be set in a measuring device (not shown) to measure blood clotting time. Specifically, the side of the cartridge 14 where the diaphragm 14 is located is inserted into the measuring device, and the cartridge is set in the measuring device. In this state, the opening 5 is located outside the measuring device. Further, as shown in FIG. 3A, the measuring apparatus includes a pressing means S1 that can push the diaphragm 14 by a predetermined moving amount, and a detecting means that can detect the presence or absence of the moving body 16 by light. Yes. The detection means in the present embodiment is composed of one end side light source S2 and one end side light receiving sensor S3 provided so as to sandwich the measurement flow path 3 in the one end side detection area 21, and in the other end side detection area 22, The other end side light source S4 and the other end side light receiving sensor S5 are configured, and the moving body 16 blocks the light of the one end side light source S2 and the other end side light source S4, so that the moving body 16 has the one end side detection area 21 and the like. Whether or not it exists in the other end side detection area 22 can be detected. The one end side light source S2 and the other end side light source S4 emit infrared rays. Also, the positions of the one end side light source S2, the one end side light receiving sensor S3, the other end side light source S4, and the other end side light receiving sensor S5 are reversed from the illustrated example, and the one end side light source S2 and the other end side light source S4 are cartridges. The one end side light receiving sensor S3 and the other end side light receiving sensor S5 may be provided on the top surface side of the cartridge. Further, instead of such a transmission type sensor, a reflection type sensor may be used.

  And after setting a cartridge in a measuring apparatus, the blood to measure is inject | poured into the opening part 5 with a dispenser etc. FIG. When injecting blood, the diaphragm 14 is pushed in by the pressing means S1 in advance to reduce the volume of the air chamber 13. Here, it is sufficient that the amount of blood injected does not spill from the opening 5, but even when a large amount of blood is injected, the surplus blood receiving portion 6 is provided outside the opening 5 in the radial direction. Blood spilled from the opening 5 can be stored in the excess blood receiving part 6. Furthermore, since the shielding part 19 is provided outside the surplus blood receiving part 6, even if the blood may overflow beyond the surplus blood receiving part 6, the one end side detection area 21 or the other end side detection area 22 does not flow.

  Thereafter, the air chamber 13 is depressurized by pulling back the pressed pressing means S1, so that the air in the measurement channel 3 is sucked from the communication port 7 and the blood injected into the opening 5 is taken into the measurement channel 3 Can be drawn into. In this state, blood is drawn until the other end side constriction 11 is exceeded. Thereby, the moving body 16 moves to the other end side constriction part 11 side with the blood. Here, whether or not the moving body 16 has moved to the vicinity of the other end side constriction portion 11 can be confirmed by whether or not the light from the other end side light source S4 can be detected by the other end side light receiving sensor S5. .

  After that, the air chamber 13 is pressurized by pushing the pressing means S1 again, and the air and blood in the measurement channel 3 are pressurized accordingly. Move towards. Here, as described above, whether or not the moving body 16 has moved to the vicinity of the one-end-side constriction portion 10 can be detected by the one-end-side light source S2 and the one-end-side light receiving sensor S3.

  Since the air chamber 13 can be pressurized or depressurized by repeating pushing and pulling of the pressing means S1 in this way, air or blood in the measurement channel 3 is pressurized or sucked through the communication port 7, Accordingly, the moving body 16 can be reciprocated with blood. Thereby, the blood in the measurement channel 3 is agitated by the moving body 16, and the coagulation promoter applied to the channel wall surface defining the measurement channel 3 can be dissolved in the blood efficiently and stably. In addition, since the outer diameter of the moving body 16 is formed so as to be larger than the gap between the one end side constricted portion 10 and the other end side constricted portion 11, the one end side constricted portion 10 and the other end side during the reciprocating motion. It does not exceed the constriction 11. In addition, when air is taken into the blood injected into the cartridge, air bubbles may be generated in the measurement flow path 3. In the conventional cartridge, this air bubble may be erroneously detected as coagulated blood. However, in the present embodiment, the threshold value of the one-end side light receiving sensor S3 or the like is optimized to detect the moving body 16, so that the influence of air bubbles on blood coagulation time measurement can be suppressed. it can.

  Then, when the coagulation promoter is dissolved by the stirring of blood by the moving body 16, the blood gradually coagulates, so that the moving body 16 gradually moves slowly. That is, even if the timing of pushing and pulling back of the pressing means S1 does not change, the cycle of the reciprocating motion of the moving body 16 detected by the one end side light receiving sensor S3 and the other end side light receiving sensor S5 when the blood is not coagulated Since the period of the reciprocating motion detected by the one end side light receiving sensor S3 or the like changes when the movement of the moving body 16 becomes slow, the blood coagulation time is determined based on the change in the time required for the reciprocating motion. Can be calculated. As described above, a minute gap through which blood can pass is formed between the outer peripheral surface of the moving body 16 and the inner peripheral surface of the measurement flow path 3, and when the blood begins to coagulate in this gap, the moving body. 16 moves slowly. That is, since the cycle in which the moving body 16 reciprocates becomes longer than before the blood coagulates, the blood coagulation time can be derived from the difference in the cycle. Since the gap is small, the movement of the moving body 16 is affected as soon as the blood starts to coagulate. For this reason, blood coagulation time can be measured in a short time.

  In the present embodiment, the moving body 16 that reciprocates is detected by the one-end light receiving sensor S3 or the like. However, the target to be detected by the one-end light receiving sensor S3 or the like may be blood. In this case, as the clotting progresses, the blood clot becomes larger and closes the one end side constriction 10 or the other end constriction 11, which obstructs the reciprocating blood flow, The blood detected by the one end side light receiving sensor S3 and the other end side light source S4 is in a state where it cannot be detected. Therefore, the blood coagulation time can be measured based on the time when blood cannot be detected by the one end side light receiving sensor S3 or the other end side light source S4. When blood is detected by the one end side light receiving sensor S3 or the like, as shown in FIG. 4, the one end side detection area 21 is in the vicinity of the one end side constricted portion 10 and opposite to the other end side constricted portion 11. The other end side detection area 22 may be positioned in the vicinity of the other end side constricted portion 11 and on the opposite side to the one end side constricted portion 10.

  Next, a second embodiment of the cartridge according to the present invention will be described. The cartridge of this embodiment is provided with a stirrer 23 instead of the moving body 16 as shown in FIG. 5 with respect to the cartridge of the first embodiment described above. In the present embodiment, the inner space of the cylindrical wall 12 is a space on the bottom surface side that communicates with the communication port 7 (a small-diameter space generally positioned on the bottom surface side in the inner space of the cylindrical wall 12) and the storage chamber 13a. The space on the top surface side that is closed by the diaphragm 14 (in the inner space of the cylindrical wall 12, the space that expands in general and the large-diameter space connected thereto) is referred to as an air chamber 13 b. Since the other parts are basically the same as those of the cartridge of the first embodiment, the same reference numerals are given in the drawings and description thereof is omitted.

  The stirring bar 23 is arranged in the storage chamber 13a. The stirrer 23 is formed of, for example, a magnet or a ferromagnetic material, and can be rotated in the storage chamber 13a by a magnetic field generator S6 provided in the measuring device. In addition, the stirrer 23 of the present embodiment has a cylindrical shape, but may be a prismatic shape or an elliptical shape in which the thickness of the end portion is smaller than the central portion in the longitudinal direction. Moreover, what provided the some blade | wing in the part used as disk shape may be used.

  In addition, a coagulation accelerator that promotes blood coagulation is provided on the wall surface of the storage chamber that defines the storage chamber 13a (in this embodiment, a small-diameter inner peripheral surface located on the bottom surface side of the inner peripheral surface of the cylindrical wall 12). It has been applied. The coagulation promoter may be applied to the channel wall surface that defines the measurement channel 3 or may be applied to the stirrer 23 as in the cartridge of the first embodiment.

  Also in the cartridge of the second embodiment, it is possible to measure the blood coagulation time by setting it in a measuring device (not shown).

  Specifically, like the cartridge of the first embodiment, the diaphragm 14 is pushed in with the pressing means S1 in advance, and then blood is injected into the opening 5 with a dispenser or the like.

  Then, by pulling back the pressed pressing means S1, the air chamber 13b is depressurized. Therefore, the air in the measurement channel 3 is sucked from the communication port 7 through the storage chamber 13a, and the blood injected into the opening 5 is drawn. It can be drawn into the measurement channel 3. Here, in the present embodiment, blood is drawn into the storage chamber 13a beyond the narrowed portion 11 on the other end side. In addition, since there is a correlation between the amount of blood withdrawn by the pressing means S1 and the amount of blood to be drawn, whether or not blood has been drawn up to the storage chamber 13a can be determined by the amount of withdrawing of the pressing means S1. . By the way, in the one end side light receiving sensor S3 and the other end side light receiving sensor S5 in this embodiment, the threshold is optimized so as to detect the presence or absence of blood. For this reason, whether the blood has moved to the vicinity of the one end side constriction part 10 can be detected by whether the light from the one end side light source S2 can be detected by the one end side light receiving sensor S3. Further, whether or not the blood has moved to the vicinity of the other end side constricted portion 11 can be detected by whether or not the light from the other end side light source S4 can be detected by the other end side light receiving sensor S5. For this reason, the time when the blood is detected by the one end side light receiving sensor S3 or the other end side light receiving sensor S5 is used as a reference for the pullback amount of the pressing means S1, and the blood is drawn up to the storage chamber 13a based on the pullback amount therefrom. Also good.

  After the blood is drawn up to the storage chamber 13a, the stirrer 23 is rotated by driving the magnetic field generator S6. Thereby, the coagulation promoter applied to the wall surface of the storage chamber that defines the storage chamber 13a can be efficiently and stably dissolved in blood.

  Then, the air chamber 13b is pressurized by pushing the pressing means S1 again while the stirring bar 23 is rotated (the rotation may be stopped). Thereby, the blood in the storage chamber 13a and the measurement flow path 3 is also pressurized, so that the blood can flow toward the one-side constriction 10. In this way, the blood can be reciprocated in the measurement flow path 3 by repeatedly pushing and pulling the pressing means S1.

  When the coagulation promoter is dissolved by stirring the blood with the stirrer 23, the blood gradually coagulates, whereby the blood clot becomes larger and closes the one end side constricted portion 10 and the other end side constricted portion 11. If it does so, the flow of the blood which was reciprocating will be prevented, and the blood which has been detected by the one end side light receiving sensor S3 and the other end side light source S4 so far becomes undetectable. Therefore, the blood coagulation time can be measured based on the time when blood cannot be detected by the one end side light receiving sensor S3 or the other end side light source S4.

  The cartridge according to the present invention and the measuring apparatus have been described above with reference to specific embodiments. However, the cartridge according to the present invention is not limited to the above-described embodiments, and various modifications can be made within the scope of the claims. The thing which added is included. For example, the storage chamber 13a and the measurement channel 3 shown in FIG. 5 are at the same position on the bottom surface side, but as shown in FIG. 6, the storage chamber 13a is positioned on the bottom surface side with respect to the measurement channel 3. A step may be provided. Since the stirrer 23 is attracted by the magnetic field generator S6, the stirrer 23 does not move to the outside of the storage chamber 13a (measurement flow path 3) even in the cartridge shown in FIG. 5, but like the cartridge shown in FIG. If the step is provided, the stirrer 23 can be more reliably retained in the storage chamber 13a. Various methods for fixing the diaphragm 14 may be employed. For example, an adhesive may be used to fix the diaphragm 14 to the base 1. Further, the detection area may be provided only on one of the one end side and the other end side.

1: Base 2: Blocking plate 3: Measurement flow path 4: Injection port 5: Opening portion 6: Surplus blood receiving portion 7: Communication port 8: Convex portion on one end 9: Convex portion on the other end 10: Constriction portion on one end 11 : Other end side constriction part 12: cylindrical wall 13: air chamber 13a: storage chamber 13b: air chamber 14: diaphragm 15: holder 16: moving body 17: outer edge wall 19: shielding part 20: finger hook part 21: one end side detection Area 22: Other end detection area 23: Stir bar

Claims (9)

A measurement channel extending long with respect to the cross-sectional area;
An inlet provided on one end side of the measurement channel and capable of introducing blood;
A communication port provided on the other end side of the measurement channel, and capable of sucking or pressurizing blood introduced into the measurement channel from the air or the injection port of the measurement channel;
A movable body arranged in the measurement channel and movable along the measurement channel;
A coagulation accelerator applied to at least one of the channel wall surface defining the measurement channel and the moving body;
The movable body or blood that can transmit light to a predetermined portion of the measurement flow path and reciprocates in the measurement flow path as air or blood is sucked or pressurized from the communication port is the predetermined portion. A blood coagulation time measurement cartridge comprising: a detection area capable of detecting by light whether or not it exists in
An air chamber that communicates with the communication port; and a diaphragm that closes the air chamber and pressurizes or depressurizes the air chamber to reciprocate the moving body and blood. The diaphragm has a cylindrical shape and an inner side. A blood coagulation time measurement cartridge that is sandwiched between a cylindrical wall having the space as the air chamber and a holder that is annular and that is inserted radially outward of the cylindrical wall.   The blood coagulation time measurement cartridge according to claim 1, wherein the measurement flow path includes a narrowed portion in the vicinity of the detection area.   The cartridge for blood coagulation time measurement according to claim 1 or 2, further comprising a shielding part for preventing blood from flowing toward the detection area outside the injection port. A measurement channel extending long with respect to the cross-sectional area;
An inlet provided on one end side of the measurement channel and capable of introducing blood;
A communication port provided on the other end side of the measurement channel, and capable of sucking or pressurizing blood introduced into the measurement channel from the air or the injection port of the measurement channel;
A storage chamber connected to the communication port and storing blood flowing out of the communication port;
A stirrer arranged in the storage chamber and stirring the blood in the storage chamber;
A coagulation accelerator applied to at least one of the flow channel wall surface defining the measurement flow channel, the storage chamber wall surface defining the storage chamber, and the stirrer;
Whether there is blood in the predetermined portion that is capable of transmitting light to a predetermined portion of the measurement channel and reciprocates in the measurement channel as air or blood is sucked or pressurized from the communication port A blood coagulation time measurement cartridge comprising: a detection area capable of detecting whether or not by light.   The blood coagulation time measurement cartridge according to claim 4, wherein the measurement flow path includes a narrowed portion in the vicinity of the detection area.   The blood coagulation time measurement cartridge according to claim 4, further comprising a shielding portion that prevents blood from flowing toward the detection area outside the injection port.   The air chamber communicating with the storage chamber and a diaphragm for closing and closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate blood in the measurement flow path. The cartridge for blood coagulation time measurement according to one item. A blood coagulation time measuring apparatus for setting a blood clotting time measurement cartridge according to claim 1, 2, 3,
A blood coagulation time measuring device provided with a detecting means provided at a position corresponding to the detection area and capable of detecting the moving body or blood by light and a pressing means capable of pushing or pulling up the diaphragm by a predetermined moving amount.   A blood coagulation time measuring device for setting the blood coagulation time measuring cartridge according to claim 7,
  A blood coagulation time measuring apparatus, comprising: a detecting means provided at a position corresponding to the detection area and capable of detecting blood by light; and a pressing means capable of pushing or lifting the diaphragm by a predetermined movement amount.

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