JP2019124548A - Cartridge for measuring blood coagulation time, and blood coagulation time measuring device - Google Patents

Abstract

To provide a cartridge for measuring blood coagulation time, which has a relatively simple structure and can suppress costs, and a blood coagulation time measuring device using the cartridge.SOLUTION: A cartridge for measuring blood coagulation time includes: an injection port 4 which is disposed on one end side of a measurement channel 3 and capable of introducing blood; a communication port 7 which is disposed on the other end side of the measurement channel 3, and capable of suctioning or pressurizing air of the measurement channel 3 or blood introduced from the injection port 4 into the measurement channel 3; a mobile body 16 which is provided in the measurement channel 3 and is movable in the measurement channel 3; a coagulation accelerator coated on at least one of either a channel wall surface defining the measurement channel 3 or the mobile body 16; and detection areas 21 and 22 which can transmit light to a prescribed portion of the measurement channel 3, and which can detect using the light whether the mobile body 16 or blood reciprocating in the measurement channel 3 in accompany with the suctioning or pressurization of air or blood from the communication port 7, is present at the prescribed portion.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a cartridge for measuring blood coagulation time used when measuring time until blood is coagulated, and a blood coagulation time measuring device using the cartridge for measuring blood coagulation time.

  When performing extracorporeal circulation such as cardiopulmonary bypass, which is performed by dialysis or surgery of the circulatory system, or when performing treatment of a cardiac catheter, an anticoagulant such as heparin is used to prevent coagulation of the blood. There is. It is important to use such an anticoagulant in an appropriate amount because it may block the extracorporeal circuit if the dose is insufficient, and hemostasis will be difficult if the dose is excessive. is there. Conventionally, in such a case, for example, the blood coagulation is promoted by mixing a blood coagulation promoter into the blood, and the time during which the blood is judged to be coagulated at a predetermined ratio (blood coagulation time) is measured. Thus, techniques for determining the dose and timing of administration of a coagulation promoter have been incorporated, and various techniques have been proposed for this purpose.

  For example, Patent Document 1 includes a measurement cartridge including a bifurcated capillary tube including a narrowed portion and a measurement device including an air pump, and the air pump is connected to each capillary tube by setting the cartridge in the measurement device; A technique for promoting coagulation in a narrowed portion by reciprocating a blood introduced into a capillary tube with an air pump and measuring a coagulation time of blood from a change in time required for the reciprocating movement is disclosed.

  Further, Patent Document 2 includes a measurement cartridge in which a sphere is disposed in the measurement flow channel, and a measurement device capable of swinging the set measurement cartridge, and the flow channel is used to measure the sphere together with blood as the cartridge swings. It is made to reciprocate within, thereby dissolving the anticoagulant that has been applied in advance to the measurement channel to promote coagulation, and the change of the time required for the reciprocating of the blood measured at that time to the coagulation time of the blood The technology for measuring

U.S. Pat. No. 5,372,946 Unexamined-Japanese-Patent No. 2015-200612 gazette

  By the way, in such a measurement cartridge, the flow path in which the blood moves has an influence on the coagulation time if the shape and size vary, so high dimensional accuracy is required, which causes an increase in cost. It is Here, in the measurement cartridge of Patent Document 1, as described above, since the capillary is bifurcated and the length is long, the cost is increased accordingly. In addition, in order to connect the air pump to the capillary tube, the pressure of the air pump must be transmitted to the capillary tube without leakage for performance, and the air pump and the capillary tube must be airtightly connected for that purpose. Both the apparatus and the measuring cartridge require high dimensional accuracy at the connection. For this reason, cost increases are being spurred. Further, as the air pump, it is necessary to employ a micro pump that can control a small amount of air with high accuracy, which further increases the cost.

  On the other hand, the measurement cartridge of Patent Document 2 can be relatively easily formed since only one measurement flow channel is provided in a straight line. However, since it is necessary to provide a rocking mechanism in the measuring apparatus, there is still room for improvement in cost control as a whole.

  An object of the present invention is to solve such a problem, and a cartridge for measuring blood coagulation time which is relatively simple in structure and can suppress the cost and a cartridge for measuring blood coagulation time are used. An object of the present invention is to provide a blood coagulation time measurement device.

  The present invention is provided with a measurement flow channel which extends long with respect to a cross-sectional area, an injection port which is provided on one end side of the measurement flow channel and can introduce blood, and is provided on the other end side of the measurement flow channel. A communication port capable of sucking or pressurizing air in the measurement flow channel or blood introduced into the measurement flow channel from the injection port, a movable body disposed in the measurement flow channel and movable in the measurement flow channel A coagulation promoting agent applied to at least one of a flow path wall surface defining the measurement flow path and the movable body, and light can be transmitted to a predetermined portion of the measurement flow path, from the communication port A detection area capable of detecting by light whether or not the moving body or blood reciprocated in the measurement channel along with suction or pressurization of air or blood is detected by light Cartridge.

  In such a blood coagulation time measurement cartridge, preferably, the measurement flow path includes a constriction portion in the vicinity of the detection area.

  Moreover, it is preferable to provide the shielding part which blocks | prevents the blood from flowing toward the said detection area in the outer side of the said inlet.

  Furthermore, it is preferable to provide an air chamber communicating with the communication port, and a diaphragm for closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate the moving body and the blood.

  Further, according to the present invention, there is provided a measurement flow channel which extends long with respect to a cross-sectional area, an injection port which is provided on one end side of the measurement flow channel, and which can introduce blood and the other end side of the measurement flow channel And a communication port capable of suctioning or pressurizing air in the measurement channel or blood introduced into the measurement channel from the injection port, and the communication port connected to the communication port and storing blood flowing out from the communication port. Chamber, a stirring bar arranged in the storage chamber for stirring blood in the storage chamber, a channel wall defining the measurement channel, a storage chamber wall defining the reservoir, and at least one of the stirrer Light can be transmitted to a predetermined portion of the measurement channel, and reciprocates in the measurement channel as air or blood is sucked or pressurized from the communication port Light detectable detection of whether blood is present in the predetermined part The rear is also a blood coagulation time measurement cartridge comprising a.

  Here, it is preferable that the measurement flow path includes a constriction portion in the vicinity of the detection area.

  Moreover, it is preferable to provide the shielding part which blocks | prevents the blood from flowing toward the said detection area in the outer side of the said inlet.

  Furthermore, it is preferable to include an air chamber communicating with the storage chamber, and a diaphragm for closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate blood in the measurement flow path.

  Then, in the blood coagulation time measurement apparatus for setting the above-described blood coagulation time measurement cartridge, the detection unit is provided at a position corresponding to the detection area and can detect the moving body or blood by light, and the diaphragm It is preferable to provide a pressing means capable of pushing or pulling up by a predetermined movement amount.

  In the blood coagulation time measurement cartridge of the present invention, the measurement flow path through which the blood moves can be formed relatively easily because it is a single flow path which extends long with respect to the cross-sectional area. In addition, since the coagulation time of blood is measured by suctioning or pressurizing air or blood in the measurement channel regardless of the swing mechanism as in Patent Document 2, cost can be suppressed.

  In the case where the movable body is disposed in the measurement flow channel, blood can be agitated by reciprocating the movable body in the measurement flow channel, so at least one of the flow path wall surface defining the measurement flow channel and the movable body The procoagulant that is applied to one can be dissolved efficiently and stably in the blood. That is, since the dispersion | variation at the time of a coagulation | solidification promoter melt | dissolves in blood is suppressed, the error factor of coagulation | solidification time can be remove | excluded and measurement of coagulation | solidification time can be performed stably. In addition, since a minute gap through which blood can pass is formed between the outer circumferential surface of the moving body and the inner circumferential surface of the measurement channel, movement of the moving body is slowed when blood starts to coagulate in this gap. As a result, the cycle of the reciprocation of the moving body is longer than in the state where the blood is not coagulated. Therefore, the blood coagulation time can be derived from the difference in this cycle. When a movable body is provided, it is possible to detect whether a reciprocating movable body is present at a predetermined portion of the measurement flow path by providing a detection area capable of transmitting light to the predetermined portion. It is possible. In the detection area, blood reciprocating with the moving body may be detected.

  In addition, in the case where a stirrer is disposed in the storage chamber, the coagulation accelerator applied to at least one of the flow passage wall defining the measurement flow passage, the storage chamber wall defining the storage chamber, and the stirrer is efficiently used. It can be dissolved in blood. That is, also in this case, since the variation when the coagulation accelerator dissolves in blood is suppressed, the error factor of the coagulation time is removed and the coagulation time can be stably measured.

  In the case where the narrowing portion is provided in the vicinity of the detection area in the measurement channel, the coagulated blood and the moving body to which the coagulated blood adheres are easily caught in the narrowing portion, so the blood and the moving body are stably stabilized in the detection area. It can be detected.

  In the case where the movable body is disposed, an air chamber communicating with the communication port, a diaphragm for closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate the movable body are provided. In the case where a stirrer is provided, an air chamber communicating with the storage chamber, and a diaphragm closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate blood in the measurement flow path are provided. This eliminates the need for the air pump and the connection portion for connecting the capillary tube in an airtight state as in Patent Document 1, thereby reducing the cost. Further, in the blood coagulation time measurement apparatus equipped with the blood coagulation time measurement cartridge according to the present invention, since the pressure of the air chamber can be adjusted by changing the amount of pressing on the diaphragm, such as disclosed in Patent Document 1 It is not necessary to use an expensive micro pump, and this can also reduce costs.

  In addition, when a shield is provided outside the injection port to prevent blood from flowing toward the detection area, the blood flow to the detection area by the shield even if blood spills into the injection port. Can not flow, so there will not be problems such as the measurement of the coagulation time being disturbed or the spilled blood making the cartridge unusable.

FIG. 1 is a perspective view showing a first embodiment of a blood coagulation time measurement cartridge according to the present invention. (A) is a plan view, (b) is a front view, and (c) is a right side view, with respect to the blood coagulation time measurement cartridge of the first embodiment. FIG. 2A is a cross-sectional view taken along line A-A shown in FIG. 2A and FIG. 2B is a bottom view of the cartridge for measuring blood coagulation time according to the first embodiment. It is a figure showing a modification of a 1st embodiment. FIG. 2A is a cross-sectional view corresponding to A-A shown in FIG. 2A, and FIG. 2B is a bottom view showing the second embodiment of the blood coagulation time measurement cartridge according to the present invention. It is a figure showing a modification of a 2nd embodiment.

  Hereinafter, a first embodiment of a cartridge for measuring blood coagulation time (hereinafter, referred to as “cartridge”) according to the present invention will be described with reference to FIGS. 1 to 3.

  The cartridge of the present embodiment includes a base 1 that is flat as a whole, and a thin plate-like closing plate 2 that is fixed to the base 1 on the bottom surface side of the base 1.

  The base 1 is formed of a colorless and transparent synthetic resin. In addition, you may form with not only a synthetic resin but glass etc. Moreover, it is not limited to colorless and transparent, and may be colored and transparent in which light is transmitted at least in a detection area described later. Also, the area other than the detection area may not be transparent. Further, although the closing plate 2 is formed of a colorless and transparent synthetic resin (thin sheet), it can be formed of various materials and colors as long as the function of the present invention is satisfied as in the case of the base 1. The connection between the base 1 and the closing plate 2 may be made of an adhesive material, or may be welded using, for example, ultrasonic waves.

  The bottom of the base 1 is provided with a groove extending long relative to the cross-sectional area as shown in FIG. 3 to form a measurement channel 3 with the closing plate 2. One measurement flow channel 3 in the present embodiment is provided at the center in the width direction of the base 1.

  At one end side of the measurement flow path 3, an inlet 4 capable of introducing blood is provided. The inlet 4 is connected to an opening 5 whose outer peripheral surface is cylindrical and whose inner peripheral surface is conical. Further, on the radially outer side of the opening 5, an annular excess blood receiving portion 6 is provided.

  A communication port 7 is provided on the other end side of the measurement flow path 3. The communication port 7 can suction or pressurize the air in the measurement flow path 3 and the blood introduced into the measurement flow path 3 from the injection port 4 by changing the amount of pressing to the diaphragm described later.

  Further, one end side convex portion 8 and the other end side convex portion 9 provided so as to protrude to the bottom surface side are provided in the central portion of the measurement flow path 3, and these enlarge the portion shown in FIG. As shown in the drawing, a narrowed portion (one end side narrowed portion 10 and the other end side narrowed portion 11) in which the measurement flow path 3 is narrowed is formed.

  The base 1 is provided with a cylindrical wall 12 which is generally cylindrical on the opposite side of the opening 5 and the excess blood receiving portion 6 described above. The inner peripheral surface of the cylindrical wall 12 has a small diameter on the bottom surface side, and has a shape in which the top surface side has a large diameter across the part where the diameter is enlarged. The inner space of the cylindrical wall 12 is connected to the communication port 7 on the bottom side. In the present embodiment, the entire inner space of the cylindrical wall 12 is referred to as an air chamber 13.

  A diaphragm 14 for closing the air chamber 13 is provided on the top surface side of the cylindrical wall 12. The diaphragm 14 is formed of, for example, a rubber having a small thickness and elasticity. Further, on the radially outer side of the cylindrical wall 12, an annular holder 15 for inserting the cylindrical wall 12 and fittingly holding the cylindrical wall 12, and for holding the diaphragm 14 between the cylindrical wall 12 and the cylindrical wall 12 is provided. It is provided. Here, when the diaphragm 14 is pushed toward the bottom side, the volume of the air chamber 13 is reduced, so that the air chamber 13 can be pressurized. In the initial state, if the diaphragm 14 is pushed toward the bottom side, the volume of the air chamber 13 is increased by loosening the pressure, so that the air chamber 13 can be depressurized. The air chamber 13 may be depressurized by pulling up the diaphragm 14 toward the top surface side.

  A movable body 16 is disposed between the one end side narrowing portion 10 and the other end side narrowing portion 11 in the measurement flow channel 3. The outer diameter of the movable body 16 is formed to be slightly smaller than the inner diameter of the measurement channel 3, and can move in the measurement channel 3. Further, between the outer peripheral surface of the movable body 16 and the inner peripheral surface of the measurement flow channel 3, a minute gap through which blood can pass is formed. The outer diameter of the movable body 16 is formed to be larger than the gap between the one end side narrowing portion 10 and the other end side narrowing portion 11. Here, the movable body 16 of the present embodiment has a cylindrical shape and is formed of aluminum. The movable body 16 may have a prismatic shape, or may have an elliptical shape in which the thickness of the end portion decreases with respect to the central portion in the longitudinal direction. Moreover, it may be spherical. Also, various materials such as other metals and synthetic resins can be applied to the material of the movable body 16.

  By the way, on the channel wall surface which defines the measurement channel 3 (in the present embodiment, the inner surface of the groove provided at the central portion in the width direction of the base 1), a coagulation accelerator for promoting coagulation of blood is applied. The coagulation accelerator may be applied also to the top surface of the closing plate 2 facing the measurement flow channel 3 to apply the entire surface to the measurement flow channel 3 or to the moving body 16. It is also good.

  Further, on the top surface of the base 1, there is provided an outer edge wall 17 which is located at the outer edge of the base 1 and which surrounds the holder 15 and which is U-shaped in plan view. Further, as shown in FIG. 1, a plate-like portion (shielding portion) 19 is provided between the excess blood receiving portion 6 and the holder 15 to connect the left and right outer edge walls 17 with each other. Furthermore, in the vicinity of the excess blood receiving portion 6 on the longitudinal side of the base 1, there is an uneven shape, so that the cartridge can be set or removed with respect to the blood coagulation time measuring device (hereinafter referred to as "measuring device") The finger hooking part 20 which becomes a site | part which hooks a finger | toe at the time of doing is provided.

  Further, the base 1 and the closing plate 2 have a detection area capable of transmitting light to a predetermined portion of the measurement flow channel 3. In the present embodiment, as shown in FIG. 3A, the one end side detection area 21 located near the one end side narrowed portion 10 and located on the other end side narrowed portion 11 side, and the other end side narrowed portion 11 The other end side detection area 22 located near the one end side narrowed portion 10 is provided. The one end side detection area 21 is located closer to the other end side detection area 22 with respect to the shielding portion 19.

  The cartridge of such a first embodiment can be set in a measuring device (not shown) to measure the coagulation time of blood. Specifically, the side of the elongated cartridge where the diaphragm 14 is located is inserted into the measuring device, and the cartridge is set in the measuring device. In this state, the opening 5 is located outside the measuring device. Further, as shown in FIG. 3A, the measuring apparatus is provided with pressing means S1 capable of pushing the diaphragm 14 by a predetermined moving amount, and detecting means capable of detecting the presence or absence of the moving body 16 by light. There is. The detection means in the present embodiment is constituted by the one end side light source S2 and the one end side light receiving sensor S3 provided so as to sandwich the measurement channel 3 in the one end side detection area 21, and in the other end side detection area 22 The moving body 16 includes the one end side detection area 21 and the other side light source S4 and the other end side light receiving sensor S5, and the moving body 16 blocks the light of the one end side light source S2 and the other end side light source S4. It can be detected whether it exists in the other end side detection area 22 or not. In addition, one end side light source S2 and other end side light source S4 irradiate infrared rays. The positions of the one end side light source S2, the one end side light receiving sensor S3, the other end side light source S4 and the other end side light receiving sensor S5 are opposite to the illustrated example, and the one end side light source S2 and the other end side light source S4 are cartridge One end side light receiving sensor S3 and the other end side light receiving sensor S5 may be provided on the top surface side of the cartridge. Also, a reflective sensor may be used instead of such a transmissive sensor.

  Then, after the cartridge is set in the measuring device, the blood to be measured is injected into the inside of the opening 5 by a pipette or the like. When the blood is injected, the diaphragm 14 is pushed in advance by the pressing means S1 to reduce the volume of the air chamber 13. Here, the injection amount of blood is sufficient to prevent spillage from the opening 5. However, even when a large amount of blood is injected, the excess blood receiving portion 6 is provided radially outside the opening 5, Blood spilled from the opening 5 can be stored in the excess blood receiving portion 6. Furthermore, since the shielding part 19 is provided on the outside of the excess blood receiving part 6, even if blood may overflow over the excess blood receiving part 6, the one end side detection area 21 and the other end side detection area It does not flow into 22.

  Thereafter, the air chamber 13 is depressurized by pulling back the pressing means S1 pushed in, so the air in the measurement channel 3 is sucked from the communication port 7 and the blood injected into the opening 5 is in the measurement channel 3 Can be drawn into. In this state, the blood is drawn until the other end side constriction portion 11 is exceeded. As a result, the moving body 16 moves to the other end side narrowed portion 11 side with the blood. Here, it can be confirmed whether the light from the other end side light source S4 can be detected by the other end side light receiving sensor S5 whether the moving body 16 has moved to the vicinity of the other end side narrowed portion 11 or not. .

  After that, the air chamber 13 is pressurized by pushing the pressing means S1 again, and the air and blood of the measurement flow path 3 are also pressurized accordingly, so the moving body 16 is put on the one-end side constriction portion 10 together with the blood. Move towards. Here, whether or not the moving body 16 has moved to the vicinity of the one end side narrowed portion 10 can be detected by the one end side light source S2 and the one end side light receiving sensor S3 as described above.

  Thus, the air chamber 13 can be pressurized or depressurized by repeating the pushing and pulling back of the pressing means S1, so the air or blood in the measurement channel 3 is pressurized or drawn through the communication port 7, Accordingly, the moving body 16 can be reciprocated with the blood. As a result, the blood in the measurement flow channel 3 is stirred by the moving body 16, and the coagulation promoter applied to the flow channel wall defining the measurement flow channel 3 can be dissolved in blood efficiently and stably. In addition, since the outer diameter of the movable body 16 is formed to be larger than the gap between the one end side narrowing portion 10 and the other end side narrowing portion 11, the one end side narrowing portion 10 and the other end side are reciprocated. It does not go beyond the constriction 11. In addition, when air is taken into the blood injected into the cartridge, air bubbles may be generated in the measurement flow path 3, and in the conventional cartridge, the air bubbles may be erroneously detected as coagulated blood. However, in the present embodiment, since the mobile body 16 is detected by optimizing the threshold of the one-end side light receiving sensor S 3 or the like, it is possible to suppress the influence of air bubbles on blood coagulation time measurement. it can.

  Then, when the coagulation promoting agent is dissolved by the agitation of the blood by the moving body 16, the blood is gradually coagulated, so that the moving body 16 gradually slows down. That is, even if the timing of pushing and pulling back of the pressing means S1 does not change, the cycle of the reciprocation of the moving body 16 detected by the one end light receiving sensor S3 and the other end light receiving sensor S5 in a state where blood is not coagulated When the movement of the moving body 16 is slowed, the cycle of the reciprocation detected by the one-side light receiving sensor S3 or the like changes, so based on the change in the time required for this reciprocation, the blood coagulation time is It can be calculated. As described above, a minute gap through which blood can pass is formed between the outer peripheral surface of the movable body 16 and the inner peripheral surface of the measurement channel 3, and when the blood starts to coagulate in this space, the movable body 16 moves slow. That is, since the cycle in which the moving body 16 reciprocates is longer than that before the blood is coagulated, the blood coagulation time can be derived from the difference in this cycle. In addition, since the gap is small, the movement of the moving body 16 is affected as soon as the blood starts to coagulate. Therefore, it is possible to measure the coagulation time of blood in a short time.

  In the present embodiment, the moving object 16 reciprocating is detected by the one end side light receiving sensor S3 or the like, but the object detected by the one end side light receiving sensor S3 or the like may be blood. In this case, as coagulation proceeds, blood clots become large and block the one end side constriction portion 10 and the other end side constriction portion 11, so that the flow of reciprocating blood is interrupted, The blood detected by the one end light receiving sensor S3 and the other end light source S4 can not be detected. Therefore, the coagulation time of blood can be measured based on the time when the blood can not be detected by the one end light receiving sensor S3 or the other end light source S4. When blood is detected by the one end side light receiving sensor S3 etc., as shown in FIG. 4, the one end side detection area 21 is in the vicinity of the one end side narrowed portion 10 and opposite to the other end side narrowed portion 11 The other end side detection area 22 may be located near the other end side narrowed portion 11 and on the opposite side to the one end side narrowed portion 10.

  Next, a second embodiment of the cartridge according to the present invention will be described. The cartridge of the present embodiment is provided with a stirrer 23 in place of the movable body 16 as shown in FIG. 5 with respect to the cartridge of the first embodiment described above. Further, in the present embodiment, the inner space of the cylindrical wall 12 is a space on the bottom side communicating with the communication port 7 (a small-diameter space located generally at the bottom in the inner space of the cylindrical wall 12) The space on the top side closed by the diaphragm 14 (in the inner space of the cylindrical wall 12, the space expanding generally and the large-diameter space connected thereto) is called an air chamber 13 b. The other parts are basically the same as the cartridge of the first embodiment, so the same reference numerals are given in the drawings and the description will be omitted.

  The stirrer 23 is disposed in the storage chamber 13a. The stirrer 23 is formed of, for example, a magnet or a ferromagnetic material, and can be rotated in the storage chamber 13a by the magnetic field generation unit S6 provided in the measurement device. The stirrer 23 according to the present embodiment has a cylindrical shape, but may be a prismatic shape or an elliptical shape in which the thickness of the end portion decreases with respect to the central portion in the longitudinal direction. Moreover, what provided several blade | wing in the part which becomes disk shape may be used.

  In addition, on the wall surface of the storage chamber (in the present embodiment, on the inner peripheral surface of the cylindrical wall 12, a small diameter inner peripheral surface located on the bottom side in the inner peripheral surface of the cylindrical wall 12), a coagulation accelerator for promoting coagulation of blood is provided. It has been applied. As in the cartridge of the first embodiment, the coagulation accelerator may be applied to the channel wall defining the measurement channel 3 or may be applied to the stirrer 23.

  Also in the cartridge of the second embodiment, it is possible to measure the coagulation time of blood by setting it in a measuring device which is not shown.

  Specifically, as in the cartridge of the first embodiment, after the diaphragm 14 has been pressed in advance by the pressing means S1, blood is injected into the inside of the opening 5 by a dispenser or the like.

  Then, the air chamber 13b is depressurized by pulling back the pressing means S1 pushed in, so the air in the measurement channel 3 is sucked from the communication port 7 through the storage chamber 13a, and the blood injected into the opening 5 is It can be drawn into the measuring channel 3. Here, in the present embodiment, the blood is drawn in such a way as to reach the storage chamber 13 a beyond the other end side constriction portion 11. It should be noted that since there is a correlation between the amount of blood withdrawn and the amount of blood withdrawn by the pressing means S1, it can be judged by the amount of withdrawal of the pressing means S1 whether the blood has been drawn down to the storage chamber 13a. . By the way, in the one end side light receiving sensor S3 and the other end side light receiving sensor S5 in the present embodiment, the threshold value is optimized so as to detect the presence or absence of blood. For this reason, it can be detected whether the light from one end side light source S2 can be detected by one end side light reception sensor S3 whether the blood has moved to the vicinity of the one end side narrowing part 10 or not. Further, whether or not the blood is moved to the vicinity of the other end side narrowed portion 11 can be detected by whether or not the light from the other end side light source S4 can be detected by the other end side light receiving sensor S5. Therefore, the time when the blood is detected by the one end side light receiving sensor S3 and the other end side light receiving sensor S5 is used as a reference of the amount of withdrawal of the pressing means S1, and the blood is drawn to the storage chamber 13a based on the amount of withdrawal from there. It is also good.

  After the blood has been drawn to the storage chamber 13a, the stirrer 23 is rotated by driving the magnetic field generation unit S6. Thus, the coagulation promoter applied to the wall surface of the storage chamber defining the storage chamber 13a can be dissolved in blood efficiently and stably.

  Then, the air chamber 13b is pressurized by pushing the pressing means S1 again while the stirring bar 23 is rotated (the rotation may be stopped). As a result, the blood in the storage chamber 13 a and the measurement flow channel 3 is also pressurized, so that the blood can flow toward the one end side narrowed portion 10. Thus, the blood can be reciprocated within the measurement flow path 3 by repeating the pushing and pulling back of the pressing means S1.

  When the coagulation promoter dissolves due to the stirring of the blood by the stirring element 23, the blood is gradually coagulated, and thereby the blood lumps become large and block the one end side constriction portion 10 and the other end side constriction portion 11. Then, the flow of blood that has been reciprocated is impeded, and the blood that has been detected by the one end light receiving sensor S3 and the other end light source S4 can not be detected. Therefore, the coagulation time of blood can be measured based on the time when the blood can not be detected by the one end light receiving sensor S3 or the other end light source S4.

  Although the cartridge according to the present invention and the measuring device have been described above by showing specific embodiments, the cartridge according to the present invention is not limited to the above-described embodiment, and various modifications may be made within the scope of the claims. Also included are those to which For example, although the storage chamber 13a and the measurement flow channel 3 shown in FIG. 5 are at the same position on the bottom surface side, the storage chamber 13a is positioned on the bottom surface side with respect to the measurement flow channel 3 as shown in FIG. A step may be provided. Since the stirrer 23 is drawn by the magnetic field generating unit S6, even in the cartridge shown in FIG. 5, the stirrer 23 does not move to the outside (measurement flow path 3) of the storage chamber 13a, but like the cartridge shown in FIG. When the step is provided, the stirrer 23 can be more reliably retained in the storage chamber 13a. Also, various methods can be adopted for fixing the diaphragm 14, and for example, an adhesive or the like may be used to fix it to the base 1. Further, the detection area may be provided on only one of the one end side and the other end side.

1: base 2: blockage plate 3: measurement flow channel 4: inlet 5: opening 6: excess blood receiving portion 7: communication port 8: one end side convex portion 9: other end side convex portion 10: one end side narrowed portion 11 : Other end side constriction portion 12: cylindrical wall 13: air chamber 13a: reservoir 13b: air chamber 14: diaphragm 15: holder 16: moving body 17: outer edge wall 19: shield portion 20: finger hook portion 21: one end side detection Area 22: other end side detection area 23: stirrer

The present invention is provided with a measurement flow channel which extends long with respect to a cross-sectional area, an injection port which is provided on one end side of the measurement flow channel and can introduce blood, and is provided on the other end side of the measurement flow channel. A communication port capable of sucking or pressurizing air in the measurement flow channel or blood introduced into the measurement flow channel from the injection port, a movable body disposed in the measurement flow channel and movable in the measurement flow channel A coagulation promoting agent applied to at least one of a flow path wall surface defining the measurement flow path and the movable body, and light can be transmitted to a predetermined portion of the measurement flow path, from the communication port A detection area capable of detecting by light whether or not the moving body or blood reciprocated in the measurement channel along with suction or pressurization of air or blood is detected by light in use the cartridge, and the air chamber communicating with the communication port, the air A diaphragm for closing the chamber and pressurizing or depressurizing the air chamber to reciprocate the moving body and the blood, the diaphragm having a cylindrical shape and a cylindrical wall having an inner space as the air chamber; A cartridge for measuring blood coagulation time, which has an annular shape and is sandwiched between a holder inserted into the cylindrical wall at the radially outer side of the cylindrical wall .

  The present invention is provided with a measurement flow channel which extends long with respect to a cross-sectional area, an injection port which is provided on one end side of the measurement flow channel and can introduce blood, and is provided on the other end side of the measurement flow channel. A communication port capable of sucking or pressurizing air in the measurement flow channel or blood introduced into the measurement flow channel from the injection port, a movable body disposed in the measurement flow channel and movable in the measurement flow channel A coagulation promoting agent applied to at least one of a flow path wall surface defining the measurement flow path and the movable body, and light can be transmitted to a predetermined portion of the measurement flow path, from the communication port A detection area capable of detecting by light whether or not the moving body or blood reciprocated in the measurement channel along with suction or pressurization of air or blood is detected by light Cartridge, and an air chamber communicating with the communication port; A diaphragm for closing the chamber and pressurizing or depressurizing the air chamber to reciprocate the moving body and the blood, the diaphragm having a cylindrical shape and a cylindrical wall having an inner space as the air chamber; A cartridge for measuring blood coagulation time, which has an annular shape and is sandwiched between a holder inserted into the cylindrical wall at the radially outer side of the cylindrical wall.

Claims (9)

A measuring channel which extends long with respect to the cross-sectional area,
An inlet provided at one end of the measurement channel and capable of introducing blood;
A communication port provided on the other end side of the measurement channel, capable of sucking or pressurizing air in the measurement channel or blood introduced into the measurement channel from the inlet;
A movable body disposed in the measurement channel and capable of moving the measurement channel;
A flow path wall surface defining the measurement flow path, and a coagulation promoter applied to at least one of the movable body;
The movable body or blood which can transmit light to a predetermined portion of the measurement flow channel and reciprocates in the measurement flow channel as the air or blood is sucked or pressurized from the communication port is the predetermined portion And a detection area in which light is detected by light.   The cartridge for measuring blood coagulation time according to claim 1, wherein the measurement flow path includes a constriction portion in the vicinity of the detection area.   The cartridge for measuring blood coagulation time according to claim 1 or 2, further comprising: a shield that prevents blood from flowing toward the detection area outside the inlet.   The air chamber according to any one of claims 1 to 3, further comprising an air chamber communicating with the communication port, and a diaphragm closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate the moving body and the blood. The cartridge for measuring blood coagulation time according to Item. A measuring channel which extends long with respect to the cross-sectional area,
An inlet provided at one end of the measurement channel and capable of introducing blood;
A communication port provided on the other end side of the measurement channel, capable of sucking or pressurizing air in the measurement channel or blood introduced into the measurement channel from the inlet;
A storage chamber connected to the communication port for storing blood flowing out from the communication port;
A stirrer, disposed in the storage chamber, for stirring blood in the storage chamber;
A flow path wall surface defining the measurement flow path, a storage chamber wall surface defining the storage chamber, and a coagulation promoter applied to at least one of the stirring bars;
Is light transmittable to a predetermined portion of the measurement channel, and blood reciprocated in the measurement channel along with suction or pressurization of air or blood from the communication port is present at the predetermined portion? And a detection area whose light can detect whether or not it is a cartridge for measuring blood coagulation time.   The cartridge for measuring blood coagulation time according to claim 5, wherein the measurement flow path includes a constriction portion in the vicinity of the detection area.   The cartridge for measuring blood coagulation time according to claim 5 or 6, further comprising: a shield that prevents blood from flowing toward the detection area outside the inlet.   The air chamber according to any one of claims 5 to 7, further comprising: an air chamber communicating with the storage chamber; and a diaphragm closing the air chamber and pressurizing or depressurizing the air chamber to reciprocate blood in the measurement flow path. The cartridge for measuring blood coagulation time according to one item. A blood coagulation time measurement device for setting the blood coagulation time measurement cartridge according to claim 4 or 8, comprising:
A blood coagulation time measuring device provided at a position corresponding to the detection area and comprising detection means capable of detecting the moving body or blood by light, and pressing means capable of pushing or pulling up the diaphragm by a predetermined movement amount.

Images